Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001467-TA|BGIBMGA001467-PA|IPR009036|Molybdenum cofactor
biosynthesis, IPR006285|E1-like protein-activating enzyme Gsa7p/Apg7p,
IPR000594|UBA/THIF-type NAD/FAD binding fold
(681 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 500 e-141
At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative (... 53 7e-07
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 50 5e-06
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 50 5e-06
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 36 0.12
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 35 0.20
At1g36050.1 68414.m04479 expressed protein 32 1.1
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 32 1.4
At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3... 30 4.4
At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 30 4.4
At1g63390.1 68414.m07168 flavin-containing monooxygenase-related... 30 4.4
At1g51720.1 68414.m05828 glutamate dehydrogenase, putative simil... 30 4.4
At5g37530.1 68418.m04520 thiF family protein similar to SP|P3013... 29 7.7
At4g39030.1 68417.m05528 enhanced disease susceptibility 5 (EDS5... 29 7.7
At2g18810.1 68415.m02189 hypothetical protein contains Pfam prof... 29 7.7
At1g60140.1 68414.m06775 glycosyl transferase family 20 protein ... 29 7.7
>At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to
autophagy 7 [Arabidopsis thaliana] GI:19912147; contains
Pfam profile PF00899: ThiF family
Length = 697
Score = 500 bits (1234), Expect = e-141
Identities = 283/703 (40%), Positives = 393/703 (55%), Gaps = 36/703 (5%)
Query: 1 MLEAQNQTEIIQYVPFSSFVHPSFWHTLTEMKLEVDKLKETTKQIFGRF---------TY 51
M E + I+Q+ P +S V FWH+ + +KL+ + ++ I G + +
Sbjct: 1 MAEKETPAIILQFAPLNSSVDEGFWHSFSSLKLDKLGIDDSPISITGFYGPCGHPQVSNH 60
Query: 52 RCDIGSVFEVDGTSF-NKTPHLEQQYHHVMGTIMNKNTIEDFKSIDKASLLNSIGEMIWS 110
+ +D S T H + V G + N NT+E F +DK SLL + IW
Sbjct: 61 LTLLSESLPLDEQSLIASTSHGNRNKCPVPGILYNTNTVESFNKLDKQSLLKAEANKIWE 120
Query: 111 NLRERTWITNPSALLNFFILSFADLKKFHYYYWFAFPA-PSQPTVHMKGRSTKISDYFNN 169
+++ + +PS L F ++SFADLKK+ + YWFAFPA P V + S+YF++
Sbjct: 121 DIQSGKALEDPSVLPRFLVISFADLKKWSFRYWFAFPAFVLDPPVSLI-ELKPASEYFSS 179
Query: 170 KQLETLSQCYKSLEENQKN-----FFVVIKKNDDLSVKKLSEVFDVNSANCIDLDLVSTY 224
++ E++S ++ F V + + S++ L ++ D
Sbjct: 180 EEAESVSAACNDWRDSDLTTDVPFFLVSVSSDSKASIRHLKDL------EACQGDHQKLL 233
Query: 225 FVFADPSN-GCNPGWPLRTFLAALLEYCPELAKSTLQVIGLRSSMN-GDFIKSLV--FSI 280
F F DP + NPGWPLR +LA + T+ R S D SLV SI
Sbjct: 234 FGFYDPCHLPSNPGWPLRNYLALIRS---RWNLETVWFFCYRESRGFADLNLSLVGQASI 290
Query: 281 EIPQDIKPVESAGWVGWERNDKGNFGPRLANMSTSMDPVILADTSSDLNIKLMKWRLVPD 340
+ VGWE N KG PR +++ SMDP LA ++ DLN+KLM+WR +P
Sbjct: 291 TLSSGESAETVPNSVGWELN-KGKRVPRSISLANSMDPTRLAVSAVDLNLKLMRWRALPS 349
Query: 341 LNVGVMKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCL 400
LN+ V+ KCLLLGAGTLGC VAR L+ WG R+ITFVD GKV+ SNP RQ L+N++DCL
Sbjct: 350 LNLNVLSSVKCLLLGAGTLGCQVARTLMGWGIRNITFVDYGKVAMSNPVRQSLYNFEDCL 409
Query: 401 GGGRRKAEAAADNLKSILPTTNSKGIVAHIPMPGHPIGDSLKEETIGDIKRITEAISEHD 460
