BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001467-TA|BGIBMGA001467-PA|IPR009036|Molybdenum cofactor biosynthesis, IPR006285|E1-like protein-activating enzyme Gsa7p/Apg7p, IPR000594|UBA/THIF-type NAD/FAD binding fold (681 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_24400| Best HMM Match : No HMM Matches (HMM E-Value=.) 135 1e-31 SB_4956| Best HMM Match : ThiF (HMM E-Value=0) 52 1e-06 SB_22173| Best HMM Match : No HMM Matches (HMM E-Value=.) 52 1e-06 SB_2839| Best HMM Match : No HMM Matches (HMM E-Value=.) 36 0.081 SB_57150| Best HMM Match : ThiF (HMM E-Value=2.8e-35) 36 0.11 SB_28306| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.43 SB_54885| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 1.00 SB_27391| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 1.00 SB_19048| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.3 SB_28495| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.7 SB_50262| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.7 SB_21097| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.7 SB_2559| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 3.0 SB_11523| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 5.3 SB_20038| Best HMM Match : wnt (HMM E-Value=3.8e-05) 29 9.3 >SB_24400| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 813 Score = 135 bits (327), Expect = 1e-31 Identities = 84/205 (40%), Positives = 112/205 (54%), Gaps = 29/205 (14%) Query: 219 DLVSTYFVFADPSN-GCNPGWPLRTFLAALLEYCPELAKSTLQVIGLRSSMNG---DFIK 274 DL FADP+ NPGWPLR FL L + L+V+ R G + Sbjct: 455 DLFQLMVGFADPTTLPSNPGWPLRNFLLFLAFHWGTKIDD-LKVLCFRDRFRGGRREIDH 513 Query: 275 SLVFSIEIPQDIKPVESAGWVGWERNDKGNFGPRLANMSTSMDPVILADTSSDLNIKLMK 334 S+V + +P I E ++GWE+N K GPR ++S +MDP LA++S DLN+KLM+ Sbjct: 514 SIVLDVTLPV-INENECPKYIGWEKNKKQKLGPRSVDLSATMDPEKLAESSVDLNLKLMR 572 Query: 335 WRLVPDLNVGVMKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLF 394 WRL+P+L++ V+ T+CLLLGAGTLGC+VAR L+ LF Sbjct: 573 WRLLPELDLDVVSSTRCLLLGAGTLGCNVARCLM-----------------------TLF 609 Query: 395 NYQDCLGGGRRKAEAAADNLKSILP 419 + DC GGR KA AAA+ LK I P Sbjct: 610 EFDDCKEGGRPKAAAAAEALKRIFP 634 Score = 64.9 bits (151), Expect = 2e-10 Identities = 27/63 (42%), Positives = 41/63 (65%) Query: 448 DIKRITEAISEHDVVFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGISTSS 507 D+ R+ + I HDV+FLL+DTRE+RWLPT++AA K V+ A L FD +S ++ Sbjct: 732 DVTRLEQLIESHDVIFLLMDTRESRWLPTVMAAARHKTVLPACLAFDKCTACSSKVSAAN 791 Query: 508 EEV 510 ++ Sbjct: 792 HDL 794 Score = 51.2 bits (117), Expect = 3e-06 Identities = 34/114 (29%), Positives = 56/114 (49%), Gaps = 13/114 (11%) Query: 8 TEIIQYVPFSSFVHPSFWHTLTEMKLEVDKLKETTKQIFGRFT---YRCDIGSVFEVDGT 64 + I+Q+ PFSS V FWH L + KL+ L + K + G + Y + + Sbjct: 280 SRILQFAPFSSAVDAIFWHKLKDKKLDEYYLNDEPKPLQGYYVNCEYLWILLKQTKAKNG 339 Query: 65 SFNKTPHLE----------QQYHHVMGTIMNKNTIEDFKSIDKASLLNSIGEMI 108 SF+ H++ + G ++N NTI+ F+S+DK L++S+GE I Sbjct: 340 SFSFLTHIQWNVKKRNAVPLRAFKTHGQLVNTNTIDAFRSLDKKILMDSVGEKI 393 >SB_4956| Best HMM Match : ThiF (HMM E-Value=0) Length = 213 Score = 52.4 bits (120), Expect = 1e-06 Identities = 29/92 (31%), Positives = 49/92 (53%), Gaps = 3/92 (3%) Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405 + ++K