Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001238-TA|BGIBMGA001238-PA|IPR009080|Aminoacyl-tRNA
synthetase, class 1a, anticodon-binding, IPR002308|Cysteinyl-tRNA
synthetase, class Ia
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 497 e-141
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 46 2e-05
SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 35 0.032
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 31 0.70
SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0... 30 1.2
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe... 29 2.1
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 29 2.8
SPCC1450.13c |||riboflavin synthase |Schizosaccharomyces pombe|c... 28 3.7
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 28 3.7
SPAC22A12.07c |ogm1|oma1|protein O-mannosyltransferase Ogm1|Schi... 28 4.9
SPCC1827.02c |||cholinephosphate cytidylyltransferase |Schizosac... 27 6.5
SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyce... 27 8.6
SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 27 8.6
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 27 8.6
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 497 bits (1226), Expect = e-141
Identities = 286/709 (40%), Positives = 384/709 (54%), Gaps = 41/709 (5%)
Query: 5 SQPAWSPPVRNKERPVLKLYNSLSRQKEEFIPANGNRVNWYSCGPTVYDASHMGHARSYM 64
+Q W P +R L +YN+L+ K F+ +NG+ + WY CGPTVYDASHMGHAR+Y+
Sbjct: 7 AQSHWGVP--KGQRTELYVYNTLTHSKVPFV-SNGSNLTWYCCGPTVYDASHMGHARNYV 63
Query: 65 SFDILRRVMANYFGYDILYVMNITDIDDKIIKRARQKYLYEKYLKEPRNLNDTVDDAVNV 124
+ DILRR++ +YFGY+I +V N+TDIDDKII RARQ+YL+E+Y K+ + N + +A
Sbjct: 64 TTDILRRILQSYFGYNITFVQNVTDIDDKIILRARQQYLFEEYKKQ-QGTNKSHSEAREK 122
Query: 125 IN--YYEEAVKNAEDPDKKK------LMQKTLDEIAAS-------VKALKVAIESKDNER 169
+ ++ A KN +P L + +A + V ALK A+++
Sbjct: 123 VTEAWFAYAKKNLPEPPSSMSEWPQWLASHDIPTLAINNPKLPMHVDALKSALDALQVSE 182
Query: 170 IKN--SRCDLLTSAKSPISDWLDYKYGETVTDNAIFTTLPRYWENEFHKDMKALNVLPPD 227
S + + LD + G TVTD AIF L YWE++F+KDM LNVLPP
Sbjct: 183 ASEIISLDGFWPKVQDVLVPLLDAELGSTVTDPAIFRKLAAYWEDDFNKDMANLNVLPPT 242
Query: 228 VLTRVSEYVPKIIAYIQKIISNGFAYE-SNGSVYFNVSEFDGNERHHYARLVPEAYGDTK 286
+TRVSEYVP+I+ ++Q+II G+AY ++GSVYF+ F+ H YA+L P G+ +
Sbjct: 243 AVTRVSEYVPEIVDFVQRIIDRGYAYPVTDGSVYFDTEAFEKGG-HFYAKLEPWNKGNRE 301
Query: 287 SLQEGEGDLTDDAAEKRSPNDFALWKRSKAGEPSWDSPWGAGRPGWHIECSAMASDVCQN 346
+ EGEG L +KR P DFALWK SK GEPSWDSPW GRPGWHIECS MAS + +
Sbjct: 302 LIAEGEGSLAALTGKKR-PGDFALWKASKPGEPSWDSPWSKGRPGWHIECSVMASALLGS 360
Query: 347 KLDIHTGGVDLKFPHHDNELAQSEAHFDEPGWVNYFLHTGHLMIAGCKMSKSLKNFITIS 406
+DIH+GG+DL FPHHDNELAQSEA+FD P WVNYF H GHL I G KMSKSLKNFITI
Sbjct: 361 NIDIHSGGIDLAFPHHDNELAQSEAYFDCPQWVNYFFHAGHLHIEGQKMSKSLKNFITIK 420
Query: 407 DALKRHTARQLRFGFLLHGWKETLDYSENTMEMAIQTEKQFNEFFLTVKDALRSGYDEGC 466
+ LK+ T RQLR FLL W LD+ E + + E+ FF TV+ +
Sbjct: 421 EILKKFTPRQLRLAFLLQQWNTQLDFKETLLAYVLNIEQALENFFRTVRALMNETEGVSA 480
Query: 467 GGGWEPE-----EQRLFAKLSMAKEQVHAALCDNIDTRGAIDALRELVCACHVYXXXXXX 521
GG PE E L K ++ H ALCD+ +T + + LV ++Y
Sbjct: 481 NGGHVPEKFDKLEIELLEKFQQTQQNTHLALCDSFNTPLVMQHIDNLVTQANIYIREVGQ 540
