BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001467-TA|BGIBMGA001467-PA|IPR009036|Molybdenum cofactor biosynthesis, IPR006285|E1-like protein-activating enzyme Gsa7p/Apg7p, IPR000594|UBA/THIF-type NAD/FAD binding fold (681 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 464 e-131 SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 49 2e-06 SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 46 2e-05 SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 38 0.005 SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 36 0.024 SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 32 0.23 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 30 0.92 SPAC1F8.04c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Manual 30 0.92 SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizo... 29 2.1 SPAC9.04 |Tf2-1||retrotransposable element|Schizosaccharomyces p... 28 3.7 SPBC9B6.02c |Tf2-9|SPBC9B6.02|retrotransposable element|Schizosa... 28 3.7 SPAC27E2.08 |Tf2-6||retrotransposable element|Schizosaccharomyce... 28 3.7 SPAC2E1P3.03c |Tf2-3||retrotransposable element|Schizosaccharomy... 28 3.7 SPAC26A3.13c |Tf2-4||retrotransposable element|Schizosaccharomyc... 28 3.7 SPCC1020.14 |Tf2-12||retrotransposable element|Schizosaccharomyc... 28 3.7 SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 28 3.7 SPAPB15E9.03c |Tf2-5||retrotransposable element|Schizosaccharomy... 28 3.7 SPAC167.08 |Tf2-2|SPAC1705.01c|retrotransposable element|Schizos... 28 3.7 SPCC61.01c |str2|str1, SPCC622.20c|siderophore-iron transporter ... 28 4.9 SPAC6G9.15c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 6.5 SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 27 6.5 >SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 464 bits (1145), Expect = e-131 Identities = 275/670 (41%), Positives = 389/670 (58%), Gaps = 51/670 (7%) Query: 11 IQYVPFSSFVHPSFWHTLTEMKLEVDKLKETTKQIFGRF-TY-RCDIGSVFEVDGTSFNK 68 +Q+ F S + +FWH L+ K+E KL + I G+F TY R +I VF ++ N Sbjct: 7 LQFQSFHSSIDATFWHQLSNYKVEKQKLDASPLTIHGKFNTYSRGNISIVFGEAPSNSNI 66 Query: 69 TPHLEQQYHHVMGTIMNKNTIEDFKSIDKASLLNSIGEMIWSNLRERTWITNPSALLNFF 128 L + GT++N NT ++F + D + IGE++ ++++ P+ LL F Sbjct: 67 KDCLAE------GTLLNANTPQEFTNADVKKIREEIGEVLLNSIKNGVVSERPNELLRFL 120 Query: 129 ILSFADLKKFHYYYWFAFPAPSQ-PTVHMKGRSTKISDYFNNKQLETLSQCYKSLEENQK 187 I S+AD+K + Y+YW FP+ + P +K S S + L + + + + Q+ Sbjct: 121 IFSYADIKAYKYHYWCLFPSFKETPHWIVKDLSPAESLIPSGPILSQIREFLSTADYYQR 180 Query: 188 NFFVVIKKN-DDLSVKKLSEVFDVNSANCIDLDLVSTYFVFADPSNGCN-PGWPLRTFLA 245 FF++IK D+ ++ L E+ ++C+D L Y V D P WP+R LA Sbjct: 181 PFFLLIKSTLDEWTIAPLKEL-----SHCVDKSL-QFYLVAEDSVQLAEYPSWPVRNILA 234 Query: 246 ALLEYCPELAKSTLQVIGL---RSSMNGDFI-KSLVFSIEIPQDIKPVESAGWVGWERND 301 K L+VI L R +N D + KS++ +E +D+ VGWERN Sbjct: 235 FAF------IKFKLKVINLFLYRDGINSDTLSKSILIKVEADKDMILEAPLSIVGWERNG 288 Query: 302 KGNFGPRLANMSTSMDPVILADTSSDLNIKLMKWRLVPDLNVGVMKDTKCLLLGAGTLGC 361 KG GPR+ N+ST +DP +L++++S LN+ LM+WRLVP L++ ++++KCLLLGAGTLGC Sbjct: 289 KGVLGPRVVNLSTVLDPFVLSESASTLNLSLMRWRLVPQLDLDRIQNSKCLLLGAGTLGC 348 Query: 362 HVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKAEAAADNLKSILPTT 421 VARNLL+WG RH+TFVD VSYSNP RQ LF ++DC KAE AA LK I P Sbjct: 349 GVARNLLSWGVRHVTFVDYSTVSYSNPVRQSLFTFEDC-KRKLPKAECAAQRLKEIYPNM 407 Query: 422 NSKGIVAHIPMPGHPIGDSLKEETIGDIKRITEAISEHDVVFLLLDTREARWLPTLIAAQ 481 S G IPM GHPI ++ E+T+ D + + IS HD +FLL DTRE+RWLPT+I+ Sbjct: 408 FSTGYNISIPMLGHPIYEAGIEKTMHDYETLENLISTHDAIFLLTDTRESRWLPTVISTA 467 Query: 482 HRKIVINAALGFDSYLVMRHGISTSSEEVGTLDKQYIEGRYLGCYFCNDVTAPGNSLRDR 541 K++IN+ALGFDS+LVMRHG L K E R LGCYFCND+ AP NSL DR Sbjct: 468 MDKLLINSALGFDSWLVMRHG--------SVLQK---ENR-LGCYFCNDIFAPSNSLVDR 