BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001277-TA|BGIBMGA001277-PA|IPR001128|Cytochrome P450, IPR002401|E-class P450, group I (452 letters) Database: tribolium 317 sequences; 114,650 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value AF251548-1|AAF70178.1| 491|Tribolium castaneum cytochrome P450 ... 90 3e-20 AY337337-2|AAP94193.1| 491|Tribolium castaneum cytochrome P450 ... 89 8e-20 AF254755-1|AAF70496.1| 505|Tribolium castaneum cytochrome P450 ... 77 3e-16 AY337337-1|AAP94192.1| 505|Tribolium castaneum cytochrome P450 ... 74 2e-15 AF265298-1|AAG17641.1| 124|Tribolium castaneum putative cytochr... 52 6e-09 AF263514-1|AAF74206.1| 127|Tribolium castaneum cytochrome P450 ... 50 3e-08 AY920544-1|AAX62142.1| 463|Tribolium castaneum cytochrome P450 ... 49 6e-08 AF264718-1|AAF75271.1| 125|Tribolium castaneum putative cytochr... 46 4e-07 AF264721-1|AAF75273.1| 126|Tribolium castaneum putative cytochr... 42 9e-06 AF264720-1|AAF75272.1| 126|Tribolium castaneum putative cytochr... 41 2e-05 AF265297-1|AAG17640.1| 125|Tribolium castaneum putative cytochr... 40 5e-05 AF265213-1|AAF75277.1| 143|Tribolium castaneum putative cytochr... 39 6e-05 AF263515-1|AAF74207.1| 126|Tribolium castaneum cytochrome P450 ... 39 8e-05 AF265300-1|AAG17643.1| 126|Tribolium castaneum putative cytochr... 36 8e-04 AF265299-1|AAG17642.1| 63|Tribolium castaneum putative cytochr... 35 0.001 AM292374-1|CAL23186.2| 659|Tribolium castaneum gustatory recept... 23 5.8 AM292345-1|CAL23157.2| 384|Tribolium castaneum gustatory recept... 23 5.8 >AF251548-1|AAF70178.1| 491|Tribolium castaneum cytochrome P450 monooxigenase CYP4Q4 protein. Length = 491 Score = 89.8 bits (213), Expect = 3e-20 Identities = 87/296 (29%), Positives = 135/296 (45%), Gaps = 33/296 (11%) Query: 149 WALESIGVVAFGIRLNCFDPNLAENSPEKKLIECVHQIFNLSNQLDFQPSLWH--IFS-T 205 + L SIG A GI+LN A ++ + V++I L +P +++ I+S T Sbjct: 175 FTLLSIGETAMGIKLN------ASDNDKDGYKRAVYKIGQLLTYRAPRPWIYNETIYSLT 228 Query: 206 PTYKKAMKMFQLQEDLSKYFINKAMRNINKNENKPDEQKGVLEKLLDINEEYAYI----- 260 P +K K+ + S I + ++ + + ++ +L+ LL E A I Sbjct: 229 PQGRKEQKVLKSLHSFSNNVIAERKKHFSSSSYSSRKRLAMLDLLLKYKSEGANIDDEGI 288 Query: 261 --MATDMLVAGVDTVANSIAATLYLFAKNPEKQEKLREE---VMSKESKRYLKA------ 309 + G DT + SI TL L A + E QE++ +E V+ +E Y K Sbjct: 289 REEVDTFMFEGHDTTSVSICYTLMLLANHREVQEEILKEMEAVLDEEPPTYAKLQELKFM 348 Query: 310 --CIKETMRMMPVVSGNLRRTTKEYNI-LGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFI 366 IKE++R+ P V R + E GY +PK V D+ +P P +F Sbjct: 349 DRVIKESLRLYPSVPFISRVSGSEIQTKTGYTIPKDCMVNLQIYDMHHNPNVFPDPEKFD 408 Query: 367 PERWLADKNDPLYYGKAHPFVMAPFGFGVRSCIGRRIAELEIETFLTKILENFRVE 422 P+R+L P K HPF PF G R+CIG++ A LEI+T L IL+ F +E Sbjct: 409 