GeneModel BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001212-TA|BGIBMGA001212-PA|IPR002048|Calcium-binding
EF-hand
         (153 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...   119   2e-28
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    83   1e-17
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    61   6e-11
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    52   5e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    46   3e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    42   4e-05
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    36   0.003
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    31   0.10 
SPBC2G2.03c |sbh1||translocon beta subunit Sbh1|Schizosaccharomy...    25   5.1  
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo...    25   5.1  
SPBC1289.06c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    25   6.7  
SPAC26A3.14c ||SPAC23A6.14c|DUF1748 family protein|Schizosacchar...    25   6.7  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    24   8.8  

>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score =  119 bits (287), Expect = 2e-28
 Identities = 61/139 (43%), Positives = 88/139 (63%), Gaps = 1/139 (0%)

Query: 7   AVATIQKKTATTNPGPGGVRKKSGPKFELTEEQKRDIKEAFDLFDTEKTGKIDTKELKVA 66
           A A  ++++  ++P P  +   +  + E+TEEQ++DI EAF LFD++K   ID  EL+ A
Sbjct: 3   ANARAKRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAA 62

Query: 67  IRALGFEPXXXXXXXXXXXXX-XGDGKVSFDDFMELMSVKMAEKDTREEIMKAFKLFDDD 125
           +RALGF                 G G +  +DF+ +M+ K+ E+D  EEI +AF+LFDDD
Sbjct: 63  MRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDD 122

Query: 126 ETGKISFKNLKRVAKELGE 144
           ETGKIS +NL+RVAKEL E
Sbjct: 123 ETGKISLRNLRRVAKELNE 141



 Score = 33.5 bits (73), Expect = 0.014
 Identities = 14/45 (31%), Positives = 26/45 (57%)

Query: 109 KDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGEISQMKNCMK 153
           ++ R++I +AFKLFD D+   I +  L+   + LG  ++    +K
Sbjct: 33  EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLK 77


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 83.4 bits (197), Expect = 1e-17
 Identities = 42/111 (37%), Positives = 62/111 (55%), Gaps = 1/111 (0%)

Query: 35  LTEEQKRDIKEAFDLFDTEKTGKIDTKELKVAIRALGFEPXXXXXXXXXXXXXX-GDGKV 93
           LT+EQ  + +EAF LFD ++ G I + EL V +R+LG  P               G+G +
Sbjct: 6   LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65

Query: 94  SFDDFMELMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGE 144
            F +F+ +M+ KM + D  EE+ +AFK+FD D  G I+ + L  V   LGE
Sbjct: 66  DFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116



 Score = 25.8 bits (54), Expect = 2.9
 Identities = 11/50 (22%), Positives = 28/50 (56%), Gaps = 1/50 (2%)

Query: 89  GDGKVSFDDFMELMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRV 138
           G+G ++ ++   +++  + E+ ++EE+    +  D D  G I+++   RV
Sbjct: 98  GNGYITVEELTHVLT-SLGERLSQEEVADMIREADTDGDGVINYEEFSRV 146


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 61.3 bits (142), Expect = 6e-11
 Identities = 36/109 (33%), Positives = 54/109 (49%), Gaps = 3/109 (2%)

Query: 36  TEEQKRDIKEAFDLFDTEKTGKIDTKELKVAIRALGFEPXXXXXXXXXXXXXXGDGKVSF 95
           ++EQ  ++KEAF L+D +K G I T  +   +R+LG                 GD  +  
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGIN--VTDAELAKLSNELGDA-IDE 60

Query: 96  DDFMELMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGE 144
             FM  +S K+ E ++ EE +KAF++FD D +G I         K LGE
Sbjct: 61  KKFMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGE 109



 Score = 28.3 bits (60), Expect = 0.54
 Identities = 13/37 (35%), Positives = 22/37 (59%)

Query: 107 AEKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELG 143
           A K+  +E+ +AF L+D D+ G I   ++  V + LG
Sbjct: 3   ASKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLG 39


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 51.6 bits (118), Expect = 5e-08
 Identities = 28/119 (23%), Positives = 54/119 (45%), Gaps = 3/119 (2%)

Query: 26  RKKSGPKFELTEEQKRDIKEAFDLFDTEKTGKIDTKELKVAIRALGFEPXXXXXXXXXXX 85
           R  SG   +LT  Q +++KEAF L D +  G I  +++K  + +L  +            
Sbjct: 33  RASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFES 92

Query: 86  XXXGDGKVSFDDFMELMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGE 144
               +  ++   F+  M   +     R ++++AF  FDD ++GKI    ++     +G+
Sbjct: 93  I---NPPINLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGD 148


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 46.0 bits (104), Expect = 3e-06
 Identities = 30/103 (29%), Positives = 45/103 (43%), Gaps = 5/103 (4%)

Query: 44  KEAFDLFDTEKTGKIDTKELKVAIRALGFEPXXXXXXXXXXXXXXGDGKVSFDDFMELMS 103
           K+AF LFD   TG+I    +   +RA G  P                 +V  + F+++++
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL---PAEVDMEQFLQVLN 65

Query: 104 VKMA--EKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGE 144
                      EE +K F++FD D TG I    L+ V   LGE
Sbjct: 66  RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGE 108


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 41.9 bits (94), Expect = 4e-05
 Identities = 29/105 (27%), Positives = 47/105 (44%), Gaps = 3/105 (2%)

