Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001364-TA|BGIBMGA001364-PA|IPR007234|Vps53-like,
N-terminal
(801 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g50500.1 68414.m05664 membrane trafficking VPS53 family prote... 227 2e-59
At1g50970.1 68414.m05730 membrane trafficking VPS53 family prote... 175 1e-43
At1g67320.1 68414.m07662 DNA primase, large subunit family conta... 32 1.7
At5g07660.1 68418.m00877 structural maintenance of chromosomes (... 31 2.3
At1g41820.1 68414.m04825 hypothetical protein 31 3.0
At2g03050.1 68415.m00258 mitochondrial transcription termination... 31 4.0
At2g46090.1 68415.m05733 diacylglycerol kinase family protein co... 30 5.3
At5g18660.1 68418.m02215 isoflavone reductase-related low simila... 29 9.2
>At1g50500.1 68414.m05664 membrane trafficking VPS53 family protein
contains Pfam domain PF04100: Vps53-like, N-terminal
Length = 798
Score = 227 bits (555), Expect = 2e-59
Identities = 139/420 (33%), Positives = 225/420 (53%), Gaps = 29/420 (6%)
Query: 41 DDFDAVAYINRVFPTEQSLSGVESAVARCEFRLSGVEQDIRRLVXXXXXXXXXXXXXLTE 100
D A+ YIN++FPTE SL+GVE + + + + V+ I V L +
Sbjct: 2 DKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLAD 61
Query: 101 AQRCIAELALQVADINKKAERSESMVREITCEIKQLDCAKCNLTAAITALNHLHMLAGGV 160
A R + EL+ ++ +I KAE+SE+MV+EI +IK+LD AK N+T ITAL+ L ML V
Sbjct: 62 ATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAV 121
Query: 161 DTLTTMTKNRQYKEIALPMQAIMEVLQHFECYRDIRELSVLRDQVHSIRHDLGNQILADF 220
+ L M RQYKE A ++A+ ++ HFE YRD+ +++ LR+++++I+ L + + +DF
Sbjct: 122 EQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDF 181
Query: 221 KQAFTG--GSKSNITHRTLSEACGVVDILDPKVKQDLLKWFISMQLQEYQHLYSSEQECA 278
TG ++N+ + LS++C VVD L+P V+++L+ F S +L Y+ ++ E A
Sbjct: 182 SSLGTGKETEETNLLQK-LSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEG-AELA 239
Query: 279 WLTHIERRYAWLKKHLLTFEDTLGNIFPYQWALSENIARQFCKITSTELSNLMAARRNEL 338
L ERRYAW+K+ + T E+ + IFP W + + QFCK T ++ +++ + +
Sbjct: 240 KLDKTERRYAWIKRRIRTNEE-IWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKP 298
Query: 339 DVKLLLYAIQNTYNFELLLHKRFTG----TDLKPD------------NVDT-----NSKQ 377
V +LL A+Q+T FE L K+F G D++ D N+ K
Sbjct: 299 VVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKF 358
Query: 378 PFDNETKDN--TADSDGQQAEGSSSSWVGLIGSCFEPXXXXXXXXXXXXXXXXXERFVQD 435
ET++N T + D G+ ++ G+I SCFEP E+ VQ+
Sbjct: 359 AASQETEENEKTGNKD-LSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQE 417
Score = 150 bits (363), Expect = 4e-36
Identities = 80/239 (33%), Positives = 140/239 (58%), Gaps = 9/239 (3%)
Query: 550 ITSVITTSEYCLETTIHLEQKLKEKVSPKLSDKIDLTPEQDLFHKIISNCIQMLVQDLET 609
I ++ ++EYC +T+ L + + E + P +D +D++ QD F +I+ + LV LET
Sbjct: 481 ICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLET 540
Query: 610 ACEPALQSMTKISWLSFDNVGDQSSYVTQIIMHLKNTVPNLRDNLSSSRKYFTQFCIRFA 669
+ + MT++ W + ++VGDQS YV I L ++P L L+ YF F + A
Sbjct: 541 KFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTP--VYFQFFLDKLA 598
