Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001334-TA|BGIBMGA001334-PA|IPR001478|PDZ/DHR/GLGF,
IPR008145|Guanylate kinase/L-type calcium channel region,
IPR008144|Guanylate kinase
(567 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_59147| Best HMM Match : No HMM Matches (HMM E-Value=.) 243 3e-64
SB_48455| Best HMM Match : No HMM Matches (HMM E-Value=.) 128 1e-29
SB_17677| Best HMM Match : No HMM Matches (HMM E-Value=.) 99 5e-21
SB_33618| Best HMM Match : Guanylate_kin (HMM E-Value=6.3e-13) 93 4e-19
SB_44409| Best HMM Match : PDZ (HMM E-Value=7.1e-33) 91 2e-18
SB_37439| Best HMM Match : Guanylate_kin (HMM E-Value=2.3e-21) 91 2e-18
SB_45036| Best HMM Match : Guanylate_kin (HMM E-Value=0) 56 6e-08
SB_11360| Best HMM Match : PDZ (HMM E-Value=0) 50 7e-06
SB_15888| Best HMM Match : No HMM Matches (HMM E-Value=.) 45 1e-04
SB_26957| Best HMM Match : PDZ (HMM E-Value=0) 40 0.007
SB_31500| Best HMM Match : No HMM Matches (HMM E-Value=.) 38 0.016
SB_40268| Best HMM Match : NO_synthase (HMM E-Value=0) 38 0.016
SB_27031| Best HMM Match : No HMM Matches (HMM E-Value=.) 38 0.029
SB_3441| Best HMM Match : PDZ (HMM E-Value=0.0055) 38 0.029
SB_32768| Best HMM Match : No HMM Matches (HMM E-Value=.) 36 0.12
SB_7216| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.35
SB_4725| Best HMM Match : Mt_ATP-synt_D (HMM E-Value=1.4) 34 0.35
SB_31410| Best HMM Match : PDZ (HMM E-Value=1.1e-15) 33 0.47
SB_16761| Best HMM Match : PDZ (HMM E-Value=1.6e-08) 33 0.47
SB_8332| Best HMM Match : PDZ (HMM E-Value=1.3e-17) 33 0.47
SB_53824| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.61
SB_36803| Best HMM Match : RhoGAP (HMM E-Value=0) 33 0.61
SB_52290| Best HMM Match : PDZ (HMM E-Value=8.6e-18) 33 0.81
SB_3725| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.1
SB_29906| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.1
SB_47680| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.4
SB_9416| Best HMM Match : WD40 (HMM E-Value=0) 31 1.9
SB_32863| Best HMM Match : Exo_endo_phos (HMM E-Value=1.2e-06) 31 2.5
SB_4077| Best HMM Match : PDZ (HMM E-Value=3.5e-18) 31 2.5
SB_4655| Best HMM Match : Spectrin (HMM E-Value=0) 31 3.3
SB_1088| Best HMM Match : bZIP_1 (HMM E-Value=0.78) 31 3.3
SB_41041| Best HMM Match : PDZ (HMM E-Value=1.3e-40) 31 3.3
SB_53398| Best HMM Match : RVT_1 (HMM E-Value=7.8e-12) 30 4.3
SB_51557| Best HMM Match : Collagen (HMM E-Value=0.56) 30 4.3
SB_34765| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 4.3
SB_25368| Best HMM Match : PID (HMM E-Value=2.7e-22) 30 4.3
SB_49600| Best HMM Match : RVT_1 (HMM E-Value=0) 29 7.6
SB_49131| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 7.6
SB_59527| Best HMM Match : DUF382 (HMM E-Value=4.1e-26) 29 7.6
SB_25064| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 7.6
>SB_59147| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 574
Score = 243 bits (595), Expect = 3e-64
Identities = 170/540 (31%), Positives = 278/540 (51%), Gaps = 48/540 (8%)
Query: 47 LKSIVESPDFARKYGCADSEEDLLVKPVSLGNVNIIRSLSEYRGNNIRTIEQA-ELAIIV 105
LK+I+ P + D E+ K + +++ + E + + + A EL ++
Sbjct: 40 LKNILGDPSVLKLIRAHDRLEEGRKKVENDDTNKLVKQVLEELEPQVGSDDSAAELCGLL 99
Query: 106 SRAHFKALIEAHDQIG-KIWLER--GSGID--DKLIPNEFPDLIEFSKESPEGNG-DMPI 159
HF+AL++ HD + K ++ G+D + E ++ K + E G + +
Sbjct: 100 QEPHFRALMQTHDAVAFKTYMPTPPSPGVDSFNGFAAGEPIRMVGLHKHANEHLGVTLKV 159
Query: 160 ETVKVVGLRKVPGQPL---GLTVTTDEHGQLIVARILAGSAAAKQALV--SVGDVLLEVD 214
