Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001238-TA|BGIBMGA001238-PA|IPR009080|Aminoacyl-tRNA
synthetase, class 1a, anticodon-binding, IPR002308|Cysteinyl-tRNA
synthetase, class Ia
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31170.1 68415.m03805 tRNA synthetase class I (C) family prot... 193 3e-49
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 183 4e-46
At3g56300.1 68416.m06258 tRNA synthetase class I (C) family prot... 161 2e-39
At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)... 38 0.029
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 35 0.16
At2g32820.1 68415.m04017 hypothetical protein and genscan 33 0.83
At1g53840.1 68414.m06128 pectinesterase family protein contains ... 32 1.5
At3g27290.1 68416.m03411 F-box family protein-related contains w... 31 3.4
At1g20080.1 68414.m02513 C2 domain-containing protein contains I... 30 4.4
At5g20360.1 68418.m02422 octicosapeptide/Phox/Bem1p (PB1) domain... 29 7.7
At5g13460.1 68418.m01549 calmodulin-binding family protein low s... 29 7.7
At2g47310.1 68415.m05906 flowering time control protein-related ... 29 7.7
>At2g31170.1 68415.m03805 tRNA synthetase class I (C) family protein
similar to cysteine-tRNA ligase [Escherichia coli]
GI:41203; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 563
Score = 193 bits (471), Expect = 3e-49
Identities = 99/236 (41%), Positives = 143/236 (60%), Gaps = 11/236 (4%)
Query: 205 TLPRYWENEFHKDMKALNVLPPDVLTRVSEYVPKIIAYIQKIISNGFAYESNGSVYFNVS 264
+L R + EF++DM+ L L P V RVS+++P+II I++I+ NG+AY+ +G +YF+V
Sbjct: 150 SLSRRFCEEFNRDMEQLQCLDPSVQPRVSDHIPQIIDLIKQILDNGYAYKVDGDIYFSVD 209
Query: 265 EFDGNERHHYARLVPEAYGDTKSLQEGEGDLTDDAAEKRSPNDFALWKRSKAGEPSWDSP 324
+F Y +L D ++ GE D K+ P DFALWK +K GEP W+SP
Sbjct: 210 KFPT-----YGKLSGRKLEDNRA---GERVAVD--TRKKHPADFALWKAAKEGEPFWESP 259
Query: 325 WGAGRPGWHIECSAMASDVCQNKLDIHTGGVDLKFPHHDNELAQSEAHFDEPGWVNYFLH 384
WG GRPGWHIECSAM++ DIH GG+DL FPHH+NE+AQS A D ++Y++H
Sbjct: 260 WGRGRPGWHIECSAMSAAYLGYSFDIHGGGMDLVFPHHENEIAQSCAACDSSN-ISYWIH 318
Query: 385 TGHLMIAGCKMSKSLKNFITISDALKRHTARQLRFGFLLHGWKETLDYSENTMEMA 440
G + + KMSKSL NF TI + + LR + ++ ++YS+ +E A
Sbjct: 319 NGFVTVDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLMGTHYRSPINYSDFLLESA 374
Score = 91.5 bits (217), Expect = 2e-18
Identities = 41/80 (51%), Positives = 57/80 (71%), Gaps = 1/80 (1%)
Query: 21 LKLYNSLSRQKEEFIPANGNRVNWYSCGPTVYDASHMGHARSYMSFDILRRVMANYFGYD 80
L L+N++SR+KE F P +V Y CG T YD SH+GHAR Y++FD+L R + + GY+
Sbjct: 65 LWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLLRYL-KHLGYE 123
Query: 81 ILYVMNITDIDDKIIKRARQ 100
+ YV N TD+DDKII RA++
Sbjct: 124 VSYVRNFTDVDDKIIARAKE 143
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 183 bits (445), Expect = 4e-46
Identities = 107/289 (37%), Positives = 156/289 (53%), Gaps = 14/289 (4%)
