Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001219-TA|BGIBMGA001219-
PA|IPR002573|Choline/ethanolamine kinase, IPR011009|Protein
kinase-like
(343 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26830.1 68415.m03219 choline/ethanolamine kinase family prot... 153 1e-37
At1g74320.1 68414.m08606 choline kinase, putative similar to GmC... 125 5e-29
At1g71697.1 68414.m08288 choline kinase, putative similar to GmC... 115 5e-26
At4g09760.2 68417.m01603 choline kinase, putative similar to GmC... 113 2e-25
At4g09760.1 68417.m01602 choline kinase, putative similar to GmC... 113 2e-25
At1g34100.1 68414.m04229 choline kinase, putative similar to cho... 108 5e-24
At4g09760.3 68417.m01604 choline kinase, putative similar to GmC... 49 4e-06
At5g67310.1 68418.m08488 cytochrome P450 family protein 30 2.0
At3g30300.1 68416.m03826 expressed protein contains Pfam PF03138... 29 4.5
At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 ... 28 7.9
>At2g26830.1 68415.m03219 choline/ethanolamine kinase family protein
similar to SP|Q9HBU6 Ethanolamine kinase (EC 2.7.1.82)
(EKI){Homo sapiens}, GmCK3p [Glycine max] GI:1438883;
contains Pfam profile PF01633: Choline/ethanolamine
kinase
Length = 374
Score = 153 bits (372), Expect = 1e-37
Identities = 87/253 (34%), Positives = 130/253 (51%), Gaps = 7/253 (2%)
Query: 77 IKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVELGKE 136
IK LSA G KL F NG++ + TL + + KI +A+++ K H+V++
Sbjct: 112 IKYLSAAGFGAKLLGGFGNGMVQSFINARTLEPSDMREPKIAAQIARELGKFHKVDIPGS 171
Query: 137 VQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESPIV 196
K+P +W I +F E + + S +L E L+ +P+V
Sbjct: 172 --KEPQLWVDILKFYEKASTLTFEEPDKQKLFETI-SFEELHKEIIELREFTGLLNAPVV 228
Query: 197 FAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDSDYQRYPSK 256
FAHNDLL GN + N +E + ID+EY SYNY+ FDI NHFNE+ G D DY YPSK
Sbjct: 229 FAHNDLLSGNFMLNDEEEKLYLIDFEYGSYNYRGFDIGNHFNEYAGY---DCDYSLYPSK 285
Query: 257 DFQLAWIRVYLSEYLGTMTPDAKSVDTVYEEVQKLSLASHFLWGIWSLVQFEHSDIDFDF 316
+ Q +I+ YL A+ V++V+ E LASH W IW+++Q S I+F++
Sbjct: 286 EEQYHFIKHYLQPDKPDEVSIAE-VESVFVETDAYKLASHLYWAIWAIIQARMSPIEFEY 344
Query: 317 GSPLWIELGEAKR 329
++ E K+
Sbjct: 345 LGYFFLRYNEYKK 357
>At1g74320.1 68414.m08606 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 350
Score = 125 bits (301), Expect = 5e-29
Identities = 74/246 (30%), Positives = 125/246 (50%), Gaps = 14/246 (5%)
Query: 75 RNIKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVELG 134
R + +S G P L F NG I ++ TL+ + D +I +A +M + H +E+
Sbjct: 90 RTFEFMSKHGHGPLLLGRFGNGRIEEFLHARTLSACDLRDPEISGRIATRMKEFHGLEMP 149
Query: 135 KEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESP 194
K ++WD++ +L+ S E SF + + +E L+ L +
