Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001235-TA|BGIBMGA001235-PA|IPR001474|GTP cyclohydrolase
I
         (232 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g07270.2 68416.m00865 GTP cyclohydrolase I identical to GTP c...   122   2e-28
At3g07270.1 68416.m00866 GTP cyclohydrolase I identical to GTP c...   122   2e-28
At5g44380.1 68418.m05434 FAD-binding domain-containing protein s...    30   1.5  
At4g34900.1 68417.m04949 xanthine dehydrogenase, putative simila...    29   3.5  
At4g34890.1 68417.m04948 xanthine dehydrogenase, putative simila...    29   3.5  
At3g22990.1 68416.m02899 expressed protein                             29   3.5  
At1g69220.1 68414.m07925 serine/threonine protein kinase, putati...    28   4.7  
At3g60290.1 68416.m06739 oxidoreductase, 2OG-Fe(II) oxygenase fa...    28   6.2  
At3g01310.1 68416.m00042 expressed protein similar to unknown pr...    28   6.2  
At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase fa...    27   8.1  
At1g19690.1 68414.m02455 expressed protein similar to (SP:P76370...    27   8.1  

>At3g07270.2 68416.m00865 GTP cyclohydrolase I identical to GTP
           cyclohydrolase I GI:19909132 from [Arabidopsis
           thaliana]; contains Pfam profile: PF01227 GTP
           cyclohydrolase I
          Length = 466

 Score =  122 bits (294), Expect = 2e-28
 Identities = 76/203 (37%), Positives = 118/203 (58%), Gaps = 24/203 (11%)

Query: 35  DLELDHRPPTREALLPDMANSYRLLLTGLGEDPERAGLLKTPERAAKAMLFFTKGYDQSL 94
           +L  +H+P T       + ++ +LLL GL ED  R G+ KTP R AKA+   T+GY Q +
Sbjct: 23  ELAFEHQPETLA-----IQDAVKLLLQGLHEDVNREGIKKTPFRVAKALREGTRGYKQKV 77

Query: 95  EEVLNNAIFDE-DTDE----------MVVVKDIEMFSMCEHHLVPFYGKVSIGYLPQG-K 142
           ++ + +A+F E   DE          +VVV+D++ +S CE  L+PF+ K  IGY+P G +
Sbjct: 78  KDYVQSALFPEAGLDEGVGQAGGVGGLVVVRDLDHYSYCESCLLPFHVKCHIGYVPSGQR 137

Query: 143 ILGLSKLARIVEIFSRRLQVQERLTKQIAIAVTQAVRPAGVAVVIEGVHMCMVMRGVQKI 202
           +LGLSK +R+ ++F++RLQ  +RL   I  A+   V+PAGVAVV+E  H+      +  +
Sbjct: 138 VLGLSKFSRVTDVFAKRLQDPQRLADDICSALQHWVKPAGVAVVLECSHIHFPSLDLDSL 197

Query: 203 N-------SKTVTSTMLGVFRDD 218
           N        K + S+  GVF D+
Sbjct: 198 NLSSHRGFVKLLVSSGSGVFEDE 220



 Score =  119 bits (286), Expect = 2e-27
 Identities = 73/188 (38%), Positives = 103/188 (54%), Gaps = 7/188 (3%)

Query: 50  PDMANSYRLLLTGLGEDPERAGLLKTPERAAKAMLFFTKGYDQSLEEVLNNAIFDEDTDE 109
           P+M ++   +L  LGEDP R  L+ TP R  K ML F +   +      N A  + +  E
Sbjct: 268 PEMVSAVVSILKSLGEDPLRKELIATPTRFLKWMLNFQRTNLEMKLNSFNPAKVNGEVKE 327

Query: 110 MVVVKDIEM--FSMCEHHLVPFYGKVSIGYL-PQGKI---LGLSKLARIVEIFSRRLQVQ 163
             +  ++ M  +SMCEHHL+PFYG V IGY   +G     +G S +  IV  +  +LQVQ
Sbjct: 328 KRLHCELNMPFWSMCEHHLLPFYGVVHIGYFCAEGSNPNPVGSSLMKAIVHFYGFKLQVQ 387

Query: 164 ERLTKQIAIAVTQAVRPAGVAVVIEGVHMCMVMRGVQKINSKTVTSTMLGVFRDDPKTRE 223
           ER+T+QIA  ++  V    V VV E  H CM+ RG++K  S T T  +LG F  D   R 
Sbjct: 388 ERMTRQIAETLSPLVG-GDVIVVAEAGHTCMISRGIEKFGSSTATIAVLGRFSSDNSARA 446

