BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001275-TA|BGIBMGA001275-PA|IPR002052|N-6 Adenine-specific DNA methylase, IPR007484|Peptidase M28 (409 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g54720.1 68416.m06054 glutamate carboxypeptidase, putative (... 46 3e-05 At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept... 42 6e-04 At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 41 0.002 At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransfera... 33 0.26 At1g30680.1 68414.m03751 toprim domain-containing protein contai... 29 4.2 At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase, puta... 28 9.8 >At3g54720.1 68416.m06054 glutamate carboxypeptidase, putative (AMP1) identical to GI:15624092 glutamate carboxypeptidase {Arabidopsis thaliana}; ileal peptidase, Rattus norvegicus, EMBL:AF009921; identical to cDNA glutamate carboxypeptidase (AMP1) GI:15624091; contains Pfam profiles PF04389: Peptidase family M28, PF04253: Transferrin receptor-like dimerisation domain and PF02225 PA domain Length = 705 Score = 46.4 bits (105), Expect = 3e-05 Identities = 26/102 (25%), Positives = 48/102 (47%), Gaps = 3/102 (2%) Query: 190 KIVLKIHMSYAMSRKISRNTIIDLKGKESPEKQVIVSGHIDSWXXXXXXXXXXXXXXISW 249 ++V+ + M K N ++ ++G E ++ VI+ H D+W + Sbjct: 318 RMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAVDPNSGTSALLDI 377 Query: 250 FVPVILNILK--LKPRRTIRAILWTAEEPGLIGAEAYLTKHI 289 L +LK +PRRTI W AEE G+IG+ ++ +++ Sbjct: 378 SRRFAL-LLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENV 418 >At5g19740.1 68418.m02347 peptidase M28 family protein ileal peptidase I100 - Rattus norvegicus, EMBL:AF009921; contains Pfam profiles PF04389: Peptidase family M28, PF02225: PA domain Length = 681 Score = 42.3 bits (95), Expect = 6e-04 Identities = 29/99 (29%), Positives = 50/99 (50%), Gaps = 7/99 (7%) Query: 195 IHMSYAMSRKISR--NTIIDLKGKESPEKQVIVSGHIDSWXXXXXXXXXXXXXXISWFVP 252 +++SY I++ N I ++G+E P++ VI+ H D+W + + Sbjct: 298 LNLSYIGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAVLME--IA 355 Query: 253 VILNILKL---KPRRTIRAILWTAEEPGLIGAEAYLTKH 288 L+ L+ KPRRTI W AEE GLIG+ ++ ++ Sbjct: 356 QRLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEEN 394 >At4g07670.1 68417.m01203 protease-associated (PA) domain-containing protein similar to PF02225: PA domain; similar to N-acetylated-alpha-linked acidic dipeptidase II (NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to Glutamate carboxypeptidase II (Membrane glutamate carboxypeptidase) (mGCP) (N-acetylated-alpha-linked acidic dipeptidase I) (NAALADase I) (Pteroylpoly-gamma-glutamate carboxypeptidase)(Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Folate hydrolase 1) (Prostate-specific membrane antigen homolog) (SP|O77564) {Sus scrofa} Length = 280 Score = 40.7 bits (91), Expect = 0.002 Identities = 34/135 (25%), Positives = 66/135 (48%), Gaps = 14/135 (10%) Query: 158 AQDYENNVTKIPTAAITHEDADLFRR--LQNLGEKIVLKIHMSYAMSRKISRNTIIDLKG 215 A + +V IP+ ++ DA++ + + ++G + +++SY +++ +N I ++G Sbjct: 117 AVELSGDVPLIPSLPVSAADAEVILKTIVGDVGPGPGI-LNLSYIVTK--IQNVIGVIEG 173 Query: 216 KESPEKQVIVSGHIDSWXXXXXXXXXXXXXXI----SWFVPVILNILKL-----KPRRTI 266 +E P++ VI+ H D+W + S+ + + KL KPRRTI Sbjct: 174 EEEPDRYVILRNHRDTWTFRAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPRRTI 233 Query: 267 RAILWTAEEPGLIGA 281 W AEE GL+ + Sbjct: 234 ILCNWDAEEYGLVSS 248 >At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransferase, putative similar to polynucleotide phosphorylase GB:AAC50039 [Pisum sativum], identical to putative polynucleotide phosphorylase GB:AAF00646 [Arabidopsis thaliana] Length = 922 Score = 33.5 bits (73), Expect = 0.26 Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 1/73 (1%) Query: 252 PVILNILKLKPRRTIRAILWTAEEPGLIGAEAYLTKHINETSNINFIMESDEGTFKPLGL 311 P L++ K P I + ++ LIG+ K I E S + I D+GT K + + Sbjct: 682 PPTLSLSKYAPLILIMKV-HPSKVYSLIGSGGKKVKSIIEESGVEAIDMQDDGTVKIMAI 740 Query: 312 DVAGSTNARCIIA 324 DVA A+ II+ Sbjct: 741 DVASLERAKAIIS 753 >At1g30680.1 68414.m03751 toprim domain-containing protein contains Pfam profile: PF01751 toprim domain Length = 709 Score = 29.5 bits (63), Expect = 4.2 Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 4/82 (4%) Query: 114 PPFVSYGETVVYRSLG-ASKASRKGAVATLVRSITPFSIYSLHTGAQDYENNVTKIPTAA 172 P F SY + Y S S SR+ R ++ S Y T N++ ++PT Sbjct: 35 PSFASYPSSPSYSSSRQVSSVSRRFRPVLASRPVSKNSPYYQRTNGLSSYNSIPRVPTPV 94 Query: 173 ITHEDAD---LFRRLQNLGEKI 191 T +AD + RL L K+ Sbjct: 95 DTEVEADKRVVLSRLVTLRRKL 116 >At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase, putative similar to N-carbamoyl-L-amino acid hydrolase [Bacillus stearothermophilus] SWISS-PROT:Q53389 Length = 476 Score = 28.3 bits (60), Expect = 9.8 Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 3/29 (10%) Query: 255 LNILK---LKPRRTIRAILWTAEEPGLIG 280 +N+LK KP+R++ IL+T+EEP G Sbjct: 161 INVLKRSGFKPKRSLEIILFTSEEPTRFG 189 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.317 0.134 0.387 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,055,756 Number of Sequences: 28952 Number of extensions: 362439 Number of successful extensions: 888 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 881 Number of HSP's gapped (non-prelim): 6 length of query: 409 length of database: 12,070,560 effective HSP length: 83 effective length of query: 326 effective length of database: 9,667,544 effective search space: 3151619344 effective search space used: 3151619344 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 60 (28.3 bits)
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