E +++ R + G + GL D + + + ++G+ + Q + S G + L+V
Sbjct: 160 EDNQLIISRIIHGGMIDRQGLLHVGDIIKE-VNGKDMSGNPSELQDYLAKSTGKITLKVL 218
Query: 215 GVHIESEEQLKEAVAKPNDRVTLKVGPNLKEKSSQLTNK----LTISDRKDPNWWQASHV 270
+ ES + V D K + + + L K L I D++D NWWQA V
Sbjct: 219 PSYYESPALCQVFVRAHFDYDPSK-DDQIPCQDAGLAFKQGEVLQIVDQEDANWWQAKKV 277
Query: 271 ERPEIVGLIPSPELEERRKAYVPPEADFVH-----KISICGARISRKKK---KFVYESRS 322
+ G+IPS EE VP F++ + +C +SR K KF +
Sbjct: 278 DDSRAAGIIPSQICEESEGVAVPSILMFLYFTPLGLLQLCLILVSRLIKVLFKFKQQFNE 337
Query: 323 SVQLEGAELALYEEVARTPPFLRRVLALVGTRGVGRRTLKNRLIQDYPDRFGAVVPHTSR 382
E EL +YEEVA+ PPF R+ L ++G +GVGRRTLKN+LI RFG +PHTSR
Sbjct: 338 ETDFEKHELPIYEEVAKMPPFQRKTLVMIGAQGVGRRTLKNKLIMSDRKRFGTTIPHTSR 397
Query: 383 PPRPMEENGQSYWFVSREEMERDAHAGRFLEYGEHNGNLYGTHLDSIRAVIKEGKMCILD 442
R E++G+ Y+FVSREEMERD AG++LE+GE+ GN+YGT +D+IR V++ GKMC++D
Sbjct: 398 QMREGEQSGRGYFFVSREEMERDIRAGKYLEFGEYGGNMYGTKIDTIREVMRSGKMCVVD 457
Query: 443 CAPQSLKLLHNSSEFLPYVVMITAPGIEQLKNL-THASNRNLTFDRQSSIRYSSRRARTL 501
P +LK+L + EF+PY+V I +P E+LK + A + + ++S
Sbjct: 458 VNPTALKVL-KTPEFMPYIVCIASPSFEELKEFQSKALSEGIVNKKRS------------ 504
Query: 502 ESLASLYEEEDLKQTLEESARIQRQYEKYIDFVITNNNSDTTFSKILDALHALSTDHQWV 561
+++L++T+E+S R+ Y Y D I N+N + F+K+ +A+ +LST QW+
Sbjct: 505 --------DDELRKTVEDSERLYNSYAHYFDLTIANDNVEEAFNKLQNAIESLSTHPQWI 556
>SB_48455| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 622
Score = 128 bits (309), Expect = 1e-29
Identities = 78/206 (37%), Positives = 113/206 (54%), Gaps = 23/206 (11%)
Query: 363 NRLIQDYPDRFGAVV-PHTSRPPRPMEENGQSYWFVSREEMERDAHAGRFLEYGEHNGNL 421
+R + Y DR+ A T+R PR EE+G+ Y+FVS E M D + R+LEYG H G +
Sbjct: 439 SRKKKTYRDRYVAKHNADTTRDPRAGEEDGKHYYFVSAEVMMSDIESNRYLEYGTHEGAM 498
Query: 422 YGTHLDSIRAVIKEGKMCILDCAPQSLKLLHNSSEFLPYVVMITAPGIEQLKNLTHASNR 481
YGT LD+I +I+ K+ ILD PQ+LK+L +++F PYVV I+AP I+ +
Sbjct: 499 YGTKLDTITDIIENHKVAILDVEPQALKIL-RTAKFTPYVVFISAPSIKGI--------- 548
Query: 482 NLTFDRQSSIRYSSRRARTLESLASLYEEEDLKQTLEESARIQRQYEKYIDFVITNNNSD 541
+ IR E E LK+ +ES ++++ Y D I NN+ D
Sbjct: 549 -------NDIRPPVANHEPAEDAG-----ESLKRLAKESDQLRQTYGHLFDLTIVNNDID 596
Query: 542 TTFSKILDALHALSTDHQWVPVSWIY 567
T +++ +AL L T QWVP+SWIY
Sbjct: 597 ETIAQLEEALERLGTTPQWVPISWIY 622
>SB_17677| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 685
Score = 99 bits (238), Expect = 5e-21
Identities = 49/95 (51%), Positives = 60/95 (63%)
Query: 352 GTRGVGRRTLKNRLIQDYPDRFGAVVPHTSRPPRPMEENGQSYWFVSREEMERDAHAGRF 411
G GVGR LK RL+ + + VPHTSR RP E NG+ Y+FVSR ME A +F
Sbjct: 452 GPPGVGRNELKKRLLACDNELYTTAVPHTSRRMRPYEANGREYYFVSRTTMENYAQNSKF 511
Query: 412 LEYGEHNGNLYGTHLDSIRAVIKEGKMCILDCAPQ 446
+EYGE LYGT +DSI+ VI+ GKMCIL P+
Sbjct: 512 IEYGEFRKCLYGTSVDSIKRVIRTGKMCILKVQPK 546
Score = 58.8 bits (136), Expect = 1e-08
Identities = 55/204 (26%), Positives = 101/204 (49%), Gaps = 13/204 (6%)
Query: 46 FLKSIVESPDFAR--KYGCADSEEDLLVKPVSLGNVN-IIRSLSEYRGNNIRTIEQA-EL 101
FL S S +F K + S +D+ + N + + + + GN + E+A EL
Sbjct: 70 FLSSYFHSQEFKNLFKVHTSVSTKDIYRNTIERENASEMATQVLDTLGNLAQPDEEACEL 129
Query: 102 AIIVSRAHFKALIEAHDQIGKIWLERGSGIDDKLIPNEFPDLIEFSKESPEGNGDMPIE- 160
A ++S H + ++ A+D++ +I + + + +I D + ++ +G+ D +E
Sbjct: 130 AQLLSNVHIQGMLSAYDRVAEIDVSQQNQESGTVIG----DGGQRNENGYKGS-DSDLEL 184
Query: 161 TVKVVGLRKVPGQPLGLTVTTDEH-GQLIVARILAGSAAAKQALVSVGDVLLEVDGVHI- 218
VK V L K +PLG TV DE G ++VARIL G + + VGDV+ E++ +
Sbjct: 185 AVKYVRLIKSGKEPLGATVKCDEDTGDIVVARILRGGMVDRSGTLGVGDVIQEINNQSVI 244
Query: 219 -ESEEQLKEAVAKPNDRVTLKVGP 241
++ ++ E + + + V KV P