Query: 224 EFLNLVHS 231
            FL+ +H+
Sbjct: 447 MFLDKIHT 454


>At3g07270.1 68416.m00866 GTP cyclohydrolase I identical to GTP
           cyclohydrolase I GI:19909132 from [Arabidopsis
           thaliana]; contains Pfam profile: PF01227 GTP
           cyclohydrolase I
          Length = 466

 Score =  122 bits (294), Expect = 2e-28
 Identities = 76/203 (37%), Positives = 118/203 (58%), Gaps = 24/203 (11%)

Query: 35  DLELDHRPPTREALLPDMANSYRLLLTGLGEDPERAGLLKTPERAAKAMLFFTKGYDQSL 94
           +L  +H+P T       + ++ +LLL GL ED  R G+ KTP R AKA+   T+GY Q +
Sbjct: 23  ELAFEHQPETLA-----IQDAVKLLLQGLHEDVNREGIKKTPFRVAKALREGTRGYKQKV 77

Query: 95  EEVLNNAIFDE-DTDE----------MVVVKDIEMFSMCEHHLVPFYGKVSIGYLPQG-K 142
           ++ + +A+F E   DE          +VVV+D++ +S CE  L+PF+ K  IGY+P G +
Sbjct: 78  KDYVQSALFPEAGLDEGVGQAGGVGGLVVVRDLDHYSYCESCLLPFHVKCHIGYVPSGQR 137

Query: 143 ILGLSKLARIVEIFSRRLQVQERLTKQIAIAVTQAVRPAGVAVVIEGVHMCMVMRGVQKI 202
           +LGLSK +R+ ++F++RLQ  +RL   I  A+   V+PAGVAVV+E  H+      +  +
Sbjct: 138 VLGLSKFSRVTDVFAKRLQDPQRLADDICSALQHWVKPAGVAVVLECSHIHFPSLDLDSL 197

Query: 203 N-------SKTVTSTMLGVFRDD 218
           N        K + S+  GVF D+
Sbjct: 198 NLSSHRGFVKLLVSSGSGVFEDE 220



 Score =  119 bits (286), Expect = 2e-27
 Identities = 73/188 (38%), Positives = 103/188 (54%), Gaps = 7/188 (3%)

Query: 50  PDMANSYRLLLTGLGEDPERAGLLKTPERAAKAMLFFTKGYDQSLEEVLNNAIFDEDTDE 109
           P+M ++   +L  LGEDP R  L+ TP R  K ML F +   +      N A  + +  E
Sbjct: 268 PEMVSAVVSILKSLGEDPLRKELIATPTRFLKWMLNFQRTNLEMKLNSFNPAKVNGEVKE 327

Query: 110 MVVVKDIEM--FSMCEHHLVPFYGKVSIGYL-PQGKI---LGLSKLARIVEIFSRRLQVQ 163
             +  ++ M  +SMCEHHL+PFYG V IGY   +G     +G S +  IV  +  +LQVQ
Sbjct: 328 KRLHCELNMPFWSMCEHHLLPFYGVVHIGYFCAEGSNPNPVGSSLMKAIVHFYGFKLQVQ 387

Query: 164 ERLTKQIAIAVTQAVRPAGVAVVIEGVHMCMVMRGVQKINSKTVTSTMLGVFRDDPKTRE 223
           ER+T+QIA  ++  V    V VV E  H CM+ RG++K  S T T  +LG F  D   R 
Sbjct: 388 ERMTRQIAETLSPLVG-GDVIVVAEAGHTCMISRGIEKFGSSTATIAVLGRFSSDNSARA 446

Query: 224 EFLNLVHS 231
            FL+ +H+
Sbjct: 447 MFLDKIHT 454


>At5g44380.1 68418.m05434 FAD-binding domain-containing protein
           similar to SP|P30986 reticuline oxidase precursor
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase) [Eschscholzia
           californica]; contains PF01565 FAD binding domain
          Length = 541

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 14/36 (38%), Positives = 21/36 (58%)

Query: 186 VIEGVHMCMVMRGVQKINSKTVTSTMLGVFRDDPKT 221
           +IE +H+ +V+R      +KTVT T LG F  +  T
Sbjct: 291 IIEEIHIRVVLRAAGNDGNKTVTMTYLGQFLGEKGT 326


>At4g34900.1 68417.m04949 xanthine dehydrogenase, putative similar
           to xanthine dehydrogenase from Gallus gallus, PIR:XOCHDH
           [SP|P47990], from Calliphora vicina [SP|P08793];
           contains Pfam profile PF02738 Aldehyde oxidase and
           xanthine dehydrogenase, molybdopterin binding domain
          Length = 1364

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 2/38 (5%)

Query: 109 EMVVVKDIEMFSMCEHHLVPFYGKVSIGYLPQGKILGL 146
           ++++ +D++M  M   H   F GK  +G+  +GKIL L
Sbjct: 856 KLILDRDVDM--MITGHRHSFVGKYKVGFTNEGKILAL 891


