Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001450-TA|BGIBMGA001450-PA|IPR004182|GRAM
(374 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g02120.1 68414.m00138 GRAM domain-containing protein-related ... 73 2e-13
At3g59660.1 68416.m06656 C2 domain-containing protein / GRAM dom... 47 2e-05
At5g50170.1 68418.m06213 C2 domain-containing protein / GRAM dom... 44 1e-04
At1g03370.1 68414.m00316 C2 domain-containing protein / GRAM dom... 40 0.002
At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containi... 30 2.9
At5g23630.1 68418.m02771 ATPase E1-E2 type family protein / halo... 30 2.9
At5g32169.1 68418.m03692 hypothetical protein 29 5.0
At5g27650.1 68418.m03313 PWWP domain-containing protein hypothet... 29 5.0
At2g23360.1 68415.m02790 transport protein-related contains Pfam... 29 5.0
At5g55920.1 68418.m06975 nucleolar protein, putative similar to ... 28 8.8
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 28 8.8
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 28 8.8
At2g22475.2 68415.m02665 GRAM domain-containing protein / ABA-re... 28 8.8
At2g22475.1 68415.m02666 GRAM domain-containing protein / ABA-re... 28 8.8
>At1g02120.1 68414.m00138 GRAM domain-containing protein-related
contains low similarity to PF02893: GRAM domain
Length = 556
Score = 73.3 bits (172), Expect = 2e-13
Identities = 30/75 (40%), Positives = 46/75 (61%)
Query: 86 QGHLYITKNYFAFYSNVFGYVTKLLIPTTSVLRITKEKVARIIPNAVGVCTRDERHVFGS 145
+GH+Y+ +Y FYSN+FGY TK +IP + + + K A I PNA+ + +++ F S
Sbjct: 128 EGHMYLFIHYICFYSNIFGYETKKIIPFAEISCVKRAKTAGIFPNAIEILAGGKKYFFAS 187
Query: 146 LLSRDSTYKLMMHVW 160
LSRD +KL+ W
Sbjct: 188 FLSRDEAFKLIHDGW 202
>At3g59660.1 68416.m06656 C2 domain-containing protein / GRAM
domain-containing protein low similarity to GLUT4
vesicle protein [Rattus norvegicus] GI:4193489; contains
Pfam profiles PF00168: C2 domain, PF02893: GRAM domain
Length = 594
Score = 47.2 bits (107), Expect = 2e-05
Identities = 29/88 (32%), Positives = 45/88 (51%), Gaps = 3/88 (3%)
Query: 44 TKDSKQPSKARQKK--FQRHFPQVGPEEKVLNYYSCALVGDLLLQGHLYITKNYFAFYSN 101
T D + P+ QK Q F + P+E V + YSCAL L G +Y++ + F+SN
Sbjct: 215 TLDQQGPTIVHQKPGPLQTIFDLL-PDEVVEHSYSCALERSFLYHGRMYVSAWHICFHSN 273
Query: 102 VFGYVTKLLIPTTSVLRITKEKVARIIP 129
VF K+++P + I + + A I P
Sbjct: 274 VFSKQMKVVVPLGDIDEIRRSQHALINP 301
>At5g50170.1 68418.m06213 C2 domain-containing protein / GRAM
domain-containing protein low similarity to SP|P40748
Synaptotagmin III (SytIII) {Rattus norvegicus}; contains
Pfam profiles PF00168: C2 domain, PF02893: GRAM domain
Length = 1027
Score = 44.4 bits (100), Expect = 1e-04
Identities = 41/132 (31%), Positives = 63/132 (47%), Gaps = 21/132 (15%)
Query: 51 SKARQKKFQRHFPQVGPEEKVLNYYSCALVGDLLLQGHLYITKNYFAFYSNVFGYVTKL- 109
S + FQ+ F + EE +L Y+C L L +QG L+++ AFYSNVFG+ TK
Sbjct: 687 SPQKNSAFQKLFG-LPHEEFLLKEYTCYLKRKLPVQGKLFLSARIVAFYSNVFGHKTKFY 745
Query: 110 ----------LIPTT-----SVLRITKEKVARIIPNAVGVCTRDER----HVFGSLLSRD 150
++P T S L + K R + G ++D+ F S +S D
Sbjct: 746 FLWEDIDDIQVLPPTFASLGSPLLLIILKKNRGLDAKHGAKSQDDEGRLSFYFQSFVSFD 805
Query: 151 STYKLMMHVWKT 162
+T + +M +WKT
Sbjct: 806 ATSRTIMALWKT 817
