BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001201-TA|BGIBMGA001201-PA|IPR003247|Calponin-like actin-binding subtype, IPR001715|Calponin-like actin-binding, IPR000557|Calponin repeat, IPR003096|SM22/calponin (184 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_40413| Best HMM Match : No HMM Matches (HMM E-Value=.) 114 5e-26 SB_40412| Best HMM Match : No HMM Matches (HMM E-Value=.) 101 4e-22 SB_18673| Best HMM Match : CH (HMM E-Value=2.5e-05) 84 8e-17 SB_7905| Best HMM Match : CH (HMM E-Value=1.3e-10) 75 3e-14 SB_39070| Best HMM Match : No HMM Matches (HMM E-Value=.) 70 1e-12 SB_20280| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 1e-08 SB_23839| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 1e-06 SB_18675| Best HMM Match : NUMOD3 (HMM E-Value=8) 49 3e-06 SB_36121| Best HMM Match : CH (HMM E-Value=0.0017) 45 3e-05 SB_39072| Best HMM Match : No HMM Matches (HMM E-Value=.) 42 2e-04 SB_28994| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.001 SB_53975| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.001 SB_17658| Best HMM Match : GAS2 (HMM E-Value=6.9e-09) 39 0.003 SB_4896| Best HMM Match : TFIID_20kDa (HMM E-Value=2.3e-05) 36 0.026 SB_7838| Best HMM Match : Filamin (HMM E-Value=1.1e-22) 33 0.19 SB_2620| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.25 SB_595| Best HMM Match : CH (HMM E-Value=0) 31 0.43 SB_58953| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.0 SB_15403| Best HMM Match : CH (HMM E-Value=0) 29 3.0 SB_31112| Best HMM Match : Dynein_heavy (HMM E-Value=0) 28 5.3 SB_29928| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.3 SB_42674| Best HMM Match : DUF26 (HMM E-Value=1) 28 5.3 >SB_40413| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 603 Score = 114 bits (274), Expect = 5e-26 Identities = 77/183 (42%), Positives = 105/183 (57%), Gaps = 11/183 (6%) Query: 7 VRAKIASKRNPEKEKEAQEWIEGVLGAKF--PPG--ELFEDVLKDGTVLCQLINKLKPGS 62 V+A I SK +P KE+EA++WI+ VLG G + LKDG VLC+L N+L G Sbjct: 420 VKAGIDSKYDPTKEEEARKWIDTVLGESIFGENGGVDTVHQTLKDGQVLCRLANRL--GG 477 Query: 63 VPKINTTGGQFKMMENITNFQSAIKA-YGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRE 121 KIN FK MENI NF S I+ GV D+FQTVDL+EK ++ V+ + A+GR Sbjct: 478 DLKINQQKMPFKQMENIGNFLSFIENNLGVAKNDLFQTVDLYEKSNMWNVICCIHAVGRR 537 Query: 122 TYRHAEWSGPCLGPKPADECKRDFSDEVLKAGQTVI-GLQAG--SNKGATQSGQNLGAGR 178 Y + P LGPK + + R F++ L G+T+I Q G + AT SGQ+ G R Sbjct: 538 AYSLGK-DVPQLGPKESTKNPRQFTERQLNEGKTIINSFQMGPAAKNVATASGQSFGRQR 596 Query: 179 KIL 181 +I+ Sbjct: 597 QII 599 >SB_40412| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 592 Score = 101 bits (242), Expect = 4e-22 Identities = 60/146 (41%), Positives = 87/146 (59%), Gaps = 7/146 (4%) Query: 13 SKRNPEKEKEAQEWIEGVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPKINT 68 +K + + +A WIEG+LG + G+ D VLKDG VLC++ NKL G KIN+ Sbjct: 62 AKYDSDLAAQATAWIEGILGERVFGGKTGADDVHEVLKDGQVLCRVANKL--GGNIKINS 119 Query: 69 TGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEW 128 + FKMMEN F GVP D+FQTVDL+EK+++ V++ + ALGR+ + + Sbjct: 120 SKMAFKMMENTGKFLEFCDTIGVPKTDMFQTVDLYEKQNMPGVINGIHALGRKAHSTGK- 178 Query: 129 SGPCLGPKPADECKRDFSDEVLKAGQ 154 + LGPK A R+F++E +AGQ Sbjct: 179 TCLALGPKEASANPREFTEEQRRAGQ 204 >SB_18673| Best HMM Match : CH (HMM E-Value=2.5e-05) Length = 195 Score = 83.8 bits (198), Expect = 8e-17 Identities = 61/152 (40%), Positives = 83/152 (54%), Gaps = 17/152 (11%) Query: 10 KIASKRNPEKEKEAQEWIEGVLGAK-FPPGELFEDV---LKDGTVLCQLINKLKPGSVPK 65 KI K +P++E+EA+ WIE VLG K F E +DV LKDG +L +L KL G+ K Sbjct: 17 KILEKYDPQQEEEARIWIEAVLGEKVFGGAEGPDDVQKVLKDGKILARLAIKL--GAKIK 74 Query: 66 INTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRH 125 +N FK MENI NF S GV D FQT DL+ D A + S + Sbjct: 75 VNEQNMPFKQMENIGNFLSHCGHLGVASGDQFQTADLY---DNANMTSCQLIKDLDI--- 128 Query: 126 AEWSGPCLGPKPADECKRDFSDEVLKAGQTVI 157 P LGPK A+ R+F++E L+AG++++ Sbjct: 129 -----PTLGPKEAEANVREFTEEQLRAGESIL 155 >SB_7905| Best HMM Match : CH (HMM E-Value=1.3e-10) Length = 172 Score = 75.4 bits (177), Expect = 3e-14 Identities = 42/116 (36%), Positives = 63/116 (54%), Gaps = 5/116 (4%) Query: 1 MSLERQVRAKIASKRNP---EKEKEAQEWIEGVLGAKF--PPGELFEDVLKDGTVLCQLI 55 +S E +K + P ++ K + WIEG F + F++ LK G VLC+L Sbjct: 45 VSKENLKTSKFIMSKKPWPMDRAKVVRMWIEGKTSCNFGGDTADDFQNTLKSGVVLCKLA 104 Query: 56 NKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQV 111 N ++PG++ KIN F MMENI NF + ++ GV F T+DL+E K++ QV Sbjct: 105 NAIQPGAIKKINNAKMNFMMMENIENFCNFVQTKGVASQYQFVTIDLFEGKNMHQV 160 >SB_39070| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 539 Score = 70.1 bits (164), Expect = 1e-12 Identities = 37/118 (31%), Positives = 64/118 (54%), Gaps = 10/118 (8%) Query: 4 ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFP---------PGELFEDVLKDGTVLCQL 54 E+ ++ +K E E A+ W+E + G PGE F + L+ G +LC++ Sbjct: 259 EKNNVGQVDAKYPKELEGNARRWVETMYGDTVAWGEERPNQRPGETFAEPLRSGVILCKI 318 Query: 55 INKLKPGSVPKINTTGGQFKMMENITNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQV 111 NK+K ++PKI + F M ENI+ F + G+ +++FQTVDL+E++++ V Sbjct: 319 ANKIKSNAIPKIGESNKSFVMQENISKFLDFCERVLGLDRLNLFQTVDLFERQNVGMV 376 Score = 37.5 bits (83), Expect = 0.007 Identities = 22/59 (37%), Positives = 30/59 (50%), Gaps = 9/59 (15%) Query: 3 LERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFP---------PGELFEDVLKDGTVLC 52 L +V KI SK + E E + ++W+E +LG PGE F D + DG VLC Sbjct: 17 LTAEVNRKIQSKYDTELEMQCRDWLEKMLGENIEWGVETEYSRPGESFADGIFDGIVLC 