BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001219-TA|BGIBMGA001219- PA|IPR002573|Choline/ethanolamine kinase, IPR011009|Protein kinase-like (343 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G7.12c |||choline kinase |Schizosaccharomyces pombe|chr 1|... 62 1e-10 SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 30 0.42 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 29 0.96 SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizo... 28 1.7 SPAC644.06c |cdr1|nim1|GIN4 family protein kinase Cdr1|Schizosac... 28 2.2 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 5.1 SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 26 6.8 SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce... 26 6.8 SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 26 9.0 >SPAC13G7.12c |||choline kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 61.7 bits (143), Expect = 1e-10 Identities = 51/187 (27%), Positives = 84/187 (44%), Gaps = 24/187 (12%) Query: 76 NIKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVEL-- 133 N+K L+ + P L F NG QY TL +T+ D K+ V +++ ++H L Sbjct: 111 NLKRLARHNIGPYLIGEFSNGRFEQYMESTTLTCKTIRDPKLSIYVGRRLCELHNFILLH 170 Query: 134 GKEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSS---------FGSVTKLRIEFERL 184 EV + P W +L K KH+ ++S F + +E+ R Sbjct: 171 PHEVLEMPAAWKNCLVWLPKAKAKILGR-KHSLAITSEFMKTLEEDFNAYYNWFVEWSRD 229 Query: 185 KSHLIKTESPIVFAHNDLLLGNVI----------FNKDEGTISFIDYEYASYNYQAFDIA 234 K + +VF+HND GN++ ++ T+ +D+EYA N AFD+A Sbjct: 230 KKDWFGLK--MVFSHNDTQYGNLLKIKAKKRSIPLSQKHRTLVPVDFEYAGPNLCAFDLA 287 Query: 235 NHFNEFV 241 N+F E++ Sbjct: 288 NYFAEWM 294 >SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 30.3 bits (65), Expect = 0.42 Identities = 22/58 (37%), Positives = 27/58 (46%), Gaps = 10/58 (17%) Query: 41 NVKFKYFVRKGQRSTKPKLAECLFLEVGVHYFLPRNIKTLSARGLAPKLYAVFENGLI 98 N KF V+KG RS P L C RNI ++SA L PK + F GL+ Sbjct: 2 NSKFFGLVQKGTRSFFPSLNFCY----------TRNIMSVSASSLVPKDFRAFRIGLV 49 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 29.1 bits (62), Expect = 0.96 Identities = 27/84 (32%), Positives = 40/84 (47%), Gaps = 3/84 (3%) Query: 122 AKQMAKMHRVELGKEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVS-SFGSVTKLRI- 179 AK +A++ R E K Q P+V +IE+ SLL + V H+R S G KL + Sbjct: 857 AKMVAEVDRAEALKSGQFRPLVRKEIEEHCSLLGLD-AELVSHSRIKGLSGGQKVKLVLA 915 Query: 180 EFERLKSHLIKTESPIVFAHNDLL 203 L+ H+I + P + D L Sbjct: 916 ACTWLRPHVIVLDEPTNYLDRDSL 939 >SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 28.3 bits (60), Expect = 1.7 Identities = 17/59 (28%), Positives = 27/59 (45%) Query: 128 MHRVELGKEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKS 186 +++ L K V KD V ++ EQ L+ L + + E +HN L F + KS Sbjct: 25 LYKEVLNKGVSKDEKVANEQEQALTNLSDLYVRENRHNDLAQLVQQSRPLMANFSKAKS 83 >SPAC644.06c |cdr1|nim1|GIN4 family protein kinase Cdr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 593 Score = 27.9 bits (59), Expect = 2.2 Identities = 20/94 (21%), Positives = 37/94 (39%), Gaps = 1/94 (1%) Query: 197 FAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDSDYQRYPSK 256 F H DL L N++ +E I D+ A+ + N+ L+ E ++ Y Sbjct: 124 FRHRDLKLENILIKVNEQQIKIADFGMATVEPNDSCLENYCGSLHYLAPEIVSHKPYRGA 183 Query: 257 DFQLAWIRVYLSEYLGTMTP-DAKSVDTVYEEVQ 289 + V L L P ++ D +Y +++ Sbjct: 184 PADVWSCGVILYSLLSNKLPFGGQNTDVIYNKIR 217 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 26.6 bits (56), Expect = 5.1 Identities = 15/53 (28%), Positives = 25/53 (47%) Query: 150 FLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESPIVFAHNDL 202 F+ LL E+ + F G VT R + ++L+S + I+F H D+ Sbjct: 1650 FILLLYERLIQCYLNIDFEGKIGVVTPYRSQVQQLRSQFQRKYGSIIFKHLDI 1702 >SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1362 Score = 26.2 bits (55), Expect = 6.8 Identities = 12/30 (40%), Positives = 17/30 (56%) Query: 150 FLSLLPEKFSTEVKHNRFVSSFGSVTKLRI 179 F+ LPE+FST V F+ S G ++ I Sbjct: 549 FIMTLPEQFSTNVGQRGFLMSGGQKQRIAI 578 >SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 906 Score = 26.2 bits (55), Expect = 6.8 Identities = 15/49 (30%), Positives = 26/49 (53%) Query: 147 IEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESPI 195 IE+ + L FS+ + F ++FG V+ F+ L+S ++ ES I Sbjct: 19 IEENIDRLTSSFSSNGELVVFATAFGDVSIYNSSFKSLQSIKVEDESSI 67 >SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 25.8 bits (54), Expect = 9.0 Identities = 11/40 (27%), Positives = 21/40 (52%) Query: 90 YAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMH 129 + V ++ L P + N + L +WPL ++M+K+H Sbjct: 366 WKVLDDSLNEFQSPAENTNDKIELTESMWPLPEEEMSKLH 405 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.322 0.139 0.423 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,677,077 Number of Sequences: 5004 Number of extensions: 72951 Number of successful extensions: 171 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 158 Number of HSP's gapped (non-prelim): 12 length of query: 343 length of database: 2,362,478 effective HSP length: 73 effective length of query: 270 effective length of database: 1,997,186 effective search space: 539240220 effective search space used: 539240220 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 54 (25.8 bits)
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