BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001241-TA|BGIBMGA001241-PA|IPR001404|Heat shock protein Hsp90, IPR003594|ATP-binding region, ATPase-like, IPR009079|Four-helical cytokine (658 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 220 6e-58 SPAC1006.03c |||human CCDC131 homolog|Schizosaccharomyces pombe|... 31 0.67 SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma c... 29 1.5 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 29 1.5 SPCC613.11c |meu23|B13958-2|DUF1773 family protein 2|Schizosacch... 29 1.5 SPBC1604.16c |||RNA-binding protein, G-patch type |Schizosacchar... 29 2.7 SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr 1|... 27 6.3 SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 27 6.3 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 27 6.3 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 27 8.3 SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 27 8.3 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 8.3 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 220 bits (537), Expect = 6e-58 Identities = 131/414 (31%), Positives = 221/414 (53%), Gaps = 17/414 (4%) Query: 247 DQVNSIKPLWLFEPKEITQEQHIEFYKFISNSYDKPRFTLHYKTDAPLSIRSILYVPEGK 306 +++N KP+W P E+T+E++ FYK ++N ++ H+ + L R+IL+VP Sbjct: 263 EELNKTKPIWTRNPSEVTKEEYASFYKSLTNDWEDHLAVKHFSVEGQLEFRAILFVPRRA 322 Query: 307 P-GLFELSRDSDVGVALYSRKILIKSKAENILPKWLRFVKGVVDSEDIPLNLSRELLQNS 365 P LFE R + + LY R++ I E ++P+WL F+KGVVDSED+PLNLSRE+LQ + Sbjct: 323 PMDLFEAKRKKN-NIKLYVRRVFITDDCEELIPEWLGFIKGVVDSEDLPLNLSREMLQQN 381 Query: 366 ALITKLRTVLTNRFLKFMVEMSQKDPLQYEAFYKDYSIFLKEGIVTSQSPLEKEEIAKLL 425 ++ +R L R L E+++ D ++ FY +S LK GI + + +AKLL Sbjct: 382 KIMKVIRKNLVRRCLDMFNEIAE-DKENFKTFYDAFSKNLKLGI--HEDAANRPALAKLL 438 Query: 426 RFESSKLSNGQRTSLSDYSSRQKDDQKSIFYLAAPSRELAETSPYYESLKKRDIEVLFCY 485 R+ S S SL DY ++ + QK+I+++ S++ E SP+ E + + +VLF Sbjct: 439 RYNSLN-SPDDLISLEDYITKMPEHQKNIYFITGESKQAVENSPFLEIFRAKKFDVLFMV 497 Query: 486 EMYDELVLLELKEFGGRALVSVEND-MQRDPLDKSESIIGSEGLQQSQVDELISFLKTNL 544 + DE + +LKEF G+ LV++ D ++ + D+ ++ E L++ + +E LKT L Sbjct: 498 DPIDEYAVTQLKEFEGKKLVNITKDGLELEETDEEKA--AREKLEK-EYEEFAKQLKTIL 554 Query: 545 TGKVFDVRTTQKLDSHPCVIVVPE---MAAARHFIRTQAQNLTEENRFALLRPQLEINAK 601 KV V + K+ PC++ + A ++ QA T + + R EIN K Sbjct: 555 GDKVEKVVVSNKIVGSPCLLTTGQYGWSANMERIMKAQALRDTSMSAYMSSRKTFEINPK 614 Query: 602 HPIVKKLHKLM---STDKELATMVAQQLFSNAMVTAGLVM-DPRNLITHINDLL 651 PI+ +L K + + +A L+ A++++G + DP IN L+ Sbjct: 615 SPIIAELKKKVEENGAEDRSVKDLATILYETALLSSGFTLDDPSAYAQRINRLI 668 Score = 179 bits (436), Expect = 1e-45 Identities = 94/214 (43%), Positives = 145/214 (67%), Gaps = 12/214 (5%) Query: 34 SEKREFQAETRMLLDIVARSLYSDKEVFIRELISNASDALEKFRYLSVSSVPESGNLKEL 93 +E +F+AE L+ ++ ++YS+KE+F+RELISNASDAL+K RY S+S P + + Sbjct: 4 TETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQSLSD-PHA---LDA 59 Query: 94 DRPLEIRLTTDKQNRTITFQDSGIGMTKEELTQNLGTIARSGSKSFIEEIKKQGAEQASS 153 ++ L IR+T DK+N+ ++ +D+GIGMTK +L NLG IA+SG+K F+ E GA+ S