75 Score = 37.1 bits (82), Expect = 0.009 Identities = 21/64 (32%), Positives = 39/64 (60%), Gaps = 6/64 (9%) Query: 54 LINKLKPGSVPKIN-TTGGQF---KMMENITNFQSAIKA--YGVPDIDVFQTVDLWEKKD 107 ++N++ PG++ KI+ GG+F +++ENI F A + + +D+F DL+EK + Sbjct: 203 VMNEVFPGAISKIHGINGGRFHGFQILENIEKFLKACQGEPFNCNVVDLFSPGDLYEKNN 262 Query: 108 IAQV 111 + QV Sbjct: 263 VGQV 266 >SB_20280| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 263 Score = 56.8 bits (131), Expect = 1e-08 Identities = 43/125 (34%), Positives = 67/125 (53%), Gaps = 11/125 (8%) Query: 5 RQVRAKIASKRNPEKEKEAQEWIE--GVLGAKF----PPGELFE--DVLKDGTVLCQLIN 56 R A +S R+ E+ K A++W+ GVL A P LF+ L+DG +LCQ+ N Sbjct: 7 RTASALSSSGRDVEEWKMARDWMISIGVLPAHGRVTDPNFTLFDFAQSLRDGVLLCQVAN 66 Query: 57 KLKPGSVPKINTTG--GQFKMMENITNFQSA-IKAYGVPDIDVFQTVDLWEKKDIAQVVS 113 L PG+V + QF ++NI NF +A + + + D D+F +L++ D +VV+ Sbjct: 67 VLHPGAVVDVGMKPQMSQFMCLKNIRNFLTACTRLFNLADGDLFDANELYDVSDFGKVVN 126 Query: 114 TLFAL 118 TL L Sbjct: 127 TLSKL 131 >SB_23839| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 267 Score = 50.0 bits (114), Expect = 1e-06 Identities = 25/55 (45%), Positives = 38/55 (69%), Gaps = 2/55 (3%) Query: 56 NKLKPGSVPKINTTGGQ--FKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDI 108 N ++PG + K + G+ FKMMENI F + IK+YGV + +F TVDL+EK+++ Sbjct: 189 NAIQPGVIKKPHLNPGKMGFKMMENIGWFVNFIKSYGVQEEYIFVTVDLYEKRNV 243 >SB_18675| Best HMM Match : NUMOD3 (HMM E-Value=8) Length = 89 Score = 48.8 bits (111), Expect = 3e-06 Identities = 30/83 (36%), Positives = 46/83 (55%), Gaps = 12/83 (14%) Query: 74 KMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCL 133 + MENI NF ++ GV +D+FQTVDL+EK+++A R + P L Sbjct: 16 RKMENIGNFLLFCESLGVSKVDLFQTVDLYEKQNMAAA------------RAKGLNCPQL 63 Query: 134 GPKPADECKRDFSDEVLKAGQTV 156 GPK A+ R F ++ L+AG+ + Sbjct: 64 GPKEAEANPRSFDEDKLRAGKGI 86 >SB_36121| Best HMM Match : CH (HMM E-Value=0.0017) Length = 209 Score = 45.2 bits (102), Expect = 3e-05 Identities = 28/83 (33%), Positives = 42/83 (50%), Gaps = 1/83 (1%) Query: 22 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 81 EA+ WIE F + F + L DG +LC+LI + SV +IN G +N++ Sbjct: 32 EARRWIERATFTPFKNDD-FRESLADGVLLCELIVNVANISVGRINKQGTAHAGRDNLSV 90 Query: 82 FQSAIKAYGVPDIDVFQTVDLWE 104 F A +A G+ +F+ DL E Sbjct: 91 FFRACEALGLDKYQLFEHEDLDE 113 >SB_39072| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1011 Score = 42.3 bits (95), Expect = 2e-04 Identities = 22/66 (33%), Positives = 36/66 (54%), Gaps = 2/66 (3%) Query: 62 SVPKINTTGGQFKMMENITNFQSAIKA--YGVPDIDVFQTVDLWEKKDIAQVVSTLFALG 119 + P N M ENI NF +A + + D+FQTV L+E++++ QV+S + A Sbjct: 884 NTPSTNRKSTSLGMQENIANFLNACQEEPFNCNPQDLFQTVYLFERQNLGQVISGIQAFA 943 Query: 120 RETYRH 125 R+ +H Sbjct: 944 