Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001343-TA|BGIBMGA001343-PA|IPR000608|Ubiquitin-
conjugating enzyme, E2
(201 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 62 6e-11
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 51 1e-07
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 47 2e-06
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 44 1e-05
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 41 1e-04
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 41 1e-04
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 39 4e-04
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 37 0.002
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 37 0.002
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 32 0.051
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 30 0.21
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 26 3.3
SPBP16F5.08c |||flavin dependent monooxygenase |Schizosaccharomy... 26 4.4
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 62.1 bits (144), Expect = 6e-11
Identities = 39/136 (28%), Positives = 74/136 (54%), Gaps = 4/136 (2%)
Query: 2 LQTENLRGIYVIPSYESSFLWYGVIFVRSGL-YEGGVFRFTITLPEKFPDDETPVLTFVS 60
+Q + G+ P ++ LW VI + +E G F+ ++ E++P+ + P++ FVS
Sbjct: 16 MQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPN-KPPLVKFVS 74
Query: 61 NLYHPAIDASTGLLSLREVFPMWDKKRDHVWQILKYLHIIFDNLSIKVPANTEAACMYKT 120
++HP + A+ G L L + W D V IL + + ++ + PAN EAA +++
Sbjct: 75 TMFHPNVYAN-GELCLDILQNRWSPTYD-VAAILTSIQSLLNDPNNASPANAEAAQLHRE 132
Query: 121 NRKFFMQKVRECVVTS 136
N+K ++++VR+ V S
Sbjct: 133 NKKEYVRRVRKTVEDS 148
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 51.2 bits (117), Expect = 1e-07
Identities = 32/99 (32%), Positives = 53/99 (53%), Gaps = 3/99 (3%)
Query: 33 YEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDAS-TGLLSLREVFPMWDKKRDHVW 91
+EGG+++ T+ PE++P P F L+HP + S T LS+ W K +
Sbjct: 53 WEGGLYKLTMAFPEEYPT-RPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGW-KPAITIK 110
Query: 92 QILKYLHIIFDNLSIKVPANTEAACMYKTNRKFFMQKVR 130
QIL + + D+ +I PA TEA M+K ++ + ++VR
Sbjct: 111 QILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEKRVR 149
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 47.2 bits (107), Expect = 2e-06
Identities = 26/101 (25%), Positives = 49/101 (48%), Gaps = 1/101 (0%)
Query: 33 YEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGLLSLREVFPMWDKKRDHVWQ 92
Y GG+++ + LP ++P ++P + FV+ ++HP ID +G + L + W D +
Sbjct: 44 YSGGIWKVHVELPSEYPW-KSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINI 102
Query: 93 ILKYLHIIFDNLSIKVPANTEAACMYKTNRKFFMQKVRECV 133
+L + + P N EAA + + KVR+ +
Sbjct: 103 FEVFLPQLLRYPNASDPLNGEAAALLLREPNTYYAKVRDYI 143
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 44.4 bits (100), Expect = 1e-05
Identities = 30/100 (30%), Positives = 51/100 (51%), Gaps = 3/100 (3%)
Query: 31 GLYEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGLLSLREVFPMWDKKRDHV 90
G Y+GG F+F I + + +P D P + ++ +YHP ID G + L + W+ ++
Sbjct: 63 GYYKGGKFKFRIQIDDNYPHD-PPKVKCLNKIYHPNIDIE-GNVCLNILRQDWNPVL-NL 119
Query: 91 WQILKYLHIIFDNLSIKVPANTEAACMYKTNRKFFMQKVR 130
IL L +F + + + P N EAA + + F +VR
Sbjct: 120 NSILVGLQFLFLSPNAEDPLNKEAAADLHKDPQGFASRVR 159
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 40.7 bits (91), Expect = 1e-04
Identities = 37/137 (27%), Positives = 65/137 (47%), Gaps = 15/137 (10%)
Query: 17 ESSFLWYGVIF-VRSGLYEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGLLS 75
+S F W +I LYEGG F T++ P+ +P P + F + ++HP + + G +
Sbjct: 33 KSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYP-LMPPKMKFTTEIWHPNVHPN-GEVC 90
Query: 76 LREVFPMWDKKRDHV-----W-------QILKYLHIIFDNLSIKVPANTEAACMYKTNRK 123
+ + P D K + W IL + + + + + PAN +AA ++ N +
Sbjct: 91 ISILHPPGDDKYGYEDAGERWLPVHSPETILISVISMLSSPNDESPANIDAAKEFRENPQ 150
Query: 124 FFMQKVRECVVTSIDHI 140
F ++VR V SI+ I
Sbjct: 151 EFKKRVRRLVRRSIEMI 167
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 40.7 bits (91), Expect = 1e-04
