Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001474-TA|BGIBMGA001474-PA|IPR013025|Ribosomal protein
L25/L23, IPR012678|Ribosomal L23 and L15e, core
(150 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.08 |mrp20||mitochondrial ribosomal protein subunit L23|... 44 1e-05
SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|S... 36 0.003
SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch... 32 0.032
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po... 28 0.69
SPBC16G5.17 |||transcription factor, zf-fungal binuclear cluster... 27 1.6
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 26 2.1
SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomy... 26 2.1
SPAC23C11.09 |||alanine-tRNA ligase |Schizosaccharomyces pombe|c... 26 2.1
SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr... 26 2.8
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 26 2.8
SPBC83.14c |rfc5||DNA replication factor C complex subunit Rfc5 ... 25 4.9
SPAC22E12.05c |rer1||Rer1 family protein|Schizosaccharomyces pom... 25 4.9
SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 25 4.9
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 25 4.9
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 25 6.5
SPBC2G2.14 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 6.5
SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosa... 25 6.5
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 6.5
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 25 6.5
SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces p... 24 8.5
SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces p... 24 8.5
SPCC188.06c |srp54||signal recognition particle subunit Srp54|Sc... 24 8.5
SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosacc... 24 8.5
SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 8.5
>SPAC31A2.08 |mrp20||mitochondrial ribosomal protein subunit
L23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 161
Score = 43.6 bits (98), Expect = 1e-05
Identities = 26/91 (28%), Positives = 46/91 (50%), Gaps = 4/91 (4%)
Query: 18 VFLPNFWMKLVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFK 77
V+ PN L R Q P F + + K+D+++YL+ +Y + VV V+++I GK
Sbjct: 10 VYFPNITFTLCRGLNLQ-PKFAVFRVPLHVNKFDVRDYLQHVYNLKVVSVKSRIYQGKLF 68
Query: 78 KDVVKGYVIKEDDVKVAFVTLPKTMTFKYPD 108
++ + G V + K V + + F YP+
Sbjct: 69 RNQL-GQVKRPQSEKRVIVEMEE--PFVYPN 96
>SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein
L25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 141
Score = 35.9 bits (79), Expect = 0.003
Identities = 17/46 (36%), Positives = 25/46 (54%)
Query: 37 NIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVK 82
N + FH ++ K+ IKN ++K+Y V V + T I KK VK
Sbjct: 79 NTLVFHVHLKANKFTIKNAVKKLYSVDAVKINTLIRPNGTKKAFVK 124
>SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein
L25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 141
Score = 32.3 bits (70), Expect = 0.032
Identities = 16/46 (34%), Positives = 23/46 (50%)
Query: 37 NIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVK 82
N + FH ++ K+ IK + K+Y V V + T I KK VK
Sbjct: 79 NTLVFHVHLKANKFTIKEAVRKLYSVEPVKINTLIRPNGTKKAFVK 124
>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 833
Score = 27.9 bits (59), Expect = 0.69
Identities = 15/70 (21%), Positives = 35/70 (50%)
Query: 79 DVVKGYVIKEDDVKVAFVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKYIDHN 138
D +K + + +D + VT K E +++E+E + + + E+K+ F + + ++
Sbjct: 274 DELKSFDKENNDEHLERVTNDKIADASMLQKAENNVSEQERNIQLISETKRLFLRNLTYS 333
Query: 139 KSRPDIPSWF 148
+ D+ S F
Sbjct: 334 CAEDDLKSLF 343
>SPBC16G5.17 |||transcription factor, zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 2|||Manual