PDRFL-----PENIQKRHPFAYIPFSAGSRNCIGQKFAMLEIKTVLCGILKKFILE 459 >AY337337-2|AAP94193.1| 491|Tribolium castaneum cytochrome P450 monooxygenase protein. Length = 491 Score = 88.6 bits (210), Expect = 8e-20 Identities = 83/293 (28%), Positives = 128/293 (43%), Gaps = 27/293 (9%) Query: 149 WALESIGVVAFGIRLNCFDPNLAENSPEKKLIECVHQIFNLSNQLDFQPSLWHIFSTPTY 208 + L SIG A GI+LN D + K+ + + Q+ + + TP Sbjct: 175 FTLLSIGETAMGIKLNASDN---DKDGYKRAVYKIGQLLTYRAPRPWIHNETIYSLTPQG 231 Query: 209 KKAMKMFQLQEDLSKYFINKAMRNINKNENKPDEQKGVLEKLLDINEEYAYI-------M 261 +K K+ + S I + ++ + + ++ +L+ LL E A I Sbjct: 232 RKEQKVLKSLHSFSNNVIAERKKHFSSSSYSSRKRLAMLDLLLKYKSEGANIDDEGIREE 291 Query: 262 ATDMLVAGVDTVANSIAATLYLFAKNPEKQEKLREE---VMSKESKRYLKA--------C 310 + G DT + SI TL L A + E QE++ +E V+ +E Y K Sbjct: 292 VDTFMFEGHDTTSVSICYTLMLLANHREVQEEILKEMEAVLDEEPPTYAKLQELKFMDRV 351 Query: 311 IKETMRMMPVVSGNLRRTTKEYNI-LGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPER 369 IKE++R+ P V R + E GY +PK V D+ +P P +F P+R Sbjct: 352 IKESLRLYPSVPFISRVSGSEIQTKTGYTIPKDCMVNLQIYDMHHNPNVFPDPEKFDPDR 411 Query: 370 WLADKNDPLYYGKAHPFVMAPFGFGVRSCIGRRIAELEIETFLTKILENFRVE 422 +L P K HPF PF G R+CIG++ A LEI+T L IL+ F +E Sbjct: 412 FL-----PENIQKRHPFAYIPFSAGSRNCIGQKFAMLEIKTVLCGILKKFILE 459 >AF254755-1|AAF70496.1| 505|Tribolium castaneum cytochrome P450 monooxigenase CYP4Q7 protein. Length = 505 Score = 76.6 bits (180), Expect = 3e-16 Identities = 54/177 (30%), Positives = 84/177 (47%), Gaps = 19/177 (10%) Query: 269 GVDTVANSIAATLYLFAKNPEKQEKLREEV-------------MSKESKRYLKACIKETM 315 G DT A ++ L + A + E Q+K+ EE+ + +YL+ C+KE + Sbjct: 313 GHDTTAAALGFALMVLAGHKEVQDKIVEEMNEVLGDIKKKPTYQDLQEMKYLERCVKEVL 372 Query: 316 RMMPVVSGNLRRTTKEY-NILGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPERWLADK 374 R+ P V R+ ++ G+ + KG V DL YP P +F P+R+L + Sbjct: 373 RLYPSVHFISRKLGEDLVTHSGHKLAKGSIVNLHIYDLHHNPAIYPDPEKFDPDRFLPEN 432 Query: 375 NDPLYYGKAHPFVMAPFGFGVRSCIGRRIAELEIETFLTKILENFRVEWYGPPPKII 431 K HPF PF G R+CIG++ A LE++ + IL NF +E P I+ Sbjct: 433 CQ-----KRHPFAYLPFSAGPRNCIGKKFAMLELKAAICGILANFTLEPIDIPETIV 484 >AY337337-1|AAP94192.1| 505|Tribolium castaneum cytochrome P450 monooxygenase protein. Length = 505 Score = 74.1 bits (174), Expect = 2e-15 Identities = 53/177 (29%), Positives = 83/177 (46%), Gaps = 19/177 (10%) Query: 269 GVDTVANSIAATLYLFAKNPEKQEKLREEV-------------MSKESKRYLKACIKETM 315 G DT A ++ L + A + E Q+K+ EE+ + +YL+ C+KE + Sbjct: 313 GHDTTAAALGFALMVLAGHKEVQDKIVEEMNEVLGDIKKKPTYQDLQEMKYLERCVKEVL 372 Query: 316 RMMPVVSGNLRRTTKEY-NILGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPERWLADK 374 R+ P V R+ ++ G+ + K V DL YP P +F P+R+L + Sbjct: 373 RLYPSVHFISRKLGEDLVTHSGHKLAKASIVNLHIYDLHHNPAIYPDPEKFDPDRFLPEN 432 Query: 