BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001268-TA|BGIBMGA001268-PA|IPR011009|Protein kinase-like, IPR008973|C2 calcium/lipid-binding region, CaLB, IPR000403|Phosphatidylinositol 3- and 4-kinase, catalytic, IPR000341|Phosphoinositide 3-kinase, ras-binding, IPR002420|Phosphoinositide 3-kinase, C2, IPR001263|Phosphoinositide 3-kinase accessory region PIK, IPR001683|Phox-like (1505 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizo... 139 3e-33 SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 120 1e-27 SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosacc... 93 3e-19 SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 50 3e-06 SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 46 3e-05 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 46 5e-05 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 46 5e-05 SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 31 0.93 >SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizosaccharomyces pombe|chr 1|||Manual Length = 801 Score = 139 bits (336), Expect = 3e-33 Identities = 136/505 (26%), Positives = 218/505 (43%), Gaps = 46/505 (9%) Query: 882 QSQLLHLIEQGAYQRMPTECREVMWEKRQYLVQIPGALPLVLLAATNWYGDHREQLIGLL 941 + +L ++ + + E ++++W+ R YL + A+ L + Q + LL Sbjct: 267 RKELESILSYPPSEELSLEEKDLIWKFRFYLTRNKKAMTKFLKSVVWTDSSEVNQALSLL 326 Query: 942 RLWEKPSPLNAMHLLLPCFPDVAVRETAAKLLNEMSDDDLCRVLPQLTQALRHETYXXXX 1001 W + +A+ LL P F VR A L S+++L L L Q ++ Y Sbjct: 327 DSWTEIDIDDALELLSPSFVHPKVRAYAVSRLETASNEEL---LLYLLQLVQALRYDNPI 383 Query: 1002 XXXXXXXXXXXXXXXXXXXFWLLVHSLPNIPQAPHQEFLGINLEDNASENAETDASMSAS 1061 S P+I + +L + +ED E + + +S Sbjct: 384 SSDERFQPSPLALFLVNRAI-----SSPSIGNDLYW-YLVVEIED------EPVSKLFSS 431 Query: 1062 WRYTLMLRALLVICGENLNKTLLRQQLLVKSLCEMAVSVKCAKEXXXXXXXXXXXXXXXX 1121 + + G + +TL Q V+ L ++ SV+ + Sbjct: 432 VMFLFQKELSKSVEGRLIRETLSAQAKFVEKLLRISKSVQSFRGTRLKKIEYLKVLLEDH 491 Query: 1122 XXXXQPT-ALPLALHKYVHDLDV--KSCTYFSSNTLPLKINFIGTDNAVIPAMFKVGDDL 1178 ALPL L V+ + + +CT F S PL++ F D + P +FK GDDL Sbjct: 492 KYHLLDFHALPLPLDPSVNIVGIIPDACTVFKSTMQPLRLLFKCQDGSKYPIIFKNGDDL 551 Query: 1179 RQDALVLQVIKIMDTLWLKAGLDLRMVTFQALPTSNSKGMIEIVTEAETLRAIQTEWGLT 1238 RQD LV+Q++ +MD L K LDL + ++ L T + G ++ V ++TL I E+ Sbjct: 552 RQDQLVIQILTLMDKLLKKEKLDLHLKPYRILATGPTHGAVQFVP-SKTLATILAEYH-- 608 Query: 1239 GSFKDKPIAEWLAKHNPSE----LEY---QRARDNFTASCAGYSVATYLLGICDRHNDNI 1291 GS + +L ++NP + Y A DN+ SCAGY V TYLLG+ DRH DN+ Sbjct: 609 GS-----VLAYLRENNPDDGLNSANYGIDPVAMDNYVRSCAGYCVITYLLGVGDRHLDNL 663 Query: 1292 MLKTSGHLFHIDFGKFLG-DAQMFGNFKRLVYIDRAPFVLTHDMVYVINGGERPTQRFQH 1350 ++ GH FH DFG LG D ++F + L+ +MV + G P +Q Sbjct: 664 LITKDGHFFHADFGYILGRDPKLFSPAMK----------LSKEMVEGMGGYNSPF--YQQ 711 Query: 1351 FVELCCMAFNIVRAHHDHILDLFAL 1375 F C F +R + IL+LF+L Sbjct: 712 FKSYCYTTFTALRKSSNLILNLFSL 736 >SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1877 Score = 120 bits (290), Expect = 1e-27 Identities = 77/202 (38%), Positives = 108/202 (53%), Gaps = 16/202 (7%) Query: 1170 