Sbjct: 60 EKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFM-EAAASGAD--IS 116 Query: 154 IIGQFGVGFYSAFMVADKIEVFTRSSKGDSPGYQWSSDGSGTYEIQ-EADGVEI--GTKI 210 +IGQFGVGFYSA++VADK++V S D Y W S G++ + + DG + GT+I Sbjct: 117 MIGQFGVGFYSAYLVADKVQVV--SKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEI 174 Query: 211 VVYLKTDCREFADDDTVKSIIKKYSNFVSSPIFL 244 +++K D ++ ++ T+K +KK+S F+S PI L Sbjct: 175 RLFMKEDQLQYLEEKTIKDTVKKHSEFISYPIQL 208 >SPAC1006.03c |||human CCDC131 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 714 Score = 30.7 bits (66), Expect = 0.67 Identities = 36/148 (24%), Positives = 64/148 (43%), Gaps = 7/148 (4%) Query: 121 KEELTQNLGTIARSGSKSFIEEIKKQGAEQASSIIGQFGVGFYSAFMVADKIE---VFTR 177 K E + L T +++ ++ + ++ AE+A S + + GV F +A+ IE V T Sbjct: 172 KSEKSPFLST-SKNSDANYSKTTNQKEAEKAVSQLFEVGVRFND--FIAEGIEPSVVHTL 228 Query: 178 SSKGDSPGYQWSSDGSGTYEIQEADGVEIGTKIVVYLKTDCREFADDDTVKSIIKKYSNF 237 K SS GS T +A KI L +D D+ +++ + N Sbjct: 229 FLKLGLDSSSASSQGSLTLSADKAARSAKLRKIDSNL-SDTHILPGDNGTPTVLPERKNL 287 Query: 238 VSSPIFLNNDQVNSIKPLWLFEPKEITQ 265 +S P+ +D ++S KP P + + Sbjct: 288 ISLPLLKQDDWLSSSKPFGSSTPNVVIE 315 >SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma catalytic subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 1018 Score = 29.5 bits (63), Expect = 1.5 Identities = 13/46 (28%), Positives = 24/46 (52%) Query: 213 YLKTDCREFADDDTVKSIIKKYSNFVSSPIFLNNDQVNSIKPLWLF 258 Y+++ E ++DD S Y N++ +L + VNS+K + F Sbjct: 274 YIRSQSTETSEDDDSSSFDDDYQNYLKQEPWLAHSSVNSLKDVAKF 319 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 29.5 bits (63), Expect = 1.5 Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 1/64 (1%) Query: 11 KLKEFQHFSTVTEKQSSESLKGKSEKREFQAETRMLLDIVARSLYSDKEVF-IRELISNA 69 K +E +H T+K +E++ K+ E+ +TR LD + +++E +E+++ A Sbjct: 558 KYREAEHQMIATDKLVAETVDRKNALEEYIYDTRAKLDDIYAPFTNEEESSKFKEMLTKA 617 Query: 70 SDAL 73 D L Sbjct: 618 EDWL 621 >SPCC613.11c |meu23|B13958-2|DUF1773 family protein 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 254 Score = 29.5 bits (63), Expect = 1.5 Identities = 26/110 (23%), Positives = 54/110 (49%), Gaps = 5/110 (4%) Query: 35 EKREFQAETRMLLDIVARSLYSDKEVFIRELISNASDALEKF-RYLSVSSVPESGNLKEL 93 EKR +A R +L+ R+L + + E+ +N + +++F R + + E ++ Sbjct: 41 EKRRIEARNR-ILESEGRALENKTHLNEEEIRTN--ERVKRFQRRVDEWTALEIERESQV 97 Query: 94 DRPLEIRLTTDKQNRTITFQDSGIGMTKEELTQNLGTI-ARSGSKSFIEE 142 PL + ++QNRT+ F SG+ + + GT+ G+ + ++E Sbjct: 98 QPPLWYQRDREEQNRTLNFLMSGMADLQRTINTMQGTMNTMQGTMNTMQE 147 >SPBC1604.16c |||RNA-binding protein, G-patch type |Schizosaccharomyces pombe|chr 2|||Manual Length = 199 Score = 28.7 bits (61), Expect = 2.7 Identities = 16/64 (25%), Positives = 28/64 (43%) Query: 123 ELTQNLGTIARSGSKSFIEEIKKQGAEQASSIIGQFGVGFYSAFMVADKIEVFTRSSKGD 182 EL+ + G SF++ +Q ++A+ I FY + D+ T +SK D Sbjct: 8 ELSNSYGFYKNGRRVSFVKATSEQSLDEATFIEKGSAQAFYHSLFENDRDNSHTMNSKRD 67 Query: 183 SPGY 186 G+ Sbjct: 68 EAGF 71 >SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr 1|||Manual Length = 1018 