Sbjct: 245 GKTTNEVVEIMERLSGSVLFKVIP 268
Score = 47.2 bits (107), Expect = 4e-05
Identities = 35/112 (31%), Positives = 52/112 (46%), Gaps = 11/112 (9%)
Query: 254 LTISDRKDPNWWQA-SHVERPEIVGLIPSPELEERRKAYVPPEADFV-HKISICGAR--- 308
L + R+DPNWWQA + +GL+PS + +E+R + A+ HK S G
Sbjct: 313 LQVVSREDPNWWQARKEDDVNNTIGLVPSKQFQEKRFLFDRLFAETENHKHSSGGVNHVK 372
Query: 309 ----ISRKKKKFVYESRSSVQLEGAELALYEEVARTPPF--LRRVLALVGTR 354
RKKK+ + +G + YEEV P R++ L+GTR
Sbjct: 373 NFRWRKRKKKEHKLSDAQKDETDGDKALTYEEVTMFSPVNDKHRLVILIGTR 424
>SB_33618| Best HMM Match : Guanylate_kin (HMM E-Value=6.3e-13)
Length = 171
Score = 93.5 bits (222), Expect = 4e-19
Identities = 57/187 (30%), Positives = 92/187 (49%), Gaps = 18/187 (9%)
Query: 380 TSRPPRPMEENGQSYWFVSREEMERDAHAGRFLEYGEHNGNLYGTHLDSIRAVIKEGKMC 439
TSRP +P E N Y FV R D A +F+E+GE + YGT D+I+ VI GK C
Sbjct: 2 TSRPIKPGEINDHDYHFVPRHTFVTDIVAHKFVEHGELQDHFYGTSFDAIKMVINAGKTC 61
Query: 440 ILDCAPQSLKLLHNSSEFLPYVVMITAPGIEQLKNLTHASNRNLTFDRQSSIRYSSRRAR 499
IL+ LK F PYV+ I++P E+++ L + F+ + ++ + R
Sbjct: 62 ILNLHCH-LKTSSCHQTFKPYVIFISSPSYERVRALRR--GKIDPFNPKLNVALTDR--- 115
Query: 500 TLESLASLYEEEDLKQTLEESARIQRQYEKYIDFVITNNNSDTTFSKILDALHALSTDHQ 559
++ + + ++ ++ Y Y D ITN + + TF ++ + L + Q
Sbjct: 116 ------------EIHEMVAKAREMEEAYGHYFDHTITNTDFERTFDELRQVIARLEDEPQ 163
Query: 560 WVPVSWI 566
WVPVSW+
Sbjct: 164 WVPVSWL 170
>SB_44409| Best HMM Match : PDZ (HMM E-Value=7.1e-33)
Length = 718
Score = 91.1 bits (216), Expect = 2e-18
Identities = 43/94 (45%), Positives = 57/94 (60%)
Query: 349 ALVGTRGVGRRTLKNRLIQDYPDRFGAVVPHTSRPPRPMEENGQSYWFVSREEMERDAHA 408
++ G +GR+ L+ RLIQ PDRF +PHTSRP +P E N Y FV R D A
Sbjct: 590 SVTGPPKIGRKELRQRLIQYDPDRFAGAIPHTSRPIKPGEINDHDYHFVPRHTFVTDIVA 649
Query: 409 GRFLEYGEHNGNLYGTHLDSIRAVIKEGKMCILD 442
+F+E+GE + YGT D+I+ VI GK CIL+
Sbjct: 650 HKFVEHGELQDHFYGTSFDAIKMVINAGKTCILN 683
Score = 64.9 bits (151), Expect = 2e-10
Identities = 46/152 (30%), Positives = 80/152 (52%), Gaps = 9/152 (5%)
Query: 95 TIEQAELAIIVSRAHFKALIEAHDQIGKIWLERGSGIDDKLIPNEFPDLIEFSKESPEGN 154
T ++ ELA ++S + L+ AHD+ L + D+ NE +L + + +
Sbjct: 383 TRDEVELASLLSSPTMQGLLLAHDR-----LAFREALPDENEENEGRELSRQNSSDADQS 437
Query: 155 GDMPIETVKVVGLRKVPGQPLGLTVTTDEHGQLIVARILAGSAAAKQALVSVGDVLLEVD 214
M ++VK+V + K PLG TV +E G +++ RI+ G AA K L+ GD +LE++
Sbjct: 438 SQMEDDSVKIVRIDK-SCDPLGATVK-NEDGAVLIGRIVKGGAAEKSGLLHEGDEILEIN 495
Query: 215 GVHI--ESEEQLKEAVAKPNDRVTLKVGPNLK 244
GVH+ +S ++ E +A N +T + P +
Sbjct: 496 GVHMKGKSVSEVCELLADMNGTLTFLLVPKTR 527
>SB_37439| Best HMM Match : Guanylate_kin (HMM E-Value=2.3e-21)
Length = 609
Score = 91.1 bits (216), Expect = 2e-18
Identities = 46/118 (38%), Positives = 68/118 (57%), Gaps = 1/118 (0%)
Query: 347 VLALVGTRGVGRRTLKNRLIQDYPDRFGAVVPHTSRPPRPMEENGQSYWFVSREEMERDA 406
+L L G G G+R L RL Q++PD FG V HT+RPPR EE+GQ Y+FV++EE E
Sbjct: 323 MLILCGPSGAGKRHLARRLCQEFPDFFGIGVSHTTRPPRKKEEHGQDYFFVNKEEFEHGV 382
Query: 407 HAGRFLEYGEHNGNLYGTHLDSIRAVIKEGKMCILDCAPQSLKLLHNSSEFLPYVVMI 464
+G FLE + G LYG +++ V +EG + + + L ++ F P V++
Sbjct: 383 MSGLFLETCQVGGFLYGVTREAVECVAREGLAAVFTMELEGV-LSFKNTYFEPRYVLV 439
>SB_45036| Best HMM Match : Guanylate_kin (HMM E-Value=0)
Length = 288
Score = 56.4 bits (130), Expect = 6e-08
Identities = 46/124 (37%), Positives = 61/124 (49%), Gaps = 12/124 (9%)
Query: 355 GVGRRTL-KNRLIQDYPDRFGAVVPHTSRPPRPMEENGQSYWFVSREEMERDAHAGRFLE 413
G G+ +L K L QD ++ V HT+R RP EE+G Y FVSRE + AG FLE
Sbjct: 15 GAGKTSLVKALLAQD--EQIRVSVSHTTRAMRPGEEDGVHYNFVSREAFDSLITAGNFLE 72
