Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001437-TA|BGIBMGA001437-PA|IPR002735|Translation
initiation factor IF2/IF5
(310 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20920.2 68418.m02485 eukaryotic translation initiation facto... 215 2e-56
At5g20920.1 68418.m02484 eukaryotic translation initiation facto... 215 2e-56
At3g07920.1 68416.m00967 eukaryotic translation initiation facto... 134 5e-32
At5g01940.1 68418.m00113 eukaryotic translation initiation facto... 110 1e-24
At1g36730.1 68414.m04569 eukaryotic translation initiation facto... 61 1e-09
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 53 2e-07
At3g14980.1 68416.m01894 PHD finger transcription factor, putati... 29 3.0
At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger) fa... 29 5.2
At2g43710.2 68415.m05434 acyl-[acyl-carrier-protein] desaturase ... 29 5.2
At2g43710.1 68415.m05433 acyl-[acyl-carrier-protein] desaturase ... 29 5.2
At5g53020.1 68418.m06585 expressed protein 28 6.9
At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong simil... 28 6.9
At1g63210.1 68414.m07144 hypothetical protein contains Pfam prof... 28 6.9
At4g28365.1 68417.m04060 plastocyanin-like domain-containing pro... 28 9.2
At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,... 28 9.2
>At5g20920.2 68418.m02485 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 267
Score = 215 bits (526), Expect = 2e-56
Identities = 98/180 (54%), Positives = 132/180 (73%), Gaps = 5/180 (2%)
Query: 127 EDVRGEDQENVE----DEHGEWVGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFIMR 182
+++ ++QE E + W GS+ DY YDELL RVF+I+RE NP + +G +++ +MR
Sbjct: 87 DEIANDEQEGEEGIVLQQRYPWEGSERDYIYDELLGRVFNILRENNPEL-AGDRRRTVMR 145
Query: 183 PPQVVRIGTKKTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRFQQK 242
PPQV+R GTKKT F NF ++CKT+HRQ H++ +LLAELGTSGS+DG +L++KGRF K
Sbjct: 146 PPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRLVVKGRFAPK 205
Query: 243 QIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQCETCGSRCSVASIKSGFQAVTGKR 302
E +LRRYI +YV C C+SPDTIL K+ RLFFL+CE CGS+ SVA IK+GF A +R
Sbjct: 206 NFEGILRRYITDYVICLGCKSPDTILSKENRLFFLRCEKCGSQRSVAPIKTGFVARVSRR 265
>At5g20920.1 68418.m02484 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 268
Score = 215 bits (526), Expect = 2e-56
Identities = 98/180 (54%), Positives = 132/180 (73%), Gaps = 5/180 (2%)
Query: 127 EDVRGEDQENVE----DEHGEWVGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFIMR 182
+++ ++QE E + W GS+ DY YDELL RVF+I+RE NP + +G +++ +MR
Sbjct: 88 DEIANDEQEGEEGIVLQQRYPWEGSERDYIYDELLGRVFNILRENNPEL-AGDRRRTVMR 146
Query: 183 PPQVVRIGTKKTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRFQQK 242
PPQV+R GTKKT F NF ++CKT+HRQ H++ +LLAELGTSGS+DG +L++KGRF K
Sbjct: 147 PPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRLVVKGRFAPK 206
Query: 243 QIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQCETCGSRCSVASIKSGFQAVTGKR 302
E +LRRYI +YV C C+SPDTIL K+ RLFFL+CE CGS+ SVA IK+GF A +R
Sbjct: 207 NFEGILRRYITDYVICLGCKSPDTILSKENRLFFLRCEKCGSQRSVAPIKTGFVARVSRR 266
>At3g07920.1 68416.m00967 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 169
Score = 134 bits (325), Expect = 5e-32
Identities = 65/129 (50%), Positives = 89/129 (68%), Gaps = 5/129 (3%)
Query: 155 ELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRI----GTKKTSFANFTEICKTLHRQA 210
E+L RVF+I+RE +P +V G I+ PPQV+R GTKKT F NF + CKT+HR
Sbjct: 18 EILRRVFNILRENSPELV-GIWLLTIIWPPQVLREETAKGTKKTVFVNFMDYCKTMHRNP 76
Query: 211 KHLLDFLLAELGTSGSVDGNSQLIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQK 270
H++ FLLAELGT G +D +L+++GRF Q+ E++LR YI +YV C C+S + IL K
