BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001201-TA|BGIBMGA001201-PA|IPR003247|Calponin-like actin-binding subtype, IPR001715|Calponin-like actin-binding, IPR000557|Calponin repeat, IPR003096|SM22/calponin (184 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 69 6e-13 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 67 1e-12 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 31 0.078 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 27 2.2 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 26 3.9 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 26 3.9 SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 25 6.7 SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomy... 25 6.7 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 68.5 bits (160), Expect = 6e-13 Identities = 50/162 (30%), Positives = 80/162 (49%), Gaps = 3/162 (1%) Query: 20 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 79 EKEA+EWIE L K D L+ G +LC++ + ++ + + F MENI Sbjct: 6 EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64 Query: 80 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 138 + F A + VP D+FQT DL+E+++ QV+ ++ + R + LGPK A Sbjct: 65 SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124 Query: 139 DECKRDFS-DEVLKAGQTVIGLQAGSNKGATQSGQNLGAGRK 179 ++ R FS + + + V LQ GS TQ + + R+ Sbjct: 125 EKKPRVFSAQQQREFREGVNSLQYGSFDMPTQGTEKIAFSRR 166 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 67.3 bits (157), Expect = 1e-12 Identities = 35/98 (35%), Positives = 55/98 (56%), Gaps = 1/98 (1%) Query: 22 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGG-QFKMMENIT 80 EA++WIE LG P FE L++G VL L+ K +P + KI + QF+ +NI Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105 Query: 81 NFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118 F I G+P+I F+ D++E K++ +V+ + AL Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 31.5 bits (68), Expect = 0.078 Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 7/101 (6%) Query: 22 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 81 EA++W+E ++ + F D L +G VLCQL K P + ++++ E T Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125 Query: 82 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118 + +A + G+ F+T DL + +I +V+ L AL Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 26.6 bits (56), Expect = 2.2 Identities = 13/37 (35%), Positives = 17/37 (45%) Query: 130 GPCLGPKPADECKRDFSDEVLKAGQTVIGLQAGSNKG 166 GP + K + K+ D V K G V G + SN G Sbjct: 343 GPLISEKAISKVKQHVEDAVQKGGVVVTGGKVASNLG 379 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 25.8 bits (54), Expect = 3.9 Identities = 31/108 (28%), Positives = 47/108 (43%), Gaps = 16/108 (14%) Query: 16 NPEKEKEAQEWIEGVL------GAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV--- 63 N E+ +E + I VL G++ P E FE D KDG +L +LIN P ++ Sbjct: 111 NEEERREFIKHINSVLAGDPDVGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDER 170 Query: 64 ----PKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKD 107 + N FK +EN ++ KA G I D+ E ++ Sbjct: 171 VLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISITNIGAGDILEGRE 218 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 25.8 bits (54), Expect = 3.9 Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 2/41 (4%) Query: 4 ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDV 44 ER+ AK A+K EK+K + ++ EG G + PP E+V Sbjct: 665 EREA-AKAATKSEAEKQKPSGKFEEGT-GGRAPPPPPAEEV 703 >SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 1052 Score = 25.0 bits (52), Expect = 6.7 Identities = 14/45 (31%), Positives = 23/45 (51%), Gaps = 2/45 (4%) Query: 39 ELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQ 83 E + +LK +C I +LKPGS K+++ E+ T F+ Sbjct: 165 EFYMHLLKHMVAVC--IAELKPGSCSKVSSPVSAITADESATGFR 207 >SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 278 Score = 25.0 bits (52), Expect = 6.7 Identities = 18/71 (25%), Positives = 31/71 (43%), Gaps = 1/71 (1%) Query: 2 SLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPG 61 S++ + + ++A K K + E +K P E V+K+ V + +K KP Sbjct: 175 SVKAKKKLRLAEKPASNNSKAGKSSQESKKSSK-APAESAAAVIKEDKVSDRKKSKKKPK 233 Query: 62 SVPKINTTGGQ 72 P N+T Q Sbjct: 234 KTPVSNSTASQ 244 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.315 0.134 0.394 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 849,602 Number of Sequences: 5004 Number of extensions: 33598 Number of successful extensions: 55 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 48 Number of HSP's gapped (non-prelim): 8 length of query: 184 length of database: 2,362,478 effective HSP length: 69 effective length of query: 115 effective length of database: 2,017,202 effective search space: 231978230 effective search space used: 231978230 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits) S2: 51 (24.6 bits)
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