Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001274-TA|BGIBMGA001274-PA|IPR003137|Protease-associated
PA, IPR007484|Peptidase M28
(472 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g54720.1 68416.m06054 glutamate carboxypeptidase, putative (... 53 4e-07
At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept... 49 8e-06
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 38 0.014
At2g44980.2 68415.m05601 transcription regulatory protein SNF2, ... 32 0.72
At1g76970.1 68414.m08962 VHS domain-containing protein / GAT dom... 30 3.8
At3g26700.1 68416.m03339 protein kinase family protein contains ... 29 5.0
At5g35170.1 68418.m04168 adenylate kinase family protein contain... 29 6.7
At1g55160.1 68414.m06299 expressed protein 29 6.7
>At3g54720.1 68416.m06054 glutamate carboxypeptidase, putative
(AMP1) identical to GI:15624092 glutamate
carboxypeptidase {Arabidopsis thaliana}; ileal
peptidase, Rattus norvegicus, EMBL:AF009921; identical
to cDNA glutamate carboxypeptidase (AMP1) GI:15624091;
contains Pfam profiles PF04389: Peptidase family M28,
PF04253: Transferrin receptor-like dimerisation domain
and PF02225 PA domain
Length = 705
Score = 53.2 bits (122), Expect = 4e-07
Identities = 43/166 (25%), Positives = 77/166 (46%), Gaps = 9/166 (5%)
Query: 253 IVLNIKMSSTLDTKVSRNTIIDLKGSVHPEKLVIVSGHIDSWDVGQGAMDDGGG----LF 308
+V+N+ + K N ++ ++GS ++ VI+ H D+W GA+D G L
Sbjct: 319 MVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWT--YGAVDPNSGTSALLD 376
Query: 309 ISWAAPVILKRLNLIPKRTVRSIFWTAEELGLVGAYAY-EEAHRNESHNINFIMESDEGT 367
IS ++LK P+RT+ W AEE G++G+ + EE N + + D
Sbjct: 377 ISRRFALLLKS-GWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASAVAYLNVDCAV 435
Query: 368 FAPLGLIVAGTDKARCIVAEVLKLFESINASTLIEDDSPGSDISVI 413
G T + ++ +VLKL + +A L +++ S ++I
Sbjct: 436 QGS-GFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNII 480
>At5g19740.1 68418.m02347 peptidase M28 family protein ileal
peptidase I100 - Rattus norvegicus, EMBL:AF009921;
contains Pfam profiles PF04389: Peptidase family M28,
PF02225: PA domain
Length = 681
Score = 48.8 bits (111), Expect = 8e-06
Identities = 44/166 (26%), Positives = 76/166 (45%), Gaps = 6/166 (3%)
Query: 254 VLNIK-MSSTLDTKVSRNTIIDLKGSVHPEKLVIVSGHIDSWDVGQGAMDDGGGLFISWA 312
VLN+ + T+ K+ N I ++G P++ VI+ H D+W G + G + + A
Sbjct: 297 VLNLSYIGETVIAKIE-NVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAVLMEIA 355
Query: 313 APVI-LKRLNLIPKRTVRSIFWTAEELGLVGAYAYEEAHRNE-SHNINFIMESDEGTFAP 370
+ L++ P+RT+ W AEE GL+G+ + E +R S + D P
Sbjct: 356 QRLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLSSRAVAYLNVDCAVSGP 415
Query: 371 LGLIVAGTDKA-RCIVAEVLKLFESINASTLIEDDSPGSDISVIIK 415
G + T + I ++ + NA+ I + GS SV+I+
Sbjct: 416 -GFHASATPQLDELIKVAAQEVRDPDNATQTIYESWIGSSDSVVIR 460
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 37.9 bits (84), Expect = 0.014
Identities = 35/125 (28%), Positives = 58/125 (46%), Gaps = 14/125 (11%)
Query: 230 IPTAAITLEDADLIRRMYDRGENIVLNIKMSSTLDTKVSRNTIIDLKGSVHPEKLVIVSG 289
IP+ ++ DA++I + I S + TK+ +N I ++G P++ VI+
Sbjct: 127 IPSLPVSAADAEVILKTIVGDVGPGPGILNLSYIVTKI-QNVIGVIEGEEEPDRYVILRN 185
Query: 290 HIDSWDVGQGAMDDGGGLFISWAAPV-----ILKRLNLI------PKRTVRSIFWTAEEL 338
H D+W A+D G + A I +RL+ + P+RT+ W AEE
Sbjct: 186 HRDTWTF--RAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPRRTIILCNWDAEEY 243
Query: 339 GLVGA 343
GLV +
Sbjct: 244 GLVSS 248
>At2g44980.2 68415.m05601 transcription regulatory protein SNF2,
putative similar to SNF2P [Oryza sativa (japonica
cultivar-group)] GI:23193483; contains Pfam profiles
PF00271: Helicase conserved C-terminal domain, PF00176:
SNF2 family N-terminal domain; CG donor site annotated
in one isoform based on protein alignments.
