BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001395-TA|BGIBMGA001395-PA|IPR001353|20S proteasome, A and B subunits (192 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 251 6e-68 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 64 1e-11 SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 62 5e-11 SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 48 7e-07 SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 46 5e-06 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 40 2e-04 SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomy... 37 0.002 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 28 0.77 SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po... 26 4.1 SPBC4B4.01c |||fumble family pantothenate kinase |Schizosaccharo... 25 7.2 SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pomb... 25 7.2 SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 25 7.2 >SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces pombe|chr 3|||Manual Length = 204 Score = 251 bits (614), Expect = 6e-68 Identities = 110/192 (57%), Positives = 151/192 (78%), Gaps = 1/192 (0%) Query: 1 MKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNL 60 M G++CVAIA+D R G+Q+ +++ NFPKVF MG Y+GL GLATD QT+++ ++++NL Sbjct: 14 MAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDVQTLYELFRYKVNL 73 Query: 61 YELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLIGCPNEP 120 Y+ +E R ++PKTF+ ++S+ LYE+RFGPYF PV+AG+ DN P++C D IGC + Sbjct: 74 YKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVSN-DNTPFICGFDSIGCIDFA 132 Query: 121 EDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAISGWGAVVYIIEKD 180 EDF+VSGT +EQLYGMCE+++EPNL+PD+LFETISQAL+NA DRD ISGWG VVY+I D Sbjct: 133 EDFIVSGTATEQLYGMCESVYEPNLEPDDLFETISQALLNAQDRDCISGWGCVVYVITAD 192 Query: 181 KITEKHVKTRMD 192 K ++ VK R D Sbjct: 193 KCIKRLVKGRQD 204 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 64.5 bits (150), Expect = 1e-11 Identities = 46/185 (24%), Positives = 87/185 (47%), Gaps = 3/185 (1%) Query: 1 MKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNL 60 ++ +D V +A D R + A + K+ Q+ ++ G A DTQTV LK+ +++ Sbjct: 30 LRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLLKYYLSM 89 Query: 61 YELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLIGCPNEP 120 Y ++ T + + S + Y+ + ++AG D V ++ L G ++ Sbjct: 90 YRIQFGHDPSVHTAATLASEMCYQNK-NMLSAGLIVAGYDEKTGGD-VYSIPLGGSLHK- 146 Query: 121 EDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAISGWGAVVYIIEKD 180 + + G+ S +YG C+A + N+ +E E + A+ A +RD SG + I+ KD Sbjct: 147 QPLAIGGSGSAFIYGFCDANFRENMTQEEAVEFLKNAVALAMERDGSSGGTIRMVILNKD 206 Query: 181 KITEK 185 + K Sbjct: 207 GMERK 211 >SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 62.1 bits (144), Expect = 5e-11 Identities = 32/114 (28%), Positives = 60/114 (52%), Gaps = 3/114 (2%) Query: 3 GQDCVAIATDKRFGIQAQTVSTNF-PKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNLY 61 G +A D R + ++T F P+V ++G L +G G D + +R++ R++LY Sbjct: 21 GDGFAILAGDTR-SVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVKRIQQRIDLY 79 Query: 62 ELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLIG 115 R M ++ + M+ LLY +RF PY++ +AG+D + + + + D +G Sbjct: 80 HDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDK-EGKGEIYSFDPVG 132 >SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 48.4 bits (110), Expect = 7e-07 Identities = 43/177 (24%), Positives = 74/177 (41%), Gaps = 3/177 (1%) Query: 4 QDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNLYEL 63 Q + + D R S KV ++ P L L G A D Q L L++L Sbjct: 70 QHGIVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQL 129 Query: 64 KENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLIGCPNEPEDF 123 + ++ S +LSN+ Y + + ++AG Y + D G + + F Sbjct: 130 RNKELISVSAASKILSNITYSYKGYGLSMGTMLAGTGKGGTALYYIDSD--GTRLKGDLF 187 Query: 124 VVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAISGWGAVVYIIEKD 180 V G+ S YG+ ++ + +L E +++V A RDA SG +Y I+++ Sbjct: 188 SV-GSGSTFAYGVLDSGYRWDLSKQEALYLAQRSIVAATHRDAYSGGSVNLYHIDEN 243 >SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 45.6 bits (103), Expect = 5e-06 Identities = 34/119 (28%), Positives = 57/119 (47%), Gaps = 5/119 (4%) Query: 1 MKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNL 60 +K D V IA D + + ++ ++G VG G +D Q + QRL ++ + Sbjct: 49 LKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQI-QRLLEKLEI 107 Query: 61 YE--LKENRMMRPKTFSAMLSNLLYERR--FGPYFIEPVIAGLDPYDNQPYVCNMDLIG 115 E + ++P LS +LY RR PY+ + ++AG+D + +PYV DL G Sbjct: 108 KEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENKEPYVAFADLRG 166 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 39.9 bits (89), Expect = 2e-04 Identities = 34/163 (20%), Positives = 69/163 (42%), Gaps = 6/163 (3%) Query: 1 MKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNL 60 ++G +C+ I +++ + Q VS NF K+ + + + GL D + + + + Sbjct: 36 LRGNECIVIGVERKNVPKLQNVS-NFQKIAMVDNHVCLAFAGLNADARILIDKARVEAQN 94 Query: 61 YELKENRMMRPKTFSAMLSNLL--YERRFG--PYFIEPVIAGLDPYDNQPYVCNMDLIGC 116 ++L + + + ++ + Y + G P+ + +IAG D DN P V + G Sbjct: 95 HKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFDVGDNTPRVYQTEPAGI 154 Query: 117 PNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALV 159 N + + G S+ E W+ L DE +L+ Sbjct: 155 YNAWKATAI-GRASKAAREYLEKNWKEGLSRDETIHLAVSSLL 196 >SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 194 Score = 37.1 bits (82), Expect = 0.002 Identities = 32/149 (21%), Positives = 62/149 (41%), Gaps = 5/149 (3%) Query: 42 GLATDTQTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYE--RRFGPYFIEPVIAGL 99 G A DT + + ++LY L+ N + P+ ++ L R PY + ++AG Sbjct: 48 GEAGDTTNFAEYIAANISLYTLRHNLNLSPEATASFTRKQLATSLRSRKPYQVNILLAGY 107 Query: 100 DPYDNQPYVCNMDLIG-CPNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQAL 158 + +P + +D + C P + G S + + ++P+L DE + Sbjct: 108 ETNLGKPELFWLDYLATCVRVP--YACQGYSSFYCLSIFDRYYKPDLTIDEAVRIMKLCF 165 Query: 159 VNAADRDAISGWGAVVYIIEKDKITEKHV 187 R I G + +++KD I E ++ Sbjct: 166 DELKKRMPIDFKGFICKVVDKDGIREINI 194 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 28.3 bits (60), Expect = 0.77 Identities = 14/41 (34%), Positives = 21/41 (51%) Query: 5 DCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLAT 45 + VA A D G+ S + +VF++G L VG+ G T Sbjct: 417 EVVAWANDSPVGLAGYLFSKDISRVFRVGEALQVGMVGCNT 457 >SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 25.8 bits (54), Expect = 4.1 Identities = 11/22 (50%), Positives = 15/22 (68%) Query: 148 DELFETISQALVNAADRDAISG 169 DEL +T+S +AAD DA+ G Sbjct: 298 DELLDTLSTTSPDAADDDALQG 319 >SPBC4B4.01c |||fumble family pantothenate kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 25.0 bits (52), Expect = 7.2 Identities = 8/19 (42%), Positives = 17/19 (89%) Query: 13 KRFGIQAQTVSTNFPKVFQ 31 +RFG+++ T++++F KVF+ Sbjct: 281 ERFGLKSTTIASSFGKVFR 299 >SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 25.0 bits (52), Expect = 7.2 Identities = 17/67 (25%), Positives = 30/67 (44%), Gaps = 7/67 (10%) Query: 69 MRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLIGCPNEPEDFVVSGT 128 ++P+ F+A+ + + E +F F PV+A + Y + P DF V+ Sbjct: 8 LQPQRFAALPNPVTAENKFWRSFKVPVVA-------KEYSAITSIHFSAESPYDFAVTSG 60 Query: 129 CSEQLYG 135 Q+YG Sbjct: 61 ARVQIYG 67 >SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 25.0 bits (52), Expect = 7.2 Identities = 21/84 (25%), Positives = 39/84 (46%), Gaps = 6/84 (7%) Query: 23 STNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNLYELKENRM----MRP--KTFSA 76 S+ PK+ ++ +G P ++ + Q+ RL + L N + + P K Sbjct: 81 SSPSPKIDGTAASIGMGFPPMSPNLQSSIPRLTNVSSSSNLSMNTISSTALTPTEKILQK 140 Query: 77 MLSNLLYERRFGPYFIEPVIAGLD 100 + +LLYER+ +E V+A +D Sbjct: 141 RIEDLLYERQNHQQQLEEVLATVD 164 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.321 0.138 0.420 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 883,503 Number of Sequences: 5004 Number of extensions: 35933 Number of successful extensions: 84 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 73 Number of HSP's gapped (non-prelim): 12 length of query: 192 length of database: 2,362,478 effective HSP length: 69 effective length of query: 123 effective length of database: 2,017,202 effective search space: 248115846 effective search space used: 248115846 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 51 (24.6 bits)
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