515 Query: 542 TLDQQCTVTRPGVAAVAGALSVEILVALLQHPKRVDAPALYNFNKTEQEIPSQIEGVLGA 601 TLDQ CTVTR G A +A A++VE+ V+LLQHP AP L N ++T VLG Sbjct: 516 TLDQTCTVTRSGCANIATAIAVELFVSLLQHPNGHAAPVL-NEDQT----------VLGE 564 Query: 602 VPHSIRGFLHSYQTIAPTCTKFKQCIACSDTVINKYREEGLDFLLNVFNSGSYLEEVTGL 661 +PH IRGFLH++ + + + QC ACS+ +IN++ E F+L N Y+EE+ GL Sbjct: 565 LPHQIRGFLHNFSLMKISGMAYPQCSACSECIINEWNREKWMFVLRAINEPDYVEELCGL 624 Query: 662 SALHLSAEMS 671 + E++ Sbjct: 625 REVQALGEIA 634 >SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 49.2 bits (112), Expect = 2e-06 Identities = 25/73 (34%), Positives = 40/73 (54%), Gaps = 3/73 (4%) Query: 349 TKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKAE 408 +K L++GAG LGC + ++L GFR ++ +D + +N RQ LFN + KA Sbjct: 45 SKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNI---DEPKAN 101 Query: 409 AAADNLKSILPTT 421 AA + +P+T Sbjct: 102 VAASMIMKRIPST 114 >SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 46.0 bits (104), Expect = 2e-05 Identities = 28/84 (33%), Positives = 40/84 (47%), Gaps = 3/84 (3%) Query: 347 KDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRK 406 K K LL+GAG +GC + +NLL G + + +D + SN RQ LF + + K Sbjct: 24 KSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV---KQPK 80 Query: 407 AEAAADNLKSILPTTNSKGIVAHI 430 A AA S P + A+I Sbjct: 81 AIVAAKTASSFNPNVKLEAYHANI 104 >SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme |Schizosaccharomyces pombe|chr 2|||Manual Length = 1012 Score = 37.9 bits (84), Expect = 0.005 Identities = 24/77 (31%), Positives = 37/77 (48%), Gaps = 8/77 (10%) Query: 353 LLGAGTLGCHVARNLLAWGFR-----HITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKA 407 L+GAG +GC + +N G HI+ D + SN RQ LF +D G+ K+ Sbjct: 432 LVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIEKSNLNRQFLFRPRDV---GKLKS 488 Query: 408 EAAADNLKSILPTTNSK 424 E A+ + + P+ K Sbjct: 489 ECASTAVSIMNPSLTGK 505 >SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 35.5 bits (78), Expect = 0.024 Identities = 23/74 (31%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405 +K + L++GAG LGC + L+A G + +D V SN RQ++ + G Sbjct: 41 LKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSNLHRQIIHSTSK---QGMH 97 Query: 406 KAEAAADNLKSILP 419 KA +A L+ + P Sbjct: 98 KAISAKQFLEDLNP 111 >SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 32.3 bits (70), Expect = 0.23 Identities = 21/75 (28%), Positives = 31/75 (41%), Gaps = 4/75 (5%) Query: 345 VMKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGR 404 + K CLL A T+GC +NL+ G VD+ V +S Y G+ Sbjct: 25 IEKSHVCLLY-ANTVGCEALKNLILPGIGSFAVVDDTSVDFSMDGMNFFIQYDQ---EGK 80 Query: 405 RKAEAAADNLKSILP 419 +A A L+ + P Sbjct: 81 SRARCTASLLQQLNP 95 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 30.3 bits (65), Expect = 0.92 Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%) Query: 149 PSQPTVHMKGRSTKISDYFNNK-QLETLSQCYKSLEENQKNFFVVIKKNDDLS 200 P PT+H K I D FN + E+L + KS Q ++ KND+ S Sbjct: 379 PPSPTIHTKAALADILDIFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENS 431 >SPAC1F8.04c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 463 Score = 30.3 bits (65), Expect = 0.92 Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 2/50 (4%) Query: 387 NPTRQVLFNYQDCLGGGRRKAE--AAADNLKSILPTTNSKGIVAHIPMPG 434 NPTR +L + + G + D L SI P K + HI MPG Sbjct: 12 NPTRDILIDGAILVKDGSNTIDDIGKTDRLVSIYPNEKHKSLEGHIVMPG 61 >SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizosaccharomyces pombe|chr 1|||Manual Length = 801 Score = 29.1 bits (62), Expect = 2.1 Identities = 26/119 (21%), Positives = 50/119 (42%), Gaps = 8/119 (6%) Query: 137 