SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001241-TA|BGIBMGA001241-PA|IPR001404|Heat shock protein
Hsp90, IPR003594|ATP-binding region, ATPase-like,
IPR009079|Four-helical cytokine
         (658 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom...   220   6e-58
SPAC1006.03c |||human CCDC131 homolog|Schizosaccharomyces pombe|...    31   0.67 
SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma c...    29   1.5  
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    29   1.5  
SPCC613.11c |meu23|B13958-2|DUF1773 family protein 2|Schizosacch...    29   1.5  
SPBC1604.16c |||RNA-binding protein, G-patch type |Schizosacchar...    29   2.7  
SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr 1|...    27   6.3  
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      27   6.3  
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos...    27   6.3  
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|...    27   8.3  
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce...    27   8.3  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    27   8.3  

>SPAC926.04c |hsp90|swo1|heat shock protein
           Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 704

 Score =  220 bits (537), Expect = 6e-58
 Identities = 131/414 (31%), Positives = 221/414 (53%), Gaps = 17/414 (4%)

Query: 247 DQVNSIKPLWLFEPKEITQEQHIEFYKFISNSYDKPRFTLHYKTDAPLSIRSILYVPEGK 306
           +++N  KP+W   P E+T+E++  FYK ++N ++      H+  +  L  R+IL+VP   
Sbjct: 263 EELNKTKPIWTRNPSEVTKEEYASFYKSLTNDWEDHLAVKHFSVEGQLEFRAILFVPRRA 322

Query: 307 P-GLFELSRDSDVGVALYSRKILIKSKAENILPKWLRFVKGVVDSEDIPLNLSRELLQNS 365
           P  LFE  R  +  + LY R++ I    E ++P+WL F+KGVVDSED+PLNLSRE+LQ +
Sbjct: 323 PMDLFEAKRKKN-NIKLYVRRVFITDDCEELIPEWLGFIKGVVDSEDLPLNLSREMLQQN 381

Query: 366 ALITKLRTVLTNRFLKFMVEMSQKDPLQYEAFYKDYSIFLKEGIVTSQSPLEKEEIAKLL 425
            ++  +R  L  R L    E+++ D   ++ FY  +S  LK GI   +    +  +AKLL
Sbjct: 382 KIMKVIRKNLVRRCLDMFNEIAE-DKENFKTFYDAFSKNLKLGI--HEDAANRPALAKLL 438

Query: 426 RFESSKLSNGQRTSLSDYSSRQKDDQKSIFYLAAPSRELAETSPYYESLKKRDIEVLFCY 485
           R+ S   S     SL DY ++  + QK+I+++   S++  E SP+ E  + +  +VLF  
Sbjct: 439 RYNSLN-SPDDLISLEDYITKMPEHQKNIYFITGESKQAVENSPFLEIFRAKKFDVLFMV 497

Query: 486 EMYDELVLLELKEFGGRALVSVEND-MQRDPLDKSESIIGSEGLQQSQVDELISFLKTNL 544
           +  DE  + +LKEF G+ LV++  D ++ +  D+ ++    E L++ + +E    LKT L
Sbjct: 498 DPIDEYAVTQLKEFEGKKLVNITKDGLELEETDEEKA--AREKLEK-EYEEFAKQLKTIL 554

Query: 545 TGKVFDVRTTQKLDSHPCVIVVPE---MAAARHFIRTQAQNLTEENRFALLRPQLEINAK 601
             KV  V  + K+   PC++   +    A     ++ QA   T  + +   R   EIN K
Sbjct: 555 GDKVEKVVVSNKIVGSPCLLTTGQYGWSANMERIMKAQALRDTSMSAYMSSRKTFEINPK 614

Query: 602 HPIVKKLHKLM---STDKELATMVAQQLFSNAMVTAGLVM-DPRNLITHINDLL 651
            PI+ +L K +     +      +A  L+  A++++G  + DP      IN L+
Sbjct: 615 SPIIAELKKKVEENGAEDRSVKDLATILYETALLSSGFTLDDPSAYAQRINRLI 668



 Score =  179 bits (436), Expect = 1e-45
 Identities = 94/214 (43%), Positives = 145/214 (67%), Gaps = 12/214 (5%)

Query: 34  SEKREFQAETRMLLDIVARSLYSDKEVFIRELISNASDALEKFRYLSVSSVPESGNLKEL 93
           +E  +F+AE   L+ ++  ++YS+KE+F+RELISNASDAL+K RY S+S  P +    + 
Sbjct: 4   TETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQSLSD-PHA---LDA 59