G G KA AA +LK I P + G+V IPMPGHPI ++ +GD KR++E I HD
Sbjct: 410 GRGEFKAVAAVKSLKQIFPAMETSGVVMAIPMPGHPISSQEEDSVLGDCKRLSELIESHD 469
Query: 461 VVFLLLDTREARWLPTLIAAQHRKIVINAALGFDSYLVMRHGI--STSSEEVGTLDKQYI 518
VFLL DTRE+RWLP+L+ A KI INAALGFDSY+VMRHG ++ S+++ LD
Sbjct: 470 AVFLLTDTRESRWLPSLLCANANKIAINAALGFDSYMVMRHGAGPTSLSDDMQNLDINKT 529
Query: 519 EGRYLGCYFCNDVTAPGNSLRDRTLDQQCTVTRPGVAAVAGALSVEILVALLQHPKRVDA 578
+ LGCYFCNDV AP +S+ DRTLDQQCTVTRPG+A +AGAL+VE+LV +LQHP ++A
Sbjct: 530 NTQRLGCYFCNDVVAPQDSMTDRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINA 589
Query: 579 PALYNFNKTEQEIPSQIEGVLGAVPHSIRGFLHSYQTIAPTCTKFKQCIACSDTVINKYR 638
N + + + LG +PH IRG + + I C ACS+TVI++YR
Sbjct: 590 K---GDNSSLSNTGNNDDSPLGILPHQIRGSVSQFSQITLLGQASNSCTACSETVISEYR 646
Query: 639 EEGLDFLLNVFNSGSYLEEVTGLSALHLSAEMSEILTLTDEED 681
E G F+L N +YLE++TGL+ L +A + D+ D
Sbjct: 647 ERGNSFILEAINHPTYLEDLTGLTELKKAANSFNLDWEDDDTD 689
>At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative
(ECR1) identical to putative ubiquitin activating enzyme
E1 [Arabidopsis thaliana] GI:2952433; similar to NEDD8
activating enzyme [Mus musculus] GI:17061821
Length = 454
Score = 52.8 bits (121), Expect = 7e-07
Identities = 30/91 (32%), Positives = 48/91 (52%), Gaps = 6/91 (6%)
Query: 339 PDLNVGVMKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQD 398
P L + + L++GAG LGC + ++L GFR++ +D ++ +N RQ LF +D
Sbjct: 37 PGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIED 96
Query: 399 CLGGGRRKAEAAADNLKSILPTTNSKGIVAH 429
G+ KAE AA K ++ + IV H
Sbjct: 97 V---GKPKAEVAA---KRVMERVSGVEIVPH 121
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 50.0 bits (114), Expect = 5e-06
Identities = 28/88 (31%), Positives = 42/88 (47%), Gaps = 3/88 (3%)
Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405
+K K L++GAG +GC + + L GF I +D + SN RQ LF G+
Sbjct: 10 IKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHV---GQS 66
Query: 406 KAEAAADNLKSILPTTNSKGIVAHIPMP 433
KA+ A D + P N + A++ P
Sbjct: 67 KAKVARDAVLRFRPNINIRSYHANVKNP 94
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 50.0 bits (114), Expect = 5e-06
Identities = 28/88 (31%), Positives = 42/88 (47%), Gaps = 3/88 (3%)
Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRR 405
+K K L++GAG +GC + + L GF I +D + SN RQ LF G+
Sbjct: 10 IKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHV---GQS 66
Query: 406 KAEAAADNLKSILPTTNSKGIVAHIPMP 433
KA+ A D + P N + A++ P
Sbjct: 67 KAKVARDAVLRFRPNINIRSYHANVKNP 94
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 35.5 bits (78), Expect = 0.12
Identities = 25/86 (29%), Positives = 39/86 (45%), Gaps = 8/86 (9%)
Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFR-----HITFVDNGKVSYSNPTRQVLFNYQDCL 400
++D K +G+G LGC +NL G +T D+ + SN +RQ LF +
Sbjct: 490 LEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNI- 548
Query: 401 GGGRRKAEAAADNLKSILPTTNSKGI 426
G+ K+ AA I P N + +
Sbjct: 549 --GQAKSTVAASAAAVINPRFNIEAL 572
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 34.7 bits (76), Expect = 0.20
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 5/54 (9%)