L++GAG +GC + +NL+ GF++I +D + SN RQ LF+ Q G+ Sbjct: 21 VNNSKILVVGAGGIGCELLKNLVLTGFKNIDLIDLDTIDVSNLNRQFLFHKQHV---GKS 77 Query: 406 KAEAAADNLKSILPTTNSKGIVAHIPMPGHPI 437 KA+ A ++ P I +I P + I Sbjct: 78 KAKVARESALRFNPDAKIVAIHDNITSPEYGI 109 >SB_22173| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 200 Score = 52.4 bits (120), Expect = 1e-06 Identities = 29/92 (31%), Positives = 49/92 (53%), Gaps = 3/92 (3%) Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405 + ++K L++GAG +GC + +NL+ GF++I +D + SN RQ LF+ Q G+ Sbjct: 21 VNNSKILVVGAGGIGCELLKNLVLTGFKNIDLIDLDTIDVSNLNRQFLFHKQHV---GKS 77 Query: 406 KAEAAADNLKSILPTTNSKGIVAHIPMPGHPI 437 KA+ A ++ P I +I P + I Sbjct: 78 KAKVARESALRFNPDAKIVAIHDNITSPEYGI 109 >SB_2839| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 529 Score = 36.3 bits (80), Expect = 0.081 Identities = 25/73 (34%), Positives = 40/73 (54%), Gaps = 4/73 (5%) Query: 324 TSSDLNIKLMKWRLVPDLNVG---VMKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDN 380 T+S+++ + + ++P++ V +K + L++GAG LGC A L A G + VD Sbjct: 53 TNSEIS-RYSRQLILPEIGVKGQQSLKSSSVLVVGAGGLGCPAALYLAAAGIGCLGLVDY 111 Query: 381 GKVSYSNPTRQVL 393 V SN RQVL Sbjct: 112 DVVDTSNLHRQVL 124 >SB_57150| Best HMM Match : ThiF (HMM E-Value=2.8e-35) Length = 1026 Score = 35.9 bits (79), Expect = 0.11 Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 3/65 (4%) Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405 M + L+ G LG +A+N++ G + +T D G V S+ T Q N +D G+ Sbjct: 75 MAVSNVLISGLRGLGVEIAKNVVLGGVKSVTLHDTGAVELSDLTSQFFLNKKDV---GKN 131 Query: 406 KAEAA 410 +AE + Sbjct: 132 RAEVS 136 Score = 29.5 bits (63), Expect = 9.3 Identities = 18/54 (33%), Positives = 23/54 (42%), Gaps = 5/54 (9%) Query: 350 KCLLLGAGTLGCHVARNLLAWGF-----RHITFVDNGKVSYSNPTRQVLFNYQD 398 K ++GAG +GC +N G HI D + SN RQ LF D Sbjct: 344 KYFVVGAGAIGCEHLKNFAMMGLGSGEGGHIYTTDMDTIEKSNLNRQFLFRPAD 397 >SB_28306| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 251 Score = 33.9 bits (74), Expect = 0.43 Identities = 22/74 (29%), Positives = 33/74 (44%), Gaps = 1/74 (1%) Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405 M L G G LG +A+NL G + IT D S ++ Q F +D + R Sbjct: 27 MAHASVFLSGVGGLGVEIAKNLTLAGIKSITLHDTRAASMADLGSQ-FFLREDDVTSSRN 85 Query: 406 KAEAAADNLKSILP 419 +A A+A + + P Sbjct: 86 RAVASAGRVAELNP 99 >SB_54885| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2988 Score = 32.7 bits (71), Expect = 1.00 Identities = 21/52 (40%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Query: 145 AFPAPSQPTVHMKGRSTKISDYFNNKQLETLSQCYKSLEENQKNFFVVIKKN 196 AF PS + +K S IS Y NN Q++ LS L N N VV K N Sbjct: 1623 AFRDPSDQPIAIKNLSEPISIYLNNGQIDKLSSLNGELTLNSPN-MVVYKVN 1673 >SB_27391| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 216 Score = 32.7 bits (71), Expect = 1.00 Identities = 16/38 (42%), Positives = 24/38 (63%), Gaps = 2/38 (5%) Query: 89 IEDFKSIDKASLLNSIGEMIWSNLRERTWITNPSALLN 126 + DF SI KAS + I +++ N+ ER+W PS +LN Sbjct: 149 LPDFASIVKASRIKLISQLL--NMEERSWKIIPSCILN 184 >SB_19048| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 611 Score = 32.3 bits (70), Expect = 1.3 Identities = 17/49 (34%), Positives = 27/49 (55%) Query: 190 FVVIKKNDDLSVKKLSEVFDVNSANCIDLDLVSTYFVFADPSNGCNPGW 238 F V KK+D L + +EV V+ +DL + +A+P+NG + GW