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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001275-TA|BGIBMGA001275-PA|IPR002052|N-6
Adenine-specific DNA methylase, IPR007484|Peptidase M28
         (409 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g54720.1 68416.m06054 glutamate carboxypeptidase, putative  (...    46   3e-05
At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept...    42   6e-04
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain...    41   0.002
At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransfera...    33   0.26 
At1g30680.1 68414.m03751 toprim domain-containing protein contai...    29   4.2  
At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase, puta...    28   9.8  

>At3g54720.1 68416.m06054 glutamate carboxypeptidase, putative
           (AMP1) identical to GI:15624092 glutamate
           carboxypeptidase {Arabidopsis thaliana}; ileal
           peptidase, Rattus norvegicus, EMBL:AF009921; identical
           to cDNA glutamate carboxypeptidase (AMP1) GI:15624091;
           contains Pfam profiles PF04389: Peptidase family M28,
           PF04253: Transferrin receptor-like dimerisation domain
           and PF02225 PA domain
          Length = 705

 Score = 46.4 bits (105), Expect = 3e-05
 Identities = 26/102 (25%), Positives = 48/102 (47%), Gaps = 3/102 (2%)

Query: 190 KIVLKIHMSYAMSRKISRNTIIDLKGKESPEKQVIVSGHIDSWXXXXXXXXXXXXXXISW 249
           ++V+ +     M  K   N ++ ++G E  ++ VI+  H D+W              +  
Sbjct: 318 RMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAVDPNSGTSALLDI 377

Query: 250 FVPVILNILK--LKPRRTIRAILWTAEEPGLIGAEAYLTKHI 289
                L +LK   +PRRTI    W AEE G+IG+  ++ +++
Sbjct: 378 SRRFAL-LLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENV 418


>At5g19740.1 68418.m02347 peptidase M28 family protein ileal
           peptidase I100 - Rattus norvegicus, EMBL:AF009921;
           contains Pfam profiles PF04389: Peptidase family M28,
           PF02225: PA domain
          Length = 681

 Score = 42.3 bits (95), Expect = 6e-04
 Identities = 29/99 (29%), Positives = 50/99 (50%), Gaps = 7/99 (7%)

Query: 195 IHMSYAMSRKISR--NTIIDLKGKESPEKQVIVSGHIDSWXXXXXXXXXXXXXXISWFVP 252
           +++SY     I++  N I  ++G+E P++ VI+  H D+W              +   + 
Sbjct: 298 LNLSYIGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAVLME--IA 355

Query: 253 VILNILKL---KPRRTIRAILWTAEEPGLIGAEAYLTKH 288
             L+ L+    KPRRTI    W AEE GLIG+  ++ ++
Sbjct: 356 QRLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEEN 394


>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
           protein similar to PF02225: PA domain; similar to
           N-acetylated-alpha-linked acidic dipeptidase II
           (NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
           Glutamate carboxypeptidase II (Membrane glutamate
           carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
           acidic dipeptidase I) (NAALADase I)
           (Pteroylpoly-gamma-glutamate
           carboxypeptidase)(Folylpoly-gamma-glutamate
           carboxypeptidase) (FGCP) (Folate hydrolase 1)
           (Prostate-specific membrane antigen homolog) (SP|O77564)
           {Sus scrofa}
          Length = 280

 Score = 40.7 bits (91), Expect = 0.002
 Identities = 34/135 (25%), Positives = 66/135 (48%), Gaps = 14/135 (10%)

Query: 158 AQDYENNVTKIPTAAITHEDADLFRR--LQNLGEKIVLKIHMSYAMSRKISRNTIIDLKG 215
           A +   +V  IP+  ++  DA++  +  + ++G    + +++SY +++   +N I  ++G
Sbjct: 117 AVELSGDVPLIPSLPVSAADAEVILKTIVGDVGPGPGI-LNLSYIVTK--IQNVIGVIEG 173

Query: 216 KESPEKQVIVSGHIDSWXXXXXXXXXXXXXXI----SWFVPVILNILKL-----KPRRTI 266
           +E P++ VI+  H D+W              +    S+   +   + KL     KPRRTI
Sbjct: 174 EEEPDRYVILRNHRDTWTFRAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPRRTI 233

Query: 267 RAILWTAEEPGLIGA 281
               W AEE GL+ +
Sbjct: 234 ILCNWDAEEYGLVSS 248


>At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransferase,
           putative similar to polynucleotide phosphorylase
           GB:AAC50039 [Pisum sativum], identical to putative
           polynucleotide phosphorylase GB:AAF00646 [Arabidopsis
           thaliana]
          Length = 922

 Score = 33.5 bits (73), Expect = 0.26
 Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 1/73 (1%)

Query: 252 PVILNILKLKPRRTIRAILWTAEEPGLIGAEAYLTKHINETSNINFIMESDEGTFKPLGL 311
           P  L++ K  P   I  +   ++   LIG+     K I E S +  I   D+GT K + +
Sbjct: 682 PPTLSLSKYAPLILIMKV-HPSKVYSLIGSGGKKVKSIIEESGVEAIDMQDDGTVKIMAI 740

Query: 312 DVAGSTNARCIIA 324
           DVA    A+ II+
Sbjct: 741 DVASLERAKAIIS 753


>At1g30680.1 68414.m03751 toprim domain-containing protein contains
           Pfam profile: PF01751 toprim domain
          Length = 709

 Score = 29.5 bits (63), Expect = 4.2
 Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 4/82 (4%)

Query: 114 PPFVSYGETVVYRSLG-ASKASRKGAVATLVRSITPFSIYSLHTGAQDYENNVTKIPTAA 172
           P F SY  +  Y S    S  SR+       R ++  S Y   T      N++ ++PT  
Sbjct: 35  PSFASYPSSPSYSSSRQVSSVSRRFRPVLASRPVSKNSPYYQRTNGLSSYNSIPRVPTPV 94

Query: 173 ITHEDAD---LFRRLQNLGEKI 191
            T  +AD   +  RL  L  K+
Sbjct: 95  DTEVEADKRVVLSRLVTLRRKL 116


>At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase,
           putative similar to N-carbamoyl-L-amino acid hydrolase
           [Bacillus stearothermophilus] SWISS-PROT:Q53389
          Length = 476

 Score = 28.3 bits (60), Expect = 9.8
 Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 3/29 (10%)

Query: 255 LNILK---LKPRRTIRAILWTAEEPGLIG 280
           +N+LK    KP+R++  IL+T+EEP   G
Sbjct: 161 INVLKRSGFKPKRSLEIILFTSEEPTRFG 189


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.317    0.134    0.387 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,055,756
Number of Sequences: 28952
Number of extensions: 362439
Number of successful extensions: 888
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 881
Number of HSP's gapped (non-prelim): 6
length of query: 409
length of database: 12,070,560
effective HSP length: 83
effective length of query: 326
effective length of database: 9,667,544
effective search space: 3151619344
effective search space used: 3151619344
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 60 (28.3 bits)

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