Query: 36  TEEQKRDIKEAFDLFDTEKTGKIDTKELKVAIRALGFEPXXXXXXXXXXXXXXGDGKVSF 95
           + E+   I++ F   D  ++G ID  E  ++I ++   P              GD  V F
Sbjct: 19  SNEEIERIRKRFIKIDANQSGSIDRNEF-LSIPSVASNPLASRLFSVVDEDGGGD--VDF 75

Query: 96  DDFMELMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRVAK 140
            +F+  +SV     +  E++  AFK++D D  G IS   L  V K
Sbjct: 76  QEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLK 120



 Score = 24.6 bits (51), Expect = 6.7
 Identities = 12/48 (25%), Positives = 20/48 (41%)

Query: 101 LMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGEISQM 148
           ++   + E   ++ + K     D D  GKISF+  K +       S M
Sbjct: 122 MVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKDIVSGSNVTSSM 169


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 35.9 bits (79), Expect = 0.003
 Identities = 22/64 (34%), Positives = 31/64 (48%), Gaps = 1/64 (1%)

Query: 38  EQKRDIKEAFDLFDTEKTGKIDTKELKVAIRALGFEPXXXXXXXXXXXXXXGDGKVSFDD 97
           E   + +EAFDLFD    G ID ++L+ +   LG E                +GKVS ++
Sbjct: 9   EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLG-ENLTKEQLQLMLDLAGTNGKVSREE 67

Query: 98  FMEL 101
           F EL
Sbjct: 68  FAEL 71



 Score = 35.1 bits (77), Expect = 0.005
 Identities = 18/43 (41%), Positives = 24/43 (55%)

Query: 102 MSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRVAKELGE 144
           MSV     +  EE  +AF LFD    G I F++L+R   +LGE
Sbjct: 1   MSVSTKRLEMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGE 43


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 30.7 bits (66), Expect = 0.10
 Identities = 14/49 (28%), Positives = 30/49 (61%), Gaps = 1/49 (2%)

Query: 90  DGKVSFDDFMELMSVKMAEKDTREEIMKAFKLFDDDETGKISFKNLKRV 138
           +G + F +F+  +SV  +  +  ++++ AF+L+D D  G IS+  + R+
Sbjct: 77  NGYIDFKEFICALSVT-SRGELNDKLIWAFQLYDLDNNGLISYDEMLRI 124



 Score = 29.5 bits (63), Expect = 0.24
 Identities = 24/99 (24%), Positives = 46/99 (46%), Gaps = 12/99 (12%)

Query: 47  FDLFDTEKTGKIDTKELKVAIRALG-FEPXXXXXXXXXXXXXXGDGKVSFDDFMELMS-- 103
           F++FD +K G ID KE   A+      E                +G +S+D+ + ++   
Sbjct: 69  FNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEMLRIVDAI 128

Query: 104 -------VKMAE-KDTREE-IMKAFKLFDDDETGKISFK 133
                  VK+ E +DT E+ + K F + D ++ G+++ +
Sbjct: 129 YKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLTLE 167


>SPBC2G2.03c |sbh1||translocon beta subunit
          Sbh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 102

 Score = 25.0 bits (52), Expect = 5.1
 Identities = 9/23 (39%), Positives = 12/23 (52%)

Query: 9  ATIQKKTATTNPGPGGVRKKSGP 31
          A ++K T  +N GP G R    P
Sbjct: 29 AAVEKNTKESNSGPAGARAAGAP 51


>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
          |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 25.0 bits (52), Expect = 5.1
 Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 2/69 (2%)

Query: 3  LQATAVATIQKKTATTN-PGPGGVRKKSGPKFELTEEQKRDIKEAF-DLFDTEKTGKIDT 60
          LQ   V T  K+  +T+ P       +S PK  +  + K+  +    D F++ K  +IDT
Sbjct: 20 LQNFGVNTAGKENESTSLPRLPKSEDESIPKQSIKSKNKKKTQHFLSDAFESLKRQEIDT 79

Query: 61 KELKVAIRA 69
           E +V + A
Sbjct: 80 NEKEVIVSA 88


>SPBC1289.06c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 481

 Score = 24.6 bits (51), Expect = 6.7
 Identities = 13/30 (43%), Positives = 16/30 (53%)

Query: 38  EQKRDIKEAFDLFDTEKTGKIDTKELKVAI 67
           E+ RDIK+A D   T      D KE K +I
Sbjct: 337 ERDRDIKKATDFITTFINKMSDVKEFKPSI 366


>SPAC26A3.14c ||SPAC23A6.14c|DUF1748 family
          protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 73

 Score = 24.6 bits (51), Expect = 6.7
 Identities = 9/27 (33%), Positives = 16/27 (59%)

Query: 24 GVRKKSGPKFELTEEQKRDIKEAFDLF 50
          G+R+ SG  FE+ +    D+K A + +
Sbjct: 23 GIRRSSGISFEVEKIHNEDVKTAVEKY 49


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with
           EF hand and WH2 motif |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1794

 Score = 24.2 bits (50), Expect = 8.8
 Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 1/33 (3%)

Query: 26  RKKSGPKFELTEEQKRDIKEAFDLFDTEKTGKI 58
           +KK  P + +++E+KR   + FD +D E+ G +
Sbjct: 809 QKKDIP-WAISKEEKRIYDQIFDAWDKERKGTL 840


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.313    0.131    0.353 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 567,268
Number of Sequences: 5004
Number of extensions: 19473
Number of successful extensions: 80
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 49
Number of HSP's gapped (non-prelim): 20
length of query: 153
length of database: 2,362,478
effective HSP length: 67
effective length of query: 86
effective length of database: 2,027,210
effective search space: 174340060
effective search space used: 174340060
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 50 (24.2 bits)