Query: 346 MKDTKCLLLGAGTLGCHVARNLLAWGFR-----HITFVDNGKVSYSNPTRQVLF 394
++D + ++GAG LGC +NL G +T D+ + SN +RQ LF
Sbjct: 487 LEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQFLF 540
>At1g36050.1 68414.m04479 expressed protein
Length = 386
Score = 32.3 bits (70), Expect = 1.1
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 2/84 (2%)
Query: 8 TEIIQYVPFSSFVHPSFWHTLTEMKLEVDKLKETTKQIFGRFTYRCDIGSVFEVDGTSFN 67
+ ++ ++ F S + + HT+TE KL VD + T +I T+ S+ VD +
Sbjct: 35 SSVVMFLLFFSELR-LYLHTVTETKLIVDTSRGETLRINFDITFPALACSILSVDAMDIS 93
Query: 68 KTPHLEQQYHHVMGTI-MNKNTIE 90
HL+ ++ + + N NTIE
Sbjct: 94 GELHLDVKHDIIKRRLDSNGNTIE 117
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 31.9 bits (69), Expect = 1.4
Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Query: 268 MNGDFIKSL--VFSIEIPQDIKPVESAGWVGWERN 300
+ GDF K V + E+P+ KPV+ AGW W N
Sbjct: 661 VEGDFEKDKQEVLNQEVPEPEKPVQVAGWGHWTNN 695
>At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3 /
molybdopterin synthase sulphurylase (CNX5) identical to
SP|Q9ZNW0 Molybdenum cofactor synthesis protein 3
(Molybdopterin synthase sulfurylase) (MPT synthase
sulfurylase) {Arabidopsis thaliana}; contains Pfam
profiles PF00899: ThiF family, PF00581: Rhodanese-like
domain, PF05237: MoeZ/MoeB domain
Length = 464
Score = 30.3 bits (65), Expect = 4.4
Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Query: 352 LLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKAEAAA 411
L++GAG LG L A G + +D+ V +N RQ++ + + +G + K+ AAA
Sbjct: 95 LVIGAGGLGSPALLYLAACGVGQLGIIDHDVVELNNMHRQII-HTEAFIGHPKVKSAAAA 153
>At5g19000.1 68418.m02257 speckle-type POZ protein-related contains
Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917:
MATH domain; similar to Speckle-type POZ protein
(SP:O43791) [Homo sapiens]
Length = 442
Score = 30.3 bits (65), Expect = 4.4
Identities = 30/104 (28%), Positives = 47/104 (45%), Gaps = 13/104 (12%)
Query: 53 CDIGSVFEVDGTSFN--------KTPHLEQQYHHVMGTIMNKN-TIEDFKSIDKASLLNS 103
CD+ VF+VDG +FN ++P Q +G K TIED ++ L +
Sbjct: 203 CDV--VFQVDGETFNAHKLVLATRSPVFNAQLFGPLGDRNTKCITIEDMEAPIFKVLPLT 260
Query: 104 IGEMIWSNLRERTWITNPSALLNFFILSFADLKKFHYYYWFAFP 147
+ +++S + ++P ALL F L D H+ YW P
Sbjct: 261 LLLIVYSRMYHPG--SSPGALLLFSSLLTRDKVLLHFIYWDELP 302
>At1g63390.1 68414.m07168 flavin-containing monooxygenase-related /
FMO-related low similarity to flavin-containing
monooxygenase (FMO3) from Mus musculus [SP|P97501]
Length = 125
Score = 30.3 bits (65), Expect = 4.4
Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 4/52 (7%)
Query: 357 GTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKAE 408
G+L ++ R + G+R FV VS S R+ F C GGGRRK E
Sbjct: 75 GSLRTNLPRECM--GYRDFPFVVRSGVSESRDPRR--FPSHGCSGGGRRKRE 122
>At1g51720.1 68414.m05828 glutamate dehydrogenase, putative similar
to NADP-specific glutatamate dehydrogenase (NADP-GDH)
SP:P28724 [Giardia lamblia (Giardia intestinalis)]
Length = 637
Score = 30.3 bits (65), Expect = 4.4
Identities = 21/80 (26%), Positives = 39/80 (48%), Gaps = 7/80 (8%)
