SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001310-TA|BGIBMGA001310-PA|IPR001478|PDZ/DHR/GLGF,
IPR008283|Peptidase M17, leucyl aminopeptidase, N-terminal,
IPR000819|Peptidase M17, leucyl aminopeptidase, C-terminal
         (812 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g30910.1 68417.m04389 cytosol aminopeptidase family protein c...    96   7e-20
At2g24200.1 68415.m02891 cytosol aminopeptidase identical to cyt...    96   7e-20
At4g30920.1 68417.m04390 cytosol aminopeptidase family protein c...    96   9e-20
At1g31600.3 68414.m03879 oxidoreductase, 2OG-Fe(II) oxygenase fa...    48   2e-05
At1g31600.2 68414.m03877 oxidoreductase, 2OG-Fe(II) oxygenase fa...    48   2e-05
At1g31600.1 68414.m03878 oxidoreductase, 2OG-Fe(II) oxygenase fa...    48   2e-05
At4g20350.1 68417.m02970 expressed protein hypothetical protein ...    43   0.001
At4g02485.1 68417.m00337 oxidoreductase, 2OG-Fe(II) oxygenase fa...    39   0.012
At4g14760.1 68417.m02271 M protein repeat-containing protein con...    37   0.047
At3g52370.1 68416.m05756 beta-Ig-H3 domain-containing protein / ...    35   0.19 
At2g35860.1 68415.m04403 beta-Ig-H3 domain-containing protein / ...    35   0.25 
At1g64540.1 68414.m07316 F-box family protein contains F-box dom...    31   3.1  
At5g51530.1 68418.m06390 ubiquitin carboxyl-terminal hydrolase-r...    30   7.1  
At2g20150.1 68415.m02356 expressed protein  and genefinder             30   7.1  
At4g36390.1 68417.m05170 radical SAM domain-containing protein /...    29   9.3  

>At4g30910.1 68417.m04389 cytosol aminopeptidase family protein
           contains Pfam profiles: PF00883 cytosol aminopeptidase
           family catalytic domain, PF02789: cytosol aminopeptidase
           family N-terminal domain
          Length = 581

 Score = 96.3 bits (229), Expect = 7e-20
 Identities = 75/278 (26%), Positives = 125/278 (44%), Gaps = 34/278 (12%)

Query: 371 LSDMPANKMTPIDLGQAA--LDTLCPLGVTVEAREREWIEAQNMQAFLTVARGSCEEPIF 428
           L + PAN ++P  L + A  L ++    +T    + E  +   M ++L VA  S   P F
Sbjct: 270 LVNSPANVLSPAVLAEEASNLASMYSNVMTANILKEEQCKELKMGSYLAVAAASANPPHF 329

Query: 429 LECNYRGDKES-RPPVVIAAKGVTFDSGGLCLKKRESMVENRGSMXXXXXXXXXXXXXXX 487
           +   Y+      +  + +  KG+TFDSGG  +K    ++     +               
Sbjct: 330 IHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKIGPELIIELMKIDVGGSAAVLGAAKAI 389

Query: 488 XQVP---INVCAVIPICENMVSGQCMKVGDVVRALNGLSIQFPPSCKIGRLTNHSYSIKK 544
            ++    + V  ++  CENM+SG  M+ GDV+ A NG +I+   +   GRLT     +  
Sbjct: 390 GEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNDTDSEGRLTLADALV-- 447

Query: 545 RYCRYGCSIEDTDMEGRLMLADALVYGQAVHKPELVIDVATFTRGVLQALGGAACGCYTE 604
               Y C+                   Q V K   ++D+AT T  ++ ALG +  G YT 
Sbjct: 448 ----YACN-------------------QGVDK---IVDIATLTGEIIVALGPSMAGMYTA 481

Query: 605 SEALWRDVTAAGARSGDRAWRLPLWDYYRRQITGGPAE 642
           S+ L ++V AA  RSG++ WR+P+ + Y   +  G A+
Sbjct: 482 SDELAKEVIAASQRSGEKLWRMPMEESYWEMMKSGVAD 519


