BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001383-TA|BGIBMGA001383-PA|IPR005886|UDP-glucose 4-epimerase, IPR001509|NAD-dependent epimerase/dehydratase (356 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 348 6e-97 SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 317 1e-87 SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta 5-->4-i... 53 7e-08 SPBPB2B2.11 |||nucleotide-sugar 4,6-dehydratase |Schizosaccharom... 37 0.005 SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 29 1.3 SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 28 2.3 SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 28 2.3 SPBC1773.04 |||flavonol reductase/cinnamoyl-CoA reductase family... 27 3.1 SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T... 27 4.0 SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 27 4.0 SPBC2A9.02 |||NAD dependent epimerase/dehydratase family protein... 27 5.3 SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 26 7.1 >SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 348 bits (856), Expect = 6e-97 Identities = 182/353 (51%), Positives = 230/353 (65%), Gaps = 19/353 (5%) Query: 5 VLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTGK 64 VLVTGGAGY+GSHT + LLE+ D +V+VDNL N+ + EA+ RIE+LTGK Sbjct: 9 VLVTGGAGYIGSHTCVVLLEKGYD-----VVIVDNLCNS-------RVEAVHRIEKLTGK 56 Query: 65 TVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTLFEI 124 V F++VD+ ALD++F N NI VIHFA LKAVGESV+ PL YY+ NI+GT L E Sbjct: 57 KVIFHQVDLLDEPALDKVFANQNISAVIHFAGLKAVGESVQVPLSYYKNNISGTINLIEC 116 Query: 125 MRKHGVYKLVYSSSCTVYGEPEY----LPINESHPTGKSLTSPYGKSKYFCEEIMKDLCV 180 M+K+ V V+SSS TVYG+P +PI ES P + TSPYG++K F E I++D Sbjct: 117 MKKYNVRDFVFSSSATVYGDPTRPGGTIPIPESCP--REGTSPYGRTKLFIENIIEDETK 174 Query: 181 SDKKWAIISLRYFNPVGAHESGRIGEDPSGIPNNLMPYISQVAVGRLKELKVFGDDYPTA 240 +K LRYFNP GAH SG +GEDP GIPNNL+PYI+QVAVGRL L VFGDDYPT+ Sbjct: 175 VNKSLNAALLRYFNPGGAHPSGELGEDPLGIPNNLLPYIAQVAVGRLDHLNVFGDDYPTS 234 Query: 241 DGTGVRDYIHVCDLAAGHVQAIKLFHQPGFNGFHAINLGTGTGYSVLEMVAAFSNASGXX 300 DGT +RDYIHVCDLA HV A+ Q F NLG+GTG +V +++ AFS A G Sbjct: 235 DGTPIRDYIHVCDLAEAHVAALDYLRQ-HFVSCRPWNLGSGTGSTVFQVLNAFSKAVGRD 293 Query: 301 XXXXXXXXXXGDVTANVADVALATRSLGWRATRSLHDMCRDTWRWQEANPNGY 353 GDV A+ A L W+ +RS++++C DTWRWQ+ P G+ Sbjct: 294 LPYKVTPRRAGDVVNLTANPTRANEELKWKTSRSIYEICVDTWRWQQKYPYGF 346 >SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 317 bits (779), Expect = 1e-87 Identities = 168/355 (47%), Positives = 221/355 (62%), Gaps = 20/355 (5%) Query: 5 VLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTGK 64 +LVTGGAGY+GSHTVI L+ ++++VDNL N+ C + A+ R+E + K Sbjct: 8 ILVTGGAGYIGSHTVIELINHG-----YKVIIVDNLCNS-CYD------AVARVEFIVRK 55 Query: 65 TVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTLFEI 124 ++ F+K+D+R E L +IF+ I VIHFAALKAVGES++ PLEYY NI GT TL + Sbjct: 56 SIKFFKLDLRDKEGLAQIFDTFKIKGVIHFAALKAVGESMKLPLEYYDNNICGTITLLNV 115 Query: 125 MRKHGVYKLVYSSSCTVYGEP----EYLPINESHPTGKSLTSPYGKSKYFCEEIMKDLCV 180 MR+H V +V+SSS TVYG+ +PI ES P T+PYGK+KY E