BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001383-TA|BGIBMGA001383-PA|IPR005886|UDP-glucose 4-epimerase, IPR001509|NAD-dependent epimerase/dehydratase (356 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_30795| Best HMM Match : Epimerase (HMM E-Value=0) 381 e-106 SB_42322| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 2e-08 SB_32547| Best HMM Match : Epimerase (HMM E-Value=3.2e-08) 46 4e-05 SB_15042| Best HMM Match : No HMM Matches (HMM E-Value=.) 43 4e-04 SB_45592| Best HMM Match : Epimerase (HMM E-Value=1.4e-19) 38 0.016 SB_32433| Best HMM Match : NAD_binding_4 (HMM E-Value=0.037) 37 0.029 SB_45640| Best HMM Match : Epimerase (HMM E-Value=9.4e-38) 36 0.038 SB_27528| Best HMM Match : Epimerase (HMM E-Value=3.2e-06) 31 1.4 SB_37976| Best HMM Match : Tubulin (HMM E-Value=4.5e-18) 30 2.5 SB_11668| Best HMM Match : rve (HMM E-Value=6.2e-14) 29 4.4 SB_28495| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.8 SB_23844| Best HMM Match : RRM_2 (HMM E-Value=4.8) 29 7.6 SB_14386| Best HMM Match : Ion_trans_2 (HMM E-Value=2.4e-11) 29 7.6 >SB_30795| Best HMM Match : Epimerase (HMM E-Value=0) Length = 869 Score = 381 bits (937), Expect = e-106 Identities = 187/339 (55%), Positives = 238/339 (70%), Gaps = 15/339 (4%) Query: 1 MSRAVLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEE 60 MS+ VLVTGGAGY+GSH V+ +L E+V++DNLSN+ E ++R+EE Sbjct: 37 MSKKVLVTGGAGYIGSHCVVEILNAG-----YEVVIIDNLSNS-------DAECVRRVEE 84 Query: 61 LTGKTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCT 120 ++GK V F+ D+ EALD IF+ H + V+HFA LKAVGESV+ PL YY N+ GT Sbjct: 85 ISGKKVPFFIEDLLNKEALDDIFKKHKFNGVLHFAGLKAVGESVQIPLRYYHNNLTGTLH 144 Query: 121 LFEIMRKHGVYKLVYSSSCTVYGEPEYLPINESHPTGKSLTSPYGKSKYFCEEIMKDLCV 180 L E M+KHGV+ LV+SSS TVYG+P++LPI ESHP G T+PYGK+KYF EE++KDLC Sbjct: 145 LLECMKKHGVHNLVFSSSATVYGDPQFLPITESHPAG-GCTNPYGKTKYFIEEMLKDLCY 203 Query: 181 SDKKWAIISLRYFNPVGAHESGRIGEDPSGIPNNLMPYISQVAVGRLKELKVFGDDYPTA 240 ++K++ I+SLRYFNPVGAH+SG+IGEDP IPNNL+PYI+QVAVG+ L VFG DY T Sbjct: 204 AEKEFNIVSLRYFNPVGAHKSGKIGEDPKDIPNNLLPYITQVAVGKRPHLNVFGKDYDTP 263 Query: 241 DGTGVRDYIHVCDLAAGHVQAIKLFHQPGFNGFHAINLGTGTGYSVLEMVAAFSNASGXX 300 DGTGVRDYIHV DLA GHV A+K + G+ NLGTG GYSVLEMV A ASG Sbjct: 264 DGTGVRDYIHVVDLAMGHVAALKKVEEK--CGWKVYNLGTGRGYSVLEMVKAVEKASGKE 321 Query: 301 XXXXXXXXXXGDVTANVADVALATRSLGWRATRSLHDMC 339 GD+ + AD +LA + L W+ATR+ ++MC Sbjct: 322 IPYKFTDRRAGDIASCYADPSLAAKELNWKATRAQNEMC 360 >SB_42322| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 294 Score = 57.2 bits (132), Expect = 2e-08 Identities = 40/135 (29%), Positives = 62/135 (45%), Gaps = 1/135 (0%) Query: 66 VHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTLFEIM 125 ++ K DI A L IF+ ID V+HFAA V S L++ + N+ GT L + Sbjct: 50 INLDKGDICEANHLKYIFQAEQIDTVLHFAAQSHVDNSFWSSLDFTKTNVYGTHVLINVA 109 Query: 126 RKHGVYKLVYSSSCTVYGEPEYLPINESHPTGKSLTSPYGKSKYFCEEIMKDLCVSDKKW 185 + + K ++ S+ VYG L S + ++PY SK E I+ + K+ Sbjct: 110 HEAKIKKFIHVSTDEVYGGNSSLGDMHSESSPLRPSNPYAASKAAAECIVMSY-LESFKF 168 Query: 186 AIISLRYFNPVGAHE 200 +I R N G H+ Sbjct: 169 PVIITRSNNVYGPHQ 183 >SB_32547| Best HMM Match : Epimerase (HMM E-Value=3.2e-08) Length = 327 Score = 46.4 bits (105), Expect = 4e-05 Identities = 35/102 (34%), Positives = 52/102 (50%), Gaps = 5/102 (4%) Query: 72 DIRCAEALDRIFENHNIDCVIHFAA-LKAVGESVEKPLEYYQVNINGTCTLFEIMRKHGV 130 D+ ++L I NHNID ++HF+A L AVGE + + QVNI G + E RK+ + Sbjct: 80 DVLDPQSLQSIVVNHNIDWIVHFSAILSAVGE--QNVTQALQVNIEGFHNIIETCRKYNL 137 Query: 131 YKLVYSSSCTVYGEPEYLPINESHPTGKSLTSPYGKSKYFCE 172 +L S+ +G PE N T + + YG +K E Sbjct: 138 -RLFSPSTIGAFG-PETPMDNTPDITIQRPKTIYGVAKVHME 177 >SB_15042| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 45 Score = 42.7 bits (96), Expect = 4e-04 Identities = 23/42 (54%), Positives = 26/42 (61%), Gaps = 2/42 (4%) Query: 257 GHVQAIKLFHQPGFNGFHAINLGTGTGYSVLEMVAAFSNASG 298 GHV A+K + G+ NLGTG GYSVLEMV A ASG Sbjct: 2 GHVAALKKVEEKC--GWKVYNLGTGRGYSVLEMVKAVEKASG 41 >SB_45592| Best HMM Match : Epimerase (HMM E-Value=1.4e-19) Length = 672 Score = 37.5 bits (83), Expect = 0.016 Identities = 47/175 (26%), Positives = 82/175 (46%), Gaps = 22/175 (12%) Query: 5 VLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTGK 64 VLVTG +G++ H V LLE +G + V +LS +KK + L+ + + Sbjct: 18 VLVTGASGFIACHVVKQLLE---EGKFIVRGTVRDLS------VDKKVQPLRNLCPDSK- 67 Query: 65 TVHFYKVDIRCAEALDRI-FENHNIDC--VIHFAA-LKAVGESVEKPLEYYQVNINGTCT 120 Y ++I A+ +D + +E DC V+H A+ A +E + + + GT + Sbjct: 68 ----YPLEIVEADLMDEVCWERAAKDCQYVLHMASPFPASNPKLES--DIIEPAVEGTRS 121 Query: 121 LFEIMRKHGVYKLVYSSSCTV--YGEPEYLPINESHPTGKSLTSPYGKSKYFCEE 173 + + K GV ++V +SS +G + + E + +S PY KSK E+ Sbjct: 122 VLKACAKCGVKRVVLTSSIAAVSFGHDDSRVLTEEDWSIESECFPYAKSKLLAEK 176 >SB_32433| Best HMM Match : NAD_binding_4 (HMM E-Value=0.037) Length = 116 Score = 36.7 bits (81), Expect = 0.029 Identities = 32/117 (27%), Positives = 50/117 (42%), Gaps = 10/117 (8%) Query: 5 VLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTGK 64 +LVTGG G VGSH + L + L I + + ++EL K Sbjct: 2 ILVTGGTGLVGSHLLYELALKN-----LPIKAIYRTKESLATVKRVFSYFTTNVDELFNK 56 Query: 65 TVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCTL 121 + + + DI +LD F+ + V H AAL + + + QVNI+GT + Sbjct: 57 -IQWIQADITDVPSLDEAFK--EVTLVYHAAALVSFNSKDYRAMR--QVNIDGTANM 108 >SB_45640| Best HMM Match : Epimerase (HMM E-Value=9.4e-38) Length = 451 Score = 36.3 bits (80), Expect = 0.038 Identities = 52/176 (29%), Positives = 78/176 (44%), Gaps = 31/176 (17%) Query: 5 VLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDN----LSNAY-CAEGEKKPEALKRIE 59 VLVTGGAGY+GS V LL++ ++ V D +S+ Y CA R++ Sbjct: 143 VLVTGGAGYLGSSLVPILLDQG-----YKVTVYDRFLWGISSLYPCASNP-------RLQ 190 Query: 60 ELTGKTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTC 119 + G DI L + D VIH A++ + P + +VN GT Sbjct: 191 IING--------DILDVGHLSQCISE--CDAVIHLASIVGYPACEKDPQKATEVNEQGTR 240 Query: 120 TLFEIMRKHGVYKLVYSSSCTVYGEPEYLPINESHPTGKSLTSPYGKSKYFCEEIM 175 + + + LVY+S+ + YG E ES P S + YGK+K EE++ Sbjct: 241 NVVDALLPG--QPLVYASTGSCYGAIEDGLCTESTPI--SPLTLYGKTKANGEEMV 292 >SB_27528| Best HMM Match : Epimerase (HMM E-Value=3.2e-06) Length = 441 Score = 31.1 bits (67), Expect = 1.4 Identities = 43/177 (24%), Positives = 74/177 (41%), Gaps = 23/177 (12%) Query: 2 SRAVLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEK-KPEALKRIEE 60 +R VLV GG G++GS V L+++ GD + IV N N Y E+ KP Sbjct: 25 