>At2g24200.1 68415.m02891 cytosol aminopeptidase identical to
           cytosol aminopeptidase SP:P30184 from [Arabidopsis
           thaliana]; contains Pfam profiles: PF00883 cytosol
           aminopeptidase family catalytic domain, PF02789: cytosol
           aminopeptidase family N-terminal domain
          Length = 520

 Score = 96.3 bits (229), Expect = 7e-20
 Identities = 83/288 (28%), Positives = 126/288 (43%), Gaps = 35/288 (12%)

Query: 367 WARFLSDMPANKMTPIDLGQAALDTLCPLG--VTVEAREREWIEAQNMQAFLTVARGSCE 424
           + R L + PAN +TP  L + A           T      E  +   M ++L VA  S  
Sbjct: 204 FGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANILNEEQCKELKMGSYLAVAAASAN 263

Query: 425 EPIFLECNYRGDKES-RPPVVIAAKGVTFDSGGLCLKKRE--SMVENRGSMXXXXXXXXX 481
            P F+   Y+    S +  + +  KG+TFDSGG  +K     S+   +  M         
Sbjct: 264 PPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKTGPGCSIELMKFDMGGSAAVLGA 323

Query: 482 XXXXXXXQVP-INVCAVIPICENMVSGQCMKVGDVVRALNGLSIQFPPSCKIGRLTNHSY 540
                  + P + V  ++  CENM+SG  M+ GDV+ A NG +I+   +   GRLT    
Sbjct: 324 AKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNNTDAEGRLTLADA 383

Query: 541 SIKKRYCRYGCSIEDTDMEGRLMLADALVYGQAVHKPELVIDVATFTRGVLQALGGAACG 600
            +      Y C+                   Q V K   ++D+AT T   + ALG +  G
Sbjct: 384 LV------YACN-------------------QGVDK---IVDLATLTGACVIALGTSMAG 415

Query: 601 CYTESEALWRDVTAAGARSGDRAWRLPLWDYYRRQITGGPAEGLVRAG 648
            YT S+ L ++V AA  RSG++ WR+PL + Y   +  G A+ +V  G
Sbjct: 416 IYTPSDELAKEVIAASERSGEKLWRMPLEESYWEMMKSGVAD-MVNTG 462


>At4g30920.1 68417.m04390 cytosol aminopeptidase family protein
           contains Pfam profiles: PF00883 cytosol aminopeptidase
           family catalytic domain, PF02789: cytosol aminopeptidase
           family N-terminal domain
          Length = 583

 Score = 95.9 bits (228), Expect = 9e-20
 Identities = 82/284 (28%), Positives = 127/284 (44%), Gaps = 35/284 (12%)

Query: 371 LSDMPANKMTPIDLGQAALDTLCPLG--VTVEAREREWIEAQNMQAFLTVARGSCEEPIF 428
           L + PAN +TP  L + AL+        +T      E  +   M ++L VA  S   P F
Sbjct: 271 LVNSPANVLTPAVLAEEALNLASMYSDVMTANILNEEQCKELKMGSYLAVAAASANPPHF 330

Query: 429 LECNYRGDKES-RPPVVIAAKGVTFDSGGLCLKKRES-MVE-NRGSMXXXXXXXXXXXXX 485
           +   Y+      +  + +  KG+TFDSGG  +K     ++E  +  M             
Sbjct: 331 IHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKTGPGCLIELMKFDMGGSAAVLGAAKAI 390

Query: 486 XXXQVP-INVCAVIPICENMVSGQCMKVGDVVRALNGLSIQFPPSCKIGRLTNHSYSIKK 544
              + P + V  ++  CENM+SG  M+ GDV+ A NG +I+   +   GRLT     +  
Sbjct: 391 GQIKPPGVEVHFIVAACENMISGTGMRPGDVLTASNGKTIEVNNTDAEGRLTLADALV-- 448

Query: 545 RYCRYGCSIEDTDMEGRLMLADALVYGQAVHKPELVIDVATFTRGVLQALGGAACGCYTE 604
               Y C+                   Q V K   V+D+AT T   + ALG +  G YT 
Sbjct: 449 ----YACN-------------------QGVDK---VVDLATLTGACIIALGTSMAGIYTP 482