Query: 414 YGEHNGNLYGTHLDSIRAVIKEGKMCILDC----APQSLKLLHNSSEFLPYVVMITAPGI 469
Y E N YGT + + +G IL+ A Q KL+ N+ V I P
Sbjct: 73 YAEVFTNKYGTSRIWVEEQLAQGTDVILEIDWQGAQQVRKLMPNAVS-----VFILPPSK 127
Query: 470 EQLK 473
E+L+
Sbjct: 128 EELR 131
>SB_11360| Best HMM Match : PDZ (HMM E-Value=0)
Length = 625
Score = 49.6 bits (113), Expect = 7e-06
Identities = 31/82 (37%), Positives = 47/82 (57%), Gaps = 6/82 (7%)
Query: 319 ESRSSVQLEGAELALYEEVARTP-PFLRRVLALVGTRGVGRRTLKNRLIQDYPDRFGAVV 377
ES +S + E L+ YE V + + R V+ L G + + + LI ++PD+FG+ V
Sbjct: 431 ESSTSARAEEPILS-YEAVQQHELKYTRPVIVL----GPLKDRINDDLISEFPDKFGSCV 485
Query: 378 PHTSRPPRPMEENGQSYWFVSR 399
PHT+RP R E++G Y FV R
Sbjct: 486 PHTTRPRRDYEKDGLDYHFVDR 507
>SB_15888| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2437
Score = 45.2 bits (102), Expect = 1e-04
Identities = 26/84 (30%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Query: 141 PDLIEFSKESPEGNGDMPIETVKVVGLRKVPGQPLGLTVTTDEHGQ-----LIVARILAG 195
PD + + + P P ++V+ LRK PG+ LG T+ E+ Q + V + G
Sbjct: 945 PDTPQSTNQRPNDIVASPERDIQVIKLRKEPGKGLGFTILGGENSQRLDLGIFVKSVTEG 1004
Query: 196 SAAAKQALVSVGDVLLEVDGVHIE 219
S A K + GD +L V+G +E
Sbjct: 1005 SVAFKDGRLKAGDRILAVNGTSLE 1028
Score = 33.5 bits (73), Expect = 0.47
Identities = 29/106 (27%), Positives = 52/106 (49%), Gaps = 10/106 (9%)
Query: 164 VVGLRKVPGQPLGLTV-----TTDEHGQLIVARILAGSAAAKQALVSVGDVLLEVDGVHI 218
VV L K+ G LGL+V TT + G + V ++ A + + +GD +LEV+GV +
Sbjct: 1254 VVELEKI-GSSLGLSVSGGVNTTVKLGGIYVKSLMLNGPADRNGKIRIGDRILEVNGVSL 1312
Query: 219 --ESEEQLKEAVAKPNDRVTLKVGPNLKEKSSQLTNKLTISDRKDP 262
+ +Q E + + R L + +++ + IS++ DP
Sbjct: 1313 VGVTHKQAVEIIKRAPQRCRLVIDRSIQVNVPVPMKR--ISNKDDP 1356
Score = 30.3 bits (65), Expect = 4.3
Identities = 23/73 (31%), Positives = 36/73 (49%), Gaps = 10/73 (13%)
Query: 147 SKESPEGNGDMPIETVKVVGLRKVPGQPLGLTVTTD---EHGQLI-VARILAGSAAAKQA 202
+K+ PEG ++ VK +G G L L D EHG L+ + ++ G A
Sbjct: 1352 NKDDPEGQ-TFTVDLVKGIG-----GLGLSLVGGRDSDPEHGGLLRIKKMFPGQPATLSG 1405
Query: 203 LVSVGDVLLEVDG 215
+ VGD++ EV+G
Sbjct: 1406 KLQVGDIIQEVNG 1418
>SB_26957| Best HMM Match : PDZ (HMM E-Value=0)
Length = 1685
Score = 39.5 bits (88), Expect = 0.007
Identities = 31/105 (29%), Positives = 55/105 (52%), Gaps = 10/105 (9%)
Query: 156 DMP-IET---VKVVGLRKVPGQPLGLTVTTDEHGQ---LIVARILAGSAAAKQALVSVGD 208
D+P +ET ++VV LR+ P LG+++ ++ GQ + V +L S+ A+ + GD
Sbjct: 1282 DVPFVETESGLRVVELRREPEVGLGISIAGNKRGQRQGVHVRHVLENSSVARLGELKAGD 1341
Query: 209 VLLEVDGVHIE--SEEQLKEAVAKPNDRVTLKVGPNLKEKSSQLT 251
+LEVDG + S E+ E + + V V +++K L+
Sbjct: 1342 QILEVDGHDLRNASHEEAVEVIRRARSPVRFVV-RTIQDKEDPLS 1385
>SB_31500| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1446
Score = 38.3 bits (85), Expect = 0.016
Identities = 28/93 (30%), Positives = 48/93 (51%), Gaps = 7/93 (7%)
Query: 162 VKVVGLRKVPGQPLGLTVTTDEHGQLIVARILAGSAAAKQALVSVGDVLLEVDGVHIESE 221
V+ V L G+ LG+ + + ++ I GS AA ++++GD ++EV+G +E+
Sbjct: 794 VQPVRLTLSDGKELGIHIDNSK----FISFISPGSTAALNPMLAIGDKIVEVNGQSVENL 849
Query: 222 EQLKEAVAKPNDRVTLKVGPNLKEKSSQLTNKL 254
QL+ V+L V LK KS+ LT +
Sbjct: 850 SQLETEKLLCTSTVSLAV---LKPKSAHLTQAM 879
>SB_40268| Best HMM Match : NO_synthase (HMM E-Value=0)
Length = 465
Score = 38.3 bits (85), Expect = 0.016
Identities = 24/75 (32%), Positives = 41/75 (54%), Gaps = 6/75 (8%)
Query: 189 VARILAGSAAAKQALVSVGDVLLEVDGVHIESEEQLKEAV-----AKPNDRVTLKVGPNL 243
++ I+ GSAA + + GD++LEV+G IE E K+AV + V+LK+ P++
Sbjct: 33 ISDIVKGSAAEGVSNLQPGDIILEVNGKSIE-ESHFKDAVDVLSGVETGSSVSLKIKPSV 91