>At4g34890.1 68417.m04948 xanthine dehydrogenase, putative similar
           to xanthine dehydrogenase from Gallus gallus, PIR:XOCHDH
           [SP|P47990]; contains Pfam profile PF02738 Aldehyde
           oxidase and xanthine dehydrogenase, molybdopterin
           binding domain
          Length = 1361

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 2/38 (5%)

Query: 109 EMVVVKDIEMFSMCEHHLVPFYGKVSIGYLPQGKILGL 146
           ++++ +D++M  M   H   F GK  +G+  +GKIL L
Sbjct: 853 KLILDRDVDM--MITGHRHSFLGKYKVGFTNEGKILAL 888


>At3g22990.1 68416.m02899 expressed protein
          Length = 460

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 14/50 (28%), Positives = 23/50 (46%)

Query: 49  LPDMANSYRLLLTGLGEDPERAGLLKTPERAAKAMLFFTKGYDQSLEEVL 98
           +P++     ++L  L  +P+  GLL   E A   +LF    Y  S   +L
Sbjct: 391 VPEVCRKAAMILENLVSEPQNRGLLLAYENAFAELLFQEGKYSDSFARIL 440


>At1g69220.1 68414.m07925 serine/threonine protein kinase, putative
           identical to serine/threonine kinase [Arabidopsis
           thaliana] gi|2352084|gb|AAB68776
          Length = 836

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 14/32 (43%), Positives = 22/32 (68%), Gaps = 1/32 (3%)

Query: 197 RGVQKINSKTVTSTML-GVFRDDPKTREEFLN 227
           R +Q+ NSK  T+++   + R+DP T+ EFLN
Sbjct: 222 RKMQQQNSKMSTTSLPDSITREDPTTKYEFLN 253


>At3g60290.1 68416.m06739 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to flavonol synthase 1
           [SP|Q96330], gibberellin 20-oxidase [GI:9791186];
           contains PF03171 2OG-Fe(II) oxygenase superfamily domain
          Length = 316

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 12/39 (30%), Positives = 20/39 (51%)

Query: 96  EVLNNAIFDEDTDEMVVVKDIEMFSMCEHHLVPFYGKVS 134
           EV++N I+      + V KD +  S    H +P + K+S
Sbjct: 233 EVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKIS 271


>At3g01310.1 68416.m00042 expressed protein similar to unknown
           protein GB:BAA24863 [Homo sapiens], unknown protein
           GB:BAA20831 [Homo sapiens], unknown protein GB:AAB42264
           [Caenorhabditis elegans]
          Length = 1056

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 2/41 (4%)

Query: 66  DPERAGLLKTPERAAKAMLFFT-KGYDQSLEEVLNNAIFDE 105
           DP+ A + KTPER  +  L+FT + +  SL  VL     DE
Sbjct: 866 DPKYANV-KTPERHVRTRLYFTSESHIHSLMNVLRYCNLDE 905


>At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to flavonol synthase SP|Q96330
           {Arabidopsis thaliana}, SP|Q07512 {Petunia hybrida};
           contains Pfam profile PF03171: oxidoreductase,
           2OG-Fe(II) oxygenase family
          Length = 357

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 11/39 (28%), Positives = 21/39 (53%)

Query: 96  EVLNNAIFDEDTDEMVVVKDIEMFSMCEHHLVPFYGKVS 134
           EV++N I+      + V K+++  S    H +P + K+S
Sbjct: 274 EVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKIS 312


>At1g19690.1 68414.m02455 expressed protein similar to (SP:P76370)
           Protein yeeZ precursor. {Escherichia coli O157:H7}
          Length = 352

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 3/90 (3%)

Query: 136 GYLPQGKILGLS-KLARIVEIFSRRLQVQERLTKQIAIAVTQAVRPAGVAVVIEGVHMCM 194
           G+L  G+ LG+S ++ R+  I+       + L KQ  ++  Q  R +        VH+  
Sbjct: 192 GWLSLGRDLGVSTQILRLGGIYGPGRSAIDTLLKQERLSEGQKRRAS--RKFTSRVHVED 249

Query: 195 VMRGVQKINSKTVTSTMLGVFRDDPKTREE 224
           + + ++    K  +  +  +  DDP  REE
Sbjct: 250 ICQVLKAATEKPASGEIYNIVDDDPAAREE 279


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.323    0.137    0.399 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,261,485
Number of Sequences: 28952
Number of extensions: 205539
Number of successful extensions: 484
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 466
Number of HSP's gapped (non-prelim): 13
length of query: 232
length of database: 12,070,560
effective HSP length: 79
effective length of query: 153
effective length of database: 9,783,352
effective search space: 1496852856
effective search space used: 1496852856
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 58 (27.5 bits)