TRRVLVFGGNGFIGSEVVSRLIKQ---GDDITIV---NRGNWYFDSKERIKPYTSTHYRC 78 Query: 61 LTGKTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGESVEKPLEYYQVNINGTCT 120 K +H + C E L F D V+ F++ ++ +++ LE ++ + Sbjct: 79 DRDKALH-----LECPELLTSGF----YDIVLDFSSYTSL--QIKQVLETFKERVG---- 123 Query: 121 LFEIMRKHGVYKLVYSSSCTVYGEPEYL-PINESHPTGKSLTSPYGKSKYFCEEIMK 176 L+ + VY++ E + + P + + YG K CEE ++ Sbjct: 124 LYIYISSDSVYEVCDKKHKGPSREEDAVRPKSPKKRLKLKKSEEYGHEKLACEEALR 180 >SB_37976| Best HMM Match : Tubulin (HMM E-Value=4.5e-18) Length = 553 Score = 30.3 bits (65), Expect = 2.5 Identities = 19/57 (33%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Query: 1 MSRAVLVTGGAGYVGSHTVITLLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKR 57 MS +L G G H ++TL+E+Q +G PL D C + E K A R Sbjct: 1 MSSILLPVGQCGNQIGHELMTLVEKQCEGKPLS--HRDGKLRCVCVDSESKVIASNR 55 >SB_11668| Best HMM Match : rve (HMM E-Value=6.2e-14) Length = 597 Score = 29.5 bits (63), Expect = 4.4 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 3/54 (5%) Query: 22 LLERQSDGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTGKTVHFYKVDIRC 75 LL + PL+I+V S A+ A+G + +A+K + L G+ YKV + C Sbjct: 413 LLNGRQIRTPLDILVP---SPAHQAQGRQDKQAVKAAQRLAGRQDRRYKVGMAC 463 >SB_28495| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 6753 Score = 29.1 bits (62), Expect = 5.8 Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 3/60 (5%) Query: 28 DGDPLEIVVVDNLSNAYCAEGEKKPEALKRIEELTGKTVHFYKVDIRCAEALDRIFENHN 87 DG PLE V DNL++ E +K P+ + + + + Y V C E L+ I ++ N Sbjct: 6473 DGLPLENFVDDNLNDDDDTEAQKTPQRYMLLANVINEQMKQYAV---CNEDLNEIQQDMN 6529 >SB_23844| Best HMM Match : RRM_2 (HMM E-Value=4.8) Length = 190 Score = 28.7 bits (61), Expect = 7.6 Identities = 18/63 (28%), Positives = 31/63 (49%), Gaps = 2/63 (3%) Query: 53 EALKRIEELTGKTVHFYKVDIRCAEALDRIFENHNIDCVIHFAALKAVGES-VEKPLEYY 111 E+LK L+ +HFY + CA L ++ N+ ++H K +G++ V E Sbjct: 34 ESLKNNSTLSTIGIHFYSLGDACASELAKVLVNNTSLNIVHIGG-KYLGDAGVASIAEAL 92 Query: 112 QVN 114 +VN Sbjct: 93 KVN 95 >SB_14386| Best HMM Match : Ion_trans_2 (HMM E-Value=2.4e-11) Length = 556 Score = 28.7 bits (61), Expect = 7.6 Identities = 30/104 (28%), Positives = 43/104 (41%), Gaps = 9/104 (8%) Query: 149 PINESHPTGKSLTSPYGKSKYFCEEIMKDLCV-----SDKKWAIISLRYFNPVGAHESGR 203 P E+ K T P GK KYF K DK + ++ + +GA SG Sbjct: 409 PKLETKEIEKEFTKPSGKIKYFLGHDYKKAQTYTNRYVDKSQLVRNIEHQFSIGAVVSGN 468 Query: 204 IGEDPSGIPNNLMPYISQVAVGRLKELKVFGDDYPT-ADGTGVR 246 G I +A+ +E KV G + PT +DGTG++ Sbjct: 469 FGIAQDFILCLHREITDMIAI---EEAKVTGVEDPTNSDGTGLQ 509 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.319 0.136 0.418 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,450,102 Number of Sequences: 59808 Number of extensions: 516477 Number of successful extensions: 953 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 942 Number of HSP's gapped (non-prelim): 15 length of query: 356 length of database: 16,821,457 effective HSP length: 83 effective length of query: 273 effective length of database: 11,857,393 effective search space: 3237068289 effective search space used: 3237068289 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 61 (28.7 bits)
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