Query: 244 KEKSSQLTNKLTISD 258
+ S T+ I +
Sbjct: 92 RSNGSAKTSSKNIKE 106
>SB_27031| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 537
Score = 37.5 bits (83), Expect = 0.029
Identities = 36/124 (29%), Positives = 60/124 (48%), Gaps = 14/124 (11%)
Query: 127 RGSGI-DDKLIPNEFPDLIEFSKESPEGNGDMPIET-VKVVGLRKVPGQPLGLTVTTDEH 184
+ +GI +D + E+ ++E K E + ET + V + K PG+ LG+ +
Sbjct: 197 KSNGISEDSVEDAEYNCVLEAQKILGEDLSLLSDETKAREVVVNKKPGEALGVMIVESGW 256
Query: 185 GQLIVARILAGSA----AAKQALVSVGDVLLEVDGVHI------ESEEQLKEAVAKPNDR 234
G +I I+A A AAK V+VGD +L V+ + E + +K ++P +
Sbjct: 257 GSMIPTAIIAHLAKDGPAAKSGRVNVGDQILSVNNTSLVGLPLPECQNVIKN--SRPGTK 314
Query: 235 VTLK 238
VTLK
Sbjct: 315 VTLK 318
>SB_3441| Best HMM Match : PDZ (HMM E-Value=0.0055)
Length = 160
Score = 37.5 bits (83), Expect = 0.029
Identities = 28/92 (30%), Positives = 50/92 (54%), Gaps = 10/92 (10%)
Query: 112 ALIEAHDQIGKIWLERGSGIDDKLIPNEFP-DLIEFS-KESPEGNGDMPIETVKVVGLRK 169
A+++AHD + + + + I L P+ P D I+ S E PE + V++V K
Sbjct: 1 AMLQAHDGVLQEEKQDETSI---LPPDTTPNDTIDNSIPERPE-----KLTRVRLVQFHK 52
Query: 170 VPGQPLGLTVTTDEHGQLIVARILAGSAAAKQ 201
P +P+G+T+ ++ G+ +VARI+ G +Q
Sbjct: 53 NPNEPMGITLKLNDEGRCVVARIMYGGMIHRQ 84
>SB_32768| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1393
Score = 35.5 bits (78), Expect = 0.12
Identities = 19/48 (39%), Positives = 25/48 (52%)
Query: 377 VPHTSRPPRPMEENGQSYWFVSREEMERDAHAGRFLEYGEHNGNLYGT 424
VP T+R PR E G Y F++ ++ +G LE G GN YGT
Sbjct: 190 VPCTTRSPRNGEIPGVDYVFLNMQQFMDMEKSGALLESGVFEGNHYGT 237
>SB_7216| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1400
Score = 33.9 bits (74), Expect = 0.35
Identities = 22/67 (32%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Query: 175 LGLTVTTD---EHGQLIVARILAGSAAAKQALVSVGDVLLEVDGVHIESEEQLKEAVAKP 231
+GL V TD E + V + G AA K + V D ++EVDGV + Q+ AV
Sbjct: 661 IGLGVGTDTGVEKLGIFVKSLTEGGAAEKDGRIQVNDQIIEVDGVSLVGVTQMFAAVTLK 720
Query: 232 NDRVTLK 238
+ T++
Sbjct: 721 HTSGTVR 727
>SB_4725| Best HMM Match : Mt_ATP-synt_D (HMM E-Value=1.4)
Length = 465
Score = 33.9 bits (74), Expect = 0.35
Identities = 14/61 (22%), Positives = 33/61 (54%)
Query: 472 LKNLTHASNRNLTFDRQSSIRYSSRRARTLESLASLYEEEDLKQTLEESARIQRQYEKYI 531
L L HA N + D +S ++ + R++ + E+++K ++++S +QR + Y+
Sbjct: 220 LLTLLHAKNLEIIGDYRSFVQSKVNQGRSMALRMDQFREQEIKSSVDQSNDVQRSCDYYL 279
Query: 532 D 532
+
Sbjct: 280 E 280
>SB_31410| Best HMM Match : PDZ (HMM E-Value=1.1e-15)
Length = 556
Score = 33.5 bits (73), Expect = 0.47
Identities = 27/73 (36%), Positives = 41/73 (56%), Gaps = 6/73 (8%)
Query: 154 NGDMPIETVKVVGLRKV-PGQPLGLTVTTD-EHG-QLIVARILAGSAAAKQALVSVGDVL 210
NGD + KVV L K P Q LG ++ EHG + ++++ + S A KQ L +GD +
Sbjct: 121 NGDESM--FKVVTLVKTDPDQVLGFSIRGGREHGCGVYISQVDSDSQAEKQGL-HLGDQI 177
Query: 211 LEVDGVHIESEEQ 223
+EV+G+ E Q
Sbjct: 178 IEVNGIDFEQIAQ 190
>SB_16761| Best HMM Match : PDZ (HMM E-Value=1.6e-08)
Length = 889
Score = 33.5 bits (73), Expect = 0.47
Identities = 16/72 (22%), Positives = 38/72 (52%), Gaps = 2/72 (2%)
Query: 187 LIVARILAGSAAAKQALVSVGDVLLEVDGVHIES--EEQLKEAVAKPNDRVTLKVGPNLK 244
L V I G A++Q + +GD +L+++G H++ +++ + K D++ + ++
Sbjct: 503 LYVKDISFGGVASEQKSLEIGDYILKMNGKHLDKIIASSIEKVIKKSGDKMKFHITRMIQ 562
Query: 245 EKSSQLTNKLTI 256
S T+K+ +
Sbjct: 563 ASSRHGTSKVVL 574
>SB_8332| Best HMM Match : PDZ (HMM E-Value=1.3e-17)
Length = 1038
Score = 33.5 bits (73), Expect = 0.47
Identities = 25/72 (34%), Positives = 38/72 (52%), Gaps = 6/72 (8%)
