Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001472-TA|BGIBMGA001472-PA|IPR004842|Na-K-Cl
cotransporter superfamily, IPR002443|Na-K-Cl co-transporter,
IPR004841|Amino acid permease-associated region
         (1036 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyc...   184   6e-47
SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyce...    34   0.12 
SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p...    33   0.21 
SPAC630.05 |gyp7||GTPase activating protein Gyp7 |Schizosaccharo...    32   0.47 
SPAP7G5.06 |||amino acid permease, unknown 4|Schizosaccharomyces...    31   0.83 
SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces ...    31   1.1  
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c...    29   3.3  
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S...    28   5.8  
SPBC36.03c |||spermidine family transporter |Schizosaccharomyces...    28   7.7  
SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c...    28   7.7  

>SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1050

 Score =  184 bits (448), Expect = 6e-47
 Identities = 96/274 (35%), Positives = 158/274 (57%), Gaps = 8/274 (2%)

Query: 98  TEEGVLIPCLLNIWGVMLFLRISWVVSQAGIGWSLVIIALSAVVCVITTLSMSAICTNGE 157
           T EG  IP  LN+  ++L+LR  W++ +AG+  +L+++ +S  V + T+LS+SAICTNG 
Sbjct: 79  TFEGCFIPTTLNVLSILLYLRFPWIIGEAGVLKTLLMLFISYAVGIFTSLSISAICTNGM 138

Query: 158 VKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSMNHLL---KSLDLQII 214
           V+GGG YY +SRS+GPE G S+G+IF     +   MN  GF + +  +         Q +
Sbjct: 139 VRGGGAYYAVSRSIGPELGGSIGLIFYVGQILNTGMNISGFVEPIISIFGKESGTISQFL 198

Query: 215 DNSYNDVRIIGAIALFVMCVICAVGMDWESKAQNFLIAIIVGAIVDFVVGAV-MGPKSNL 273
              Y  V +     L + C++C +G    +KA N L  +I+ + +   + ++ + P  + 
Sbjct: 199 PEGYWWVFLYTTCVLAMCCILCCLGSAIFAKASNALFVVIILSTISIPISSIFVHPFKDP 258

Query: 274 EVAEGFVGLSTSTFVENFNSDFKYSE---GMEQNFFSVFAIFFPSVTGIQAGANISGDLK 330
            +   F GL  ST ++N  S +  +E   G E +F S F +FFP+  G+ AGA++SGDLK
Sbjct: 259 SLLVHFTGLKWSTLMKNLASAYTENEKGTGYE-SFKSTFGVFFPATAGLLAGASMSGDLK 317

Query: 331 DPASAIPKGTLLALLISMVSYAMMVLFTGAAALR 364
            P+ +IPKGT+ +   + + Y +++L  GA+  R
Sbjct: 318 APSRSIPKGTISSQATTFLLYLLVILCVGASVTR 351



 Score =  105 bits (251), Expect = 4e-23
 Identities = 70/322 (21%), Positives = 145/322 (45%), Gaps = 7/322 (2%)

Query: 383 YSAVSQCANSTLFPCKYGMHVDFEIMQLMSAWGPFIYAGCWXXXXXXXXXXXXXVPRLIQ 442
           Y  V  C  +++   + G+ +D ++M+ +S    FI +G                 +L+Q
Sbjct: 338 YLLVILCVGASV--TRTGLLLDMDVMEHISLHPLFIISGILSSGAFSSFMGIFGAAKLLQ 395

Query: 443 ALGVDRIYPGLIFFSKPYGRHGEAYRGYXXXXXXXXXXXXIAKLNAIAPLISNFYLASYA 502
           A+  D + PG+ FF+K        Y                  +N ++ +I+  +L ++ 
Sbjct: 396 AIARDDLIPGMFFFAKGSSYDDIPYVAIGVTYLITQISL-FWDINMLSSMITMTFLLTFG 454

Query: 503 LINFCTFHAALVRPLGWRPTFKYYNVWVSLAGFLMCVGIMLLISWIMSLVTIAIFFTLYL 562
            IN   F   +     +RPTF+Y+N   +L G ++  G+M  +  + + ++  I   L +
Sbjct: 455 FINLSCFLLRISSTPNFRPTFRYFNRRTTLVGTILSFGVMFYVDRLNAFISFLIAGILVV 514

Query: 563 IVHYRNPDVNWGSSTQAQMYKTALSSAHNLARTGEHVKNYWPQLLVLGGRAHARPPLVDL 622
           ++++  P  NWG  +Q  +Y           +  E++K + PQ+L+L    +    ++  
Sbjct: 515 VIYFTCPPKNWGDVSQGIIYHQLRKYLLQTNKARENIKFWRPQILLLINNPNRSENVIRF 574