Sbjct: 150 GA--KKALLWDRLRNWLTACKRLASPEE-----AKSF-RLDVMEMEINMLEKSLFDNDEN 201
Query: 195 IVFAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDS----DY 250
I F HNDL GN++ +++ I+ IDYEY+ YN A+DIANHF E ++ DY
Sbjct: 202 IGFCHNDLQYGNIMMDEETKAITIIDYEYSCYNPVAYDIANHFCEMAADYHTETPHIMDY 261
Query: 251 QRYPSKDFQLAWIRVYLSEYLGTMTPDAKSVDTVYEEVQKLSLASHFLWGIWSLVQFEHS 310
+YP + + +++ Y+S P V + E+V+K +LASH +WG+W ++ +
Sbjct: 262 SKYPGVEERQRFLKTYMS--YSDEKPSDTMVKKLLEDVEKYTLASHLIWGLWGIISEHVN 319
Query: 311 DIDFDF 316
+IDFD+
Sbjct: 320 EIDFDY 325
>At1g71697.1 68414.m08288 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 115 bits (276), Expect = 5e-26
Identities = 72/258 (27%), Positives = 132/258 (51%), Gaps = 19/258 (7%)
Query: 68 GVHYFLPRN--IKT---LSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVA 122
GV F R IKT +S G PKL F +G + ++ TL+ + + + +A
Sbjct: 78 GVDLFFNRGDEIKTFECMSHHGYGPKLLGRFSDGRLEEFIHARTLSADDLRVAETSDFIA 137
Query: 123 KQMAKMHRVELGKEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFE 182
++ + H++++ K+ ++W+++ +L + ++ ++ +++ + L E
Sbjct: 138 AKLREFHKLDMPGP--KNVLLWERLRTWLKEA-KNLASPIEMDKY-----RLEGLENEIN 189
Query: 183 RLKSHLIKTESPIVFAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVG 242
L+ L + + I F HNDL GNV+ ++ I+ IDYEY+S+N A+DIANHF E
Sbjct: 190 LLEERLTRDDQEIGFCHNDLQYGNVMIDEVTNAITIIDYEYSSFNPIAYDIANHFCEMAA 249
Query: 243 LSIEDS----DYQRYPSKDFQLAWIRVYLSEYLGTMTPDAKSVDTVYEEVQKLSLASHFL 298
D+ DY YP + + +I YL G T D K V+ + ++ + +LA+H
Sbjct: 250 NYHSDTPHVLDYTLYPGEGERRRFISTYLGS-TGNATSD-KEVERLLKDAESYTLANHIF 307
Query: 299 WGIWSLVQFEHSDIDFDF 316
WG+W ++ + I+FD+
Sbjct: 308 WGLWGIISGHVNKIEFDY 325
>At4g09760.2 68417.m01603 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 113 bits (272), Expect = 2e-25
Identities = 69/248 (27%), Positives = 127/248 (51%), Gaps = 19/248 (7%)
Query: 75 RNIKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVELG 134
R + ++ G P L F G + ++ TL+ + D I LVA ++ + H + +
Sbjct: 88 RTFEYVARHGHGPTLLGRFAGGRVEEFIHARTLSATDLRDPNISALVASKLRRFHSIHIP 147
Query: 135 KEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESP 194
+ + ++WD++ ++ S E H+ + FG + + E L+ + E
Sbjct: 148 GD--RIMLIWDRMRTWVGQAKNLCSNE--HS---TEFG-LDDIEDEINLLEQE-VNNEQE 198
Query: 195 IVFAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDS----DY 250
I F HNDL GN++ +++ I+ IDYEYASYN A+DIANHF E ++ DY
Sbjct: 199 IGFCHNDLQYGNIMIDEETNAITIIDYEYASYNPIAYDIANHFCEMAADYHSNTPHILDY 258
Query: 251 QRYPSKDFQLAWIRVYLSEYLGTMTPDAK--SVDTVYEEVQKLSLASHFLWGIWSLVQFE 308
YP ++ + R ++ YL + +A+ ++ + ++++K +LASH WG+W ++
Sbjct: 259 TLYPGEEER----RRFICNYLTSSGEEAREEDIEQLLDDIEKYTLASHLFWGLWGIISGY 314
Query: 309 HSDIDFDF 316
+ I+FD+
Sbjct: 315 VNKIEFDY 322