Query: 147 SKESPEGNGDMPIETVKVVGLRKVPGQPLGLTVTTDEHGQL---IVARILAGSAAAKQAL 203
++E +GNGD + T++++ K LGL + L V+ IL S A+K
Sbjct: 107 NEEIEKGNGDEGVLTLEMI---KPSNGYLGLVLVGGADSPLKNHYVSDILPNSCASKCDC 163
Query: 204 VSVGDVLLEVDG 215
V +GD LLEV+G
Sbjct: 164 VRIGDELLEVNG 175
>SB_53824| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 740
Score = 33.1 bits (72), Expect = 0.61
Identities = 19/50 (38%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Query: 170 VPGQP-LGLTVTTDEHGQLIVARILAGSAAAKQALVSVGDVLLEVDGVHI 218
V QP LGLT+T + G V RI GS A K + +GD + ++G ++
Sbjct: 277 VKDQPALGLTITDNGAGCAFVKRIKEGSIADKTPQICIGDHVEYINGQYV 326
>SB_36803| Best HMM Match : RhoGAP (HMM E-Value=0)
Length = 1277
Score = 33.1 bits (72), Expect = 0.61
Identities = 29/110 (26%), Positives = 50/110 (45%), Gaps = 7/110 (6%)
Query: 146 FSKESPEGNGDMPIETVKVVGLRKVPGQPLGLTVTT-DEHGQ---LIVARILAGSAAAKQ 201
F +E+ + + + V +++ QPLG + D G+ + V+RI GS
Sbjct: 95 FLRENDSSSDISFTQGTRAVEIKRTHNQPLGFFIRQGDGWGRKDGVFVSRITPGSLVDCD 154
Query: 202 ALVSVGDVLLEVDGVHIES--EEQLKEAVAKPNDRVTLKVGPNLKEKSSQ 249
++VGD +LEV+GV + + + P R+ L + K K SQ
Sbjct: 155 GSLAVGDEILEVNGVCLAGFRVHDVVHLIQIPK-RLILTINKEAKVKRSQ 203
>SB_52290| Best HMM Match : PDZ (HMM E-Value=8.6e-18)
Length = 124
Score = 32.7 bits (71), Expect = 0.81
Identities = 20/56 (35%), Positives = 34/56 (60%), Gaps = 2/56 (3%)
Query: 165 VGLRKVPGQPLGLTVTTDEHG-QLIVARILAGSAAAKQALVSVGDVLLEVDGVHIE 219
V L K P Q LG+++T E G + + R++ G +AA + V DV+L+V+G ++
Sbjct: 11 VHLIKGP-QGLGMSLTGGESGGPIYIKRLVPGGSAALCGQLQVNDVILQVNGKSLD 65
>SB_3725| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1126
Score = 32.3 bits (70), Expect = 1.1
Identities = 28/131 (21%), Positives = 64/131 (48%), Gaps = 11/131 (8%)
Query: 149 ESPEGNGDMPIETVKVVGLRKVPGQPLGLTVTTDEHGQLIVARILAGSAAAKQALVSVGD 208
E P+ G+ I T++V+ ++V G + + + +++++I G AA + + +GD
Sbjct: 281 EPPDSIGNQ-IRTIRVIK-QEVGGLGISIKGGKENRMPILISKIFKGLAADQTESLYIGD 338
Query: 209 VLLEVDG------VHIESEEQLKEAVAKPNDRVT-LKVGPNLKEKSSQLTNKLTISDRKD 261
+L V+G H E+ LK A + + V +K + +++++ N+ + + D
Sbjct: 339 AILAVNGEDLRHATHDEAVRALKRAGKEVDLTVRHVKEVTSYFHRNAEMDNEQSTEGQGD 398
Query: 262 --PNWWQASHV 270
P W + ++
Sbjct: 399 NKPEWGEVKNI 409
>SB_29906| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2463
Score = 32.3 bits (70), Expect = 1.1
Identities = 56/202 (27%), Positives = 86/202 (42%), Gaps = 21/202 (10%)
Query: 207 GDVLLEVDGVHIE--SEEQLKEAVAKPNDRVTLKVGP--NLKEKSSQLTNKLTISDRKDP 262
GD L+EV+G ++E E++ E +A + V +KV P L E S + + D
Sbjct: 222 GDRLIEVNGTNVEDIDREKIIEMIAMSGEEVVIKVLPVSELSELSVRSGLDGSFIQLDDS 281
Query: 263 NWWQASHVERPEIVGLIPSPE-LEE--RRKAYVPPEADF-VHKISICGARISR-KKKKFV 317
N +A ++R + P+ +EE KA+ E + VHK G R+ R K +
Sbjct: 282 N-LKAGTLQRSGSKRMKKKPKSVEEVASNKAWHDAEKVWVVHKAGFSGGRVLRLKPSEGA 340
Query: 318 YESR---SSVQLE-GAELALYEE---VARTPPFLRRVLALVGTRGVGRRTLKNRLIQDYP 370
E V+L+ G EL EE PP R L R + + + L Q
Sbjct: 341 NEDEMPMCQVKLDHGGELITIEEENIEKANPPQYDRAEDLATLRYLNESSCLHTLRQ--- 397
Query: 371 DRFGAVVPHTSRPPRPMEENGQ 392
R+G+ + HT P + N Q
Sbjct: 398 -RYGSNLIHTYAGPNMIVINPQ 418
>SB_47680| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2749
Score = 31.9 bits (69), Expect = 1.4
Identities = 28/99 (28%), Positives = 44/99 (44%), Gaps = 5/99 (5%)
Query: 230 KPNDRVTLKVGPNLKEKS-SQLTNKLTISDRKDPN-WWQASHVE-RPEIVGLIPSPELEE 286
KP + LKV KS + T+ + + D N W H + + L+ SP+L
Sbjct: 933 KPKTKSELKVKSKPAHKSHKKQTSPIKMFSADDANNTWGTPHKDSKKRSPILLASPKL-- 990
Query: 287 RRKAYVPPEADFVHKISICGARISRKKKKFVYESRSSVQ 325
R+K + PP D V ++ + K K + ES+ S Q