Query: 302 KGNF-GPRLANMSTSMDPVILADTSSDLNIKLMKWRLVPDLNVGVMKDTKCLLLGAGTLG 360
+G+F GPR+ ++S L +S + ++ D+N + K +C++ G G +
Sbjct: 376 QGSFTGPRIYWAASS-----LRTEASGYGVVYFARLILADMNKEI-KGLRCVVSGCGKIA 429
Query: 361 CHVARNLLAWGFRHITFVDN 380
HV L+A G +T D+
Sbjct: 430 MHVVEKLIACGAHPVTVSDS 449
>At5g37530.1 68418.m04520 thiF family protein similar to SP|P30138
Adenylyltransferase thiF (EC 2.7.7.-) {Escherichia
coli}; contains Pfam profile PF00899: ThiF family
Length = 457
Score = 29.5 bits (63), Expect = 7.7
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 3/68 (4%)
Query: 352 LLLGAGTLGCHVARNLLAWGFRHITFVDNGKVSYSNPTRQVLFNYQDCLGGGRRKAEAAA 411
+++G G +G H A LL G + VD +VS S+ R + D G KA
Sbjct: 97 VVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSLNRHAVATRADV---GIPKAMCLK 153
Query: 412 DNLKSILP 419
+ SI P
Sbjct: 154 KHFSSIFP 161
>At4g39030.1 68417.m05528 enhanced disease susceptibility 5 (EDS5) /
salicylic acid induction deficient 1 (SID1) identical to
SP|Q945F0; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 543
Score = 29.5 bits (63), Expect = 7.7
Identities = 16/71 (22%), Positives = 36/71 (50%), Gaps = 5/71 (7%)
Query: 86 KNTIEDFKSIDKASLLNSIGEMIW-----SNLRERTWITNPSALLNFFILSFADLKKFHY 140
KN+ K++ A+++N +G+ I + W T S +++ +++ + K+ +
Sbjct: 248 KNSWGPLKALAAATIINGLGDTILCLFLGQGIAGAAWATTASQIVSAYMMMDSLNKEGYN 307
Query: 141 YYWFAFPAPSQ 151
Y FA P+P +
Sbjct: 308 AYSFAIPSPQE 318
>At2g18810.1 68415.m02189 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 234
Score = 29.5 bits (63), Expect = 7.7
Identities = 12/50 (24%), Positives = 28/50 (56%)
Query: 165 DYFNNKQLETLSQCYKSLEENQKNFFVVIKKNDDLSVKKLSEVFDVNSAN 214
D+ N ++LE + + YK + ++ K V++ ++ L K + +D+ S +
Sbjct: 94 DFLNEEELEKIDRHYKKISDSDKGADVILVNSEGLQRKLKLKRWDMTSTS 143
>At1g60140.1 68414.m06775 glycosyl transferase family 20 protein /
trehalose-phosphatase family protein contains Pfam
profile: PF02358 trehalose-phosphatase
Length = 861
Score = 29.5 bits (63), Expect = 7.7
Identities = 29/114 (25%), Positives = 48/114 (42%), Gaps = 9/114 (7%)
Query: 410 AADNLKSILPTTNSKGIVAHIPMPGHPIGDSLKE---ETIGDIKRITEAISEHDV-VFLL 465
A ++L P+ K ++ I P G ++E ET +KRI E HD +L
Sbjct: 355 AFEHLLQQYPSMLGKIVLIQIVNPARGSGKDVQEARKETYDTVKRINERYGSHDYEPVVL 414
Query: 466 LDTREARWLPTLIAAQHRKIVINAAL-GFD----SYLVMRHGISTSSEEVGTLD 514
+D R+ + A ++NA G + Y V R G + ++ +G D
Sbjct: 415 IDRPVPRFEKSAYYALAECCIVNAVRDGMNLVPYKYTVCRQGTPSMNKSLGVSD 468
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.135 0.408
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,246,027
Number of Sequences: 28952
Number of extensions: 700586
Number of successful extensions: 1689
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1675
Number of HSP's gapped (non-prelim): 19
length of query: 681
length of database: 12,070,560
effective HSP length: 86
effective length of query: 595
effective length of database: 9,580,688
effective search space: 5700509360
effective search space used: 5700509360
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 63 (29.5 bits)
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