>At4g09760.1 68417.m01602 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 113 bits (272), Expect = 2e-25
Identities = 69/248 (27%), Positives = 127/248 (51%), Gaps = 19/248 (7%)
Query: 75 RNIKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVELG 134
R + ++ G P L F G + ++ TL+ + D I LVA ++ + H + +
Sbjct: 88 RTFEYVARHGHGPTLLGRFAGGRVEEFIHARTLSATDLRDPNISALVASKLRRFHSIHIP 147
Query: 135 KEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESP 194
+ + ++WD++ ++ S E H+ + FG + + E L+ + E
Sbjct: 148 GD--RIMLIWDRMRTWVGQAKNLCSNE--HS---TEFG-LDDIEDEINLLEQE-VNNEQE 198
Query: 195 IVFAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDS----DY 250
I F HNDL GN++ +++ I+ IDYEYASYN A+DIANHF E ++ DY
Sbjct: 199 IGFCHNDLQYGNIMIDEETNAITIIDYEYASYNPIAYDIANHFCEMAADYHSNTPHILDY 258
Query: 251 QRYPSKDFQLAWIRVYLSEYLGTMTPDAK--SVDTVYEEVQKLSLASHFLWGIWSLVQFE 308
YP ++ + R ++ YL + +A+ ++ + ++++K +LASH WG+W ++
Sbjct: 259 TLYPGEEER----RRFICNYLTSSGEEAREEDIEQLLDDIEKYTLASHLFWGLWGIISGY 314
Query: 309 HSDIDFDF 316
+ I+FD+
Sbjct: 315 VNKIEFDY 322
>At1g34100.1 68414.m04229 choline kinase, putative similar to
choline kinase (GmCK2p) GI:1438881 from [Glycine max];
contains Pfam profile PF01633: Choline/ethanolamine
kinase; could not find a suitable start codon; this may
be a pseudogene, a transposon insertion is found
immediately upstream.
Length = 267
Score = 108 bits (260), Expect = 5e-24
Identities = 70/244 (28%), Positives = 122/244 (50%), Gaps = 19/244 (7%)
Query: 75 RNIKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVELG 134
R + +S G PKL F G I ++ TL+ + D ++ VA ++ + H + +
Sbjct: 11 RTFEVVSRYGHGPKLLGRFSGGRIEEFINARTLSAANLRDAEVPTRVAAKLREFHGINIP 70
Query: 135 KEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKT--E 192
+ ++ ++WD++ +L + E ++ FG + K+ E L+ L +
Sbjct: 71 GD--RNVLIWDRMRNWLRQAKSLCTPED-----LAEFG-LDKIEDEIYLLEHELQDKCKQ 122
Query: 193 SPIVFAHNDLLLGNVIFNKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDSDYQR 252
I F HNDL GN++ ++D I+ IDYEYASYN A+DIANHF E ++Y +
Sbjct: 123 QEIGFCHNDLQYGNIMIDEDTNAITIIDYEYASYNPVAYDIANHFCEMA------ANY-K 175
Query: 253 YPSKDFQLAWIRVYLSEYLGTMTPDAKSVDTVYEEVQKLSLASHFLWGIWSLVQFEHSDI 312
++ + +I YLS P + + ++ +K +LASH WG+W ++ + I
Sbjct: 176 VAGEEERRRFIHNYLSS--SGEEPKEDDIKQLLKDAEKYTLASHLFWGLWGIISGYVNKI 233
Query: 313 DFDF 316
DFD+
Sbjct: 234 DFDY 237
Score = 28.3 bits (60), Expect = 7.9
Identities = 22/93 (23%), Positives = 43/93 (46%), Gaps = 5/93 (5%)
Query: 140 DPMVWDKIEQFLSLLPE-KFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESPIVFA 198
+P+ +D F + K + E + RF+ ++ S + + + +K L E + +
Sbjct: 157 NPVAYDIANHFCEMAANYKVAGEEERRRFIHNYLSSSGEEPKEDDIKQLLKDAEKYTLAS 216
Query: 199 HNDLLLGNVIFNKDEGTISFIDYEYASYNYQAF 231