Identities = 32/119 (26%), Positives = 52/119 (43%), Gaps = 4/119 (3%)
Query: 14 PSYESSFLWYGVIF-VRSGLYEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTG 72
P + F W I Y GGVF +I P +P + P + F + +YHP I+ S G
Sbjct: 25 PVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYP-FKPPKVNFTTRIYHPNIN-SNG 82
Query: 73 LLSLREVFPMWDKKRDHVWQILKYLHIIFDNLSIKVPANTEAACMYKTNRKFFMQKVRE 131
+ L + W + ++L + + + + P E A +YKT+R + RE
Sbjct: 83 SICLDILRDQWSPALT-ISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELSARE 140
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 39.1 bits (87), Expect = 4e-04
Identities = 26/99 (26%), Positives = 43/99 (43%), Gaps = 2/99 (2%)
Query: 33 YEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGLLSLREVFPMWDKKRDHVWQ 92
YEGG F I +P +P P + F + +YHP + + TG + L + W +
Sbjct: 48 YEGGYFVVDIEIPIDYPF-RPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYT-MKS 105
Query: 93 ILKYLHIIFDNLSIKVPANTEAACMYKTNRKFFMQKVRE 131
L L + P + + A +Y N + F++ RE
Sbjct: 106 ALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTARE 144
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 37.1 bits (82), Expect = 0.002
Identities = 24/99 (24%), Positives = 45/99 (45%), Gaps = 3/99 (3%)
Query: 33 YEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGLLSLREVFPMWDKKRDHVWQ 92
YEGG F + LP+++P P + F++ +YHP +D G + L + W +
Sbjct: 46 YEGGKFHLELFLPDEYP-MMPPNVRFLTKIYHPNVD-KLGRICLSTLKKDWSPAL-QIRT 102
Query: 93 ILKYLHIIFDNLSIKVPANTEAACMYKTNRKFFMQKVRE 131
+L + + + P + + A ++K N + RE
Sbjct: 103 VLLSIQALMGAPNPDDPLDNDVAKIWKENEPQAIANARE 141
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 36.7 bits (81), Expect = 0.002
Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 4/69 (5%)
Query: 32 LYEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGL---LSLREVFPMWDKKRD 88
+Y GG F F++ P +P + P + F + +YHP D+ + + ++VF K R
Sbjct: 50 VYAGGKFHFSLKFPLDYP-FQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRS 108
Query: 89 HVWQILKYL 97
+ QIL+ L
Sbjct: 109 VLEQILQLL 117
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 32.3 bits (70), Expect = 0.051
Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Query: 32 LYEGGVFRFTITLPEKFPDDETPVLTFVSNLYHPAIDASTGLLSLREVFPMWDKKRDH 89
++E ++ I +PD P++TFVS + P +D TG ++ ++ + KR++
Sbjct: 52 VHENRIYSLKIHCDANYPD-APPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREY 108
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 30.3 bits (65), Expect = 0.21
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
Query: 137 IDHIYDDPPTEDK--HYITF-KPYDPDIHEKAKDIMLTAFKP 175
+D IY PP D+ H F K YD + ++KD +L+A+ P
Sbjct: 1375 LDEIYSVPPNLDRESHSRPFGKKYDTVVFNRSKDRLLSAYPP 1416
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 26.2 bits (55), Expect = 3.3
Identities = 17/57 (29%), Positives = 24/57 (42%), Gaps = 3/57 (5%)
Query: 30 SGLYEGGVFRFTITLPEKFPDDETPVLTFVSNL--YHPAIDASTGLLSLREVFPMWD 84
SG G+ + TI L + F T L + + HPAI + +EV WD
Sbjct: 40 SGALASGILKLTI-LHQPFIKVHTLKLQLIKRITVLHPAISHCSACAGSKEVLQTWD 95
>SPBP16F5.08c |||flavin dependent monooxygenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 447
Score = 25.8 bits (54), Expect = 4.4
Identities = 15/33 (45%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Query: 26 IFVRSGLYEGGVFRFTITLPEKFPDDET-PVLT 57
+F R G GGV+ +T TL K P T P+LT
Sbjct: 36 LFERRGS-PGGVWNYTSTLSNKLPVPSTNPILT 67
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.322 0.138 0.434
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,046,306
Number of Sequences: 5004
Number of extensions: 47022
Number of successful extensions: 112
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 100
Number of HSP's gapped (non-prelim): 13
length of query: 201
length of database: 2,362,478
effective HSP length: 69
effective length of query: 132
effective length of database: 2,017,202
effective search space: 266270664
effective search space used: 266270664
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 52 (25.0 bits)
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