BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001386-TA|BGIBMGA001386-PA|IPR000842|Phosphoribosyl pyrophosphate synthetase, IPR005946|Ribose-phosphate pyrophosphokinase, IPR000836|Phosphoribosyltransferase (365 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1620.06c |||ribose-phosphate pyrophosphokinase |Schizosaccha... 440 e-124 SPBC3D6.06c |||ribose-phosphate pyrophosphokinase |Schizosacchar... 322 6e-89 SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizo... 244 1e-65 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 29 1.0 SPBC1734.11 |||DNAJ domain protein Mas5 |Schizosaccharomyces pom... 29 1.4 SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 28 2.4 SPAC630.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.2 SPBPJ4664.01 |dps1|SPBPJ694.01|decaprenyl diphosphate synthase s... 27 3.2 SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce... 27 4.2 SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 9.6 SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces po... 26 9.6 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 26 9.6 >SPCC1620.06c |||ribose-phosphate pyrophosphokinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 321 Score = 440 bits (1083), Expect = e-124 Identities = 209/316 (66%), Positives = 262/316 (82%), Gaps = 1/316 (0%) Query: 50 NIKVFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIGESVRGEDVYIVQSGSGE 109 +IK+F+G+SHP+LA+K+ R+G+ LGKV ++SN ET V IGESVR EDV+I+Q+G G Sbjct: 5 SIKIFAGNSHPELAEKVARRIGLSLGKVAVVQYSNRETSVTIGESVRDEDVFILQTGCGS 64 Query: 110 INDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPITAKLVANILSVSGAD 169 IND+LMELLIMINAC+ ASA R+TA+IPCFPYARQDKKDKSRAPITA+LVAN+L +G + Sbjct: 65 INDHLMELLIMINACRSASARRITAIIPCFPYARQDKKDKSRAPITARLVANMLQTAGCN 124 Query: 170 HIITMDLHASQIQGFFDIPVDNLFAEPAVLKWIKENIPDW-KTSIVVSPDAGGAKRVTSI 228 HIITMDLHASQIQGFF++PVDNL+AEP+VL++I+ENI +++VSPDAGGAKR T++ Sbjct: 125 HIITMDLHASQIQGFFNVPVDNLYAEPSVLRYIRENIDTTVNPTVIVSPDAGGAKRATAL 184 Query: 229 ADRLNVEFALIHKERKKANEVASMVLVGDVKDRTAILVDDMADTCGTICHAAEKLIEAGA 288 ADRL+++FALIHKER+KANEV+ MVLVGDV+D+ AILVDDMADTCGT+ AA+ L + GA Sbjct: 185 ADRLDLDFALIHKERQKANEVSRMVLVGDVRDKLAILVDDMADTCGTLGLAAKTLKDNGA 244 Query: 289 TKVYAILTHGIFSGPAISRINNACLEAVVVTNTIPQERHMQECPKIQCIDVSMMLAEAVR 348 VYAI+THGI SG AI IN + LE V+VTNTIP + C KI+ ID+S +LAE +R Sbjct: 245 KAVYAIVTHGILSGKAIKVINESALEKVIVTNTIPHDDKRSLCSKIETIDISGVLAECIR 304 Query: 349 RTHNGESVSYLFSNVP 364 R H+GESVS LFS P Sbjct: 305 RIHHGESVSVLFSVAP 320 >SPBC3D6.06c |||ribose-phosphate pyrophosphokinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 341 Score = 322 bits (790), Expect = 6e-89 Identities = 172/339 (50%), Positives = 227/339 (66%), Gaps = 27/339 (7%) Query: 48 MPNIKVFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIGESVRGEDVYIVQSGS 107 M N+ VF SHP L + I + L +D+G+V KFSN ET V I +SVRG DVYIV S Sbjct: 1 MKNLVVFGTESHPKLTESICEHLCLDIGRVELSKFSNGETSVRIKQSVRGCDVYIVSPAS 60 Query: 108 GEINDNLMELLIMINACKIASASRVTAVIPCFPYARQ-DKKDK-SRAPIT---------- 155 G++ND+LMELLIMI+ACK ASA +VTAV+P FPY+RQ D+K S AP++ Sbjct: 61 GQVNDHLMELLIMISACKTASAKKVTAVLPVFPYSRQPDQKFSFSGAPLSDLQDAVVPCK 120 Query: 156 ------------AKLVANILSVSGADHIITMDLHASQIQGFFDIPVDNLFAEPAVLKWIK 203 LVA++L SGADHIITMDLH Q QGFFDIPVDNLF P + +I Sbjct: 121 KQTGYHPWIAQSGTLVADLLMCSGADHIITMDLHDPQFQGFFDIPVDNLFGRPLLKHYIS 180 Query: 204 ENIPDWKTSIVVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGDVKDRTA 263 NIP++ +++VSPDAGGAKR T+IAD L ++FALIHK R+ S++LVGDV+++ A Sbjct: 181 LNIPNYHNAVIVSPDAGGAKRATAIADALGLDFALIHKNRRH-EYGTSLMLVGDVQNKVA 239 Query: 264 ILVDDMADTCGTICHAAEKLIEAGATKVYAILTHGIFSGPAISRINNACLEAVVVTNTIP 323 IL+DD+ DT T+ AAE + E GA+K+YA++TH + SG AI R+ +C++ ++VTNT P Sbjct: 240 ILIDDLIDTAYTLVRAAEFVKEHGASKIYALVTHCVLSGDAIERVKLSCIDKLIVTNTAP 299 Query: 324 QERHMQECPKIQCIDVSMMLAEAVRRTHNGESVSYLFSN 362 Q C IDV+ AEA+RR HNGES+S L+ + Sbjct: 300 QTITPSGC--FDIIDVAPTFAEAIRRIHNGESISILYDH 336 >SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 244 bits (598), Expect = 1e-65 Identities = 114/200 (57%), Positives = 156/200 (78%) Query: 48 MPNIKVFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIGESVRGEDVYIVQSGS 107 M + + G SHP+L I +RLGI V K+F+N ET VEI ESVR +DV+I+QSGS Sbjct: 1 MKSATIIGGGSHPELLHLISNRLGITPCDVSLKRFANGETSVEIRESVRDKDVFILQSGS 60 Query: 108 GEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPITAKLVANILSVSG 167 +ND+LMELLI+I+ACK SA R+TAV+P FPY++Q K K R ITA++VAN+L+V+G Sbjct: 61 STVNDSLMELLIIISACKGGSAKRITAVMPYFPYSKQSKMRKYRDAITARMVANLLTVAG 120 Query: 168 ADHIITMDLHASQIQGFFDIPVDNLFAEPAVLKWIKENIPDWKTSIVVSPDAGGAKRVTS 227 DHIIT+DLHASQ+QGFF PVDNL+AEP + +WI+ N+ DW+ ++VVS + GGAKRVTS Sbjct: 121 VDHIITLDLHASQMQGFFTRPVDNLYAEPNIAEWIRRNVDDWEEAVVVSKNPGGAKRVTS 180 Query: 228 IADRLNVEFALIHKERKKAN 247 +AD LN++FALI+ +R++++ Sbjct: 181 LADTLNLDFALINTDRQRSS 200 Score = 95.9 bits (228), Expect = 8e-21 Identities = 50/112 (44%), Positives = 73/112 (65%), Gaps = 3/112 (2%) Query: 252 MVLVGDVKDRTAILVDDMADTCGTICHAAEKLIE-AGATKVYAILTHGIFSGPAISRINN 310 + LVGDV +TA+L+DD + A+E L++ GA +V I THGIF + + + Sbjct: 294 ITLVGDVNGKTALLIDDTIENPTAFIVASEHLVKRCGAKRVIVIGTHGIFQNKCLKDLQS 353 Query: 311 A-CLEAVVVTNTIP-QERHMQECPKIQCIDVSMMLAEAVRRTHNGESVSYLF 360 +E +VVTNT P + + + EC K+ ID+S +LAEA+RRTHNGES+S+LF Sbjct: 354 CEYIEQIVVTNTYPIKPQAVLECDKLTVIDISGVLAEAIRRTHNGESISFLF 405 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 29.1 bits (62), Expect = 1.0 Identities = 30/136 (22%), Positives = 56/136 (41%), Gaps = 4/136 (2%) Query: 76 KVVTKKFSNMETCVEIGESV--RGEDVYIVQSGSGEINDNLMELLIMINACKIASASRVT 133 +VVTK S++ C+ G S+ R D+ I + + L+I++ + T Sbjct: 120 QVVTKNTSHLYDCIRSGVSICNRLLDMEIFEPAISLLMKTHKNLIILLTYRDHDAIPTAT 179 Query: 134 AVIPCFPYARQDKKDKSRAP-ITAKLVANILSVSGADHI-ITMDLHASQIQGFFDIPVDN 191 + P + + P + A NI ++ + + L SQI G ++N Sbjct: 180 LLNPTLDVSEIQLESCLFVPMVPASYFLNIGTIVVTFQLNVLRCLSLSQINGLSLNTINN 239 Query: 192 LFAEPAVLKWIKENIP 207 L +E +WI+ + P Sbjct: 240 LQSEDGPFQWIERSFP 255 >SPBC1734.11 |||DNAJ domain protein Mas5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 28.7 bits (61), Expect = 1.4 Identities = 16/58 (27%), Positives = 25/58 (43%), Gaps = 1/58 (1%) Query: 302 GPAISRINNACLEAVVVTNTIPQERHMQECPKIQCIDVSMMLAEAVRR-THNGESVSY 358 GP I R+ C + TI E +EC + I +L V + HNG+ + + Sbjct: 169 GPMIQRMQMTCPDCNGAGETIRDEDRCKECDGAKVISQRKILTVHVEKGMHNGQKIVF 226 >SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 461 Score = 27.9 bits (59), Expect = 2.4 Identities = 19/74 (25%), Positives = 37/74 (50%), Gaps = 4/74 (5%) Query: 170 HIITMDLHASQIQGFFDIPVDNLFA-EPAVLK-WIKENIPDWKTSIVVSPDAGGAKRVTS 227 H +T +L ++I + P D+ F EPA W+++ P+ + + + + G K +S Sbjct: 376 HPLTKNLLFTRIHK--EYPCDSFFPFEPAESSDWVRKAHPELEKFVGIPVEEGRLKAASS 433 Query: 228 IADRLNVEFALIHK 241 + + +EF L K Sbjct: 434 NKEEVEIEFELYGK 447 >SPAC630.