Score = 27.5 bits (58), Expect = 6.3 Identities = 20/59 (33%), Positives = 30/59 (50%), Gaps = 4/59 (6%) Query: 40 QAETRMLLDIVARSLYSDKEVFIRELISNASDALEKFRYLSVSSVPESGNLKELDRPLE 98 Q R+L D+ +S S VF S AS ++E F Y S + +P +L ++ PLE Sbjct: 345 QLSQRLLEDVNVKSCGSCAAVFA----SLASISVETFNYCSCNFLPSVLDLPMVNEPLE 399 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 27.5 bits (58), Expect = 6.3 Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 1/80 (1%) Query: 67 SNASDALEKFRYLSVSSVPESGNLKELDRPLEIRLTTDKQNRTITFQDSGIGMTKEELTQ 126 S SD F +++ +LK + PL R T KQNR + F +++ ++ Sbjct: 39 SRLSDGALNFTGGRIATPLPQPSLKTPESPLSKRNPTIKQNR-VRFDLPDDELSRSNVSS 97 Query: 127 NLGTIARSGSKSFIEEIKKQ 146 T+ S S S + +KK+ Sbjct: 98 PEKTLLTSASTSTFDSLKKE 117 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 27.5 bits (58), Expect = 6.3 Identities = 26/116 (22%), Positives = 51/116 (43%), Gaps = 9/116 (7%) Query: 401 YSIFLKEGIVTSQSPLEKEEIAKLLRFESSKLS----NGQRTSLSDYSSRQKDDQKSI-- 454 Y+ + + S L++ ++A R E K N + + ++ +Q QK + Sbjct: 392 YASLVNKNYEISSGKLKERQVAVRARIEGIKAHEMFLNNRVSEINSSLEKQLTTQKELRS 451 Query: 455 -FYLAAPSRELAETSPYYESLKKRDIEVLFCYEMYDELVLLELKEFGGRALVSVEN 509 F + P + + + + ++K D+ + + Y+ LLEL + AL SVEN Sbjct: 452 RFEILFPVK--LQREDFTKDVEKSDLWIKSLRQEYESKNLLELLDKHQTALSSVEN 505 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 27.1 bits (57), Expect = 8.3 Identities = 14/40 (35%), Positives = 21/40 (52%) Query: 59 EVFIRELISNASDALEKFRYLSVSSVPESGNLKELDRPLE 98 ++ EL+S AS+ L +F V +PES K L +E Sbjct: 407 QIVYPELLSIASERLLRFEDAVVELIPESATAKYLQEDVE 446 >SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1210 Score = 27.1 bits (57), Expect = 8.3 Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 2/34 (5%) Query: 324 SRKILIKSKAENILPKWLRFVKGVVDSEDIPLNL 357 SR +LIK KA+N++ R + ++ SE+I L++ Sbjct: 985 SRSVLIKPKADNVI--LFRLKRFIMSSEEINLDI 1016 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 27.1 bits (57), Expect = 8.3 Identities = 27/102 (26%), Positives = 47/102 (46%), Gaps = 7/102 (6%) Query: 10 SKLKEFQHFSTVTEK-QSSESLKGKSEKREFQAETRMLLDIVARSLYSDKEVFIRELISN 68 SK E H +TE ++ ES+ KS E++ ++ +++RSL +K Sbjct: 526 SKSTEALHSGNLTEHLKTVESILSKSRHEEYRIANHIVAYLLSRSLKQNKTESDNSFAIL 585 Query: 69 ASDALEKFRYLSVSSVPE-SGN---LKELDRPLEIRLTTDKQ 106 LE L++ S+PE G +++ L + +T DKQ Sbjct: 586 TKILLEDL--LNMLSLPEWCGTETIIRQFAMNLVMTVTNDKQ 625 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.133 0.364 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,852,269 Number of Sequences: 5004 Number of extensions: 122217 Number of successful extensions: 427 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 414 Number of HSP's gapped (non-prelim): 14 length of query: 658 length of database: 2,362,478 effective HSP length: 77 effective length of query: 581 effective length of database: 1,977,170 effective search space: 1148735770 effective search space used: 1148735770 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 57 (27.1 bits)
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