I+KDL Sbjct: 116 MREHRVKTVVFSSSATVYGDATRFDNMIPIPESCPNDP--TNPYGKTKYAIENIIKDLHT 173 Query: 181 SDKKWAIISLRYFNPVGAHESGRIGEDPSGIPNNLMPYISQVAVGRLKELKVFGDDYPTA 240 SD W LRYFNP+GAH SG +GEDP GIPNNL+P+++QVA+GR ++L VFGDDY + Sbjct: 174 SDNTWRGAILRYFNPIGAHPSGLLGEDPLGIPNNLLPFLAQVAIGRREKLLVFGDDYDSH 233 Query: 241 DGTGVRDYIHVCDLAAGHVQAIKLFHQPGFNG--FHAINLGTGTGYSVLEMVAAFSNASG 298 DGT +RDYIHV DLA GH+ A+ ++ + + NLGTG G SV ++ AF G Sbjct: 234 DGTPIRDYIHVVDLAKGHIAALNYLNKINNSEGMYREWNLGTGKGSSVFDIYHAFCKEVG 293 Query: 299 XXXXXXXXXXXXGDVTANVADVALATRSLGWRATRSLHDMCRDTWRWQEANPNGY 353 GDV A A L W+A S+ D CRD W+W NP G+ Sbjct: 294 KDLPYEVVGRRTGDVLNLTASPNRANSELKWKAELSITDACRDLWKWTIENPFGF 348 >SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 52.8 bits (121), Expect = 7e-08 Identities = 47/171 (27%), Positives = 82/171 (47%), Gaps = 23/171 (13%) Query: 4 AVLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTG 63 +VLV G +G++G H + L ER++ L I D N K + EL G Sbjct: 5 SVLVIG-SGFLGGHIIRQLCEREN----LRIAAFDLFENE------------KLLHELHG 47 Query: 64 KTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTLFE 123 + Y D+ ++R+FE + VIH A+ ++ + + Y++VN++GT + + Sbjct: 48 QFT-MYTGDLTKQGDIERVFEEFHPRVVIHTASPV---HNLARDI-YFEVNVDGTANIIK 102 Query: 124 IMRKHGVYKLVYSSSC-TVYGEPEYLPINESHPTGKSLTSPYGKSKYFCEE 173 +K V LVY+SS V+ + + ++ES P + Y +SK E+ Sbjct: 103 ACQKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEK 153 >SPBPB2B2.11 |||nucleotide-sugar 4,6-dehydratase |Schizosaccharomyces pombe|chr 2|||Manual Length = 365 Score = 36.7 bits (81), Expect = 0.005 Identities = 64/261 (24%), Positives = 108/261 (41%), Gaps = 31/261 (11%) Query: 6 LVTGGAGYVGSHTVITLLERQSDGDPL---EIVVVDNLSNAYCAEGEKKPEALKRIEELT 62 L+TGGAG++GS+ + +++ D ++ V N + + ++ +P R E+ Sbjct: 13 LITGGAGFIGSNFLDYAVDKYPDFHFTCIDKLSYVSNYTTVFLSKVLNQPNF--RFLEMD 70 Query: 63 GKTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTLF 122 T + + ++ E + I +I+FAA +V S PL + + NI T L Sbjct: 71 LATNYKFLYQFMVEDS-----EINKITHIINFAAESSVDRSFIDPLYFTKNNILSTQNLL 125 Query: 123 EIMR--------KHGVYKLVYSSSCTVYGE-PEYLPINESHPTGKSLTSPYGKSKYFCEE 173 E +R V+ S+ VYGE E ++E TSPY SK + Sbjct: 126 ECVRILLGKKEELRNRLNFVHVSTDEVYGEQDENASVDEKSKLNP--TSPYAASKAAVDL 183 Query: 174 IMKDLCVSDKKWAIISLRYFNPVGAHESGRIGEDPSGIPNNLMPYISQVAVGRLKELKVF 233 I++ S K ++ +R N G + P + L +I+Q K K+ Sbjct: 184 IIQSYRYS-YKISVTVIRANNVYGPRQYEE-KLIPMTL-GKLKKFINQ------KSQKIM 234 Query: 234 GDDYPT-ADGTGVRDYIHVCD 253 D DG R Y+H+ D Sbjct: 235 QDKITLHGDGLHKRKYLHIYD 255 >SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 28.7 bits (61), Expect = 1.3 Identities = 13/48 (27%), Positives = 22/48 (45%) Query: 97 LKAVGESVEKPLEYYQVNINGTCTLFEIMRKHGVYKLVYSSSCTVYGE 144 ++A GE +E+ + Y+ N NG C + E C+ YG+ Sbjct: 267 MEAFGEEIEEVVASYEKNYNGRCYISEEACSKATNDCSGHGRCSKYGQ 314 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 27.9 bits (59), Expect = 2.3 Identities = 21/44 (47%), Positives = 24/44 (54%), Gaps = 5/44 (11%) Query: 6 LVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGE 49 +V GAG VGS T TLL G EIV++D N AEGE Sbjct: 