Query: 623 GSLITKAGSLMIIGDI---SKEKLSYKVCSARARADNEWLQERKVRAFCSLVHGFNFEQG 679
            + + K GSL I+G +      + S      + R  ++++ +R ++AF  L    +   G
Sbjct: 575 CNSL-KKGSLYILGHVIVSDDFQASMDDLRKQQRLWHQFVLDRGIKAFVELTIAPDEVWG 633

Query: 680 ARALIQATGVGKLAPNVLLMGY 701
            R LI + G+G + PN+ ++ +
Sbjct: 634 IRGLISSAGLGGIRPNIAVLTF 655


>SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 554

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 50/242 (20%), Positives = 106/242 (43%), Gaps = 29/242 (11%)

Query: 130 WSLVIIALSAVVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAV 189
           W   IIA+   + +I+  +M+ +C+      G +Y+  S + G ++G  +G I A+   V
Sbjct: 81  WITYIIAIP--LQLISAATMAEVCS-ALPTAGSLYFWASAAGGKKYGRLIGFIVAWWVVV 137

Query: 190 AASMNTIGFCDSMNHLLKSLDLQIIDNSY----NDVRI------IGAIALFVMCVICAVG 239
           A +      C S    +   +L + ++ +    +DV+       +G   L V  ++  + 
Sbjct: 138 AWTSFVAVNCQSTTKFIFG-ELPVFNSGFSVSSSDVKFRAVQWAVGEAILLVCVLLNFIP 196

Query: 240 MDWESKAQNFLIAIIVGAIVDFVVGAVMGPKSNLEVAEGFVGLSTSTFVENFNSDFKYSE 299
             W        +A+I   ++DFV+  +  P   + V+  + G     F+++ N    Y  
Sbjct: 197 PKWFRYIFRVSVAVI---LLDFVLNMIWLP---IAVSTKY-GFRDEAFMKSTN----YDL 245

Query: 300 GMEQNFFSVFAIFFPS---VTGIQAGANISGDLKDPASAIPKGTLL-ALLISMVSYAMMV 355
           G   N +S    FF +   + G  A  +++ + K+ +    +G    A   +++S  ++V
Sbjct: 246 GKVNNGWSWCLTFFCTARILVGYDAAGHVAEETKNASKTASRGMFYSAFSNAILSTGIIV 305

Query: 356 LF 357
           +F
Sbjct: 306 MF 307


>SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 591

 Score = 33.1 bits (72), Expect = 0.21
 Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 5/76 (6%)

Query: 117 LRISWVVSQAGIG--WSLVIIALSAVVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPE 174
           L  SW +S  G    WS +++  +    ++T   ++ IC+      G IY   + S GP 
Sbjct: 98  LTFSWGISFGGPAAYWSAMLV--TGFCSIVTAACLAEICS-ALPAAGSIYLWAAESAGPR 154

Query: 175 FGASVGIIFAFANAVA 190
           FG  V  + A+ +  A
Sbjct: 155 FGRFVSFLVAWWSTTA 170


>SPAC630.05 |gyp7||GTPase activating protein Gyp7
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 743

 Score = 31.9 bits (69), Expect = 0.47
 Identities = 13/50 (26%), Positives = 28/50 (56%)

Query: 754 LTVTSSGSGELHVRRSDALIMHADSDLDIHTDSSAKNNLSNILTLSTSRS 803
           +T     + ++H+  S   ++ + S + +H  S  K+N+S  L+LS S++
Sbjct: 12  MTEVEDDTVDIHIDNSKVALLFSKSKVFVHPTSKMKDNISGYLSLSKSKA 61


>SPAP7G5.06 |||amino acid permease, unknown 4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 583

 Score = 31.1 bits (67), Expect = 0.83
 Identities = 48/251 (19%), Positives = 98/251 (39%), Gaps = 25/251 (9%)

Query: 112 GVMLFLRISWVVSQAGIGWSLVIIALSAVVCVITTLSMSAICTNGEVKGGGIYYIISRSL 171
           G  LF+     ++  G    ++   L  ++   T  ++  +  +  V GG   Y + R +
Sbjct: 99  GTGLFVGSGSSLADGGPASVIIDYTLIGIMMFFTVYALGELAVSYPVAGGFYNYAV-RFI 157

Query: 172 GPEFGASVGIIFAFANAVAASMNTIGFCDSMNHLLKSLDLQIIDNSYNDVRIIGAIALFV 231
            P +G +VG  + F N        +  C             I    + D+     I +F+
Sbjct: 158 DPAWGFAVGWNY-FMNYFVTFPLELTTC------------AITFRYWTDINSCAWITIFL 204

Query: 232 MCVICA--VGMDWESKAQNFLIAIIVGAIVDFVVGAVMGPKSNLEV-AEGFVGLSTSTFV 288
           + VIC    G+    + +  L  + V A   F++ A++     +     G++G      +
Sbjct: 205 VFVICINLFGVRGYGEVEFILSTLKVVATTGFIILAIIINCGGVPTDPRGYIG---GKII 261

