BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001458-TA|BGIBMGA001458-PA|IPR002290|Serine/threonine protein kinase, IPR000719|Protein kinase, IPR008271|Serine/threonine protein kinase, active site, IPR011009|Protein kinase-like (403 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 91 2e-20 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 65 1e-12 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 63 4e-12 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 59 8e-11 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 58 2e-10 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 58 2e-10 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 56 7e-10 AB193550-1|BAD66824.1| 699|Apis mellifera soluble guanylyl cycl... 23 3.5 DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 23 6.1 AY395071-1|AAQ96727.1| 646|Apis mellifera GABA neurotransmitter... 23 6.1 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 90.6 bits (215), Expect = 2e-20 Identities = 60/225 (26%), Positives = 110/225 (48%), Gaps = 6/225 (2%) Query: 80 EKIGELSDDDFEKLGELGQGNGGVV--MKVRHKSTGLIMARKLIHLEVKPAIKKQ-IIRE 136 E+ +L D L LG G G V +++ S+ +++ ++ ++Q I+ E Sbjct: 357 EEFRDLRLQDLRPLATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIMSE 416 Query: 137 LKVLHECNFAHIVGFYGAFYSDGEISICMEYMDGGSLDLILKKAGRIPESILGTITSAVL 196 +++ E + +V + F + + ME GG L +L+ G + T+ V+ Sbjct: 417 KRIMGEADCDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVV 476 Query: 197 KGLSYLRDKHAIMHRDVKPSNILVNSNGEIKICDFGVSGQLIDSMAN-SFVGTRSYMSPE 255 + YL ++ I++RD+KP N+L++S G +K+ DFG + +L +F GT Y++PE Sbjct: 477 EAFDYLHSRN-IIYRDLKPENLLLDSQGYVKLVDFGFAKRLDHGRKTWTFCGTPEYVAPE 535 Query: 256 RLQGTHYSVQSDIWSLGLSLVEMAIGMYPIPPPD-AKTLAAIFGG 299 + + + +D WSLG+ + E+ G P D KT I G Sbjct: 536 VILNKGHDISADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILKG 580 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 64.9 bits (151), Expect = 1e-12 Identities = 50/199 (25%), Positives = 88/199 (44%), Gaps = 10/199 (5%) Query: 94 GELGQGNGGVVMKVRHKSTGLIMARKLIHLEVKPAIKKQIIRELKVLHECNFAHIVGFYG 153 GE G G + T + +A K + + + E ++ + +++ G Sbjct: 642 GEFGDVCRGKLKLPPDGRTEIDVAIKTLKPGSADKARNDFLTEASIMGQFEHPNVIFLQG 701 Query: 154 AFYSDGEISICMEYMDGGSLDLILK-KAGRIPESILGTITSAVLKGLSYLRDKHAIMHRD 212 + I E+M+ GSLD L+ G+ L + + G+ YL + + + HRD Sbjct: 702 VVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRGIASGMQYLAEMNYV-HRD 760 Query: 213 VKPSNILVNSNGEIKICDFGVSGQLIDSMANSFVGTRS------YMSPERLQGTHYSVQS 266 + N+LVN+ KI DFG+S + I+S TR + +PE + ++ S Sbjct: 761 LAARNVLVNAALVCKIADFGLSRE-IESATEGAYTTRGGKIPVRWTAPEAIAFRKFTSAS 819 Query: 267 DIWSLGLSLVE-MAIGMYP 284 D+WS+G+ E M+ G P Sbjct: 820 DVWSMGIVCWEVMSYGERP 838 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 63.3 bits (147), Expect = 4e-12 Identities = 59/191 (30%), Positives = 90/191 (47%), Gaps = 12/191 (6%) Query: 96 LGQGNGGVVMKVRHKSTGLIMARKLIHLEVKPAIKKQIIRELKVLHECNFAHIVGF-YGA 154 LG G G+V K +K G +A K+I E K + + L N ++ GA Sbjct: 73 LGSGGFGIVYKALYK--GEQVAAKIIQTE-KYSNMLNSEKHASFLKHSNIVKVLMIEQGA 129 Query: 155 FYSDGEISICMEYMDGGSLDLILKKAGRIPESILGTITSAVLKGLSYLRDKHAIMHRDVK 214 S + +C + + IL K RI IL +IT A L + + I+H DVK Sbjct: 130 SLSLITMELCGTTLQNRLDEAILIKNERI--CILKSITCA----LQFCHNA-GIVHADVK 182 Query: 215 PSNILVNSNGEIKICDFGVSGQL-IDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSLGL 273 P NIL++ NG+ K+ DFG S + + + F GT Y +PE ++ + +DI+SLG+ Sbjct: 183 PKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAPEVIKQNRPTPAADIYSLGI 242 Query: 274 SLVEMAIGMYP 284 +M P Sbjct: 243 VAWQMLFRKLP 253 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 58.8 bits (136), Expect = 8e-11 Identities = 32/103 (31%), Positives = 56/103 (54%), Gaps = 3/103 (2%) Query: 148 