Query: 605 SEALWRDVTAAGARSGDRAWRLPLWDYYRRQITGGPAEGLVRAG 648
           S+ L ++V AA  RSG++ WR+P+ + Y   +  G A+ +V  G
Sbjct: 483 SDKLAKEVIAASERSGEKLWRMPMEESYWEMMKSGVAD-MVNTG 525


>At1g31600.3 68414.m03879 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein contains Pfam profiles PF03171:
           oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
           RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
          Length = 431

 Score = 48.0 bits (109), Expect = 2e-05
 Identities = 51/180 (28%), Positives = 88/180 (48%), Gaps = 31/180 (17%)

Query: 17  AEEQLILSNIYAAPKPKWTQLSNRRLQNWG-----GVPH--NKGMIAETIPTWLDLYLER 69
           AEEQ +L+ + A     W  L+ RR+Q++G     G  +   K  + E +P+++   LER
Sbjct: 223 AEEQQLLAAVDAR---HWIGLAKRRVQHYGYEFCYGTRNVDTKKRLGE-LPSFVSPILER 278

Query: 70  IHKL----NVMCGRRPNHVLVNEYTPGQGIMPHLD-GNLFYPTITTISVGSHIVLKFLK- 123
           I+      N       + + VNEY  G G+ PH+D  + F   I ++S+    +++F + 
Sbjct: 279 IYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRY 338

Query: 124 -------PTTDNEKVEMQPMF--SFLLEPRSLLILQDK---LFSHYL--HSIEELNEDVI 169
                   TTD EK         +  L PRS+L+L  +    ++HY+  H I+++ + VI
Sbjct: 339 SVSTWKASTTDAEKSGDSSCIKKALYLPPRSMLLLSGEARYAWNHYIPHHKIDKVKDKVI 398


>At1g31600.2 68414.m03877 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein contains Pfam profiles PF03171:
           oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
           RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
          Length = 344

 Score = 48.0 bits (109), Expect = 2e-05
 Identities = 51/180 (28%), Positives = 88/180 (48%), Gaps = 31/180 (17%)

Query: 17  AEEQLILSNIYAAPKPKWTQLSNRRLQNWG-----GVPH--NKGMIAETIPTWLDLYLER 69
           AEEQ +L+ + A     W  L+ RR+Q++G     G  +   K  + E +P+++   LER
Sbjct: 136 AEEQQLLAAVDAR---HWIGLAKRRVQHYGYEFCYGTRNVDTKKRLGE-LPSFVSPILER 191

Query: 70  IHKL----NVMCGRRPNHVLVNEYTPGQGIMPHLD-GNLFYPTITTISVGSHIVLKFLK- 123
           I+      N       + + VNEY  G G+ PH+D  + F   I ++S+    +++F + 
Sbjct: 192 IYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRY 251

Query: 124 -------PTTDNEKVEMQPMF--SFLLEPRSLLILQDK---LFSHYL--HSIEELNEDVI 169
                   TTD EK         +  L PRS+L+L  +    ++HY+  H I+++ + VI
Sbjct: 252 SVSTWKASTTDAEKSGDSSCIKKALYLPPRSMLLLSGEARYAWNHYIPHHKIDKVKDKVI 311


>At1g31600.1 68414.m03878 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein contains Pfam profiles PF03171:
           oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
           RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
          Length = 431

 Score = 48.0 bits (109), Expect = 2e-05
 Identities = 51/180 (28%), Positives = 88/180 (48%), Gaps = 31/180 (17%)

Query: 17  AEEQLILSNIYAAPKPKWTQLSNRRLQNWG-----GVPH--NKGMIAETIPTWLDLYLER 69
           AEEQ +L+ + A     W  L+ RR+Q++G     G  +   K  + E +P+++   LER
Sbjct: 223 AEEQQLLAAVDAR---HWIGLAKRRVQHYGYEFCYGTRNVDTKKRLGE-LPSFVSPILER 278

Query: 70  IHKL----NVMCGRRPNHVLVNEYTPGQGIMPHLD-GNLFYPTITTISVGSHIVLKFLK- 123
           I+      N       + + VNEY  G G+ PH+D  + F   I ++S+    +++F + 
Sbjct: 279 IYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRY 338