Length = 870
Score = 32.3 bits (70), Expect = 0.72
Identities = 40/134 (29%), Positives = 62/134 (46%), Gaps = 17/134 (12%)
Query: 242 LIRRMYDRGENIVLNIKMSSTLD-----TKVSRNTIIDLKGSVH-PEKLVIVSGHIDSWD 295
L++R++D G ++L +M+STLD ++ R + L GSV E+ + +
Sbjct: 385 LLKRLHDSGHRVLLFSQMTSTLDILQDFMELRRYSYERLDGSVRAEERFAAIKNFSAKTE 444
Query: 296 VGQGAMDDGGGLF---ISWAAPVILKRLNLIPKRTVRSIFWTAEELGLVGAYAYEEAHR- 351
G + DG F IS A + LNL+ TV IF+ + V A + AHR
Sbjct: 445 RGLDSEVDGSNAFVFMISTRAGGV--GLNLVAADTV--IFYEQDWNPQVDKQALQRAHRI 500
Query: 352 -NESH--NINFIME 362
SH +IN + E
Sbjct: 501 GQISHVLSINLVTE 514
>At1g76970.1 68414.m08962 VHS domain-containing protein / GAT
domain-containing protein weak similarity to
HGF-regulated tyrosine kinase substrate [Mus musculus]
GI:1089781; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 446
Score = 29.9 bits (64), Expect = 3.8
Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Query: 394 SINASTLIEDDSPG-SDISVIIKTGIPGASLHNENEKYFWFHHT 436
S ++S + +D SP S S +I+ P S HN+ +++F HH+
Sbjct: 349 SSSSSPVFDDASPQQSKSSEVIRNLPPPPSRHNQRQQFFEHHHS 392
>At3g26700.1 68416.m03339 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 380
Score = 29.5 bits (63), Expect = 5.0
Identities = 17/56 (30%), Positives = 27/56 (48%)
Query: 76 ARPSGSEVLERSIDYMIELTREEDINDIVTEELEVPHWVRGEEKITMLEPRVKDIS 131
+ PS S + +D+M LT DI ++ E L + G E++ L R D+S
Sbjct: 279 SEPSPSSSTQTLVDWMQNLTDYADIPMMIDERLGGTYTAEGVEELITLTLRCVDVS 334
>At5g35170.1 68418.m04168 adenylate kinase family protein contains
Pfam profile: PF00406 adenylate kinase
Length = 588
Score = 29.1 bits (62), Expect = 6.7
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 8/82 (9%)
Query: 49 EILNYVLQGDFKGQTYDQLAKFVDKFGARPSGSEVLERSIDYMIELTREEDINDIVTEEL 108
+IL Y+ GD + L FV K GA G EV E D I + + + + + ++L
Sbjct: 406 KILEYMDWGDD-----ETLGTFV-KLGAI-GGKEVDEED-DMFILVAPQNAVGNCIIDDL 457
Query: 109 EVPHWVRGEEKITMLEPRVKDI 130
+ G+ + ++ PR+KD+
Sbjct: 458 QAMTTAAGKRPVVLINPRLKDL 479
>At1g55160.1 68414.m06299 expressed protein
Length = 188
Score = 29.1 bits (62), Expect = 6.7
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Query: 312 AAPVILKRLNLIPKRTVRSIF-WTAEELGLVGAYAYEEAHRNE 353
A PV++K IP++ R +F W EE V EE RN+
Sbjct: 128 AEPVVIKAREPIPEKQQRELFKWMLEEKRKVNPKNAEEKKRND 170
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.135 0.392
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,753,903
Number of Sequences: 28952
Number of extensions: 462750
Number of successful extensions: 1050
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1044
Number of HSP's gapped (non-prelim): 8
length of query: 472
length of database: 12,070,560
effective HSP length: 84
effective length of query: 388
effective length of database: 9,638,592
effective search space: 3739773696
effective search space used: 3739773696
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 61 (28.7 bits)
- SilkBase 1999-2023 -