>At1g03370.1 68414.m00316 C2 domain-containing protein / GRAM
domain-containing protein contains Pfam profiles PF00168:
C2 domain; contains PF02893: GRAM domain; similar to
Chain A, Crystal Structure Of Synaptotagmin Iii C2aC2B
Length(GI:6980525); similar to Synaptotagmin III (SytIII)
(Swiss-Prot:P40748) [Rattus norvegicus]
Length = 1859
Score = 40.3 bits (90), Expect = 0.002
Identities = 43/145 (29%), Positives = 66/145 (45%), Gaps = 26/145 (17%)
Query: 47 SKQPSKARQKKFQRHFPQVGPEEKVLNYYSCALVGDLLLQGHLYITKNYFAFYSNVFGYV 106
S Q + A QK F PQ EE ++N ++C L + LQG L+++ FY+++FG
Sbjct: 1522 SPQTNSAFQKLFG--LPQ---EEFLINDFTCHLKRKMPLQGRLFLSARIVGFYASIFGNK 1576
Query: 107 TKL-----------LIPTTSVLRITKEKVARIIPN-----AVGVCTRDE----RHVFGSL 146
TK ++P T + V + PN +G T DE + F S
Sbjct: 1577 TKFFFLWEDIEEIQVLPPTLASMGSPIVVMTLRPNRGLDARIGAKTHDEEGRLKFHFHSF 1636
Query: 147 LSRDSTYKLMMHVWKT-ARTPELAV 170
+S + K +M +WK + TPE V
Sbjct: 1637 VSFNVAQKTIMALWKAKSLTPEQKV 1661
>At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 723
Score = 29.9 bits (64), Expect = 2.9
Identities = 25/82 (30%), Positives = 35/82 (42%), Gaps = 7/82 (8%)
Query: 184 PQLVVSSFHDTL--LLKDPRASEVDFEASEYSPEDDSSSAGGDQVDAPAAVRRESETILP 241
P + +S F D L ++ P S ++ E SE EDD GD P +S T+
Sbjct: 55 PSIFLSCFDDALPDKIQQPENSTINSEESECEEEDDEE---GDDFTDPILKFFKSRTL-- 109
Query: 242 TGAAVIQPALLTGSSTPKRRAT 263
T + PA + S K R T
Sbjct: 110 TSESTADPARESKFSLQKNRRT 131
>At5g23630.1 68418.m02771 ATPase E1-E2 type family protein /
haloacid dehalogenase-like hydrolase familiy protein
similar to SP|O14072 Cation-transporting ATPase 4 (EC
3.6.3.-) {Schizosaccharomyces pombe}; contains InterPro
accession IPR001757: ATPase, E1-E2 type; contains Pfam
profile PF00702: haloacid dehalogenase-like hydrolase
Length = 1179
Score = 29.9 bits (64), Expect = 2.9
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 6/42 (14%)
Query: 218 SSSAGGDQVDAPAAVRRESETILPTGAAVIQPALLTGSSTPK 259
S+ GG+ PA + +L G+A++ A+LTG STP+
Sbjct: 285 STQTGGEDKTVPA------DMLLLVGSAIVNEAILTGESTPQ 320
>At5g32169.1 68418.m03692 hypothetical protein
Length = 258
Score = 29.1 bits (62), Expect = 5.0
Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 2/76 (2%)
Query: 44 TKDSKQPSKARQKKFQRHFPQVGPEEKVLNYYSCALVGDLLLQGHLYITKNYFAFYSNVF 103
T+ S K R + F R + VLN +V + + G+L I+ YF+F
Sbjct: 135 TRHSSTRKKRRLQLFTRPLTRPPGSSTVLNPSQYFVVLSIRVSGYLAISSMYFSFSQEEV 194
Query: 104 GYVTKLLIPTTSVLRI 119
Y+ +L P T R+
Sbjct: 195 SYL--VLFPQTLCSRL 208
>At5g27650.1 68418.m03313 PWWP domain-containing protein
hypothetical protein F22F7.12 - Arabidopsis thaliana,
EMBL:AC009606
Length = 1072
Score = 29.1 bits (62), Expect = 5.0
Identities = 15/35 (42%), Positives = 18/35 (51%)
Query: 193 DTLLLKDPRASEVDFEASEYSPEDDSSSAGGDQVD 227
D L+ KD +SEV E E DD SS G + D
Sbjct: 91 DVLIDKDDESSEVKEEEEEEDGSDDQSSELGSEAD 125
>At2g23360.1 68415.m02790 transport protein-related contains Pfam
PF05911: Plant protein of unknown function (DUF869)
profile; weak similarity to Intracellular protein
transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]
Length = 886
Score = 29.1 bits (62), Expect = 5.0
Identities = 16/41 (39%), Positives = 20/41 (48%)
Query: 122 EKVARIIPNAVGVCTRDERHVFGSLLSRDSTYKLMMHVWKT 162