Query: 212 NEFHKDMKALNVLPPDVLTRVSEYVPKIIAYIQKIISNGFAYESNGSVYFNVSEFDGNER 271
+E+ DM AL LPP RVSE++ II I+KII Y G V+F+V ++
Sbjct: 100 DEYLVDMGALQCLPPTHQPRVSEHMDNIIKMIEKIIEKDCGYVVEGDVFFSV-----DKS 154
Query: 272 HHYARLVPEAYGDTKSLQEGEGDLTDDAAEKRSPNDFALWKRSKAGEPSWDSPWGAGRPG 331
+Y +L + T++ GE D + KR+P DFALWK +K EPSW+SPWG GRPG
Sbjct: 155 PNYGKLSGQLLEHTRA---GERVAVD--SRKRNPADFALWKAAKPDEPSWESPWGPGRPG 209
Query: 332 WHIECSAMASDVCQNKLDIHTGGVDLKFPHHDNELAQSEAHFDEPGWVNYFLHTGHLMIA 391
WHIECSAM+ K DIH GG DLKFPHH+NE+AQ+ A ++ G VNY+LH GH+ I
Sbjct: 210 WHIECSAMSVHYLSPKFDIHGGGADLKFPHHENEIAQTCAACEDSG-VNYWLHNGHVTIN 268
Query: 392 GCKMSKSLKNFITISDALKRHTARQLRFGFLLHGWKETLDYSENTMEMAIQTEKQFNEFF 451
KM+KS NF TI + + LR + ++ L ++ + +E + + +
Sbjct: 269 NEKMAKSKHNFKTIREITASYHPLALRHFLMSAQYRSPLSFTASQLESSSEALYYVYQTL 328
Query: 452 LTVKDALRSGYDEGCGGGWEPEEQRLFAK--LSMAKEQVHAALCDNIDT 498
+ + L S Y + EQ K + K + + + D+++T
Sbjct: 329 QDLDEGL-SPYQDALSEDGGKSEQTAEGKDIIKKLKTEFESKMLDDLNT 376
Score = 92.3 bits (219), Expect = 1e-18
Identities = 41/84 (48%), Positives = 58/84 (69%), Gaps = 1/84 (1%)
Query: 17 ERPVLKLYNSLSRQKEEFIPANGNRVNWYSCGPTVYDASHMGHARSYMSFDILRRVMANY 76
E+ LKLYN++++QKE IP ++ Y CG T YD SH+GHAR+ +SFD+L R + +
Sbjct: 4 EKMELKLYNTMTQQKEVLIPITPGKIGLYVCGITAYDFSHIGHARAAVSFDVLYRYL-KH 62
Query: 77 FGYDILYVMNITDIDDKIIKRARQ 100
YD+ +V N TD+DDKII RA +
Sbjct: 63 LDYDVTFVRNFTDVDDKIIDRANK 86
>At3g56300.1 68416.m06258 tRNA synthetase class I (C) family protein
similar to cysteinyl-tRNA synthetase [Methanococcus
maripaludis] GI:6599476; contains Pfam profile PF01406:
tRNA synthetases class I (C)
Length = 489
Score = 161 bits (390), Expect = 2e-39
Identities = 95/226 (42%), Positives = 126/226 (55%), Gaps = 23/226 (10%)
Query: 213 EFHKDMKALNVLPPDVLTRVSEYVPKIIAYIQKIISNGFAYESNGSVYFNVSEFDGNERH 272
E+ DM AL L P RVS+++ +II I+KII NG Y G V+F+V +
Sbjct: 100 EYLLDMAALQCLLPTHQPRVSDHMEQIIKMIEKIIENGCGYAVGGDVFFSVDKSPS---- 155
Query: 273 HYARLVPEAYGDTKSLQEGEGDLTDDAAEKRSPNDFALWKRSKAGEPSWDSPWGAGRPGW 332
Y +L + T Q G+ D + KR+P DFAL K +K+GEPSW+SPWG GRPGW
Sbjct: 156 -YGQLSGQRLDHT---QAGKRVAVD--SRKRNPADFALRKAAKSGEPSWESPWGHGRPGW 209
Query: 333 HIECSAMASDVCQNKLDIHTGGVDLKFPHHDNELAQSEAHFDEPGWVNYFLHTGHLMIAG 392
HIE DIH GG DLKFPHH+NE+AQ+ A ++ G VNY+LH GH+
Sbjct: 210 HIE------------FDIHGGGADLKFPHHENEIAQTCAACEDSG-VNYWLHNGHVTNNN 256
Query: 393 CKMSKSLKNFITISDALKRHTARQLRFGFLLHGWKETLDYSENTME 438
KM KSL NF TI + LR + ++ L+YS + +E
Sbjct: 257 VKMGKSLNNFFTIRQIAANYHPLALRHFLMSAQYRSPLNYSVSQLE 302
Score = 85.4 bits (202), Expect = 1e-16
Identities = 37/77 (48%), Positives = 53/77 (68%), Gaps = 1/77 (1%)
Query: 17 ERPVLKLYNSLSRQKEEFIPANGNRVNWYSCGPTVYDASHMGHARSYMSFDILRRVMANY 76
E+P L LYN++++ KE + P N ++ Y CG T YD SH+GHAR+ +SFD+L R + +
Sbjct: 7 EKPDLTLYNTMTQLKEVYKPMNPGKIGIYVCGITAYDYSHIGHARAAVSFDLLYRYL-RH 65