Query: 522 X-XXXXXXXXXXYVTDVLHVFGVVE-GPRGGIGFPVGDADGVSLEET-VMPYLEALSAFX 578
++T +L +FG+ E G +G+ + G S E T MPY+ A+S+F
Sbjct: 541 QPCSRLLGQIASWITSMLQIFGLDENGHPNAVGW--SSSKGSSSENTDAMPYIRAVSSFR 598
Query: 579 XXXXXX-XXXXXXXXXLALCDALRDRVLPDLGVRLEDKPD-RTVVKLVSXXXXXXXXXXX 636
L CD RD + LGV D+P +VKLV
Sbjct: 599 DRVRELCIAKASSQEILKACDIFRDYDMAALGVSFNDRPQGSALVKLVDAEELIAAREQK 658
Query: 637 XXXXXXXXXXXXXXXXXXRLKEEQK----KISPAEMFRKETDKYSKFDE 681
K+ ++ K SP+EMF K +Y FDE
Sbjct: 659 LEEERAKQAKKAQAKAEQEKKQVERVMKGKTSPSEMF-KMFKEYLSFDE 706
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 46.0 bits (104), Expect = 2e-05
Identities = 36/117 (30%), Positives = 54/117 (46%), Gaps = 4/117 (3%)
Query: 349 DIHTGGVDLKFPHHDNELAQSEAHFDEPGWVNYFLHTGHLMIAGCKMSKSLKNFITISDA 408
DI G DL H L A FDE W GHL++ G KMSKS NF+T+ +A
Sbjct: 692 DIRASGKDLVPNHLTFCLYTHTAIFDEKYWPKGIRANGHLLMNGEKMSKSTGNFMTLHEA 751
Query: 409 LKRHTARQLRFGFLLHG-WKETLDYSE---NTMEMAIQTEKQFNEFFLTVKDALRSG 461
K+ A R G + ++ E N+ + + T++ + + + D LR+G
Sbjct: 752 TKKFGADATRLALADAGDTVDDANFEEALANSAILRLYTQEAWCKEMMENLDNLRTG 808
>SPBC30B4.02c |||R3H and G-patch domain, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 35.1 bits (77), Expect = 0.032
Identities = 22/71 (30%), Positives = 37/71 (52%), Gaps = 6/71 (8%)
Query: 254 ESNGSVYFNVS-----EFD-GNERHHYARLVPEAYGDTKSLQEGEGDLTDDAAEKRSPND 307
E+ G V F++ EFD G++ + YA+L E G+T SL E E D+ ++ ++
Sbjct: 297 ETEGKVNFSLEDSESLEFDEGSDDYDYAKLTTELVGNTDSLAEDEDDILEEDEDEDEEEL 356
Query: 308 FALWKRSKAGE 318
A++ S E
Sbjct: 357 LAIFDASDDSE 367
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 30.7 bits (66), Expect = 0.70
Identities = 16/64 (25%), Positives = 34/64 (53%)
Query: 111 PRNLNDTVDDAVNVINYYEEAVKNAEDPDKKKLMQKTLDEIAASVKALKVAIESKDNERI 170
P+N D + D ++ ++ EE +++E KKK K+ D+ + ++ + KD ++
Sbjct: 122 PKNAFDALADDMDDLSLDEEESESSEKSKKKKKKSKSKDDGSEALDDGDIESSEKDKKKK 181
Query: 171 KNSR 174
K S+
Sbjct: 182 KKSK 185
>SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 292
Score = 29.9 bits (64), Expect = 1.2
Identities = 23/77 (29%), Positives = 34/77 (44%), Gaps = 9/77 (11%)
Query: 270 ERHHYARLVPEAYGDTKSLQEG----EGDLTDDAAEKRSPNDFALWKRSKAGEPSWDSPW 325
ER A+ A + ++E E ++TD AA PN A + GE +W+
Sbjct: 213 EREEEAKKAAAAAAEEAQVEEAVAAAEFEITDSAAGSVDPNVLAAATAGQVGENTWE--- 269
Query: 326 GAGRPGWHIECSAMASD 342
GAG W+ +A SD
Sbjct: 270 GAG--DWNTTGAAQTSD 284
>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 504
Score = 29.1 bits (62), Expect = 2.1
Identities = 16/49 (32%), Positives = 24/49 (48%)
Query: 128 YEEAVKNAEDPDKKKLMQKTLDEIAASVKALKVAIESKDNERIKNSRCD 176
Y + K D +MQ+ L + K K+ IE K++E IKN + D
Sbjct: 454 YHKGYKYNPDYKDGLVMQEYLPDSIKGTKFYKLPIELKEDEEIKNLKTD 502
>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 716
Score = 28.7 bits (61), Expect = 2.8
Identities = 15/40 (37%), Positives = 22/40 (55%)
Query: 220 ALNVLPPDVLTRVSEYVPKIIAYIQKIISNGFAYESNGSV 259
AL + PDV+T S+Y+ I Y +I +G AY + V
Sbjct: 266 ALLGIKPDVVTYTSDYLDTIHQYCVDMIKSGQAYADDTDV 305
>SPCC1450.13c |||riboflavin synthase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 208
Score = 28.3 bits (60), Expect = 3.7
Identities = 17/61 (27%), Positives = 25/61 (40%)