Sbjct: 77 DHVMAFLLAELGTRGKLDDQQRLVVRGRFTQRDFESLLRGYILDYVMCIPCKSTEAILSK 136
Query: 271 DTRLFFLQC 279
+ RLFFL+C
Sbjct: 137 ENRLFFLRC 145
>At5g01940.1 68418.m00113 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 231
Score = 110 bits (264), Expect = 1e-24
Identities = 58/145 (40%), Positives = 88/145 (60%), Gaps = 3/145 (2%)
Query: 150 DYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQ 209
DY Y ELL VFD +RE++ VS ++ + +M PPQ++ GT T NF ++C+T+HR+
Sbjct: 72 DYGYKELLSMVFDRLREEDVE-VSTERPRTVMMPPQLLAEGTI-TVCLNFADLCRTMHRK 129
Query: 210 AKHLLDFLLAELGTSGSVDGNSQLIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTIL- 268
H++ FLLA++ T S++ +L IKG K + V R+YI +V C C+SPDT L
Sbjct: 130 PDHVMKFLLAQMETKVSLNKQQRLEIKGLVSSKDFQAVFRKYIDAFVICVCCKSPDTALA 189
Query: 269 QKDTRLFFLQCETCGSRCSVASIKS 293
++ LF L+CET + S +S
Sbjct: 190 EEGNGLFNLRCETGKDEDKLVSFES 214
>At1g36730.1 68414.m04569 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 439
Score = 60.9 bits (141), Expect = 1e-09
Identities = 31/93 (33%), Positives = 47/93 (50%), Gaps = 2/93 (2%)
Query: 193 KTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDG-NSQLIIKGRFQQKQIENVLRRY 251
KT+ N EI K L R A + + ELG D N ++ G ++ +L +
Sbjct: 35 KTNVVNMVEIAKALGRPAAYTTKYFGCELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENF 94
Query: 252 IKEYVTCHTCRSPDT-ILQKDTRLFFLQCETCG 283
IK+YV C+ C +P+T IL T++ L+C CG
Sbjct: 95 IKKYVQCYGCGNPETEILITKTQMLQLKCAACG 127
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 53.2 bits (122), Expect = 2e-07
Identities = 27/93 (29%), Positives = 46/93 (49%), Gaps = 2/93 (2%)
Query: 193 KTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGNSQL-IIKGRFQQKQIENVLRRY 251
KT+ N EI K L R + + ELG D + ++ G ++ +L +
Sbjct: 35 KTNIINNVEIAKALARPPSYTTKYFGCELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENF 94
Query: 252 IKEYVTCHTCRSPDT-ILQKDTRLFFLQCETCG 283
IK++V C+ C +P+T I+ T++ L+C CG
Sbjct: 95 IKKFVQCYGCGNPETEIIITKTQMVNLKCAACG 127
>At3g14980.1 68416.m01894 PHD finger transcription factor, putative
contains Pfam profile: PF00628 PHD-finger
Length = 1189
Score = 29.5 bits (63), Expect = 3.0
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Query: 204 KTLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRFQQKQIENV 247
K LHR++K + D A L S+D NS + FQ K++ N+
Sbjct: 475 KNLHRRSKKISDIKPASLDQHDSLDSNS--LNSFEFQDKEMGNI 516
>At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger)
family protein contains similarity to polycomb-M33
interacting protein Ring1B [Mus musculus] GI:2239142;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 486
Score = 28.7 bits (61), Expect = 5.2
Identities = 14/54 (25%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Query: 127 EDVRGEDQENVEDEHGEWVGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFI 180
+D +++E VE E E + + +E E + +E++PS +SG + +F+
Sbjct: 72 DDAEEDEEEEVEAEEDEEAEEEEEEEEEE--EEEEEDSKERSPSSISGDQSEFM 123
>At2g43710.2 68415.m05434 acyl-[acyl-carrier-protein] desaturase /
stearoyl-ACP desaturase (SSI2) identical to gi:15149310;
contains Pfam profile PF03405: Fatty acid desaturase;
identical to cDNA stearoyl ACP desaturase (SSI2),
SSI2-FAB2 allele, GI:15149309
Length = 401
Score = 28.7 bits (61), Expect = 5.2
Identities = 13/58 (22%), Positives = 29/58 (50%)
Query: 149 HDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTL 206
H+ +Y +++E++F+I + + +K I P ++ G F NF+ + + L
Sbjct: 270 HETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFSSVAQRL 327
>At2g43710.1 68415.m05433 acyl-[acyl-carrier-protein] desaturase /
stearoyl-ACP desaturase (SSI2) identical to gi:15149310;
contains Pfam profile PF03405: Fatty acid desaturase;