375 NDPLYYGKAHPFVMAPFGFGVRSCIGRRIAELEIETFLTKILENFRVEWYGPPPKII 431 K HPF PF G R+CIG++ A LE++ + IL NF +E P I+ Sbjct: 433 CQ-----KRHPFAYLPFSAGPRNCIGKKFAMLELKAAICGILANFTLEPIDIPETIV 484 >AF265298-1|AAG17641.1| 124|Tribolium castaneum putative cytochrome P450 monooxigenase protein. Length = 124 Score = 52.4 bits (120), Expect = 6e-09 Identities = 34/111 (30%), Positives = 54/111 (48%), Gaps = 12/111 (10%) Query: 275 NSIAATLYLFAKNPEKQEKLREEVMSK------------ESKRYLKACIKETMRMMPVVS 322 + IA + A+NP+ QEKL EEV + + +YL+ +KE R+ P V Sbjct: 3 SGIAFAILCLAENPKVQEKLYEEVAAVIDNIENITMQQLQEMKYLEMVLKEAQRLYPSVP 62 Query: 323 GNLRRTTKEYNILGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPERWLAD 373 RR + NI GY PK ++ + E+++P P +F P R+L + Sbjct: 63 VIERRLEVDCNIGGYDFPKDTFLSLFIYGMHHNEKYFPEPEKFDPNRYLPE 113 >AF263514-1|AAF74206.1| 127|Tribolium castaneum cytochrome P450 monooxygenase protein. Length = 127 Score = 50.0 bits (114), Expect = 3e-08 Identities = 24/78 (30%), Positives = 41/78 (52%), Gaps = 2/78 (2%) Query: 305 RYLKACIKETMRMMPVVSGNLRRTTKEYNILG--YHVPKGIDVAFAHQDLSSMEEHYPRP 362 +YL+ C+ ET+RM P V R+ ++ + Y VP G V + +EE+YP P Sbjct: 46 KYLERCLMETLRMFPPVPIIARQLNQDLKLASGDYTVPAGCTVVIGTFKVHRLEEYYPNP 105 Query: 363 TEFIPERWLADKNDPLYY 380 +F P+ +L ++ +Y Sbjct: 106 DKFDPDNFLPERTANRHY 123 >AY920544-1|AAX62142.1| 463|Tribolium castaneum cytochrome P450 monooxygenase protein. Length = 463 Score = 49.2 bits (112), Expect = 6e-08 Identities = 45/181 (24%), Positives = 78/181 (43%), Gaps = 25/181 (13%) Query: 241 DEQKGVLEKLLDINEEYAYIMATDMLVAGVDTVANSIAATLYLFAKNPEKQEKLREEVMS 300 + + G K +++ +E A AG +TV+ ++ Y A NP Q+KL++E+ Sbjct: 252 ESEIGKNTKQVELTDEKIVAQALLFFFAGFETVSTGVSFMAYELATNPHVQKKLQKEIDL 311 Query: 301 K-------------ESKRYLKACIKETMRMMPVVSGNLRRTTKEYNILG-------YHVP 340 +S +YL + E++R+ P R K + I + V Sbjct: 312 TLQENHGKISYNVLQSMKYLDQVVCESLRLWPPAPQTDRLCNKNFVIEASKPHERTFTVE 371 Query: 341 KGIDVAFAHQDLSSMEEHYPRPTEFIPERWLADKNDPLYYGKAHPFVMAPFGFGVRSCIG 400 K V + + +++P P +F PER+ +D+N K P PFG G R+CI Sbjct: 372 KDTMVMISMFAIHRDPQYFPDPEKFDPERF-SDENK----AKIVPGTYMPFGVGPRNCIA 426 Query: 401 R 401 + Sbjct: 427 K 427 >AF264718-1|AAF75271.1| 125|Tribolium castaneum putative cytochrome P450 monooxigenaseCYP4Q6 protein. Length = 125 Score = 46.4 bits (105), Expect = 4e-07 Identities = 38/124 (30%), Positives = 58/124 (46%), Gaps = 18/124 (14%) Query: 276 SIAATLYLFAKNPEKQEKLREEVMSKESK------------RYLKACIKETMRMMPVVSG 323 +I+ L A + Q K+REE++S K +Y K CIKET+R+ P V Sbjct: 4 AISFILLTLANLQDIQTKVREEILSVVGKEKIPTYNDLQELKYTKRCIKETLRLFPSVPF 63 Query: 324 NLRRTTKEY-NILGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPERWLADKNDPLYYGK 382 R ++++ G +P+G + DL E YP +F P+R+L DK + Sbjct: 64 