KFHYYYWFAFPAPSQPTVHMKGRSTKISDYFNNKQLETLSQCYKSLEENQKNFFVVIKKN 196 K+H + A P P P+V++ G F + ++ L +K ++ + ++ K Sbjct: 492 KYHLLDFHALPLPLDPSVNIVGIIPDACTVFKS-TMQPLRLLFKC--QDGSKYPIIFKNG 548 Query: 197 DDLS-----VKKLSEVFDVNSANCIDLDLVSTYFVFADPSNGCNPGWPLRTFLAALLEY 250 DDL ++ L+ + + +DL L + P++G P +T L EY Sbjct: 549 DDLRQDQLVIQILTLMDKLLKKEKLDLHLKPYRILATGPTHGAVQFVPSKTLATILAEY 607 >SPAC9.04 |Tf2-1||retrotransposable element|Schizosaccharomyces pombe|chr 1|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPBC9B6.02c |Tf2-9|SPBC9B6.02|retrotransposable element|Schizosaccharomyces pombe|chr 2|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPAC27E2.08 |Tf2-6||retrotransposable element|Schizosaccharomyces pombe|chr 1|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPAC2E1P3.03c |Tf2-3||retrotransposable element|Schizosaccharomyces pombe|chr 1|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPAC26A3.13c |Tf2-4||retrotransposable element|Schizosaccharomyces pombe|chr 1|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPCC1020.14 |Tf2-12||retrotransposable element|Schizosaccharomyces pombe|chr 3|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 781 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/39 (38%), Positives = 20/39 (51%) Query: 252 PELAKSTLQVIGLRSSMNGDFIKSLVFSIEIPQDIKPVE 290 P A S +G S NG+ +SL S E+P KP+E Sbjct: 202 PRRANSASHNLGSTKSPNGNAKESLSRSAELPSKAKPME 240 >SPAPB15E9.03c |Tf2-5||retrotransposable element|Schizosaccharomyces pombe|chr 1|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPAC167.08 |Tf2-2|SPAC1705.01c|retrotransposable element|Schizosaccharomyces pombe|chr 1|||Manual Length = 1333 Score = 28.3 bits (60), Expect = 3.7 Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 462 VFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTS 506 +F LD + A L + K+ G YLVM +GIST+ Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTA 541 >SPCC61.01c |str2|str1, SPCC622.20c|siderophore-iron transporter Str2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 597 Score = 27.9 bits (59), Expect = 4.9 Identities = 25/70 (35%), Positives = 34/70 (48%), Gaps = 6/70 (8%) Query: 65 SFNKTPHLEQQYHHVMGTIMNKNTIEDFKSIDKASLLN-SIGEMIWSNLRERTWITNPSA 123 SF +LEQQ+H + + N I D S + SL S E I S +R+ I A Sbjct: 14 SFRNNENLEQQFHPLREELDNAGPINDRTS--ELSLEGVSKAEAIASTWSKRSIIV---A 68 Query: 124 LLNFFILSFA 133 L ++LSFA Sbjct: 69 YLGLYLLSFA 78 >SPAC6G9.15c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 27.5 bits (58), Expect = 6.5 Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 1/47 (2%) Query: 79 VMG-TIMNKNTIEDFKSIDKASLLNSIGEMIWSNLRERTWITNPSAL 124 +MG T +KN +ED KAS +SI M+ + E + + N S+L Sbjct: 156 IMGVTKSSKNLVEDAHKSKKASTNSSINNMVSTVNSENSNVNNGSSL 202 >SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Manual Length = 918 Score = 27.5 bits (58), Expect = 6.5 Identities = 13/35 (37%), Positives = 19/35 (54%) Query: 55 IGSVFEVDGTSFNKTPHLEQQYHHVMGTIMNKNTI 89 I S+F + F LE+QYHHV+ ++ N I Sbjct: 660 ISSIFWIAHHVFANDALLEKQYHHVLYSLANTYVI 694 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.320 0.135 0.408 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,194,752 Number of Sequences: 5004 Number of extensions: 138873 Number of successful extensions: 378 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 349 Number of HSP's gapped (non-prelim): 30 length of query: 681 length of database: 2,362,478 effective HSP length: 78 effective length of query: 603 effective length of database: 1,972,166 effective search space: 1189216098 effective search space used: 1189216098 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 57 (27.1 bits)
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