Length = 560
Score = 26.6 bits (56), Expect = 1.6
Identities = 11/40 (27%), Positives = 21/40 (52%)
Query: 95 FVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKY 134
F+T+ +T+ YPD+ +K + ++ + S F KY
Sbjct: 418 FITVSRTLVPNYPDIVQKLGQTSIQLSRLISNSMDCFEKY 457
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 26.2 bits (55), Expect = 2.1
Identities = 15/66 (22%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
Query: 85 VIKEDDVKVAFVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKYIDHNKSRPDI 144
++KE + + K++ KY + E+S +E+E ++ ++ F + + S P
Sbjct: 97 IVKESKEGIT-LNKKKSLEIKYDE--ERSFDEKENDESEFEDGQQGFIPLLVNRNSDPSE 153
Query: 145 PSWFSI 150
S FS+
Sbjct: 154 KSTFSL 159
>SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 735
Score = 26.2 bits (55), Expect = 2.1
Identities = 13/45 (28%), Positives = 21/45 (46%)
Query: 98 LPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKYIDHNKSRP 142
+PK T + + EK EEEE + L+ + + +DH P
Sbjct: 1 MPKNRTGRSREAREKKRKEEEEEIEELNSQIEALSETVDHFAELP 45
>SPAC23C11.09 |||alanine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 959
Score = 26.2 bits (55), Expect = 2.1
Identities = 13/38 (34%), Positives = 19/38 (50%)
Query: 105 KYPDLFEKSINEEEEHAKSLDESKKNFRKYIDHNKSRP 142
K D+ E EEE +++LD +K F +Y K P
Sbjct: 373 KVDDVRELLDEEEESFSRTLDRGEKMFEQYAAAAKKTP 410
>SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 359
Score = 25.8 bits (54), Expect = 2.8
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
Query: 107 PDLFEKSINEEEEHAKSLDESKKNF---RKY 134
PDL E+S +EE + S E K NF RKY
Sbjct: 141 PDLKEESSTDEEMDSLSAAEEKPNFLQKRKY 171
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 25.8 bits (54), Expect = 2.8
Identities = 18/84 (21%), Positives = 37/84 (44%), Gaps = 7/84 (8%)
Query: 44 SMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIK-------EDDVKVAFV 96
S+E ++K + ++ + T +N + KK+ K +++K EDD+
Sbjct: 297 SVEDDSLELKEITPEALDIGQTSLFTTLNQTQVKKEDNKKFLLKEINAKLNEDDIDSELE 356
Query: 97 TLPKTMTFKYPDLFEKSINEEEEH 120
K T ++ + ++EE EH
Sbjct: 357 IEVKPKTTALDNIEKSKLSEENEH 380
>SPBC83.14c |rfc5||DNA replication factor C complex subunit Rfc5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 358
Score = 25.0 bits (52), Expect = 4.9
Identities = 27/98 (27%), Positives = 43/98 (43%), Gaps = 7/98 (7%)
Query: 8 IYQRGNPQLRVFLPNFWMKLVRPHPKQLP-NIVHFHCSMEMTKYDIKNYLEKIYEVPVVD 66
+Y G+ +L++ F + P K+L NIV +E+T D+ NY I + + D
Sbjct: 58 LYGPGSEKLKIDQRTF----LTPSSKKLQINIVSSLHHLEITPSDVGNYDRVIMQELLKD 113
Query: 67 VRTKINMGKFKKDVVKGYVIKEDD--VKVAFVTLPKTM 102
V + K + K VI D + A L +TM
Sbjct: 114 VAQSAQVDLQAKKIFKVVVINVADELTRDAQAALRRTM 151
>SPAC22E12.05c |rer1||Rer1 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 4.9
Identities = 11/36 (30%), Positives = 20/36 (55%)
Query: 29 RPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPV 64
RP ++LP ++ SM T + + +I++VPV
Sbjct: 107 RPFIRRLPEFKFWYSSMRATLFALVASFFRIFDVPV 142
>SPBC30B4.02c |||R3H and G-patch domain, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.0 bits (52), Expect = 4.9
Identities = 12/51 (23%), Positives = 25/51 (49%)
Query: 97 TLPKTMTFKYPDLFEKSINEEEEHAKSLDESKKNFRKYIDHNKSRPDIPSW 147
T K TF + S+ EE++ + DE + ++ + K +P+I ++
Sbjct: 245 TTDKMTTFTMNKFADLSVLEEDDDDEDEDEELEGEKEEEEEEKEKPEISNY 295
>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 878
Score = 25.0 bits (52), Expect = 4.9
Identities = 11/42 (26%), Positives = 22/42 (52%)
Query: 21 PNFWMKLVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEV 62
P+ + LV +P+ +PN+ +++ +YDI L Y +
Sbjct: 605 PSSKVNLVDTYPQTIPNVSKPSTPIDVPEYDISACLPGFYRL 646
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 24.6 bits (51), Expect = 6.5