Query: 289 ENFNSDFKYSEGMEQNFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISM 348
           +N    F++S    + F SVF     S +G +     + + +DP  ++P+ T        
Sbjct: 262 KN--KPFRHSF---KGFCSVFTTAGFSFSGTEVVGLAAAEAEDPQKSLPRATKQVFWRIA 316

Query: 349 VSYAMMVLFTG 359
           + Y + ++  G
Sbjct: 317 IFYVVSLILIG 327


>SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 580

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 53/250 (21%), Positives = 106/250 (42%), Gaps = 22/250 (8%)

Query: 125 QAGIGWSLVIIALSAVVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFA 184
           + G    L+  +L   + ++T  S+  +     + G    Y  +R + P +G ++G    
Sbjct: 111 EGGAASVLICYSLVGSMVLMTVYSLGELAVAFPINGSFHTYG-TRFIHPSWGFTLGW--- 166

Query: 185 FANAVAASMNTIGFCDSMNHLLKSLDLQIIDNSYNDVRIIGAIALFVMCVICAVGMDWES 244
             N +A+ + T      +  +  S+ LQ   N  + + I   IAL  +C +   G+    
Sbjct: 167 --NYLASFLATY----PLELITASICLQFWININSGIWITVFIAL--LCFVNMFGVRGYG 218

Query: 245 KAQNFLIAIIVGAIVDFVV-GAVMGPKSNLEVAEGFVGLSTSTFVENFNSDFKYSEGMEQ 303
           + + F+ ++ V A+V F++ G V+          G++G   + F +N      +  G   
Sbjct: 219 EVEFFVSSLKVMAMVGFIICGIVIDCGGVRTDHRGYIG--ATIFRKN-----AFIHGFH- 270

Query: 304 NFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYAMMVLFTGAAAL 363
            F SVF+    S  G +     + + K+PA A PK  +  + I +  + ++ LF  +  +
Sbjct: 271 GFCSVFSTAAFSYAGTEYIGIAASETKNPAKAFPK-AVKQVFIRVSLFYILALFVVSLLI 329

Query: 364 RDASGNITDL 373
                 +T L
Sbjct: 330 SGRDERLTTL 339


>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 796

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 21/65 (32%), Positives = 31/65 (47%)

Query: 561 YLIVHYRNPDVNWGSSTQAQMYKTALSSAHNLARTGEHVKNYWPQLLVLGGRAHARPPLV 620
           YL V++    V  GS++  ++ K   S AH LA    H   ++  L+VL G       L+
Sbjct: 540 YLFVNFFLVYVIAGSTSIWELAKDTTSFAHFLANRLPHQAQFFIDLIVLQGIGMFPLKLI 599

Query: 621 DLGSL 625
            LG L
Sbjct: 600 QLGKL 604


>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
           Cap1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 551

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%)

Query: 543 LLISWIMSLVTIAIFFTLYLIVHYRNPDVNWGSSTQAQMYKTALSS 588
           L I W+ S +T+      Y+  H++   + W +   A   KTAL++
Sbjct: 242 LQIEWVRSYLTLLTELITYVKTHFKT-GLTWSTKQDAVPLKTALAN 286


>SPBC36.03c |||spermidine family transporter |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 538

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 23/112 (20%), Positives = 48/112 (42%), Gaps = 10/112 (8%)

Query: 111 WGVMLFLRISWV-VSQAGIGWSLVIIALSAVVCVITTLSMSAICTNGEVKGGGIYYIISR 169
           WG  +F   + +  ++  IG ++ ++ +S  VC   +         G +    I  ++ R
Sbjct: 112 WGSSVFSAAATIFAAKYHIGLTVALLGMSLYVCGFAS---------GPILWAPISELVGR 162

Query: 170 SLGPEFGASVGIIFAFANAVAASMNTIGFCDSMNHLLKSLDLQIIDNSYNDV 221
            +    G  +  IF+ A AVA  + T+  C   +    S  L ++  ++ D+
Sbjct: 163 KIPLIVGMFMFSIFSIAVAVAKDVQTVMICRFFSGFCASSPLSVVAAAFADM 214


>SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 21/64 (32%), Positives = 31/64 (48%), Gaps = 10/64 (15%)

Query: 126 AGIGWSLVI---------IALSAVVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFG 176
           AGIG  LVI         I  + +    TT+ +S + T+G + GGG   +I+   G   G
Sbjct: 362 AGIGGGLVIGLSSGLLAPIISAGIGAAFTTVGLSGVATSGFLAGGGSAALITAG-GAISG 420

Query: 177 ASVG 180
           A +G
Sbjct: 421 AHIG 424


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.323    0.136    0.410 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,088,377
Number of Sequences: 5004
Number of extensions: 152614
Number of successful extensions: 392
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 382
Number of HSP's gapped (non-prelim): 13
length of query: 1036
length of database: 2,362,478
effective HSP length: 80
effective length of query: 956
effective length of database: 1,962,158
effective search space: 1875823048
effective search space used: 1875823048
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 59 (27.9 bits)