Query: 77 FGYDILYVMNITDIDDK 93
GY + YV N TD+DDK
Sbjct: 66 LGYQVTYVRNFTDVDDK 82
>At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)
family protein similar to cytosolic leucyl-tRNA
synthetase [Candida albicans] GI:9858190; contains Pfam
profile PF00133: tRNA synthetases class I (I, L, M and
V)
Length = 1084
Score = 37.5 bits (83), Expect = 0.029
Identities = 33/118 (27%), Positives = 50/118 (42%), Gaps = 4/118 (3%)
Query: 348 LDIHTGGVDLKFPHHDNELAQSEAHFDEPGWVNYFLHTGHLMIAGCKMSKSLKNFITISD 407
LD+ G DL H + A W GH+M+ KMSKS NF T+
Sbjct: 669 LDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMSKSTGNFRTLRQ 728
Query: 408 ALKRHTARQLRFGFLLHG-WKETLDYSENTMEMAI---QTEKQFNEFFLTVKDALRSG 461
+++ +A RF G + +++ T AI E + E L V+ +LR+G
Sbjct: 729 SIEEFSATGTRFCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVLDVESSLRTG 786
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 35.1 bits (77), Expect = 0.16
Identities = 24/86 (27%), Positives = 45/86 (52%), Gaps = 3/86 (3%)
Query: 92 DKIIKRARQKYLYEKYLKEPRNLNDTVDDAVNVINYYEEA--VKNAEDPDKKKLMQKTLD 149
++ IK AR+K E+ +KE R + + E+ +K ++ ++ + K +
Sbjct: 737 ERRIKEAREKEENERRIKEAREKAELEQRLKATLEQEEKERQIKERQEREENERRAKEVL 796
Query: 150 EIAASVKALKVAIESKDNE-RIKNSR 174
E A + + LK A+E K+NE R+K +R
Sbjct: 797 EQAENERKLKEALEQKENERRLKETR 822
>At2g32820.1 68415.m04017 hypothetical protein and genscan
Length = 333
Score = 32.7 bits (71), Expect = 0.83
Identities = 21/72 (29%), Positives = 31/72 (43%), Gaps = 3/72 (4%)
Query: 362 HDNELAQSEAHFDEPGWVNYFLHTGHLMIAGCKMSK--SLKNFITISDALKRHTARQLRF 419
H+N ++EAH W+ F +G + C LK + LK+ QL
Sbjct: 68 HENPKIRTEAHVLFTSWMKTFYSSGQNSSSTCNKPNPLKLKKVVKACSELKKKNEEQLSH 127
Query: 420 GF-LLHGWKETL 430
GF +L KET+
Sbjct: 128 GFAVLKAKKETV 139
>At1g53840.1 68414.m06128 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 586
Score = 31.9 bits (69), Expect = 1.5
Identities = 30/137 (21%), Positives = 59/137 (43%), Gaps = 8/137 (5%)
Query: 113 NLNDTVDDAVNVINYYEEAVKNAEDPDKKKLMQKTLDEIAASVKALKVAIESKDNERIKN 172
+L +D+ ++ + E+ K ED K ++ D I ++ L + + D+E K
Sbjct: 108 SLKVIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKK 167
Query: 173 SRCDLLTSAKSPISDWLDYKYGETVTDN-AIFTTLPRYWENEFHKDMKALNVLPPDVLTR 231
+ L +S + WL TVTD+ F +L +N+ + ++R
Sbjct: 168 T---LSSSKIEDLKTWL----SATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSR 220
Query: 232 VSEYVPKIIAYIQKIIS 248
+E+ +A + KI+S
Sbjct: 221 STEFTSNSLAIVSKILS 237
>At3g27290.1 68416.m03411 F-box family protein-related contains weak
similarity to PPA [Mus musculus] GP|18568225|gb|AAL75967
Length = 382
Score = 30.7 bits (66), Expect = 3.4
Identities = 18/71 (25%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Query: 144 MQKTLDEIAASVKALKVAIESKDNERIKNSRCDLLTSAKSPISDWLDYKYGET-VTDNAI 202
++ TL+ I+ ++ + S+D+ ++ C + S + ISDW E V D +
