SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001201-TA|BGIBMGA001201-PA|IPR003247|Calponin-like
actin-binding subtype, IPR001715|Calponin-like actin-binding,
IPR000557|Calponin repeat, IPR003096|SM22/calponin
         (184 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_40413| Best HMM Match : No HMM Matches (HMM E-Value=.)             114   5e-26
SB_40412| Best HMM Match : No HMM Matches (HMM E-Value=.)             101   4e-22
SB_18673| Best HMM Match : CH (HMM E-Value=2.5e-05)                    84   8e-17
SB_7905| Best HMM Match : CH (HMM E-Value=1.3e-10)                     75   3e-14
SB_39070| Best HMM Match : No HMM Matches (HMM E-Value=.)              70   1e-12
SB_20280| Best HMM Match : No HMM Matches (HMM E-Value=.)              57   1e-08
SB_23839| Best HMM Match : No HMM Matches (HMM E-Value=.)              50   1e-06
SB_18675| Best HMM Match : NUMOD3 (HMM E-Value=8)                      49   3e-06
SB_36121| Best HMM Match : CH (HMM E-Value=0.0017)                     45   3e-05
SB_39072| Best HMM Match : No HMM Matches (HMM E-Value=.)              42   2e-04
SB_28994| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.001
SB_53975| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.001
SB_17658| Best HMM Match : GAS2 (HMM E-Value=6.9e-09)                  39   0.003
SB_4896| Best HMM Match : TFIID_20kDa (HMM E-Value=2.3e-05)            36   0.026
SB_7838| Best HMM Match : Filamin (HMM E-Value=1.1e-22)                33   0.19 
SB_2620| Best HMM Match : No HMM Matches (HMM E-Value=.)               32   0.25 
SB_595| Best HMM Match : CH (HMM E-Value=0)                            31   0.43 
SB_58953| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.0  
SB_15403| Best HMM Match : CH (HMM E-Value=0)                          29   3.0  
SB_31112| Best HMM Match : Dynein_heavy (HMM E-Value=0)                28   5.3  
SB_29928| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.3  
SB_42674| Best HMM Match : DUF26 (HMM E-Value=1)                       28   5.3  

>SB_40413| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 603

 Score =  114 bits (274), Expect = 5e-26
 Identities = 77/183 (42%), Positives = 105/183 (57%), Gaps = 11/183 (6%)

Query: 7   VRAKIASKRNPEKEKEAQEWIEGVLGAKF--PPG--ELFEDVLKDGTVLCQLINKLKPGS 62
           V+A I SK +P KE+EA++WI+ VLG       G  +     LKDG VLC+L N+L  G 
Sbjct: 420 VKAGIDSKYDPTKEEEARKWIDTVLGESIFGENGGVDTVHQTLKDGQVLCRLANRL--GG 477

Query: 63  VPKINTTGGQFKMMENITNFQSAIKA-YGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRE 121
             KIN     FK MENI NF S I+   GV   D+FQTVDL+EK ++  V+  + A+GR 
Sbjct: 478 DLKINQQKMPFKQMENIGNFLSFIENNLGVAKNDLFQTVDLYEKSNMWNVICCIHAVGRR 537

Query: 122 TYRHAEWSGPCLGPKPADECKRDFSDEVLKAGQTVI-GLQAG--SNKGATQSGQNLGAGR 178
            Y   +   P LGPK + +  R F++  L  G+T+I   Q G  +   AT SGQ+ G  R
Sbjct: 538 AYSLGK-DVPQLGPKESTKNPRQFTERQLNEGKTIINSFQMGPAAKNVATASGQSFGRQR 596

Query: 179 KIL 181
           +I+
Sbjct: 597 QII 599


>SB_40412| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 592

 Score =  101 bits (242), Expect = 4e-22
 Identities = 60/146 (41%), Positives = 87/146 (59%), Gaps = 7/146 (4%)

Query: 13  SKRNPEKEKEAQEWIEGVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPKINT 68
           +K + +   +A  WIEG+LG +   G+   D    VLKDG VLC++ NKL  G   KIN+
Sbjct: 62  AKYDSDLAAQATAWIEGILGERVFGGKTGADDVHEVLKDGQVLCRVANKL--GGNIKINS 119

Query: 69  TGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEW 128
           +   FKMMEN   F       GVP  D+FQTVDL+EK+++  V++ + ALGR+ +   + 
Sbjct: 120 SKMAFKMMENTGKFLEFCDTIGVPKTDMFQTVDLYEKQNMPGVINGIHALGRKAHSTGK- 178