Query: 670 NSFIPKFIQNVYKCKPISTVGSEQLLLDTHMLKTALLELPSIGSEVKRAAPATYTKVVLK 729
+S P+F N+++CK +S G++Q+LLDT +K+ LLE+PS+ + A A+Y+K V +
Sbjct: 599 SSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTSTA--ASYSKFVSR 656
Query: 730 LMTKAEMVLKLVMAPLEGNLEGFVTQFVKLLPDCSLAEFHKVLDMKGAKLSKTQMSNLD 788
M++AE +LK++++P ++ + L P+ + EF ++L++KG K Q S LD
Sbjct: 657 EMSRAEALLKVILSP----IDSVADTYRALFPEGTPMEFQRILELKGLK-KADQQSILD 710
>At1g50970.1 68414.m05730 membrane trafficking VPS53 family protein
contains Pfam domain PF04100: Vps53-like, N-terminal
Length = 569
Score = 175 bits (426), Expect = 1e-43
Identities = 105/324 (32%), Positives = 170/324 (52%), Gaps = 3/324 (0%)
Query: 41 DDFDAVAYINRVFPTEQSLSGVESAVARCEFRLSGVEQDIRRLVXXXXXXXXXXXXXLTE 100
D + +IN++FP E SLS VES + + + V+ I +V L +
Sbjct: 2 DKSSGLDFINQMFPVEASLSCVESFMQKICDEIRRVDATILAVVSQQGNSGTRAKENLND 61
Query: 101 AQRCIAELALQVADINKKAERSESMVREITCEIKQLDCAKCNLTAAITALNHLHMLAGGV 160
A EL+ ++ +I KAE++E+MV++I +IK+LD AK N+T A+TAL+ L ML V
Sbjct: 62 AICAAEELSHKIQEIKSKAEQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSAV 121
Query: 161 DTLTTMTKNRQYKEIALPMQAIMEVLQHFECYRDIRELSVLRDQVHSIRHDLGNQILADF 220
L MT RQYKE A ++AI E+ HF+ Y D+ ++ LR+++ +I+ L + +DF
Sbjct: 122 QQLQVMTSKRQYKEAATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQILKFHVFSDF 181
Query: 221 KQAFTGGSKSNI-THRTLSEACGVVDILDPKVKQDLLKWFISMQLQEYQHLYSSEQECAW 279
TG + + LS++C VVD L+P V+++L+ F S +L Y+ +Y E
Sbjct: 182 SSLGTGTETEELFLLKKLSDSCLVVDALEPSVREELINNFCSRELTSYEQIYVG-AELKT 240
Query: 280 LTHIERRYAWLKKHLLTFEDTLGNIFPYQWALSENIARQFCKITSTELSNLMAARRNELD 339
L IE Y L L+ IFP W + + Q + T ++ +++ + + D
Sbjct: 241 LDEIELIYNQL-SCLIRKNQGKWTIFPASWHVPYRLCIQLSRKTRVQVESILVNLKEKSD 299
Query: 340 VKLLLYAIQNTYNFELLLHKRFTG 363
V+ LL ++ T FE L +F G
Sbjct: 300 VEKLLLELKRTLEFERELEMKFGG 323
Score = 47.6 bits (108), Expect = 3e-05
Identities = 32/106 (30%), Positives = 58/106 (54%), Gaps = 12/106 (11%)
Query: 694 LLLDTHMLKTALLELPSIGSEVK------RAAPATYTKVVLKLMTKAEMVLKLVMAPLEG 747
+LLD H +K LL++PS+ + + + A A+Y K+V M +AE VLK++ +P
Sbjct: 397 MLLDAHDMKMILLKVPSLARQPEASALLVKTATASYVKLVNHQMKRAEAVLKVIASP--- 453
Query: 748 NLEGFVTQFVKLLPDCSLAEFHKVLDMKGAKLSKTQMSNLDAVFKN 793
+ + + L P+ + EF ++L +KG L+K + ++ F N
Sbjct: 454 -IVTVIDTYRALFPEETPMEFQRILVLKG--LTKAEQQSILDDFNN 496
>At1g67320.1 68414.m07662 DNA primase, large subunit family contains
Pfam profile PF04104: Eukaryotic-type DNA primase, large
subunit; similar to DNA primase large subunit (EC
2.7.7.-) (DNA primase 58 kDa subunit) (p58)
(Swiss-Prot:P49643) [Homo sapiens]
Length = 449
Score = 31.9 bits (69), Expect = 1.7
Identities = 22/86 (25%), Positives = 44/86 (51%), Gaps = 4/86 (4%)
Query: 254 DLLKWFISMQLQEYQH---LYSSEQECAWLTHIERRY-AWLKKHLLTFEDTLGNIFPYQW 309
+L KWF+SM+ ++H L E++ A + Y A + L + ++ LG +
Sbjct: 104 ELKKWFLSMETALFRHRFRLKKIEEQRAIVGEFGLPYKAVIGAELESLKERLGLVARSHG 163
Query: 310 ALSENIARQFCKITSTELSNLMAARR 335
+S ++ + K+ E+ +L+A+RR
Sbjct: 164 QISSDVENIYYKVPFEEVPDLVASRR 189