23 IVIVGAGNVGSTTAFTLL---LSGLAAEIVIID--LNKKKAEGE 61 >SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 27.9 bits (59), Expect = 2.3 Identities = 10/23 (43%), Positives = 17/23 (73%) Query: 95 AALKAVGESVEKPLEYYQVNING 117 A L +GE++++ +E Y+V ING Sbjct: 243 ARLNEIGEAIQEVMESYEVEING 265 >SPBC1773.04 |||flavonol reductase/cinnamoyl-CoA reductase family|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 27.5 bits (58), Expect = 3.1 Identities = 12/25 (48%), Positives = 16/25 (64%) Query: 1 MSRAVLVTGGAGYVGSHTVITLLER 25 MS VL+TG G+V SH+ LL + Sbjct: 1 MSELVLITGITGFVASHSAEALLSQ 25 >SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 988 Score = 27.1 bits (57), Expect = 4.0 Identities = 17/55 (30%), Positives = 29/55 (52%), Gaps = 1/55 (1%) Query: 181 SDKKWAIISLRYFNPVGAHESGRIGEDPSGIPNNLMPYISQVAVGR-LKELKVFG 234 S+KK + + + PV + I E+ SGIP NL Y+ + + L + ++FG Sbjct: 527 SNKKMLVSTNQTLRPVASILKHLINEESSGIPFNLEVYMKLLTFKKDLLKHRMFG 581 >SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 27.1 bits (57), Expect = 4.0 Identities = 34/157 (21%), Positives = 61/157 (38%), Gaps = 15/157 (9%) Query: 3 RAVLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELT 62 + VLVTG G++G+H LL+ G + V EK E ++ L Sbjct: 4 KLVLVTGVTGFIGAHVAEQLLQA---GYRVRGTV---------RSMEKADELIRLNPGLK 51 Query: 63 GKTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTLF 122 K D+ + A D + + +++ + H A+ V + + + GT + Sbjct: 52 DKIEFVIVKDVSASNAFDGVLK--DVELICHIASPFFVENVTDNKSQLLDPAVKGTLGIL 109 Query: 123 EIMR-KHGVYKLVYSSSCTVYGEPEYLPINESHPTGK 158 E + + ++V +SS G + P N T K Sbjct: 110 EAAQGVKSIKRIVITSSFAAVGNFQIDPHNNKVYTEK 146 >SPBC2A9.02 |||NAD dependent epimerase/dehydratase family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 295 Score = 26.6 bits (56), Expect = 5.3 Identities = 11/20 (55%), Positives = 14/20 (70%) Query: 5 VLVTGGAGYVGSHTVITLLE 24 + VTG AG++GS V LLE Sbjct: 3 IFVTGAAGFIGSEIVRQLLE 22 >SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 677 Score = 26.2 bits (55), Expect = 7.1 Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 4/74 (5%) Query: 64 KTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALK---AVGESVEKPLEYYQVNINGTCT 120 K H +R E ++RI +H IH A+ A+ + PLE + + +N Sbjct: 132 KVFHIKSSSLRKKEIIERILLDH-WKLRIHGDAMDDMLAIKDVTLCPLEMFFLLLNNASA 190 Query: 121 LFEIMRKHGVYKLV 134 L +I +KH + ++ Sbjct: 191 LRDISQKHAAHVVI 204 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.319 0.136 0.418 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,669,055 Number of Sequences: 5004 Number of extensions: 70233 Number of successful extensions: 133 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 116 Number of HSP's gapped (non-prelim): 13 length of query: 356 length of database: 2,362,478 effective HSP length: 74 effective length of query: 282 effective length of database: 1,992,182 effective search space: 561795324 effective search space used: 561795324 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 54 (25.8 bits)
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