Query: 124 -------PTTDNEKVEMQPMF--SFLLEPRSLLILQDK---LFSHYL--HSIEELNEDVI 169
                   TTD EK         +  L PRS+L+L  +    ++HY+  H I+++ + VI
Sbjct: 339 SVSTWKASTTDAEKSGDSSCIKKALYLPPRSMLLLSGEARYAWNHYIPHHKIDKVKDKVI 398


>At4g20350.1 68417.m02970 expressed protein hypothetical protein -
           Caenorhabditis elegans,SPTREMBL:Q17527
          Length = 139

 Score = 42.7 bits (96), Expect = 0.001
 Identities = 27/83 (32%), Positives = 41/83 (49%), Gaps = 15/83 (18%)

Query: 96  MPHLDGNLFYPTITTISVGSHIVLKF-----LKPTTDNEKVEMQP----------MFSFL 140
           MPH DG  ++P +  +S+GS +V+ F     L+        +  P           FS L
Sbjct: 1   MPHQDGPAYFPVVAILSLGSPVVMDFTPHLRLRSGDGYISKDQSPCAESCAPERDSFSVL 60

Query: 141 LEPRSLLILQDKLFSHYLHSIEE 163
           L P+SLLI +D  +S +LH I +
Sbjct: 61  LMPQSLLIFKDDAYSDFLHGISD 83


>At4g02485.1 68417.m00337 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein contains Pfam domain PF03171, 2OG-Fe(II)
           oxygenase superfamily
          Length = 226

 Score = 39.1 bits (87), Expect = 0.012
 Identities = 25/80 (31%), Positives = 39/80 (48%), Gaps = 5/80 (6%)

Query: 82  NHVLVNEYTPGQGIMPHLDGNLFYPTITTISVGSHIVLKFLKPTTDNEKVEMQPMFSFLL 141
           + ++VN Y PG+GI  H+D   F   I  +S+ S  V++F  P   NE          LL
Sbjct: 121 DQLIVNLYQPGEGICAHVDLLRFEDGIAIVSLESPCVMRF-SPAEKNE----YEAVDVLL 175

Query: 142 EPRSLLILQDKLFSHYLHSI 161
            P SL+++  +    + H I
Sbjct: 176 NPGSLILMSGEARYRWKHEI 195


>At4g14760.1 68417.m02271 M protein repeat-containing protein contains
            Pfam profile: PF02370 M protein repeat
          Length = 1676

 Score = 37.1 bits (82), Expect = 0.047
 Identities = 25/108 (23%), Positives = 54/108 (50%), Gaps = 4/108 (3%)

Query: 133  MQPMFSFLLEPRSLLILQDKLFSHYLHSIEELNEDVIDDSIVNLNMC--SDKYVREASGE 190
            +Q  +S+ L     L+L+   F   +H +EE N+ ++ +++   N C     +  E + E
Sbjct: 994  LQQDYSYTLNDNKTLLLKFSEFKDGMHVVEEENDAILQEAVALSNTCVVYRSFGSEMAEE 1053

Query: 191  INQKLD--SSKKGLVLGVYQCGKKLELTPVGQELDQKSGGKILQHLNE 236
            +   ++  SS + +  G+ +  + LE    G+E + +   K+L++L E
Sbjct: 1054 VEDFVETVSSLREISTGLKRKVETLEKKLEGKEKESQGLNKMLENLQE 1101


>At3g52370.1 68416.m05756 beta-Ig-H3 domain-containing protein /
           fasciclin domain-containing protein weak similarity to
           osteoblast specific factor 2 [Homo sapiens] GI:393319;
           contains Pfam profile PF02469: Fasciclin domain;
           supporting cDNA gi|26450295|dbj|AK117608.1|
          Length = 436

 Score = 35.1 bits (77), Expect = 0.19
 Identities = 23/82 (28%), Positives = 41/82 (50%), Gaps = 4/82 (4%)