Sbjct: 41 IRSTLNSISDWFHENQIGLVSEDHTLPEDPFCMRVRSTLNTISDWFHENQKEIGVVDQML 100
Query: 203 FTTLPRYWENE 213
F TL ++ N+
Sbjct: 101 FETLSWFYNND 111
>At1g20080.1 68414.m02513 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 535
Score = 30.3 bits (65), Expect = 4.4
Identities = 25/104 (24%), Positives = 45/104 (43%), Gaps = 3/104 (2%)
Query: 124 VINYYEEAV-KNAEDPDKKKLMQKTLDEIAASVKALKVAIESKDNERIKNSRCDLLTSAK 182
VI YY ++ +DP+ K L++ + IA + + +++ D +RI + L
Sbjct: 22 VIGYYLFIYFQSTDDPEIKPLVELDSETIATMFPEIPMWVKNPDFDRI--DWLNKLIGHM 79
Query: 183 SPISDWLDYKYGETVTDNAIFTTLPRYWENEFHKDMKALNVLPP 226
P D K +++ I +P Y + +M L LPP
Sbjct: 80 WPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLTLGSLPP 123
>At5g20360.1 68418.m02422 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein contains Pfam profiles PF00564:
PB1 domain, PF00515: TPR Domain
Length = 809
Score = 29.5 bits (63), Expect = 7.7
Identities = 28/115 (24%), Positives = 46/115 (40%), Gaps = 5/115 (4%)
Query: 116 DTVDDAVNVINYYEEAVKNAEDPDKKKLMQKTLDEIAASVKALK---VAIESKDNERIKN 172
+T+DD V+ + V E D KTLD+ + V+ L +E+ D+ K
Sbjct: 47 ETLDDCVSKAETLADCVSKVETLDDCVSKVKTLDDCVSKVENLDDCVPKVETLDDCVPKV 106
Query: 173 SRCDLLTSAKSPISDWLDYKYGETVTDNAIFTTLPRYWENEFHKDMKALNVLPPD 227
D S + D + G N +F R ++ K +A+ +LP D
Sbjct: 107 ETLDDCVSEVETLDDCVSKAQGLKEEGNKLFQ--KRDYDGAMFKYGEAIKILPKD 159
>At5g13460.1 68418.m01549 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 443
Score = 29.5 bits (63), Expect = 7.7
Identities = 23/103 (22%), Positives = 41/103 (39%), Gaps = 5/103 (4%)
Query: 87 ITDIDDKIIKRARQKYLYEKYLKEPRNLNDTVDDAVNVINYYEEAVKNAEDPDKKKLMQK 146
+ D+D + + + L EK LK PRN + ++N + V ED +
Sbjct: 272 LEDLDFSSKTKPKDETLNEKQLKTPRNSSPR-----RLVNNHRRQVSIGEDEQSPAAVTI 326
Query: 147 TLDEIAASVKALKVAIESKDNERIKNSRCDLLTSAKSPISDWL 189
T + ++ K S + RI+ D + + SP + L
Sbjct: 327 TTPTYMVATESAKAKSRSLSSPRIRPRSFDTQSESYSPYKNKL 369
>At2g47310.1 68415.m05906 flowering time control protein-related /
FCA gamma-related
Length = 512
Score = 29.5 bits (63), Expect = 7.7
Identities = 14/43 (32%), Positives = 24/43 (55%)
Query: 103 LYEKYLKEPRNLNDTVDDAVNVINYYEEAVKNAEDPDKKKLMQ 145
+YE++LKE L D + + N +EA++N+E + L Q
Sbjct: 437 MYERWLKEQTRLQDEKIKSPPLNNESQEAIENSEQVESDVLQQ 479
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.135 0.407
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,373,731
Number of Sequences: 28952
Number of extensions: 628397
Number of successful extensions: 1697
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1677
Number of HSP's gapped (non-prelim): 18
length of query: 685
length of database: 12,070,560
effective HSP length: 86
effective length of query: 599
effective length of database: 9,580,688
effective search space: 5738832112
effective search space used: 5738832112
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)
- SilkBase 1999-2023 -