AMFKVGDDLRQDALVLQVIKIMDTLWLKAGLDLRMVTFQALPTSNSKGMIEIVTEAETLR 1229 A+FKVGDD RQD L LQ+I + ++ GLD+ + ++ T+ G+I+++ + Sbjct: 1621 AIFKVGDDCRQDVLTLQLIAMFKNIFNSVGLDVYLFPYRVTATNPGCGVIDVLPNCISRD 1680 Query: 1230 AIQTEW--GLTGSFKDKPIAEWLAKHNPSELEYQRARDNFTASCAGYSVATYLLGICDRH 1287 + E GL F+ K E + +Q+AR NF S A YSV TYLL DRH Sbjct: 1681 MLGREAVNGLYDYFRTKFGDE-------DSIAFQKARSNFVQSMAAYSVITYLLQFKDRH 1733 Query: 1288 NDNIMLKTSGHLFHIDFGKFLGDAQMFGNFKRLVYIDRAPFVLTHDMVYVINGGERPTQR 1347 N NIM+ GH+ HIDFG F+ D G + + APF LT +M+ V+ GG +Q Sbjct: 1734 NGNIMIDDQGHILHIDFG-FIFDIAPGG-----ITFESAPFKLTTEMIAVM-GGSNKSQP 1786 Query: 1348 FQHFVELCCMAFNIVRAHHDHI 1369 FQ F ELC AF R + +I Sbjct: 1787 FQWFQELCVKAFLACRPYAHYI 1808 >SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosaccharomyces pombe|chr 1|||Manual Length = 851 Score = 93.1 bits (221), Expect = 3e-19 Identities = 65/219 (29%), Positives = 109/219 (49%), Gaps = 29/219 (13%) Query: 1167 VIPAMFKVGDDLRQDALVLQVIKIMDTLWLKAGLDLRMVTFQALPTSNSKGMIEIVTEAE 1226 ++ + K G DLRQ+ Q+I +WL+ + + + L T ++ G+IE +T A Sbjct: 578 LVSVIVKTGADLRQETFACQLIYAFQRVWLECKEKVWVRRMKILVTGDNSGLIETITNAI 637 Query: 1227 TLRAIQTEWGLTGSFKDKPIAEW-LAKHN--------------PSELEYQRARDNFTASC 1271 ++ +I+ LT ++ +A+ +A N P+ Y++A+ NF S Sbjct: 638 SVHSIKKN--LTKQLREAELAQGKIAGKNVVTLKDYFIKQFGDPNSSRYRQAQTNFLQSL 695 Query: 1272 AGYSVATYLLGICDRHNDNIMLKTSGHLFHIDFGKFLGDAQMFGNFKRLVYIDRAPFVLT 1331 YS+ +YLL + DRHN N+++ + GH+ HIDFG L + GN V + APF LT Sbjct: 696 VAYSIISYLLQLKDRHNGNVLIDSEGHIIHIDFGFLLTNTP--GN----VGFESAPFKLT 749 Query: 1332 HDMVYVINGGERPTQRFQHFVELCCMAFNIVRAHHDHIL 1370 D + +++ RF + L AF VR + D I+ Sbjct: 750 ADYLEILD------DRFDEYRSLMKAAFKSVRKNADQII 782 >SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 2386 Score = 49.6 bits (113), Expect = 3e-06 Identities = 27/89 (30%), Positives = 43/89 (48%), Gaps = 7/89 (7%) Query: 1248 EWLAKHNPSELEYQRARDNFTASCAGYSVATYLLGICDRHNDNIML-KTSGHLFHIDFGK 1306 EW + P + +R N+ + A S+ Y+LG+ DRH +NI+ + +G H+DF Sbjct: 2193 EWFVESFPEPNNWVTSRQNYCRTLAVMSIVGYVLGLGDRHGENILFDEFTGEAIHVDFNC 2252 Query: 1307 FLGDAQMFGNFKRLVYIDRAPFVLTHDMV 1335 F ++ PF LTH+MV Sbjct: 2253 LFDKGLTFEK------PEKVPFRLTHNMV 2275 Score = 34.3 bits (75), Expect = 0.13 Identities = 24/81 (29%), Positives = 38/81 (46%), Gaps = 4/81 (4%) Query: 1155 PLKINFIGTDNAVIPAMFKVGDDLRQDALVLQ----VIKIMDTLWLKAGLDLRMVTFQAL 1210 P K+ GTD + P + K DDLR+DA +++ + KI+ +L + T+ + Sbjct: 2065 PRKVYVRGTDGNLYPFLCKPKDDLRKDARLMEFNNLICKILRKDQEANRRNLCIRTYVVI 2124 Query: 1211 PTSNSKGMIEIVTEAETLRAI 1231 P + G IE V R I Sbjct: 2125 PLNEECGFIEWVNHTRPFREI 2145 >SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 2812 Score = 46.4 bits (105), Expect = 3e-05 Identities = 29/79 (36%), Positives = 43/79 (54%), Gaps = 7/79 (8%) Query: 1258 LEYQRARDNFTASCAGYSVATYLLGICDRHNDNIML-KTSGHLFHIDFGKFLGDAQMFGN 1316 +++ + N+ S A SV ++LG+ DRH NI++ KTSG + HID G F Sbjct: 2603 VQWFTTQTNYARSTAVASVLGHVLGLGDRHGQNILIDKTSGEVIHIDLG------IAFEQ 2656 Query: 1317 FKRLVYIDRAPFVLTHDMV 1335 K+L + PF LT D+V Sbjct: 2657 GKKLPVPECVPFRLTRDVV 2675 Score = 