Sbjct: 991 RKKHHSPPSPDLVFDTTLPSTPLPPKALKVLGESQPSTQ 1029
Score = 31.5 bits (68), Expect = 1.9
Identities = 18/76 (23%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Query: 175 LGLTVTTDEHGQLIVARILAGSAAAKQALVSVGDVLLEVDGVHIESE-----EQLKEAVA 229
LG+ ++ G+++V + + K V VGD LL ++ + ++++ QL +
Sbjct: 507 LGIKAGLNDSGRVVVTEVSSFGLVGKDGNVRVGDYLLSINDIKLDNKSAEEVNQLLKTCP 566
Query: 230 KPNDRVTLKVGPNLKE 245
K V K GP + +
Sbjct: 567 KGIVAVEAKAGPEVSQ 582
>SB_9416| Best HMM Match : WD40 (HMM E-Value=0)
Length = 700
Score = 31.5 bits (68), Expect = 1.9
Identities = 19/51 (37%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
Query: 380 TSRPPRPMEENGQSYWFVSREEMERDAHAG-RFLEYGEHNGNLYGTHLDSI 429
T R P S FV R+E E+ +G L+Y + NG LY DSI
Sbjct: 3 TRRSPNQPHRRKVSVSFVIRDEEEKRHRSGINALQYDKSNGRLYSAGRDSI 53
>SB_32863| Best HMM Match : Exo_endo_phos (HMM E-Value=1.2e-06)
Length = 309
Score = 31.1 bits (67), Expect = 2.5
Identities = 16/53 (30%), Positives = 26/53 (49%)
Query: 512 DLKQTLEESARIQRQYEKYIDFVITNNNSDTTFSKILDALHALSTDHQWVPVS 564
+L Q + ++ RI E ID +ITN T+ +L LH D ++ V+
Sbjct: 220 NLTQHVTKATRITSHSETLIDHIITNMPHRVTYCDVLPGLHVSDHDAPYIYVN 272
>SB_4077| Best HMM Match : PDZ (HMM E-Value=3.5e-18)
Length = 458
Score = 31.1 bits (67), Expect = 2.5
Identities = 18/59 (30%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
Query: 163 KVVGLRKVPGQPLGLTVTTD-EHG-QLIVARILAGSAAAKQALVSVGDVLLEVDGVHIE 219
+ V + K + LG+++T EHG ++++ I G AA+ + VGD +L V+G+ ++
Sbjct: 245 RTVIVNKDKTEGLGISITGGKEHGVPILISEIHDGMPAARCGGLYVGDAILAVNGIDLQ 303
>SB_4655| Best HMM Match : Spectrin (HMM E-Value=0)
Length = 934
Score = 30.7 bits (66), Expect = 3.3
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Query: 470 EQLKNLTHASNRNLTFDRQSSIRYSSRRARTLESLASLYEEEDLKQ-TLEESARIQRQYE 528
E+ KN+ +NR + D + + RR L ++E + ++ LEES ++Q Q+E
Sbjct: 486 EKFKNIDDFANRLIDGDHYAKDEVAERRDAVLNRRGKIHEASEARRLKLEESRKLQ-QFE 544
Query: 529 KYIDFV 534
+ D V
Sbjct: 545 RDADEV 550
>SB_1088| Best HMM Match : bZIP_1 (HMM E-Value=0.78)
Length = 398
Score = 30.7 bits (66), Expect = 3.3
Identities = 30/129 (23%), Positives = 57/129 (44%), Gaps = 11/129 (8%)
Query: 393 SYWFVSREEMERDAHAGRFLEYGEHNGNLYGTHLDSIRAVIKE-----GKMCILDCAPQS 447
S W S EEM AG+ G+H+ +L G I ++K + L+ +
Sbjct: 59 SSWMKSAEEM---GTAGKKTRLGKHDNSLEGERFKFIDKMLKNYEYYRKRESYLEKLQKK 115
Query: 448 LKLLHNSSEFLPYVVM--ITAPGIEQLKNLTHASNRNLTFDRQSSIRYSSRR-ARTLESL 504
L+ + L ++ + GI++L N THA + D Q++++ ++ L+
Sbjct: 116 HAQLNKQHDHLQKLLKAKLHGKGIKELHNTTHAKVNESSKDLQTTVKALKKKLVDLLKRQ 175
Query: 505 ASLYEEEDL 513
L +++DL
Sbjct: 176 KKLKKQQDL 184
>SB_41041| Best HMM Match : PDZ (HMM E-Value=1.3e-40)
Length = 933
Score = 30.7 bits (66), Expect = 3.3
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 5/57 (8%)
Query: 172 GQPLGLTVTTDE-----HGQLIVARILAGSAAAKQALVSVGDVLLEVDGVHIESEEQ 223
G LG ++TT + H + V ILA AA + + GD ++EV+G+ + + Q
Sbjct: 178 GDGLGFSITTRDNPAGGHTPIFVKSILAKGAAIEDGRLRGGDQIIEVNGMPMTGKNQ 234
>SB_53398| Best HMM Match : RVT_1 (HMM E-Value=7.8e-12)
Length = 924
Score = 30.3 bits (65), Expect = 4.3
Identities = 37/128 (28%), Positives = 55/128 (42%), Gaps = 9/128 (7%)
Query: 189 VARILAGSAAAKQALVSVGDVLLEVDG-VHIESEEQLKEAVAKPNDRVTLKVGPNLKEKS 247
V+ I G A V + L VDG HI + + + P +V L + P LKE
Sbjct: 357 VSEISKGDAEILNEFNDVFEGLGLVDGEYHITLSNEAQPTIHPPR-KVPLSLMPKLKETL 415
Query: 248 SQLTNKLTISDRKDPNWWQASHV---ERPEIVGLIPSP-ELEE--RRKAYVPPEADFVHK 301
+LT IS + W +S V ++ + L P EL +R+ Y PP A+ +
Sbjct: 416 EKLTRMGVISKVEKATDWVSSLVIVEKKNGTLRLCLDPKELNNAIKREHYKPPTAETLSS 475