identical to cDNA stearoyl ACP desaturase (SSI2),
SSI2-FAB2 allele, GI:15149309
Length = 401
Score = 28.7 bits (61), Expect = 5.2
Identities = 13/58 (22%), Positives = 29/58 (50%)
Query: 149 HDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTL 206
H+ +Y +++E++F+I + + +K I P ++ G F NF+ + + L
Sbjct: 270 HETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFSSVAQRL 327
>At5g53020.1 68418.m06585 expressed protein
Length = 721
Score = 28.3 bits (60), Expect = 6.9
Identities = 33/126 (26%), Positives = 57/126 (45%), Gaps = 15/126 (11%)
Query: 140 EHGEWVGSDHD---YSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKT-S 195
EH +W D D YS +L E D +K ++V G+++ I IG K+
Sbjct: 289 EHLDW-NKDPDVVPYSIGDLSELGVDGNAKKRENLVFGEEELCIRV------IGNKQEIE 341
Query: 196 FANFTEICKTLHRQAK----HLLDFLLAELGTSGSVDGNSQLIIKGRFQQKQIENVLRRY 251
+FTE K + + HL++ L ++G SQ +++ R Q+E ++ R
Sbjct: 342 IGDFTEHMKLKDEKVETLCLHLMNSELESKRLRSCIEGLSQEMLQLRHDNTQLEGMVNRR 401
Query: 252 IKEYVT 257
+E V+
Sbjct: 402 GEESVS 407
>At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong
similarity to acyl-CoA oxidase [Arabidopsis thaliana]
GI:3044214
Length = 664
Score = 28.3 bits (60), Expect = 6.9
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Query: 128 DVRGEDQENVEDEHGEWVGSDHDYSYDELLERVF--DIMREKNPSMVSGKKQKF 179
D R + + NV+D W GS H + + R+ D + EK+ V +K+ F
Sbjct: 9 DERNKAEFNVDDMKIVWAGSRHAFDVSNRMSRLVANDPVFEKSKRAVMSRKELF 62
>At1g63210.1 68414.m07144 hypothetical protein contains Pfam profile:
PF04283 protein of unknown function (DUF439)
Length = 1197
Score = 28.3 bits (60), Expect = 6.9
Identities = 26/105 (24%), Positives = 47/105 (44%), Gaps = 2/105 (1%)
Query: 145 VGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICK 204
+G DY ++E V I +EK P K + I+ P + T + + I +
Sbjct: 973 LGDVDDYYHEEKRNSVL-IEKEKVPKEKKQFKSRMIVHPRFQNITAEQATVYLSDKNIGE 1031
Query: 205 TLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRFQQKQIENVLR 249
++ R + L+ L + S +V N + II+G + K I ++ R
Sbjct: 1032 SIVRPSSRGLNHLTLMIKISDNVYANKE-IIEGEKENKDIVSLQR 1075
>At4g28365.1 68417.m04060 plastocyanin-like domain-containing
protein
Length = 199
Score = 27.9 bits (59), Expect = 9.2
Identities = 19/55 (34%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Query: 216 FLLAELGTSGSVDGNSQLIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQK 270
FLL+ G+ + GNSQ +KG QK VL + HT SP + Q+
Sbjct: 98 FLLSHSGSYFFISGNSQNCLKG---QKLAVKVLSTVHHSHSPRHTSPSPSPVHQE 149
>At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,
putative / stearoyl-ACP desaturase, putative similar to
Acyl-[acyl-carrier protein] desaturase from Spinacia
oleracea SP|P28645, Olea europaea SP|Q43593; contains
Pfam profile PF03405 Fatty acid desaturase
Length = 396
Score = 27.9 bits (59), Expect = 9.2
Identities = 11/58 (18%), Positives = 29/58 (50%)
Query: 149 HDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTL 206
H+ +Y ++E++F+I + ++ +K I P Q++ G F ++ + + +
Sbjct: 260 HEMAYTRIVEKLFEIDPDGTVQALASMMRKRITMPAQLMHDGRDDNLFDHYAAVAQRI 317
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.134 0.390
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,106,168
Number of Sequences: 28952
Number of extensions: 228279
Number of successful extensions: 820
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 799
Number of HSP's gapped (non-prelim): 20
length of query: 310
length of database: 12,070,560
effective HSP length: 81
effective length of query: 229
effective length of database: 9,725,448
effective search space: 2227127592
effective search space used: 2227127592
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 59 (27.9 bits)
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