>At5g07660.1 68418.m00877 structural maintenance of chromosomes
(SMC) family protein similar to SMC-like protein (MIM)
[Arabidopsis thaliana] GI:5880614; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain
Length = 1058
Score = 31.5 bits (68), Expect = 2.3
Identities = 19/92 (20%), Positives = 42/92 (45%), Gaps = 1/92 (1%)
Query: 56 EQSLSGVESAVARCEFRLSGVEQDI-RRLVXXXXXXXXXXXXXLTEAQRCIAELALQVAD 114
E +L G+ES + R + + + +E+D+ R+ + + EL L++
Sbjct: 666 EMNLEGLESTMRRLKKQRTQLEKDLTRKELEMQDLKNSVASETKASPTSSVNELHLEIMK 725
Query: 115 INKKAERSESMVREITCEIKQLDCAKCNLTAA 146
K+ E ES++ ++ +K+ + L A+
Sbjct: 726 FQKEIEEKESLLEKLQDSLKEAELKANELKAS 757
>At1g41820.1 68414.m04825 hypothetical protein
Length = 401
Score = 31.1 bits (67), Expect = 3.0
Identities = 17/46 (36%), Positives = 24/46 (52%)
Query: 701 LKTALLELPSIGSEVKRAAPATYTKVVLKLMTKAEMVLKLVMAPLE 746
LK A LPS + K A P+ +TK L K + VL+++ LE
Sbjct: 164 LKKAKHPLPSNSKKAKIALPSEFTKDTCDLYEKTDFVLEIIKLVLE 209
>At2g03050.1 68415.m00258 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 283
Score = 30.7 bits (66), Expect = 4.0
Identities = 21/91 (23%), Positives = 47/91 (51%), Gaps = 4/91 (4%)
Query: 579 LSDKIDLTPEQDLFHKIISNCIQMLVQDLETACEPALQSMTKISWLSFDNVGDQSS--YV 636
LS++I ++ EQD+ K IS C ++L+ ++ PAL + + ++ D + +++ V
Sbjct: 107 LSNEISIS-EQDI-PKSISRCPRLLISSVDYQLRPALTFLKTLGFVGRDTITSRNTVLLV 164
Query: 637 TQIIMHLKNTVPNLRDNLSSSRKYFTQFCIR 667
+ + L + L + L +R+ + +R
Sbjct: 165 SNVERTLIPKIEYLEEGLGFTREEVAKMVVR 195
>At2g46090.1 68415.m05733 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 364
Score = 30.3 bits (65), Expect = 5.3
Identities = 14/32 (43%), Positives = 19/32 (59%)
Query: 351 YNFELLLHKRFTGTDLKPDNVDTNSKQPFDNE 382
Y+F L LHK + GT L +NV + S Q + E
Sbjct: 298 YDFVLKLHKLYNGTHLSVNNVTSRSVQSIEVE 329
>At5g18660.1 68418.m02215 isoflavone reductase-related low
similarity to SP|P52575 Isoflavone reductase (EC
1.3.1.-) (IFR) {Medicago sativa}
Length = 417
Score = 29.5 bits (63), Expect = 9.2
Identities = 20/90 (22%), Positives = 40/90 (44%), Gaps = 2/90 (2%)
Query: 683 CKPISTVGSEQLLLDTHMLKTALLELPSIGSEVKRAAPATYTKVVLKLMTKAEMVLKLVM 742
CKPIS + D + + + ++ IG K P +++ K++ + LK+ +
Sbjct: 275 CKPISEQDLAAFIADCVLEENKINQVLPIGGPGKALTPLEQGEILFKILGREPKFLKVPI 334
Query: 743 APLEGNLEGFVTQFVKLLPDC-SLAEFHKV 771
++ + G + K+ P AEF K+
Sbjct: 335 EIMDFVI-GVLDSIAKIFPSVGEAAEFGKI 363
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.131 0.376
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,915,053
Number of Sequences: 28952
Number of extensions: 663019
Number of successful extensions: 1790
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1772
Number of HSP's gapped (non-prelim): 12
length of query: 801
length of database: 12,070,560
effective HSP length: 87
effective length of query: 714
effective length of database: 9,551,736
effective search space: 6819939504
effective search space used: 6819939504
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)
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