RKVRKH 949 >SB_28994| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 422 Score = 40.3 bits (90), Expect = 0.001 Identities = 25/80 (31%), Positives = 39/80 (48%), Gaps = 6/80 (7%) Query: 45 LKDGTVLCQLINKLKPGSVPKINTTGG------QFKMMENITNFQSAIKAYGVPDIDVFQ 98 L DG VLC L+N G++P I+ K M+N+ F A K GV + Sbjct: 336 LADGVVLCHLVNSAYKGTIPSIHIPSAGVPKLTMTKCMKNVDYFLEACKKLGVDRELLCS 395 Query: 99 TVDLWEKKDIAQVVSTLFAL 118 + D+ ++K +V +T+ AL Sbjct: 396 SADILQEKSPQRVCATVQAL 415 >SB_53975| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 43 Score = 39.9 bits (89), Expect = 0.001 Identities = 20/42 (47%), Positives = 29/42 (69%), Gaps = 1/42 (2%) Query: 11 IASKRNPEKEKEAQEWIEGVLGAKFPPGE-LFEDVLKDGTVL 51 +++K +P + EA +WIE VLG K G+ + EDVLKDG +L Sbjct: 1 MSAKYDPVMDAEAVDWIEKVLGEKVCQGKSINEDVLKDGQIL 42 >SB_17658| Best HMM Match : GAS2 (HMM E-Value=6.9e-09) Length = 959 Score = 38.7 bits (86), Expect = 0.003 Identities = 36/130 (27%), Positives = 56/130 (43%), Gaps = 23/130 (17%) Query: 17 PEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKP---------------G 61 P KE A WI +LG + + F +L G VLC+L N ++ G Sbjct: 27 PLKEDLAS-WISRLLGEEELNTDTFTSMLDTGVVLCRLANFIQTVGEEFFVRNPKFPRRG 85 Query: 62 SVPKINTT-------GGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVST 114 P T G F +N++NF + VPD+ +F+T DL K+ V+ T Sbjct: 86 LFPACGVTYKQRGATHGSFVARDNVSNFIRWCRELRVPDVIMFETEDLVLNKNEKTVLLT 145 Query: 115 LFALGRETYR 124 L + R+ ++ Sbjct: 146 LLEVARKAFK 155 >SB_4896| Best HMM Match : TFIID_20kDa (HMM E-Value=2.3e-05) Length = 819 Score = 35.5 bits (78), Expect = 0.026 Identities = 17/36 (47%), Positives = 22/36 (61%) Query: 76 MENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQV 111 M I F +GV D+FQTVDL+EK++I QV Sbjct: 700 MVTIAKFLDFCGTFGVAKSDLFQTVDLYEKQNIQQV 735 Score = 27.9 bits (59), Expect = 5.3 Identities = 11/27 (40%), Positives = 19/27 (70%) Query: 6 QVRAKIASKRNPEKEKEAQEWIEGVLG 32 ++ K+++K +P + EA +WIE VLG Sbjct: 671 ELSRKMSAKYDPVMDAEAVDWIEKVLG 697 >SB_7838| Best HMM Match : Filamin (HMM E-Value=1.1e-22) Length = 820 Score = 32.7 bits (71), Expect = 0.19 Identities = 15/52 (28%), Positives = 25/52 (48%) Query: 41 FEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVP 92 F+ +DG +C+L+N L+PG +P Q+A A+G+P Sbjct: 449 FDTDWRDGMAICELVNALQPGLIPDSKYKEKTNSEATAKIGIQTAHDAFGIP 500 >SB_2620| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 37 Score = 32.3 bits (70), Expect = 0.25 Identities = 12/27 (44%), Positives = 21/27 (77%) Query: 131 PCLGPKPADECKRDFSDEVLKAGQTVI 157 P LGPK A+ R+F++E L+AG++++ Sbjct: 10 PTLGPKEAEANVREFTEEQLRAGESIL 36 >SB_595| Best HMM Match : CH (HMM E-Value=0) Length = 905 Score = 31.5 bits (68), Expect = 0.43 Identities = 27/88 (30%), Positives = 40/88 (45%), Gaps = 3/88 (3%) Query: 19 KEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMM-E 77 +EK + WI LG K L+ D L DG +L QL ++++PG V FK M Sbjct: 674 EEKTFRNWINS-LGIKPFVQNLYLD-LDDGMILFQLFDQVQPGIVNYDKVNKPPFKKMGA 731 Query: 78 NITNFQSAIKAYGVPDIDVFQTVDLWEK 105 + ++ A V D+ F V + K Sbjct: 732 KMKKLENCNYAISVADLLKFSLVGVGGK 759 >SB_58953| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 377 Score = 28.7 bits (61), Expect = 3.0 Identities = 13/26 (50%), Positives = 17/26 (65%) Query: 133 LGPKPADECKRDFSDEVLKAGQTVIG 158 LGP P+D+ RDF L++GQ V G Sbjct: 233 LGPDPSDKKVRDFIWNTLQSGQVVPG 258 >SB_15403| Best HMM Match : CH (HMM E-Value=0) Length = 1907 Score = 28.7 bits (61), Expect = 3.0 Identities = 12/25 (48%), Positives = 19/25 (76%), Gaps = 1/25 (4%) Query: 39 ELFEDVLKDGTVLCQLINKLKPGSV 63 +L+E V KDG +LC++IN PG++ Sbjct: 78 DLYEKV-KDGILLCKMINLSAPGTI 101 >SB_31112| Best HMM Match : Dynein_heavy (HMM E-Value=0) Length = 2532 Score = 27.9 bits (59), Expect = 5.3 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%) Query: 26 WIEGVLGAKF--PPGELFEDVLKDGTVLCQLINKLKPGSVP 64 ++ G +G K+ PP FE +L+ T +I L PGS P Sbjct: 1904 FVTGRMGEKYVTPPVLSFESILEQSTPFSPIIFILSPGSDP 1944 >SB_29928| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 297 Score = 27.9 bits (59), Expect = 5.3 Identities = 13/43 (30%), Positives = 21/43 (48%) Query: 28 EGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTG 70 +G LGA F P +LF + + + ++N G V + TG Sbjct: 73 QGSLGANFDPSKLFYGYMANLNIWANVLNDFNRGKVYEQGCTG 115 >SB_42674| Best HMM Match : DUF26 (HMM E-Value=1) Length = 493 Score = 27.9 bits (59), Expect = 5.3 Identities = 24/86 (27%), Positives = 36/86 (41%), Gaps = 4/86 (4%) Query: 31 LGAKFPPGELFEDV--LKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQSAIKA 88 L A PP DV L GT L LKP PK+ G + + +I F S++ A Sbjct: 89 LMATLPPTSTAHDVERLSTGTKFA-LRQWLKPIVAPKVCNNTGLHRPVAHIPPFTSSVAA 147 Query: 89 YGVPDIDVFQTVDLWEKKDIAQVVST 114 G I + W D ++++ + Sbjct: 148 QGKAPIKSVSALS-WPGTDQSEIMDS 172 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.315 0.134 0.394 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,319,180 Number of Sequences: 59808 Number of extensions: 250668 Number of successful extensions: 374 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 18 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 337 Number of HSP's gapped (non-prelim): 28 length of query: 184 length of database: 16,821,457 effective HSP length: 78 effective length of query: 106 effective length of database: 12,156,433 effective search space: 1288581898 effective search space used: 1288581898 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits) S2: 57 (27.1 bits)
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