BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001344-TA|BGIBMGA001344-PA|IPR011009|Protein kinase-like, IPR002290|Serine/threonine protein kinase, IPR000719|Protein kinase, IPR008271|Serine/threonine protein kinase, active site (255 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 76 3e-16 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 63 3e-12 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 63 3e-12 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 63 3e-12 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 62 6e-12 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 57 1e-10 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 46 3e-07 DQ667194-1|ABG75746.1| 391|Apis mellifera cys-loop ligand-gated... 27 0.22 EF540769-1|ABQ14707.1| 620|Apis mellifera adenosine deaminase p... 24 1.6 EF493864-1|ABP65286.1| 247|Apis mellifera triosephoshpate isome... 22 4.7 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 76.2 bits (179), Expect = 3e-16 Identities = 65/243 (26%), Positives = 113/243 (46%), Gaps = 15/243 (6%) Query: 14 ASINDVSALFQNA--QKYEELSLIGTGAYGTVYKARDLHNGGQIVAMKKVKVA-LTEDGI 70 A+I +++ F++ Q L+ +G G +G V + + + A+K++K A + E Sbjct: 350 ATIPELNEEFRDLRLQDLRPLATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQ 409 Query: 71 PLSTVREIALLRQLEAYRHPNIVRLLDVCHGGQCLERDQQLVLFLVFEHVEQDLDSFLSR 130 + E ++ + + +V+L +D++ + L+ + +L + L R Sbjct: 410 QQHIMSEKRIMGEADC---DFVVKLFKTF-------KDRKYLYMLMEACLGGELWTVL-R 458 Query: 131 APGPLPESRIRSMSHDIISGVDFLHSHRIVHRDLKPHNLLVTVAGRVKLADFGLAKTYDV 190 G + R + ++ D+LHS I++RDLKP NLL+ G VKL DFG AK D Sbjct: 459 DKGHFDDGTTRFYTACVVEAFDYLHSRNIIYRDLKPENLLLDSQGYVKLVDFGFAKRLDH 518 Query: 191 EMKLTSVVVTLWYRPPEVLLGARYCSALDVWSAGCVLAQLHTRRPLLPGASDSDQLHRIF 250 K + T Y PEV+L + + D WS G ++ +L T P G D + + I Sbjct: 519 GRKTWTFCGTPEYVAPEVILNKGHDISADYWSLGVLMFELLTGTPPFTG-GDPMKTYNII 577 Query: 251 RSG 253 G Sbjct: 578 LKG 580 Score = 21.4 bits (43), Expect = 8.3 Identities = 28/146 (19%), Positives = 59/146 (40%), Gaps = 8/146 (5%) Query: 39 AYGTVYKARDLHNGGQIVAMKKVKVALTEDGIPLSTVREIALLRQLEAYRHPNIVRLLDV 98 A T+ + D+ G + M++ KV ++ DG LST+ +L +L + + Sbjct: 125 AGSTIIREGDV--GSIVYVMEEGKVEVSRDGKYLSTLAPGKVLGELAILYNCKRTATITA 182 Query: 99 CHGGQCLERDQQLVLFLVFE---HVEQDLDSFLSRAP--GPLPESRIRSMSHDIISGVDF 153 Q D+Q ++ + + FL P LPE + +S D++ + Sbjct: 183 ATDCQLWAIDRQCFQTIMMRTGLSRQAEYTDFLKSVPIFKNLPEETLIKIS-DVLEETFY 241 Query: 154 LHSHRIVHRDLKPHNLLVTVAGRVKL 179 + I+ + + + G+V++ Sbjct: 242 NNGDYIIRQGARGDTFFIISRGQVRV 267 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 62.9 bits (146), Expect = 3e-12 Identities = 38/93 (40%), Positives = 56/93 (60%), Gaps = 6/93 (6%) Query: 146 DIISGVDFLHSHRIVHRDLKPHNLLVTVAGRVKLADFGLAKTYDVEMKLTSVVVTLWYRP 205 D++ G+ +LHS +VHRD+K N+L+ + R KL DFG T +V M L S+V T + Sbjct: 705 DVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCIT-EV-MMLGSIVGTPVHMA 762 Query: 206 PEVLLGARYCSALDVWSAGCVLAQL---HTRRP 235 PE+L G Y S++DV++ G + L H R P Sbjct: 763 PELLSG-HYDSSVDVYAFGILFWYLCAGHVRLP 794 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 62.9 bits (146), Expect = 3e-12 Identities = 38/93 (40%), Positives = 56/93 (60%), Gaps = 6/93 (6%) Query: 146 DIISGVDFLHSHRIVHRDLKPHNLLVTVAGRVKLADFGLAKTYDVEMKLTSVVVTLWYRP 205 D++ G+ +LHS +VHRD+K N+L+ + R KL DFG T +V M L S+V T + Sbjct: 743 DVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCIT-EV-MMLGSIVGTPVHMA 800 Query: 206 PEVLLGARYCSALDVWSAGCVLAQL---HTRRP 235 PE+L G Y S++DV++ G + L H R P Sbjct: 801 PELLSG-HYDSSVDVYAFGILFWYLCAGHVRLP 832 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 62.9 bits (146), Expect = 3e-12 Identities = 59/203 (29%), Positives = 96/203 (47%), Gaps = 21/203 (10%) Query: 33 SLIGTGAYGTVYKAR-DLHNGGQI---VAMKKVKVALTEDGIPLSTVREIALLRQLEAYR 88 ++IG G +G V + + L G+ VA+K +K + D + E +++ Q E Sbjct: 637 AIIGGGEFGDVCRGKLKLPPDGRTEIDVAIKTLKPG-SADKARNDFLTEASIMGQFE--- 