Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001318-TA|BGIBMGA001318-PA|IPR002048|Calcium-binding
EF-hand
(72 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 97 2e-22
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 58 2e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 54 2e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 48 2e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 47 3e-07
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 32 0.008
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 29 0.057
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 28 0.13
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 28 0.17
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 26 0.70
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 0.92
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 24 2.1
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 24 2.1
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 24 2.8
SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 23 3.7
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 23 6.5
SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharom... 23 6.5
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 22 8.6
SPAC664.03 |||RNA polymerase II associated Paf1 complex |Schizos... 22 8.6
SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomy... 22 8.6
SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomy... 22 8.6
SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4 |S... 22 8.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 97.5 bits (232), Expect = 2e-22
Identities = 46/60 (76%), Positives = 51/60 (85%)
Query: 4 MAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDAD 63
M LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDAD
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Score = 57.2 bits (132), Expect = 2e-10
Identities = 27/63 (42%), Positives = 39/63 (61%)
Query: 1 MVVMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 60
+ +MA + E +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E
Sbjct: 71 LTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA 130
Query: 61 DAD 63
D D
Sbjct: 131 DTD 133
Score = 29.1 bits (62), Expect = 0.075
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Query: 15 AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 63
AE ++ + D DG+GTI E T+M R + E E+++ D D
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKD 97
Score = 25.4 bits (53), Expect = 0.92
Identities = 14/38 (36%), Positives = 24/38 (63%), Gaps = 5/38 (13%)
Query: 7 DQLTEEQIAEF-KEAFSLFDKDGDGTITTKELGTVMRS 43
++L++E++A+ +EA D DGDG I +E V+ S
Sbjct: 116 ERLSQEEVADMIREA----DTDGDGVINYEEFSRVISS 149
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 57.6 bits (133), Expect = 2e-10
Identities = 29/62 (46%), Positives = 43/62 (69%), Gaps = 1/62 (1%)
Query: 10 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADAYEKWR 69
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+ DA ++ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL-GDAIDEKK 62
Query: 70 YI 71
++
Sbjct: 63 FM 64
Score = 41.9 bits (94), Expect = 1e-05
Identities = 19/50 (38%), Positives = 30/50 (60%)
Query: 12 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 61
E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E D
Sbjct: 75 ESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 54.0 bits (124), Expect = 2e-09
Identities = 24/54 (44%), Positives = 35/54 (64%)
Query: 8 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 61
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E + M ++
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
Score = 24.6 bits (51), Expect = 1.6
Identities = 14/53 (26%), Positives = 23/53 (43%), Gaps = 3/53 (5%)
Query: 19 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADA---YEKW 68
EAFS FD G I + + S+G E++ ++ + YEK+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHGVFYYEKF 173
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 48.0 bits (109), Expect = 2e-07
Identities = 20/47 (42%), Positives = 33/47 (70%)
Query: 17 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDAD 63
+K+AFSLFD+ G G I +G ++R+ GQNPT AE+ ++ + + A+
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAE 54
Score = 37.1 bits (82), Expect = 3e-04
Identities = 17/45 (37%), Positives = 26/45 (57%)
Query: 16 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 60
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV 122
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 47.2 bits (107), Expect = 3e-07
Identities = 22/54 (40%), Positives = 32/54 (59%)
Query: 8 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 61
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + D
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFD 83
Score = 45.6 bits (103), Expect = 8e-07
Identities = 23/61 (37%), Positives = 32/61 (52%)
Query: 3 VMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDA 62
VM + + + E K AF LFD D G I+ + L V + L +N + EL+ MI E D
Sbjct: 98 VMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDL 157
Query: 63 D 63
D
Sbjct: 158 D 158
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 32.3 bits (70), Expect = 0.008
Identities = 18/47 (38%), Positives = 26/47 (55%)
Query: 12 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMIN 58
E E +EAF LFD G I ++L LG+N T+ +LQ M++