ISRYASEDFVTKTGNTIPEGTVLHIHIFDLHRNAEIYPDLLKFDPDRFLPDK-----VNE 118 Query: 383 AHPF 386 HPF Sbjct: 119 RHPF 122 >AF264721-1|AAF75273.1| 126|Tribolium castaneum putative cytochrome P450 monooxigenaseCYP4Q2 protein. Length = 126 Score = 41.9 bits (94), Expect = 9e-06 Identities = 27/86 (31%), Positives = 42/86 (48%), Gaps = 6/86 (6%) Query: 302 ESKRYLKACIKETMRMMPVVSGNLRRTTKEYNIL-GYHVPKGIDVAFAHQDLSSMEEHYP 360 ++ +YL+ CIKE++R+ P V R ++ GY +PK DL + YP Sbjct: 43 QNLKYLERCIKESLRLYPSVHLISRALGEDVRTQKGYLIPKDTITIIHIYDLHHNPDIYP 102 Query: 361 RPTEFIPERWLADKNDPLYYGKAHPF 386 P +F P+R+L P K HP+ Sbjct: 103 DPEKFDPDRFL-----PENVQKRHPY 123 >AF264720-1|AAF75272.1| 126|Tribolium castaneum putative cytochrome P450 monooxigenaseCYP4Q1 protein. Length = 126 Score = 41.1 bits (92), Expect = 2e-05 Identities = 36/124 (29%), Positives = 57/124 (45%), Gaps = 12/124 (9%) Query: 265 MLVAGVDTVANSIAATLYLFAKNPEKQEKLREEVMSKESKRYLKACIKETMRMMPVVSGN 324 ML+A V SI A + + K+ + ++ +YL+ CIKET+R+ P V Sbjct: 10 MLIARHSEVQESIVAEMREVLGDLSKKPSYND----LQNLKYLERCIKETLRLYPSVH-F 64 Query: 325 LRRTTKEYNIL--GYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPERWLADKNDPLYYGK 382 + RT + I GY +PK + D+ + YP P + P+R+L P K Sbjct: 65 ISRTLGQDLITTGGYTLPKESNAIIHIYDVHRNADIYPDPEKLDPDRFL-----PENVQK 119 Query: 383 AHPF 386 HP+ Sbjct: 120 RHPY 123 >AF265297-1|AAG17640.1| 125|Tribolium castaneum putative cytochrome P450 monooxigenase protein. Length = 125 Score = 39.5 bits (88), Expect = 5e-05 Identities = 31/113 (27%), Positives = 53/113 (46%), Gaps = 13/113 (11%) Query: 274 ANSIAATLYLFAKNPEKQEKLREEV---MSKESK---------RYLKACIKETMRMMPVV 321 A +I L A +P+ QE++ EE+ S ++K +Y++ CIKE +R+ P V Sbjct: 2 AVAICFALMCIACHPDIQERIFEEIEETFSDDTKPDYKSLQELKYMERCIKEVLRLYPSV 61 Query: 322 SGNLRRTTKEYNILGYHVPKGIDVAFAH-QDLSSMEEHYPRPTEFIPERWLAD 373 R ++ H K + H D+ + YP P +F P+R+L + Sbjct: 62 PFIARSLGEDIVTYSGHKLKAGSMVHLHIYDMHHNPQVYPDPEKFDPDRFLPE 114 >AF265213-1|AAF75277.1| 143|Tribolium castaneum putative cytochrome P450 monooxigenaseCYP4Q3 protein. Length = 143 Score = 39.1 bits (87), Expect = 6e-05 Identities = 25/86 (29%), Positives = 41/86 (47%), Gaps = 6/86 (6%) Query: 302 ESKRYLKACIKETMRMMPVVSGNLRRTTKEY-NILGYHVPKGIDVAFAHQDLSSMEEHYP 360 + +YL+ IKE++R+ P V R+ +++ G +PK DL + YP Sbjct: 60 QEMKYLERAIKESLRLYPSVHFISRKLGEDFVTCNGLKLPKSTITHLHIYDLHHNPDIYP 119 Query: 361 RPTEFIPERWLADKNDPLYYGKAHPF 386 P +F PER+ + + K HPF Sbjct: 120 DPEKFDPERFRPENSQ-----KRHPF 140 >AF263515-1|AAF74207.1| 126|Tribolium castaneum cytochrome P450 monooxygenase protein. Length = 126 Score = 38.7 bits (86), Expect = 8e-05 Identities = 35/128 (27%), Positives = 58/128 (45%), Gaps = 19/128 (14%) Query: 273 VANSIAATLYLFAKNPEKQEKLREE---VMSKESKR----------YLKACIKETMRMMP 319 V+++I L + A +P QE++ +E V+ K+ Y++ IKE +R+ P Sbjct: 1 VSSAINFALMVLANHPTVQEEIVQEMKDVLGDTKKKPVYNDLQELKYMERVIKEVLRLYP 60 Query: 320 VVSGNLRRTTKEY-NILGYHVPKGIDVAFAHQDLSSMEEHYPRPTEFIPERWLADKNDPL 378 V R ++ GY + KG + DL YP P +F P+R+L + D Sbjct: 61 SVHYISRELGEDMVTTTGYKLRKGTILQLHIYDLHYNPVIYPEPEKFDPDRFLPENCD-- 118 Query: 379 YYGKAHPF 386 K HP+ Sbjct: 119 ---KRHPY 123 >AF265300-1|AAG17643.1| 126|Tribolium castaneum putative cytochrome P450 monooxigenase protein. Length = 126 Score = 35.5 bits (78), Expect = 8e-04 Identities = 20/78 (25%), Positives = 37/78 (47%), Gaps = 2/78 (2%) Query: 305 RYLKACIKETMRMMPVVSGNLRRTTKEYNILG--YHVPKGIDVAFAHQDLSSMEEHYPRP 362 +YL+ + ET+RM P V R +E + Y +P G V + +++P P Sbjct: 46 KYLERVLLETLRMYPPVPIITRVINEEVKLASGDYTLPVGTTVGIGQFLVHRNPKYFPNP 105 Query: 363 TEFIPERWLADKNDPLYY 380 +F P+ +L ++ +Y Sbjct: 106 DKFDPDNFLPERCQQRHY 123 >AF265299-1|AAG17642.1| 63|Tribolium castaneum putative cytochrome P450 monooxigenase protein. Length = 63 Score = 35.1 bits (77), Expect = 0.001 Identities = 24/62 (38%), Positives = 32/62 (51%), Gaps = 14/62 (22%) Query: 274 ANSIAATLYLFAKNPEKQEKLREE---VMSKESK-----------RYLKACIKETMRMMP 319 A+ I+ LY A NPE QEK EE + KE K +YL+ IKE +R+ P Sbjct: 2 ASGISFALYCLANNPEAQEKAYEEQVALFGKEKKPIVSYSDLQEMKYLELVIKEALRLYP 61 Query: 320 VV 321 +V Sbjct: 62 LV 63 >AM292374-1|CAL23186.2| 659|Tribolium castaneum gustatory receptor candidate 53 protein. Length = 659 Score = 22.6 bits (46), Expect = 5.8 Identities = 12/44 (27%), Positives = 23/44 (52%) Query: 212 MKMFQLQEDLSKYFINKAMRNINKNENKPDEQKGVLEKLLDINE 255 +KM +++ + IN + KN+ P E KG ++L +N+ Sbjct: 461 VKMLKIRFVILNKQINNLIEYFQKNKIGPVETKGTNKQLNTLNK 504 >AM292345-1|CAL23157.2| 384|Tribolium castaneum gustatory receptor candidate 24 protein. Length = 384 Score = 22.6 bits (46), Expect = 5.8 Identities = 12/44 (27%), Positives = 23/44 (52%) Query: 212 MKMFQLQEDLSKYFINKAMRNINKNENKPDEQKGVLEKLLDINE 255 +KM +++ + IN + KN+ P E KG ++L +N+ Sbjct: 186 VKMLKIRFVILNKQINNLIEYFQKNKIGPVETKGTNKQLNTLNK 229 Database: tribolium Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 114,650 Number of sequences in database: 317 Lambda K H 0.320 0.136 0.403 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 108,182 Number of Sequences: 317 Number of extensions: 4780 Number of successful extensions: 43 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 2 Number of HSP's gapped (non-prelim): 18 length of query: 452 length of database: 114,650 effective HSP length: 59 effective length of query: 393 effective length of database: 95,947 effective search space: 37707171 effective search space used: 37707171 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 45 (22.2 bits)
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