Query: 112 SVGSHIVLKFLKPTTDNEKVEMQPMF-SFLLEPRSLLILQDKLFSHYLHSIEELNEDVID 170
           +VG H +  F  P  D  +  + P F SFLL+P++L  LQ  L  H L   + +      
Sbjct: 63  AVGQHNITIFA-PRNDALEKNLDPEFKSFLLQPKNLKSLQSLLMFHILP--KRITSPQFS 119

Query: 171 DSIVNLNMCSDKYVREASGEIN 192
            ++V+    S+ ++   +G++N
Sbjct: 120 SAVVSHRTLSNDHLHFTNGKVN 141


>At2g35860.1 68415.m04403 beta-Ig-H3 domain-containing protein /
           fasciclin domain-containing protein contains Pfam
           profile PF02469: Fasciclin domain
          Length = 445

 Score = 34.7 bits (76), Expect = 0.25
 Identities = 21/48 (43%), Positives = 27/48 (56%), Gaps = 2/48 (4%)

Query: 112 SVGSHIVLKFLKPTTDNEKVEMQPMF-SFLLEPRSLLILQDKLFSHYL 158
           +VG H +  F  P  D  +  + P+F SFLLEPR+L  LQ  L  H L
Sbjct: 67  AVGKHNITIFA-PRNDALERNLDPLFKSFLLEPRNLKSLQSLLMFHIL 113


>At1g64540.1 68414.m07316 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 444

 Score = 31.1 bits (67), Expect = 3.1
 Identities = 11/23 (47%), Positives = 19/23 (82%), Gaps = 1/23 (4%)

Query: 93  QGIMPHLDGNLFYPTITTISVGS 115
           QG++PH DG +F+P + T+S+G+
Sbjct: 132 QGLVPH-DGTVFFPALKTLSLGA 153


>At5g51530.1 68418.m06390 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1149

 Score = 29.9 bits (64), Expect = 7.1
 Identities = 16/37 (43%), Positives = 20/37 (54%)

Query: 118 VLKFLKPTTDNEKVEMQPMFSFLLEPRSLLILQDKLF 154
           V  FL  T   EK++  P FSFLL  + LL  +D  F
Sbjct: 429 VNSFLDHTRVKEKIDFDPQFSFLLLDKRLLKCKDVPF 465


>At2g20150.1 68415.m02356 expressed protein  and genefinder
          Length = 93

 Score = 29.9 bits (64), Expect = 7.1
 Identities = 18/39 (46%), Positives = 25/39 (64%), Gaps = 3/39 (7%)

Query: 128 NEKVEMQPMFSFLLE--PRSLLILQDKLFSHYLHSIEEL 164
           +E + M+ + SF LE  P S+  L+DKLFS YL  +E L
Sbjct: 24  SESITMERIVSFTLEEVPESIAKLEDKLFS-YLLDLEGL 61


>At4g36390.1 68417.m05170 radical SAM domain-containing protein /
           TRAM domain-containing protein similar to CDK5
           activator-binding protein [Rattus norvegicus]
           GI:7330738; contains Pfam profiles PF00919: UPF0004
           family protein, PF01938: TRAM domain, PF04055: radical
           SAM domain protein
          Length = 640

 Score = 29.5 bits (63), Expect = 9.3
 Identities = 25/85 (29%), Positives = 39/85 (45%), Gaps = 3/85 (3%)

Query: 373 DMPANKMTPIDLGQAALDTLCPLGVTVEAREREWIEAQNMQAFLTVARGSCEEPIFLECN 432
           D+P   +  +D GQ  ++TL  L  T        I   ++ AF++V RG      F    
Sbjct: 248 DLP-RLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVMRGCNNMCAFCIVP 306

Query: 433 Y-RGDKESRP-PVVIAAKGVTFDSG 455
           + RG + SRP   +I   G  ++SG
Sbjct: 307 FTRGRERSRPVESIIREVGELWESG 331


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,507,585
Number of Sequences: 28952
Number of extensions: 776097
Number of successful extensions: 1688
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1668
Number of HSP's gapped (non-prelim): 21
length of query: 812
length of database: 12,070,560
effective HSP length: 87
effective length of query: 725
effective length of database: 9,551,736
effective search space: 6925008600
effective search space used: 6925008600
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)

- SilkBase 1999-2023 -