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLD 55
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 29.5 bits (63), Expect = 0.057
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
Query: 2 VVMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 61
+V + +EQ +E + S+ + T TT+ SLG T+A+L++ +N +
Sbjct: 1581 LVQKVSERLQEQASEKDDKSSVESEHSILTSTTRT-----PSLGIYTTDAQLRNFLNRIS 1635
Query: 62 ADAYEK 67
DAYE+
Sbjct: 1636 IDAYEE 1641
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 28.3 bits (60), Expect = 0.13
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Query: 7 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 60
D+ E A + +S+FD+D +G IT +E+ +G + A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593
>SPAC11G7.02 |pub1||ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 767
Score = 27.9 bits (59), Expect = 0.17
Identities = 13/55 (23%), Positives = 25/55 (45%)
Query: 1 MVVMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQD 55
+ ++AAD L + + F + F++ DG+ T TT + + E + D
Sbjct: 12 VTIVAADGLYKRDVFRFPDPFAVLTVDGEQTHTTTAIKKTLNPYWNETFEVNVTD 66
>SPBC16E9.11c |pub3||ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 786
Score = 25.8 bits (54), Expect = 0.70
Identities = 11/33 (33%), Positives = 20/33 (60%)
Query: 3 VMAADQLTEEQIAEFKEAFSLFDKDGDGTITTK 35
++AAD L++ + + F++ DG+ T TTK
Sbjct: 12 IVAADGLSKRDLFRQPDPFAILTVDGEQTHTTK 44
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 0.92
Identities = 9/24 (37%), Positives = 14/24 (58%)
Query: 19 EAFSLFDKDGDGTITTKELGTVMR 42
+ F FD+D DG + +EL + R
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFR 334
>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 24.2 bits (50), Expect = 2.1
Identities = 9/33 (27%), Positives = 18/33 (54%)
Query: 21 FSLFDKDGDGTITTKELGTVMRSLGQNPTEAEL 53
F KD +TTK++ T+ ++ +PT+ +
Sbjct: 321 FERLRKDSSLQLTTKDINTLFSTIALSPTKLSM 353
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 24.2 bits (50), Expect = 2.1
Identities = 14/57 (24%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Query: 8 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADA 64
+LT E+I + F+ D DG G + + + E+++ I EV+ D+
Sbjct: 12 ELTNEEILTLTDQFNKLDVDGKGYLDQPTTIKAFED-SKKGSYDEVREAIREVNVDS 67
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 23.8 bits (49), Expect = 2.8
Identities = 12/36 (33%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Query: 1 MVVMAADQLTEEQIAEFKEAFSLFDKDGDGTITTKE 36
MV + D+ T E+ + F++ DK+ DG +T +E
Sbjct: 135 MVKLPEDEDTPEK--RVNKIFNMMDKNKDGQLTLEE 168
>SPCC1259.11c |gyp2||GTPase activating protein Gyp2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 720
Score = 23.4 bits (48), Expect = 3.7
Identities = 10/24 (41%), Positives = 15/24 (62%)
Query: 21 FSLFDKDGDGTITTKELGTVMRSL 44
F L+D DGT+ T+E+ + SL
Sbjct: 623 FQLYDLKRDGTLRTEEVVELSESL 646
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 22.6 bits (46), Expect = 6.5
Identities = 10/22 (45%), Positives = 15/22 (68%)
Query: 5 AADQLTEEQIAEFKEAFSLFDK 26
AA + +EQ A++ EA +LF K
Sbjct: 14 AAVAIHKEQTADYVEAIALFQK 35
>SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1690
Score = 22.6 bits (46), Expect = 6.5
Identities = 13/45 (28%), Positives = 21/45 (46%)
Query: 22 SLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADAYE 66
S FD D D +++ E MRS Q+ T ++V ++ E
Sbjct: 1309 SYFDSDSDISMSDNEEDVEMRSEDQSDTSESEVGSKDDVQSEEVE 1353
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 22.2 bits (45), Expect = 8.6
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 9 LTEEQIAEFKEAFSLFD-KDG 28
L +EQ+++F FSL D +DG
Sbjct: 478 LKDEQLSQFDTVFSLTDERDG 498
>SPAC664.03 |||RNA polymerase II associated Paf1 complex
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 457
Score = 22.2 bits (45), Expect = 8.6
Identities = 9/27 (33%), Positives = 14/27 (51%)
Query: 44 LGQNPTEAELQDMINEVDADAYEKWRY 70
L T E+Q +N+ AD +E + Y
Sbjct: 255 LPSEETAEEIQAKVNDASADVHEPFVY 281
>SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 538
Score = 22.2 bits (45), Expect = 8.6
Identities = 12/43 (27%), Positives = 19/43 (44%)
Query: 26 KDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADAYEKW 68
+D D +T KEL + + L +P E + + DA W
Sbjct: 291 RDVDSVLTPKELVEMFKFLRIDPIELTKNPIPFQQSTDAIPFW 333
>SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 764
Score = 22.2 bits (45), Expect = 8.6
Identities = 10/26 (38%), Positives = 15/26 (57%)
Query: 9 LTEEQIAEFKEAFSLFDKDGDGTITT 34
L +E +A +KEA+ K G +TT
Sbjct: 222 LPQEILASYKEAYETLGKIGKLILTT 247
>SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 22.2 bits (45), Expect = 8.6
Identities = 7/11 (63%), Positives = 10/11 (90%)
Query: 58 NEVDADAYEKW 68
+EVD++AYE W
Sbjct: 92 SEVDSEAYEDW 102
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.314 0.130 0.357
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 296,518
Number of Sequences: 5004
Number of extensions: 9012
Number of successful extensions: 39
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 11
Number of HSP's gapped (non-prelim): 30
length of query: 72
length of database: 2,362,478
effective HSP length: 52
effective length of query: 20
effective length of database: 2,102,270
effective search space: 42045400
effective search space used: 42045400
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 45 (22.2 bits)
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