BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001425-TA|BGIBMGA001425-PA|IPR001739|Methyl-CpG binding (249 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g59380.1 68418.m07441 methyl-CpG-binding domain-containing pr... 56 2e-08 At3g46580.1 68416.m05056 methyl-CpG-binding domain-containing pr... 52 5e-07 At5g35330.2 68418.m04188 methyl-CpG-binding domain-containing pr... 45 6e-05 At5g35330.1 68418.m04187 methyl-CpG-binding domain-containing pr... 45 6e-05 At3g63030.1 68416.m07080 methyl-CpG-binding domain-containing pr... 43 2e-04 At4g22745.1 68417.m03282 methyl-CpG-binding domain-containing pr... 40 0.002 At5g59800.1 68418.m07497 methyl-CpG-binding domain-containing pr... 40 0.002 At5g35338.2 68418.m04194 methyl-CpG-binding domain-containing pr... 36 0.026 At4g39680.1 68417.m05614 SAP domain-containing protein contains ... 34 0.079 At5g08230.1 68418.m00965 PWWP domain-containing protein putative... 30 1.3 At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing pr... 30 1.3 At3g15790.1 68416.m01999 methyl-CpG-binding domain-containing pr... 30 1.7 At5g47580.1 68418.m05873 expressed protein strong similarity to ... 29 2.2 At3g01460.1 68416.m00070 PHD finger family protein / methyl-CpG ... 29 3.0 At1g70070.1 68414.m08062 DEAD/DEAH box helicase, putative simila... 29 3.9 At3g28030.1 68416.m03499 UV hypersensitive protein (UVH3) / DNA-... 28 5.2 At3g14980.1 68416.m01894 PHD finger transcription factor, putati... 28 5.2 At1g15340.1 68414.m01835 methyl-CpG-binding domain-containing pr... 28 5.2 >At5g59380.1 68418.m07441 methyl-CpG-binding domain-containing protein similar to testis specific methyl-CpG binding protein MBD2 [Homo sapiens] GI:3800801; contains Pfam profile PF01429: Methyl-CpG binding domain Length = 225 Score = 56.0 bits (129), Expect = 2e-08 Identities = 24/44 (54%), Positives = 33/44 (75%), Gaps = 1/44 (2%) Query: 15 LPKGWQREEVVRKTGLSAGKVDVYYYSP-TGKKFRSKPELVRYL 57 LP GW+ E+ +R +G +AG VD YYY P TG+KFRS+ E++ YL Sbjct: 81 LPPGWRVEDKIRTSGATAGSVDKYYYEPNTGRKFRSRTEVLYYL 124 >At3g46580.1 68416.m05056 methyl-CpG-binding domain-containing protein weak similarity to SP|P51608 Methyl-CpG-binding protein 2 (MeCP-2 protein) (MeCP2) {Homo sapiens}; contains Pfam profile PF01429: Methyl-CpG binding domain Length = 182 Score = 51.6 bits (118), Expect = 5e-07 Identities = 29/58 (50%), Positives = 37/58 (63%), Gaps = 4/58 (6%) Query: 4 SIERKRS---DCSALPKGWQREEVVRKTGLSAGKVDVYYYSP-TGKKFRSKPELVRYL 57 S RKR+ D + LP W+ E VR +G AG VD +YY P TG+KFRSK E++ YL Sbjct: 21 SKSRKRATPGDDNWLPPDWRTEIRVRTSGTKAGTVDKFYYEPITGRKFRSKNEVLYYL 78 >At5g35330.2 68418.m04188 methyl-CpG-binding domain-containing protein similar to methyl-CpG binding protein MBD4 [Mus musculus] GI:3800807; contains Pfam profile PF01429: Methyl-CpG binding domain Length = 272 Score = 44.8 bits (101), Expect = 6e-05 Identities = 28/68 (41%), Positives = 36/68 (52%), Gaps = 8/68 (11%) Query: 9 RSDCSALPKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYL-------GDSV 61 + + S P GWQR +R G DVYY +P+GKK RS E+ +YL G+ V Sbjct: 122 KPNISRPPAGWQRLLRIRGEG-GTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEYIGEGV 180 Query: 62 DLSCFDFQ 69 LS F FQ Sbjct: 181 KLSQFSFQ 188 >At5g35330.1 68418.m04187 methyl-CpG-binding domain-containing protein similar to methyl-CpG binding protein MBD4 [Mus musculus] GI:3800807; contains Pfam profile PF01429: Methyl-CpG binding domain Length = 272 Score = 44.8 bits (101), Expect = 6e-05 Identities = 28/68 (41%), Positives = 36/68 (52%), Gaps = 8/68 (11%) Query: 9 RSDCSALPKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYL-------GDSV 61 + + S P GWQR +R G DVYY +P+GKK RS E+ +YL G+ V Sbjct: 122 KPNISRPPAGWQRLLRIRGEG-GTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEYIGEGV 180 Query: 62 DLSCFDFQ 69 LS F FQ Sbjct: 181 KLSQFSFQ 188 >At3g63030.1 68416.m07080 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 186 Score = 43.2 bits (97), Expect = 2e-04 Identities = 19/42 (45%), Positives = 29/42 (69%), Gaps = 3/42 (7%) Query: 16 PKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYL 57 PKG++R V+RK K+D YY++PTGKK RS+ E+ ++ Sbjct: 94 PKGFKRSLVLRK---DYSKMDTYYFTPTGKKLRSRNEIAAFV 132 >At4g22745.1 68417.m03282 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 204 Score = 39.9 bits (89), Expect = 0.002 Identities = 17/47 (36%), Positives = 30/47 (63%), Gaps = 3/47 (6%) Query: 16 PKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYLGDSVD 62 P+G++R ++RK K+D YY +PTGKK +S+ E+ ++ + D Sbjct: 121 PRGFKRSLILRK---DYSKMDAYYITPTGKKLKSRNEIAAFIDANQD 164 >At5g59800.1 68418.m07497 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 306 Score = 39.5 bits (88), Expect = 0.002 Identities = 22/47 (46%), Positives = 29/47 (61%), Gaps = 4/47 (8%) Query: 15 LPKGWQREEVVRKTGLSAGKVDVYYYS-PTGKKFRSKPELVRYLGDS 60 LP+GW EEV RK ++ +D YY TGK+FRS + RYL +S Sbjct: 116 LPRGWSVEEVPRK---NSHYIDKYYVERKTGKRFRSLVSVERYLRES 159 Score = 33.1 bits (72), Expect = 0.18 Identities = 20/44 (45%), Positives = 24/44 (54%), Gaps = 4/44 (9%) Query: 15 LPKGWQREEVVRKTGLSAGKVDVYYYSP-TGKKFRSKPELVRYL 57 LP GW EE R+ S+ +D Y P TG KFRS + RYL Sbjct: 182 LPDGWIVEEKPRR---SSSHIDRSYIEPGTGNKFRSMAAVERYL 222 >At5g35338.2 68418.m04194 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429:Methyl-CpG binding domain Length = 155 Score = 35.9 bits (79), Expect = 0.026 Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 2/43 (4%) Query: 15 LPKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYL 57 +P GW R +R S DVYY+ P+G++ RS E+ +L Sbjct: 63 IPTGWSRSVHIRSE--STKFADVYYFPPSGERLRSSAEVQSFL 103 >At4g39680.1 68417.m05614 SAP domain-containing protein contains Pfam domain PF02037: SAP domain Length = 633 Score = 34.3 bits (75), Expect = 0.079 Identities = 30/106 (28%), Positives = 48/106 (45%), Gaps = 9/106 (8%) Query: 50 KPELVRYLGDSVDLSCFDFQQGQINTMLLCKAKKARAQ---FDYR-GVRNDASLVPPIRQ 105 K ELVR L ++ L + +IN+ + A+KA + F G RN + V P+ Sbjct: 37 KEELVRRLDEA--LRAEQEESERINSATVAAAEKANQEPQMFPVTVGDRNQTTPVTPVEA 94 Query: 106 TASIFKQPVTVYKTQD---SKVKTDLKHGTQEKPKQLFWEKRLEGL 148 S PVT KT + +K+ T+ G + P +F E + + Sbjct: 95 AFSTETTPVTAEKTPEPTQTKITTEASAGVETTPAPVFSEPEVNAV 140 >At5g08230.1 68418.m00965 PWWP domain-containing protein putative transcription factor (HUA2) - Arabidopsis thaliana, EMBL:AF116556 Length = 1445 Score = 30.3 bits (65), Expect = 1.3 Identities = 13/27 (48%), Positives = 19/27 (70%) Query: 118 KTQDSKVKTDLKHGTQEKPKQLFWEKR 144 ++ DSKV TDL + +KPK+L EK+ Sbjct: 290 ESSDSKVVTDLNIASSKKPKELLKEKK 316 >At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 163 Score = 30.3 bits (65), Expect = 1.3 Identities = 22/70 (31%), Positives = 33/70 (47%), Gaps = 5/70 (7%) Query: 16 PKGWQREEVVRKTGLSAGKVDVYYYS--PTGKKFRSKPELVRYLGDSVDLSCFDFQQGQI 73 P+G +R VVR + KVDVYY S P K+F+S E+ ++ D + ++ Sbjct: 76 PQGLKRVLVVRTNCV---KVDVYYESLAPRRKRFKSIKEVATFIEDKEEFKDMTLEEVSF 132 Query: 74 NTMLLCKAKK 83 K KK Sbjct: 133 AAPKRLKLKK 142 >At3g15790.1 68416.m01999 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 254 Score = 29.9 bits (64), Expect = 1.7 Identities = 13/42 (30%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Query: 16 PKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYL 57 P W++ K G S K +V + +PTG++ ++ +L +YL Sbjct: 15 PSSWKKLFYPNKVG-SVKKTEVVFVAPTGEEISNRKQLEQYL 55 >At5g47580.1 68418.m05873 expressed protein strong similarity to unknown protein (pir||E71441) Length = 748 Score = 29.5 bits (63), Expect = 2.2 Identities = 14/39 (35%), Positives = 21/39 (53%) Query: 147 GLTACDANGVIGTTSLPKYIKSLGPYTSDATTIQSLATA 185 G+ CDA+G + K + S+G Y + A+ I LA A Sbjct: 2 GILGCDAHGDLNDAEFSKPLPSIGIYVAVASLICGLAMA 40 >At3g01460.1 68416.m00070 PHD finger family protein / methyl-CpG binding domain-containing protein contains Pfam profiles PF00628: PHD-finger (2 copies), PF01429: Methyl-CpG binding domain Length = 2176 Score = 29.1 bits (62), Expect = 3.0 Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 5/65 (7%) Query: 15 LPKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYLGDSV--DLSCFDFQQGQ 72 L GW+ E + L+ ++ Y +P GK F S E+ YLG ++ + SC D + Sbjct: 268 LEDGWRVEF---RQPLNGYQLCAVYCAPNGKTFSSIQEVACYLGLAINGNYSCMDAEIRN 324 Query: 73 INTML 77 N++L Sbjct: 325 ENSLL 329 >At1g70070.1 68414.m08062 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1171 Score = 28.7 bits (61), Expect = 3.9 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 5/81 (6%) Query: 167 KSLGPYTSDATTIQSLATALHVSSQ-PITGQIGSKQAIKDNPGVFLNPEQPLIAAVTITK 225 KS G Y S T+ + + ++ I S +AI L+ +T+ K Sbjct: 653 KSFGNYVSSNVTVAAKQELAEIDNKIEILSSEISDEAIDKKSRKLLSARD--YKEITVLK 710 Query: 226 EDVRRQEERVKRARQRLRQAL 246 E++R EE+ KRA QR R L Sbjct: 711 EELR--EEKRKRAEQRRRMEL 729 >At3g28030.1 68416.m03499 UV hypersensitive protein (UVH3) / DNA-repair protein, putative identical to UV hypersensitive protein [Arabidopsis thaliana] gi|13649704|gb|AAK37472; similar to Swiss-Prot:P14629 DNA-repair protein complementing XP-G cells homolog (Xeroderma pigmentosum group G complementing protein homolog) [Xenopus laevis] Length = 1479 Score = 28.3 bits (60), Expect = 5.2 Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 2/59 (3%) Query: 47 FRSKPELVRYLGDSVDLSCFDFQQGQINTMLLCKAKKARAQFDYRGVRNDASLVPPIRQ 105 F S+ + YL VDLS F G+ + +L K +F++ G + D L+P +++ Sbjct: 1116 FPSEAVISAYLNPQVDLSTEKFSWGKPDLSVL--RKLCWEKFNWNGKKTDELLLPVLKE 1172 >At3g14980.1 68416.m01894 PHD finger transcription factor, putative contains Pfam profile: PF00628 PHD-finger Length = 1189 Score = 28.3 bits (60), Expect = 5.2 Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 2/36 (5%) Query: 25 VRKTGLSAGK--VDVYYYSPTGKKFRSKPELVRYLG 58 + K SAG+ VD +Y SP G+KFR + LG Sbjct: 258 IEKRERSAGRKHVDTFYISPEGRKFREFGSAWKALG 293 >At1g15340.1 68414.m01835 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 384 Score = 28.3 bits (60), Expect = 5.2 Identities = 11/42 (26%), Positives = 25/42 (59%), Gaps = 1/42 (2%) Query: 16 PKGWQREEVVRKTGLSAGKVDVYYYSPTGKKFRSKPELVRYL 57 P W++ ++ G + K ++ + +PTG++ S+ +L +YL Sbjct: 15 PASWKKLFYPKRAG-TPRKTEIVFVAPTGEEISSRKQLEQYL 55 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.316 0.131 0.375 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,586,392 Number of Sequences: 28952 Number of extensions: 224047 Number of successful extensions: 614 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 598 Number of HSP's gapped (non-prelim): 20 length of query: 249 length of database: 12,070,560 effective HSP length: 79 effective length of query: 170 effective length of database: 9,783,352 effective search space: 1663169840 effective search space used: 1663169840 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 58 (27.5 bits)
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