Sbjct: 98 FTVTKKDDKLPFMEGAEVIVVDDYKPMDLTTDESSGSWANPTNGIDLGW 146 >SB_28495| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 6753 Score = 31.9 bits (69), Expect = 1.7 Identities = 21/68 (30%), Positives = 33/68 (48%), Gaps = 4/68 (5%) Query: 44 QIFGRF-TYRCD--IGSVFEVDGTSFNKTPHLEQQYHHVMGTIMNKNTIEDFKSIDKASL 100 +++G + Y C+ I VF +DGTSF K Q +V T+ + N D + ++ Sbjct: 2863 EMYGPYCNYTCESSIDVVFALDGTSFTKPIEFYQTIQYVRATVNSLNVSSDGTHVG-VTV 2921 Query: 101 LNSIGEMI 108 IGE I Sbjct: 2922 YAKIGERI 2929 >SB_50262| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 830 Score = 31.9 bits (69), Expect = 1.7 Identities = 18/75 (24%), Positives = 40/75 (53%), Gaps = 1/75 (1%) Query: 442 KEETIGDIKRITEAISEHDVVFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRH 501 KE+++ +KR ++ D+ L+ + A ++ T IA + ++N A+ D ++++ Sbjct: 60 KEDSLMSLKRAIIGLAVVDIGGLVFTSPAAEYIATSIAKRPELALVNKAIS-DYKHILKN 118 Query: 502 GISTSSEEVGTLDKQ 516 G++T + L KQ Sbjct: 119 GLATEVADADALIKQ 133 >SB_21097| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 188 Score = 31.9 bits (69), Expect = 1.7 Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Query: 197 DDLSVKKLSEVFDVNSANCIDLDLVSTYFVFADPSNGCNPGWPL 240 DDL K+ S V + +C + L S YF + SN C+PG PL Sbjct: 33 DDLRSKRFSCVSAMRGKSCEVIHLRSLYF--PETSNSCSPGDPL 74 >SB_2559| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1324 Score = 31.1 bits (67), Expect = 3.0 Identities = 20/68 (29%), Positives = 29/68 (42%), Gaps = 3/68 (4%) Query: 435 HPIGDSLKEETIGDIKRITEAISEHDVVFLLLDTREARWLPTLIAAQHRKIVINAALGFD 494 HP+ S+KEET+ +R I EH L WL T + ++I++ Sbjct: 877 HPLFSSMKEETVWSFERAQAYIDEHHTKEKQL---PENWLFTTFTKRMQQIMLTCFHSVR 933 Query: 495 SYLVMRHG 502 L RHG Sbjct: 934 KKLHRRHG 941 >SB_11523| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 411 Score = 30.3 bits (65), Expect = 5.3 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 4/55 (7%) Query: 169 NKQLETLSQCYKSLEENQKNFFVVIKKNDDLSVKKLSEVFDVNSANCIDLDLVST 223 +K ++ S Y S+ E ++ F+ +N V KLS VFD + LDL++T Sbjct: 197 HKAMKDFSNTYPSIHELSRDNFIWNIRNAPRVVNKLSHVFDCG----VKLDLINT 247 >SB_20038| Best HMM Match : wnt (HMM E-Value=3.8e-05) Length = 155 Score = 29.5 bits (63), Expect = 9.3 Identities = 28/95 (29%), Positives = 44/95 (46%), Gaps = 14/95 (14%) Query: 182 LEENQKNFFVVIKKNDDLSVKKLSEVFDVNSANCIDLDLVSTYFVFADPSNGCNPGWPLR 241 L E+ + +++ D+LS+K+ F NC S F S+ PG P Sbjct: 24 LLESYEKLITIVQSGDELSLKECKSQFQNEKWNC------SVRFA----SSNRMPGLPRL 73 Query: 242 TFLAALLEYC-PELAKSTLQVIG-LRSSMNGDFIK 274 AA YC P+ +K + V+G + SS N D++K Sbjct: 74 APSAA--HYCHPDASKGSTGVLGRVCSSDNPDYLK 106 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.320 0.135 0.408 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,517,481 Number of Sequences: 59808 Number of extensions: 934113 Number of successful extensions: 1932 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 1913 Number of HSP's gapped (non-prelim): 23 length of query: 681 length of database: 16,821,457 effective HSP length: 87 effective length of query: 594 effective length of database: 11,618,161 effective search space: 6901187634 effective search space used: 6901187634 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 63 (29.5 bits)
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