Query: 94  DRPLEIRLTTDKQNRTITFQDSGIGMTKEELTQNLGTIARSGSKSFIEEIKKQGAEQASS 153
           ++ L IR+T DK+N+ ++ +D+GIGMTK +L  NLG IA+SG+K F+ E    GA+   S
Sbjct: 60  EKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFM-EAAASGAD--IS 116

Query: 154 IIGQFGVGFYSAFMVADKIEVFTRSSKGDSPGYQWSSDGSGTYEIQ-EADGVEI--GTKI 210
           +IGQFGVGFYSA++VADK++V   S   D   Y W S   G++ +  + DG  +  GT+I
Sbjct: 117 MIGQFGVGFYSAYLVADKVQVV--SKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEI 174

Query: 211 VVYLKTDCREFADDDTVKSIIKKYSNFVSSPIFL 244
            +++K D  ++ ++ T+K  +KK+S F+S PI L
Sbjct: 175 RLFMKEDQLQYLEEKTIKDTVKKHSEFISYPIQL 208


>SPAC1006.03c |||human CCDC131 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 714

 Score = 30.7 bits (66), Expect = 0.67
 Identities = 36/148 (24%), Positives = 64/148 (43%), Gaps = 7/148 (4%)

Query: 121 KEELTQNLGTIARSGSKSFIEEIKKQGAEQASSIIGQFGVGFYSAFMVADKIE---VFTR 177
           K E +  L T +++   ++ +   ++ AE+A S + + GV F     +A+ IE   V T 
Sbjct: 172 KSEKSPFLST-SKNSDANYSKTTNQKEAEKAVSQLFEVGVRFND--FIAEGIEPSVVHTL 228

Query: 178 SSKGDSPGYQWSSDGSGTYEIQEADGVEIGTKIVVYLKTDCREFADDDTVKSIIKKYSNF 237
             K        SS GS T    +A       KI   L +D      D+   +++ +  N 
Sbjct: 229 FLKLGLDSSSASSQGSLTLSADKAARSAKLRKIDSNL-SDTHILPGDNGTPTVLPERKNL 287

Query: 238 VSSPIFLNNDQVNSIKPLWLFEPKEITQ 265
           +S P+   +D ++S KP     P  + +
Sbjct: 288 ISLPLLKQDDWLSSSKPFGSSTPNVVIE 315


>SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma
           catalytic subunit|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1018

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 13/46 (28%), Positives = 24/46 (52%)

Query: 213 YLKTDCREFADDDTVKSIIKKYSNFVSSPIFLNNDQVNSIKPLWLF 258
           Y+++   E ++DD   S    Y N++    +L +  VNS+K +  F
Sbjct: 274 YIRSQSTETSEDDDSSSFDDDYQNYLKQEPWLAHSSVNSLKDVAKF 319


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 1/64 (1%)

Query: 11  KLKEFQHFSTVTEKQSSESLKGKSEKREFQAETRMLLDIVARSLYSDKEVF-IRELISNA 69
           K +E +H    T+K  +E++  K+   E+  +TR  LD +     +++E    +E+++ A
Sbjct: 558 KYREAEHQMIATDKLVAETVDRKNALEEYIYDTRAKLDDIYAPFTNEEESSKFKEMLTKA 617

Query: 70  SDAL 73
            D L
Sbjct: 618 EDWL 621


>SPCC613.11c |meu23|B13958-2|DUF1773 family protein
           2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 254

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 26/110 (23%), Positives = 54/110 (49%), Gaps = 5/110 (4%)

Query: 35  EKREFQAETRMLLDIVARSLYSDKEVFIRELISNASDALEKF-RYLSVSSVPESGNLKEL 93
           EKR  +A  R +L+   R+L +   +   E+ +N  + +++F R +   +  E     ++
Sbjct: 41  EKRRIEARNR-ILESEGRALENKTHLNEEEIRTN--ERVKRFQRRVDEWTALEIERESQV 97

Query: 94  DRPLEIRLTTDKQNRTITFQDSGIGMTKEELTQNLGTI-ARSGSKSFIEE 142
             PL  +   ++QNRT+ F  SG+   +  +    GT+    G+ + ++E
Sbjct: 98  QPPLWYQRDREEQNRTLNFLMSGMADLQRTINTMQGTMNTMQGTMNTMQE 147


>SPBC1604.16c |||RNA-binding protein, G-patch type
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 199

 Score = 28.7 bits (61), Expect = 2.7
 Identities = 16/64 (25%), Positives = 28/64 (43%)

Query: 123 ELTQNLGTIARSGSKSFIEEIKKQGAEQASSIIGQFGVGFYSAFMVADKIEVFTRSSKGD 182
           EL+ + G        SF++   +Q  ++A+ I       FY +    D+    T +SK D
Sbjct: 8   ELSNSYGFYKNGRRVSFVKATSEQSLDEATFIEKGSAQAFYHSLFENDRDNSHTMNSKRD 67