Query: 85 MNITDIDDKIIKRARQKYLYEKYLKEPRNLNDTVDDAVNVINYYEEAVKNAEDPDKKKLM 144
+ IT +DD Y K + +N+ D V+ V+ I Y E + A D K
Sbjct: 147 LTITHVDDSTFSIMMISYTQSKVIMAKKNVGDLVNVEVDQIGKYTEKLVEAHIADWIKKT 206
Query: 145 Q 145
Q
Sbjct: 207 Q 207
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 28.3 bits (60), Expect = 3.7
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
Query: 337 SAMASDVCQNKLDIHTGGVDLKFPHHDN-ELAQSEAHFDEPGW 378
S+ D+ Q +D+ GGVD F N + Q E F GW
Sbjct: 245 SSKVKDLVQYVIDVTDGGVDYAFDCTGNVTVMQQELQFCHKGW 287
>SPAC22A12.07c |ogm1|oma1|protein O-mannosyltransferase
Ogm1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 893
Score = 27.9 bits (59), Expect = 4.9
Identities = 20/88 (22%), Positives = 40/88 (45%), Gaps = 6/88 (6%)
Query: 173 SRCDLLTSAKSPISDWLDYKYGETVTDNAIFTTLPRYWENEFH----KDMKALNVLPPDV 228
+ C+L++S + DW DY+ T N + + + E+ +H D + + P
Sbjct: 460 ANCELMSSHRR-FPDWGDYQREVTCCRNCVERSTTWFIESNYHDGLPSDSRKITYRKPGF 518
Query: 229 LTRVSEYVPKIIAYIQKIISNGFAYESN 256
L E+ K++ + + +G YES+
Sbjct: 519 LESFVEH-NKLMWLKDRKMGDGHVYESS 545
>SPCC1827.02c |||cholinephosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 354
Score = 27.5 bits (58), Expect = 6.5
Identities = 25/90 (27%), Positives = 42/90 (46%), Gaps = 12/90 (13%)
Query: 103 LYEKYLKEPRNLNDTVDDAVNVINYY-----EEAVKNAEDPDKKKLMQKTLDEIAASVKA 157
L + Y EP D V+ N+ E+ K+ ++ DK++ K+L+EI+ SV
Sbjct: 5 LAKMYEVEPSEKEDNVERQTKKYNFEIDEPEEQEKKDEKEDDKEESPSKSLEEISQSVSP 64
Query: 158 LKVAIESKDNERIKNSRCDLLTSAKSPISD 187
++ E + R K +L T PI+D
Sbjct: 65 VE---EEPRDVRFK----ELSTPFSYPIND 87
>SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 855
Score = 27.1 bits (57), Expect = 8.6
Identities = 12/29 (41%), Positives = 19/29 (65%), Gaps = 2/29 (6%)
Query: 355 VDLKFPHHDNELAQSEAHFDEPGWVNYFL 383
++LKF H N +Q+ A+FD+ G NY +
Sbjct: 482 LELKFKGHANAQSQNRAYFDDDG--NYVI 508
>SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 27.1 bits (57), Expect = 8.6
Identities = 12/52 (23%), Positives = 29/52 (55%)
Query: 123 NVINYYEEAVKNAEDPDKKKLMQKTLDEIAASVKALKVAIESKDNERIKNSR 174
+ ++Y+ A+K ++ K++++ + E + LKV ++ K+N+ SR
Sbjct: 35 SALDYFMMALKYEKNEKSKEIIRSKVIEYLDRAEKLKVYLQEKNNQISSKSR 86
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 27.1 bits (57), Expect = 8.6
Identities = 11/59 (18%), Positives = 31/59 (52%)
Query: 404 TISDALKRHTARQLRFGFLLHGWKETLDYSENTMEMAIQTEKQFNEFFLTVKDALRSGY 462
++SD +HT+ + ++ + L ++ S+ EM + + +++E+ L ++L +
Sbjct: 920 SLSDLQSKHTSLESQYNYSLRNIEQLQAASKLAEEMVERVKTEYDEYRLQTSESLEKNH 978
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.135 0.407
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,986,266
Number of Sequences: 5004
Number of extensions: 123043
Number of successful extensions: 349
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 334
Number of HSP's gapped (non-prelim): 17
length of query: 685
length of database: 2,362,478
effective HSP length: 78
effective length of query: 607
effective length of database: 1,972,166
effective search space: 1197104762
effective search space used: 1197104762
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 57 (27.1 bits)
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