IVGFYGAFYSDGEISICMEYMDGGSLDLILKKAGRIPESILGTITSAVLKGLSYLRDKHA 207 +V + F + + MEY++GG L +++ G+ E + S + GL +L + Sbjct: 47 LVQLHSCFQTMDRLYFVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGR-G 105 Query: 208 IMHRDVKPSNILVNSNGEIKICDFGVSGQLI--DSMANSFVGT 248 I++RD+K N+L++ +G IKI DFG+ + I D +F GT Sbjct: 106 IVYRDLKLDNVLLDQDGHIKIADFGMCKEGISGDKTTKTFCGT 148 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 57.6 bits (133), Expect = 2e-10 Identities = 32/92 (34%), Positives = 50/92 (54%), Gaps = 3/92 (3%) Query: 195 VLKGLSYLRDKHAIMHRDVKPSNILVNSNGEIKICDFGVSGQLIDSMANSFVGTRSYMSP 254 VL+G+ YL + ++HRDVK N+L++ K+ DFG + M S VGT +M+P Sbjct: 706 VLEGIRYLHSQ-GLVHRDVKLKNVLLDIENRAKLTDFGFCITEV-MMLGSIVGTPVHMAP 763 Query: 255 ERLQGTHYSVQSDIWSLGLSLVEMAIGMYPIP 286 E L G HY D+++ G+ + G +P Sbjct: 764 ELLSG-HYDSSVDVYAFGILFWYLCAGHVRLP 794 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 57.6 bits (133), Expect = 2e-10 Identities = 32/92 (34%), Positives = 50/92 (54%), Gaps = 3/92 (3%) Query: 195 VLKGLSYLRDKHAIMHRDVKPSNILVNSNGEIKICDFGVSGQLIDSMANSFVGTRSYMSP 254 VL+G+ YL + ++HRDVK N+L++ K+ DFG + M S VGT +M+P Sbjct: 744 VLEGIRYLHSQ-GLVHRDVKLKNVLLDIENRAKLTDFGFCITEV-MMLGSIVGTPVHMAP 801 Query: 255 ERLQGTHYSVQSDIWSLGLSLVEMAIGMYPIP 286 E L G HY D+++ G+ + G +P Sbjct: 802 ELLSG-HYDSSVDVYAFGILFWYLCAGHVRLP 832 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 55.6 bits (128), Expect = 7e-10 Identities = 35/107 (32%), Positives = 55/107 (51%), Gaps = 6/107 (5%) Query: 206 HAIMHRDVKPSNILVNSNGE---IKICDFGVSGQLIDSMAN--SFVGTRSYMSPERLQGT 260 + ++HRD+KP N+L+ S + +K+ DFG++ ++ F GT Y+SPE L+ Sbjct: 28 NGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEAQAWFGFAGTPGYLSPEVLKKE 87 Query: 261 HYSVQSDIWSLGLSLVEMAIGMYPIPPPDAKTL-AAIFGGQNEDHSP 306 Y DIW+ G+ L + +G P D L A I G + SP Sbjct: 88 PYGKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIKTGSYDYPSP 134 >AB193550-1|BAD66824.1| 699|Apis mellifera soluble guanylyl cyclase alpha 1 subunit protein. Length = 699 Score = 23.4 bits (48), Expect = 3.5 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%) Query: 87 DDDFEKLGELGQGNGGVVMKVRHKSTGLIMARKLIHLEVKPAIK-KQIIRELKVLHECNF 145 D+D ++ LG+ +GG + + T L+M + E + K + + VL + Sbjct: 22 DNDRSRIARLGRDDGGKSRQSSFEVTSLLMREETEDAEDTQTLNLKHLRAAVLVLTNPSN 81 Query: 146 AHIVGFYGAFYSDGEIS 162 + GA S GE S Sbjct: 82 EVVAVALGALLSKGEES 98 >DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine receptor alpha1subunit protein. Length = 601 Score = 22.6 bits (46), Expect = 6.1 Identities = 8/26 (30%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Query: 121 IHLEVKPAIKKQIIREL-KVLHECNF 145 +H +V P +K ++RE+ K + + F Sbjct: 496 VHEDVSPTFEKPLVREIEKTIDDARF 521 >AY395071-1|AAQ96727.1| 646|Apis mellifera GABA neurotransmitter transporter-1B protein. Length = 646 Score = 22.6 bits (46), Expect = 6.1 Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 3/66 (4%) Query: 309 APNSPRPMAIFELLDYIVNEPPPKLPSGIFSDEFKDFVDRCLKKNPDERADLKTLMNH-E 367 +P +P+ + L + EPP ++ +G +F+ +CL+ + + ++KTL + Sbjct: 17 SPLPRKPVNPVQELKALFAEPPVRVSNGGKEQDFRFEAIQCLQSSAFK--EIKTLPDRGM 74 Query: 368 WIRKAE 373 W K E Sbjct: 75 WSSKIE 80 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.316 0.134 0.391 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 119,660 Number of Sequences: 429 Number of extensions: 5338 Number of successful extensions: 23 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 6 Number of HSP's gapped (non-prelim): 11 length of query: 403 length of database: 140,377 effective HSP length: 59 effective length of query: 344 effective length of database: 115,066 effective search space: 39582704 effective search space used: 39582704 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 45 (22.2 bits)
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