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 27.5 bits (58), Expect = 3.2 Identities = 14/42 (33%), Positives = 20/42 (47%) Query: 137 PCFPYARQDKKDKSRAPITAKLVANILSVSGADHIITMDLHA 178 PCF Y + K+ K I + V N S G + +DLH+ Sbjct: 93 PCFSYCARLKEPKDLLEIGSVSVDNKCSTCGLFRVSRIDLHS 134 >SPBPJ4664.01 |dps1|SPBPJ694.01|decaprenyl diphosphate synthase subunit Dps1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 27.5 bits (58), Expect = 3.2 Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 1/56 (1%) Query: 177 HASQIQGFFDIPVDNLFAEPAVLKWIKENIPDWKTSIVVSPDAGGAKRVTSIADRL 232 H S IQ + V+ A + W KE I K S++ PD+ K + ++AD++ Sbjct: 319 HPSDIQRARSL-VECTDAIEQTITWAKEYIKKAKDSLLCLPDSPARKALFALADKV 373 >SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 27.1 bits (57), Expect = 4.2 Identities = 28/123 (22%), Positives = 55/123 (44%), Gaps = 16/123 (13%) Query: 183 GFFDIPVDNLFAEPAVLK-WIKENIPDWKTSIVVSPDAGGAKRVTSIADRLNVEFAL--- 238 GF + N+F+ P LK +++ D SI DA + + A++L V+ L Sbjct: 70 GFEVYTLRNIFSYPPELKGYMRTFGKDVDYSITTEQDAAMDQAIQEAAEKLVVKMQLRRD 129 Query: 239 ----IHKERKKANEVASMV----LVGDVKDRTAILV----DDMADTCGTICHAAEKLIEA 286 + ++R+K E+ + + V + + + D + D G + HA ++++EA Sbjct: 130 LRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDFLLDQLGNLQHAVKRVVEA 189 Query: 287 GAT 289 T Sbjct: 190 SPT 192 >SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1098 Score = 25.8 bits (54), Expect = 9.6 Identities = 9/25 (36%), Positives = 15/25 (60%) Query: 292 YAILTHGIFSGPAISRINNACLEAV 316 Y +L H F+ P +S AC++A+ Sbjct: 594 YPLLEHLAFASPYVSSFAEACIQAI 618 >SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 25.8 bits (54), Expect = 9.6 Identities = 12/35 (34%), Positives = 17/35 (48%) Query: 239 IHKERKKANEVASMVLVGDVKDRTAILVDDMADTC 273 ++K+ KK N + +V DVK R D D C Sbjct: 1 MNKKDKKFNYIFGSEIVNDVKKRLPYYKSDWIDAC 35 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 25.8 bits (54), Expect = 9.6 Identities = 12/29 (41%), Positives = 15/29 (51%) Query: 5 KSNQSDSVASPLATTSGLVSMKSTITLED 33 KS Q +A P T+G V MK+ L D Sbjct: 710 KSKQHTEIAQPAFNTTGTVLMKTKSNLSD 738 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.132 0.375 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,492,522 Number of Sequences: 5004 Number of extensions: 56795 Number of successful extensions: 188 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 173 Number of HSP's gapped (non-prelim): 14 length of query: 365 length of database: 2,362,478 effective HSP length: 74 effective length of query: 291 effective length of database: 1,992,182 effective search space: 579724962 effective search space used: 579724962 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 54 (25.8 bits)
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