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 65 VDVRTKINMGKFKKDVVKGYVIKEDDVKVA 94
V+ R ++N+G V K Y IKED K A
Sbjct: 263 VESRPELNVGTIDFKVGKDYWIKEDPPKPA 292
>SPBC2G2.14 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 533
Score = 24.6 bits (51), Expect = 6.5
Identities = 12/37 (32%), Positives = 20/37 (54%)
Query: 94 AFVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKKN 130
+F + T T + P F K+ ++ + S+DESK N
Sbjct: 406 SFESYHNTQTIQSPMKFTKATPLKDNESASVDESKVN 442
>SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 459
Score = 24.6 bits (51), Expect = 6.5
Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 7/65 (10%)
Query: 70 KINMGKFKKDVVKGYVIKEDDVKVAFVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKK 129
++ G F KD+ G + E A V K+ T D F K H L+ ++K
Sbjct: 310 RLQYGLFLKDI--GLSVDE-----ALVFWRKSFTNVTEDKFNKEYRYNIRHTYGLEGNRK 362
Query: 130 NFRKY 134
N++ Y
Sbjct: 363 NYKGY 367
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 24.6 bits (51), Expect = 6.5
Identities = 9/28 (32%), Positives = 18/28 (64%)
Query: 117 EEEHAKSLDESKKNFRKYIDHNKSRPDI 144
++E S + +KNFR+ ++ + S P+I
Sbjct: 110 KQEQLDSFEILRKNFRETLEESSSNPNI 137
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 24.6 bits (51), Expect = 6.5
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Query: 113 SINEEEEHAKSLDESKKNFRKY-IDHNKSRPDIPS 146
SI+ EEH ++D+S R Y +H+ D PS
Sbjct: 319 SISSAEEHPSNIDDSSIERRHYDSNHSSQIRDHPS 353
>SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 472
Score = 24.2 bits (50), Expect = 8.5
Identities = 11/33 (33%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Query: 112 KSINEEEEHAKSLDESKKNFRKYIDHNKSRPDI 144
KS EE++H + ++ KK+ +Y D K + I
Sbjct: 143 KSKEEEKKHMREFEQIKKSAMRY-DRQKQKEKI 174
>SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1028
Score = 24.2 bits (50), Expect = 8.5
Identities = 8/29 (27%), Positives = 20/29 (68%)
Query: 106 YPDLFEKSINEEEEHAKSLDESKKNFRKY 134
+ D F++ ++E+ K ++++K+NF K+
Sbjct: 304 FEDCFDQFGVYDKEYEKKIEKTKRNFPKF 332
>SPCC188.06c |srp54||signal recognition particle subunit
Srp54|Schizosaccharomyces pombe|chr 3|||Manual
Length = 522
Score = 24.2 bits (50), Expect = 8.5
Identities = 10/21 (47%), Positives = 13/21 (61%)
Query: 119 EHAKSLDESKKNFRKYIDHNK 139
EH +SLD KKN K ++ K
Sbjct: 305 EHVQSLDFDKKNMVKNLEQGK 325
>SPBC2F12.05c |||sterol binding ankyrin repeat
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1310
Score = 24.2 bits (50), Expect = 8.5
Identities = 13/41 (31%), Positives = 20/41 (48%)
Query: 107 PDLFEKSINEEEEHAKSLDESKKNFRKYIDHNKSRPDIPSW 147
P L EK++ ++ A S + R+ +D N RP I W
Sbjct: 899 PSLDEKTMQLAKQIAVSFHGYEAPTRENLDVNDDRPKISLW 939
>SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 600
Score = 24.2 bits (50), Expect = 8.5
Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 16/75 (21%)
Query: 71 INMGKF-KKDVVKGYVIKEDDVKVAFVTLPKTMTFKYPDLFEKSINEEEEHAKSLDESKK 129
IN G + KKD K + K+ D T ++ + DL EE H K +K
Sbjct: 359 INSGLYLKKDAAKNLLAKQFDA---------TYSYYHKDL------EESVHQKLNSNLEK 403
Query: 130 NFRKYIDHNKSRPDI 144
KYI H+ S+ ++
Sbjct: 404 RVEKYIKHSCSQRNV 418
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.321 0.138 0.426
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 815,948
Number of Sequences: 5004
Number of extensions: 35535
Number of successful extensions: 142
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 122
Number of HSP's gapped (non-prelim): 26
length of query: 150
length of database: 2,362,478
effective HSP length: 67
effective length of query: 83
effective length of database: 2,027,210
effective search space: 168258430
effective search space used: 168258430
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 50 (24.2 bits)
- SilkBase 1999-2023 -