H L +I G ++ ID++YA Y+ Q F
Sbjct: 217 HLFWGLWGII----SGYVNKIDFDYAEYSRQRF 245
>At4g09760.3 68417.m01604 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 255
Score = 49.2 bits (112), Expect = 4e-06
Identities = 34/145 (23%), Positives = 67/145 (46%), Gaps = 9/145 (6%)
Query: 75 RNIKTLSARGLAPKLYAVFENGLIYQYFPGDTLNIETVLDIKIWPLVAKQMAKMHRVELG 134
R + ++ G P L F G + ++ TL+ + D I LVA ++ + H + +
Sbjct: 88 RTFEYVARHGHGPTLLGRFAGGRVEEFIHARTLSATDLRDPNISALVASKLRRFHSIHIP 147
Query: 135 KEVQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFVSSFGSVTKLRIEFERLKSHLIKTESP 194
+ + ++WD++ ++ S E H+ + FG + + E L+ + E
Sbjct: 148 GD--RIMLIWDRMRTWVGQAKNLCSNE--HS---TEFG-LDDIEDEINLLEQE-VNNEQE 198
Query: 195 IVFAHNDLLLGNVIFNKDEGTISFI 219
I F HNDL GN++ +++ I+ I
Sbjct: 199 IGFCHNDLQYGNIMIDEETNAITII 223
>At5g67310.1 68418.m08488 cytochrome P450 family protein
Length = 507
Score = 30.3 bits (65), Expect = 2.0
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 3/32 (9%)
Query: 137 VQKDPMVWDKIEQFLSLLPEKFSTEVKHNRFV 168
+Q+DP VWD E F PE+F +E +F+
Sbjct: 408 IQRDPNVWDDPETF---KPERFESETHRGKFL 436
>At3g30300.1 68416.m03826 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 677
Score = 29.1 bits (62), Expect = 4.5
Identities = 21/90 (23%), Positives = 37/90 (41%)
Query: 210 NKDEGTISFIDYEYASYNYQAFDIANHFNEFVGLSIEDSDYQRYPSKDFQLAWIRVYLSE 269
N+ E T+ + ++A D V +S D D + +PS + R+Y S
Sbjct: 456 NEPESTVIELRTNAHKLLWEAIDYVVSVEADVFISGFDRDGKGHPSFASLVMGHRLYQSA 515
Query: 270 YLGTMTPDAKSVDTVYEEVQKLSLASHFLW 299
T PD K + + EE++ ++ W
Sbjct: 516 SAKTFRPDRKQIAMLLEEIRDHMYEANHTW 545
>At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 (4
significant) WD-40 repeats (PF0400); identical to WD-40
repeat protein MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 507
Score = 28.3 bits (60), Expect = 7.9
Identities = 13/44 (29%), Positives = 23/44 (52%)
Query: 122 AKQMAKMHRVELGKEVQKDPMVWDKIEQFLSLLPEKFSTEVKHN 165
++Q + + E GK+ Q+ P V +K Q+ L+P + HN
Sbjct: 43 SQQQSDVKMKESGKKTQQSPSVDEKYSQWKGLVPILYDWLANHN 86
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.322 0.139 0.423
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,356,809
Number of Sequences: 28952
Number of extensions: 352935
Number of successful extensions: 762
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 742
Number of HSP's gapped (non-prelim): 12
length of query: 343
length of database: 12,070,560
effective HSP length: 82
effective length of query: 261
effective length of database: 9,696,496
effective search space: 2530785456
effective search space used: 2530785456
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 60 (28.3 bits)
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