692 Query: 89 HPNIVRLLDVCHGGQCLERDQQLVLFLVFEHVEQ-DLDSFLSRAPGPLPESRIRSMSHDI 147 HPN++ L V + + ++ E +E LD+FL G ++ M I Sbjct: 693 HPNVIFLQGVV--------TKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRGI 744 Query: 148 ISGVDFLHSHRIVHRDLKPHNLLVTVAGRVKLADFGLAKTYD--VEMKLTS--VVVTLWY 203 SG+ +L VHRDL N+LV A K+ADFGL++ + E T+ + + + Sbjct: 745 ASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKIPVRW 804 Query: 204 RPPEVLLGARYCSALDVWSAGCV 226 PE + ++ SA DVWS G V Sbjct: 805 TAPEAIAFRKFTSASDVWSMGIV 827 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 61.7 bits (143), Expect = 6e-12 Identities = 57/203 (28%), Positives = 94/203 (46%), Gaps = 23/203 (11%) Query: 33 SLIGTGAYGTVYKARDLHNGGQIVAMKKVKVALTED-GIPLSTVREIALLRQLEAYRHPN 91 + +G+G +G VYKA L+ G Q+ A K+ TE L++ + + L+ H N Sbjct: 71 TFLGSGGFGIVYKA--LYKGEQVAA----KIIQTEKYSNMLNSEKHASFLK------HSN 118 Query: 92 IVRLLDVCHGGQCLERDQQLVLFLVFEHVEQDLDSFLSRAPGPLPESRIRSMSHDIISGV 151 IV++L + G + + L ++ LD + + RI + I + Sbjct: 119 IVKVLMIEQGASL----SLITMELCGTTLQNRLDEAIL-----IKNERICILK-SITCAL 168 Query: 152 DFLHSHRIVHRDLKPHNLLVTVAGRVKLADFGLAKTYDVEMKLTSVVVTLWYRPPEVLLG 211 F H+ IVH D+KP N+L++ G+ KL DFG + ++ T Y PEV+ Sbjct: 169 QFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAPEVIKQ 228 Query: 212 ARYCSALDVWSAGCVLAQLHTRR 234 R A D++S G V Q+ R+ Sbjct: 229 NRPTPAADIYSLGIVAWQMLFRK 251 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 57.2 bits (132), Expect = 1e-10 Identities = 41/108 (37%), Positives = 53/108 (49%), Gaps = 8/108 (7%) Query: 147 IISGVDFLHSHRIVHRDLKPHNLLVTVAGR---VKLADFGLAKTYDVEMKL-TSVVVTLW 202 I+ V H + +VHRDLKP NLL+ + VKLADFGLA E + T Sbjct: 18 ILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEAQAWFGFAGTPG 77 Query: 203 YRPPEVLLGARYCSALDVWSAGCVLAQLHTRRPLLPGASDSDQLHRIF 250 Y PEVL Y +D+W+ G +L L P D DQ HR++ Sbjct: 78 YLSPEVLKKEPYGKPVDIWACGVILYILLVG---YPPFWDEDQ-HRLY 121 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 46.4 bits (105), Expect = 3e-07 Identities = 26/74 (35%), Positives = 39/74 (52%) Query: 113 LFLVFEHVEQDLDSFLSRAPGPLPESRIRSMSHDIISGVDFLHSHRIVHRDLKPHNLLVT 172 L+ V E+V + + G E + +I G+ FLH IV+RDLK N+L+ Sbjct: 60 LYFVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLD 119 Query: 173 VAGRVKLADFGLAK 186 G +K+ADFG+ K Sbjct: 120 QDGHIKIADFGMCK 133 >DQ667194-1|ABG75746.1| 391|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 391 Score = 26.6 bits (56), Expect = 0.22 Identities = 9/14 (64%), Positives = 11/14 (78%) Query: 214 YCSALDVWSAGCVL 227 Y ALDVW AGC++ Sbjct: 258 YVKALDVWMAGCMM 271 >EF540769-1|ABQ14707.1| 620|Apis mellifera adenosine deaminase protein. Length = 620 Score = 23.8 bits (49), Expect = 1.6 Identities = 15/57 (26%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Query: 151 VDFLHSHRIVHRDL-KPHNLLVTVAGRVKLADFGLAKTYDVEMKLTSVVVTLWYRPP 206 ++ ++ H IV L P ++ V GR++ GL Y + L S++ + R P Sbjct: 456 IEPIYFHSIVLGSLLNPSHMYRAVCGRIENTIQGLPPPYRLNKPLMSLITSSEVRQP 512 >EF493864-1|ABP65286.1| 247|Apis mellifera triosephoshpate isomerase protein. Length = 247 Score = 22.2 bits (45), Expect = 4.7 Identities = 9/22 (40%), Positives = 14/22 (63%) Query: 29 YEELSLIGTGAYGTVYKARDLH 50 YE + IGTG T +A+++H Sbjct: 163 YEPVWAIGTGKTATPQQAQEVH 184 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.322 0.137 0.406 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 73,384 Number of Sequences: 429 Number of extensions: 2819 Number of successful extensions: 24 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 8 Number of HSP's gapped (non-prelim): 12 length of query: 255 length of database: 140,377 effective HSP length: 56 effective length of query: 199 effective length of database: 116,353 effective search space: 23154247 effective search space used: 23154247 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 43 (21.4 bits)
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