30.7 bits (66), Expect = 1.6 Identities = 23/82 (28%), Positives = 41/82 (50%), Gaps = 5/82 (6%) Query: 1148 YFSSNTLPLKINFIGTDNAVIPAMFKVG-DDLRQDALVLQVIK----IMDTLWLKAGLDL 1202 + S P I +G++ + K G DDLRQDA++ QV + + + + +L Sbjct: 2455 FASGINAPKVITCLGSNGHTYKQLVKGGNDDLRQDAVMEQVFEQVNGFLRSYRKTSQRNL 2514 Query: 1203 RMVTFQALPTSNSKGMIEIVTE 1224 M T++ +P + G+IE V + Sbjct: 2515 SMRTYKVIPLALKTGVIEWVQD 2536 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 45.6 bits (103), Expect = 5e-05 Identities = 32/88 (36%), Positives = 47/88 (53%), Gaps = 8/88 (9%) Query: 1249 WLAKHNPSELEYQRARDNFTASCAGYSVATYLLGICDRHNDNIML-KTSGHLFHIDFGKF 1307 WL K SE R R N++ + A S+ Y+LG+ DRH N+ML + +G++ HIDFG Sbjct: 2106 WL-KSRSSEAWLNR-RTNYSRTLAVMSMVGYILGLGDRHPSNLMLDRYTGNIIHIDFGDC 2163 Query: 1308 LGDAQMFGNFKRLVYIDRAPFVLTHDMV 1335 A R + ++ PF LT +V Sbjct: 2164 FEVA-----MHREKFPEKIPFRLTRMLV 2186 Score = 31.9 bits (69), Expect = 0.70 Identities = 21/86 (24%), Positives = 43/86 (50%), Gaps = 4/86 (4%) Query: 1150 SSNTLPLKINFIGTDNAVIPAMFKVGDDLRQDALVLQVIKIMDTLWLKAGLDL-RMVTFQ 1208 +S P +++ G+D + K +D+RQD V+Q+ + + L L R+++ Q Sbjct: 1966 TSKQRPRRLSIKGSDGKDYQYVLKGHEDIRQDERVMQLFGLCNNLLLADPETFKRLLSIQ 2025 Query: 1209 ---ALPTSNSKGMIEIVTEAETLRAI 1231 +P S G++ V +++TL + Sbjct: 2026 RYPVIPLSPDSGLLGWVLDSDTLHVL 2051 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 45.6 bits (103), Expect = 5e-05 Identities = 26/58 (44%), Positives = 35/58 (60%), Gaps = 3/58 (5%) Query: 1249 WLAKHNPSELEYQRARDNFTASCAGYSVATYLLGICDRHNDNIML-KTSGHLFHIDFG 1305 WL K SE R R ++T S A S+ Y+LG+ DRH N+M+ + SG + HIDFG Sbjct: 2103 WL-KSRSSEAWLDR-RTSYTQSLAVMSMVGYILGLGDRHPSNLMMDRYSGKIIHIDFG 2158 Score = 33.5 bits (73), Expect = 0.23 Identities = 23/90 (25%), Positives = 42/90 (46%), Gaps = 4/90 (4%) Query: 1150 SSNTLPLKINFIGTDNAVIPAMFKVGDDLRQDALVLQVIKIMDTLWLKAG----LDLRMV 1205 SS P ++ G+D + K +DLRQD V+Q+ + +TL L + Sbjct: 1963 SSKQRPRRLTIHGSDGKDYQYVLKGHEDLRQDERVMQLFGLCNTLLTTDSETFKRRLNIE 2022 Query: 1206 TFQALPTSNSKGMIEIVTEAETLRAIQTEW 1235 + +P S + G++ V ++TL + E+ Sbjct: 2023 RYTVIPLSPNSGLLGWVPHSDTLHFLIKEF 2052 >SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccharomyces pombe|chr 3|||Manual Length = 721 Score = 31.5 bits (68), Expect = 0.93 Identities = 15/39 (38%), Positives = 20/39 (51%) Query: 276 PLLTEQSNPEYAVGSECSSSVYEEYDPLDFLYGETEMRE 314 P+L N E+A +S+Y + D LDF G E RE Sbjct: 218 PVLVFNPNKEFAREDAAITSIYYDNDDLDFYLGRLEKRE 256 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,142,226 Number of Sequences: 5004 Number of extensions: 239163 Number of successful extensions: 588 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 565 Number of HSP's gapped (non-prelim): 15 length of query: 1505 length of database: 2,362,478 effective HSP length: 82 effective length of query: 1423 effective length of database: 1,952,150 effective search space: 2777909450 effective search space used: 2777909450 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 60 (28.3 bits)
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