Query: 302 ISICGARI 309
+ G RI
Sbjct: 476 -KLSGKRI 482
>SB_51557| Best HMM Match : Collagen (HMM E-Value=0.56)
Length = 697
Score = 30.3 bits (65), Expect = 4.3
Identities = 23/103 (22%), Positives = 49/103 (47%), Gaps = 3/103 (2%)
Query: 449 KLLHNSSEFLPYVVMITAPGIEQLKNLTHASNRNLTFDRQSSIRYSSRRARTLESLASLY 508
KLLHN+ + +++ G E+ KN + A+ ++ D Q R T++ + +
Sbjct: 214 KLLHNNPKLGHLLIVNEGSGEEERKNASQAARKDSKADNQQ--RKVVHPNGTIQ-IHTNT 270
Query: 509 EEEDLKQTLEESARIQRQYEKYIDFVITNNNSDTTFSKILDAL 551
E+E K S+R + + ++D +N + T K+++ +
Sbjct: 271 EDEIAKSDKNNSSRFDIKLKAFLDAKRLSNGNLTALHKMVNKM 313
>SB_34765| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 242
Score = 30.3 bits (65), Expect = 4.3
Identities = 17/58 (29%), Positives = 34/58 (58%), Gaps = 3/58 (5%)
Query: 182 DEHGQLIVARILAGSAAAKQALVSVGDVLLEVDGVH-IESE-EQLKEAVAKPNDRVTL 237
D+ G + + +++ G AAA+ + V D +L V+G + ++ E +Q +A+ +VTL
Sbjct: 186 DDFG-IYITKVIPGGAAAQDGRLQVNDCILSVNGTNTVDIEHQQAVDALKAAGSQVTL 242
>SB_25368| Best HMM Match : PID (HMM E-Value=2.7e-22)
Length = 1197
Score = 30.3 bits (65), Expect = 4.3
Identities = 21/77 (27%), Positives = 36/77 (46%), Gaps = 4/77 (5%)
Query: 191 RILAGSAAAKQALVSVGD--VLLEVDGVHIESEEQLKEAVAKPNDRVTLKVGPNLKEKSS 248
+++A AA+ + D V+L+ G H EE+ +P D T+ P + K+
Sbjct: 316 QVIASEAASTPPINKAKDEQVVLKTAGFHTSFEEEPNYETIQPGDYETIPNFPAKETKAK 375
Query: 249 QLT--NKLTISDRKDPN 263
++ NK + SD D N
Sbjct: 376 KIVDFNKFSESDNGDVN 392
>SB_49600| Best HMM Match : RVT_1 (HMM E-Value=0)
Length = 1273
Score = 29.5 bits (63), Expect = 7.6
Identities = 12/48 (25%), Positives = 25/48 (52%)
Query: 504 LASLYEEEDLKQTLEESARIQRQYEKYIDFVITNNNSDTTFSKILDAL 551
L + ++ DL Q ++E R + +D V++NN +K++D +
Sbjct: 330 LLDITDDHDLNQFVKEPTRRNGNTQNILDLVLSNNKDIIRGAKVIDGI 377
>SB_49131| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1163
Score = 29.5 bits (63), Expect = 7.6
Identities = 14/49 (28%), Positives = 25/49 (51%)
Query: 226 EAVAKPNDRVTLKVGPNLKEKSSQLTNKLTISDRKDPNWWQASHVERPE 274
+ V P R+ + + P LKE+ L ++ I+ +P W +S V P+
Sbjct: 693 QPVQHPPRRIAVALRPKLKERLDDLVSQEVITPVTEPTEWISSLVAAPK 741
>SB_59527| Best HMM Match : DUF382 (HMM E-Value=4.1e-26)
Length = 800
Score = 29.5 bits (63), Expect = 7.6
Identities = 18/65 (27%), Positives = 30/65 (46%)
Query: 153 GNGDMPIETVKVVGLRKVPGQPLGLTVTTDEHGQLIVARILAGSAAAKQALVSVGDVLLE 212
GNG + V V G V G +G V G +++ ++AG A A +V +E
Sbjct: 735 GNGAVAGVVVVVAGGCGVAGVVVGAAVVVVGGGGVVIVVVVAGDAVVVVAEAAVDFYDIE 794
Query: 213 VDGVH 217
++ +H
Sbjct: 795 IEAIH 799
>SB_25064| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1134
Score = 29.5 bits (63), Expect = 7.6
Identities = 13/47 (27%), Positives = 22/47 (46%)
Query: 353 TRGVGRRTLKNRLIQDYPDRFGAVVPHTSRPPRPMEENGQSYWFVSR 399
TR V L NR ++D+ F V RP +N ++++ S+
Sbjct: 102 TRAVHLEVLPNRTLEDFKQAFTGFVARLGRPETIYSDNAKTFFAASK 148
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.317 0.134 0.389
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,374,493
Number of Sequences: 59808
Number of extensions: 766811
Number of successful extensions: 1920
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 1857
Number of HSP's gapped (non-prelim): 71
length of query: 567
length of database: 16,821,457
effective HSP length: 86
effective length of query: 481
effective length of database: 11,677,969
effective search space: 5617103089
effective search space used: 5617103089
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)
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