+ + RII GV +DERHV R S Y + WKT
Sbjct: 538 KSIHRIIDVIEGVSLKDERHVSNRESERLSGYTARVLQWKT 578
>At5g55920.1 68418.m06975 nucleolar protein, putative similar to
SP|P46087 Proliferating-cell nucleolar antigen p120
(Proliferation-associated nucleolar protein p120) {Homo
sapiens}, SP|P40991 Nucleolar protein NOP2
{Saccharomyces cerevisiae}; contains Pfam profile
PF01189: NOL1/NOP2/sun family
Length = 682
Score = 28.3 bits (60), Expect = 8.8
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 206 DFEASEYSPEDDSSSAGGDQVDAPAAVRRE 235
D+E ++ S EDD S G D+ D AV E
Sbjct: 65 DYEVTDDSDEDDEVSDGSDEDDISPAVESE 94
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 28.3 bits (60), Expect = 8.8
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Query: 95 YFAFYSNVFGYVTKLLIPTTSVLRITKEKVARIIPNAVGVCTRDERHVFGSLLSRDSTYK 154
Y A+Y V +L V+ I KE V + +G +D +H FGS+L DS K
Sbjct: 376 YHAYYRKVLQEAEELKSGDKGVI-IRKEDVKQ---EKMGNAVKDSKHGFGSVLPDDSAKK 431
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 28.3 bits (60), Expect = 8.8
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Query: 95 YFAFYSNVFGYVTKLLIPTTSVLRITKEKVARIIPNAVGVCTRDERHVFGSLLSRDSTYK 154
Y A+Y V +L V+ I KE V + +G +D +H FGS+L DS K
Sbjct: 376 YHAYYRKVLQEAEELKSGDKGVI-IRKEDVKQ---EKMGNAVKDSKHGFGSVLPDDSAKK 431
>At2g22475.2 68415.m02665 GRAM domain-containing protein /
ABA-responsive protein-related similar to ABA-responsive
protein [Hordeum vulgare] GI:4103635; contains Pfam
profile PF02893: GRAM domain
Length = 248
Score = 28.3 bits (60), Expect = 8.8
Identities = 21/77 (27%), Positives = 40/77 (51%), Gaps = 6/77 (7%)
Query: 26 SAPSVRVSALGSVSGNDETKDSKQPSKARQKKFQRHFPQVGPEEKVLNYYSCALVGDL-L 84
+APS +A+G ++ + +K ++ +K R + PEE++LN ++C L
Sbjct: 149 TAPSFADAAMGRIA-----QSTKVFAEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAGP 203
Query: 85 LQGHLYITKNYFAFYSN 101
+ G LYI+ A+ S+
Sbjct: 204 VMGVLYISSAKLAYCSD 220
>At2g22475.1 68415.m02666 GRAM domain-containing protein /
ABA-responsive protein-related similar to ABA-responsive
protein [Hordeum vulgare] GI:4103635; contains Pfam
profile PF02893: GRAM domain
Length = 299
Score = 28.3 bits (60), Expect = 8.8
Identities = 21/77 (27%), Positives = 40/77 (51%), Gaps = 6/77 (7%)
Query: 26 SAPSVRVSALGSVSGNDETKDSKQPSKARQKKFQRHFPQVGPEEKVLNYYSCALVGDL-L 84
+APS +A+G ++ + +K ++ +K R + PEE++LN ++C L
Sbjct: 149 TAPSFADAAMGRIA-----QSTKVFAEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAGP 203
Query: 85 LQGHLYITKNYFAFYSN 101
+ G LYI+ A+ S+
Sbjct: 204 VMGVLYISSAKLAYCSD 220
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.132 0.389
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,013,302
Number of Sequences: 28952
Number of extensions: 299652
Number of successful extensions: 814
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 806
Number of HSP's gapped (non-prelim): 15
length of query: 374
length of database: 12,070,560
effective HSP length: 82
effective length of query: 292
effective length of database: 9,696,496
effective search space: 2831376832
effective search space used: 2831376832
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 60 (28.3 bits)
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