Query: 183 SPGY 186
             G+
Sbjct: 68  EAGF 71


>SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1018

 Score = 27.5 bits (58), Expect = 6.3
 Identities = 20/59 (33%), Positives = 30/59 (50%), Gaps = 4/59 (6%)

Query: 40  QAETRMLLDIVARSLYSDKEVFIRELISNASDALEKFRYLSVSSVPESGNLKELDRPLE 98
           Q   R+L D+  +S  S   VF     S AS ++E F Y S + +P   +L  ++ PLE
Sbjct: 345 QLSQRLLEDVNVKSCGSCAAVFA----SLASISVETFNYCSCNFLPSVLDLPMVNEPLE 399


>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 27.5 bits (58), Expect = 6.3
 Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 1/80 (1%)

Query: 67  SNASDALEKFRYLSVSSVPESGNLKELDRPLEIRLTTDKQNRTITFQDSGIGMTKEELTQ 126
           S  SD    F    +++     +LK  + PL  R  T KQNR + F      +++  ++ 
Sbjct: 39  SRLSDGALNFTGGRIATPLPQPSLKTPESPLSKRNPTIKQNR-VRFDLPDDELSRSNVSS 97

Query: 127 NLGTIARSGSKSFIEEIKKQ 146
              T+  S S S  + +KK+
Sbjct: 98  PEKTLLTSASTSTFDSLKKE 117


>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
           Rad50|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1290

 Score = 27.5 bits (58), Expect = 6.3
 Identities = 26/116 (22%), Positives = 51/116 (43%), Gaps = 9/116 (7%)

Query: 401 YSIFLKEGIVTSQSPLEKEEIAKLLRFESSKLS----NGQRTSLSDYSSRQKDDQKSI-- 454
           Y+  + +    S   L++ ++A   R E  K      N + + ++    +Q   QK +  
Sbjct: 392 YASLVNKNYEISSGKLKERQVAVRARIEGIKAHEMFLNNRVSEINSSLEKQLTTQKELRS 451

Query: 455 -FYLAAPSRELAETSPYYESLKKRDIEVLFCYEMYDELVLLELKEFGGRALVSVEN 509
            F +  P +   +   + + ++K D+ +    + Y+   LLEL +    AL SVEN
Sbjct: 452 RFEILFPVK--LQREDFTKDVEKSDLWIKSLRQEYESKNLLELLDKHQTALSSVEN 505


>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1234

 Score = 27.1 bits (57), Expect = 8.3
 Identities = 14/40 (35%), Positives = 21/40 (52%)

Query: 59  EVFIRELISNASDALEKFRYLSVSSVPESGNLKELDRPLE 98
           ++   EL+S AS+ L +F    V  +PES   K L   +E
Sbjct: 407 QIVYPELLSIASERLLRFEDAVVELIPESATAKYLQEDVE 446


>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1210

 Score = 27.1 bits (57), Expect = 8.3
 Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 2/34 (5%)

Query: 324  SRKILIKSKAENILPKWLRFVKGVVDSEDIPLNL 357
            SR +LIK KA+N++    R  + ++ SE+I L++
Sbjct: 985  SRSVLIKPKADNVI--LFRLKRFIMSSEEINLDI 1016


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 27.1 bits (57), Expect = 8.3
 Identities = 27/102 (26%), Positives = 47/102 (46%), Gaps = 7/102 (6%)

Query: 10  SKLKEFQHFSTVTEK-QSSESLKGKSEKREFQAETRMLLDIVARSLYSDKEVFIRELISN 68
           SK  E  H   +TE  ++ ES+  KS   E++    ++  +++RSL  +K          
Sbjct: 526 SKSTEALHSGNLTEHLKTVESILSKSRHEEYRIANHIVAYLLSRSLKQNKTESDNSFAIL 585

Query: 69  ASDALEKFRYLSVSSVPE-SGN---LKELDRPLEIRLTTDKQ 106
               LE    L++ S+PE  G    +++    L + +T DKQ
Sbjct: 586 TKILLEDL--LNMLSLPEWCGTETIIRQFAMNLVMTVTNDKQ 625


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.316    0.133    0.364 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,852,269
Number of Sequences: 5004
Number of extensions: 122217
Number of successful extensions: 427
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 414
Number of HSP's gapped (non-prelim): 14
length of query: 658
length of database: 2,362,478
effective HSP length: 77
effective length of query: 581
effective length of database: 1,977,170
effective search space: 1148735770
effective search space used: 1148735770
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 57 (27.1 bits)

- SilkBase 1999-2023 -