Query: 129 SGPCLGPKPADECKRDFSDEVLKAGQ 154
           +   LGPK A    R+F++E  +AGQ
Sbjct: 179 TCLALGPKEASANPREFTEEQRRAGQ 204


>SB_18673| Best HMM Match : CH (HMM E-Value=2.5e-05)
          Length = 195

 Score = 83.8 bits (198), Expect = 8e-17
 Identities = 61/152 (40%), Positives = 83/152 (54%), Gaps = 17/152 (11%)

Query: 10  KIASKRNPEKEKEAQEWIEGVLGAK-FPPGELFEDV---LKDGTVLCQLINKLKPGSVPK 65
           KI  K +P++E+EA+ WIE VLG K F   E  +DV   LKDG +L +L  KL  G+  K
Sbjct: 17  KILEKYDPQQEEEARIWIEAVLGEKVFGGAEGPDDVQKVLKDGKILARLAIKL--GAKIK 74

Query: 66  INTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRH 125
           +N     FK MENI NF S     GV   D FQT DL+   D A + S       +    
Sbjct: 75  VNEQNMPFKQMENIGNFLSHCGHLGVASGDQFQTADLY---DNANMTSCQLIKDLDI--- 128

Query: 126 AEWSGPCLGPKPADECKRDFSDEVLKAGQTVI 157
                P LGPK A+   R+F++E L+AG++++
Sbjct: 129 -----PTLGPKEAEANVREFTEEQLRAGESIL 155


>SB_7905| Best HMM Match : CH (HMM E-Value=1.3e-10)
          Length = 172

 Score = 75.4 bits (177), Expect = 3e-14
 Identities = 42/116 (36%), Positives = 63/116 (54%), Gaps = 5/116 (4%)

Query: 1   MSLERQVRAKIASKRNP---EKEKEAQEWIEGVLGAKF--PPGELFEDVLKDGTVLCQLI 55
           +S E    +K    + P   ++ K  + WIEG     F     + F++ LK G VLC+L 
Sbjct: 45  VSKENLKTSKFIMSKKPWPMDRAKVVRMWIEGKTSCNFGGDTADDFQNTLKSGVVLCKLA 104

Query: 56  NKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQV 111
           N ++PG++ KIN     F MMENI NF + ++  GV     F T+DL+E K++ QV
Sbjct: 105 NAIQPGAIKKINNAKMNFMMMENIENFCNFVQTKGVASQYQFVTIDLFEGKNMHQV 160


>SB_39070| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 539

 Score = 70.1 bits (164), Expect = 1e-12
 Identities = 37/118 (31%), Positives = 64/118 (54%), Gaps = 10/118 (8%)

Query: 4   ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFP---------PGELFEDVLKDGTVLCQL 54
           E+    ++ +K   E E  A+ W+E + G             PGE F + L+ G +LC++
Sbjct: 259 EKNNVGQVDAKYPKELEGNARRWVETMYGDTVAWGEERPNQRPGETFAEPLRSGVILCKI 318

Query: 55  INKLKPGSVPKINTTGGQFKMMENITNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQV 111
            NK+K  ++PKI  +   F M ENI+ F     +  G+  +++FQTVDL+E++++  V
Sbjct: 319 ANKIKSNAIPKIGESNKSFVMQENISKFLDFCERVLGLDRLNLFQTVDLFERQNVGMV 376



 Score = 37.5 bits (83), Expect = 0.007
 Identities = 22/59 (37%), Positives = 30/59 (50%), Gaps = 9/59 (15%)

Query: 3  LERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFP---------PGELFEDVLKDGTVLC 52
          L  +V  KI SK + E E + ++W+E +LG             PGE F D + DG VLC
Sbjct: 17 LTAEVNRKIQSKYDTELEMQCRDWLEKMLGENIEWGVETEYSRPGESFADGIFDGIVLC 75



 Score = 37.1 bits (82), Expect = 0.009
 Identities = 21/64 (32%), Positives = 39/64 (60%), Gaps = 6/64 (9%)

Query: 54  LINKLKPGSVPKIN-TTGGQF---KMMENITNFQSAIKA--YGVPDIDVFQTVDLWEKKD 107
           ++N++ PG++ KI+   GG+F   +++ENI  F  A +   +    +D+F   DL+EK +
Sbjct: 203 VMNEVFPGAISKIHGINGGRFHGFQILENIEKFLKACQGEPFNCNVVDLFSPGDLYEKNN 262

Query: 108 IAQV 111
           + QV
Sbjct: 263 VGQV 266


>SB_20280| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 263

 Score = 56.8 bits (131), Expect = 1e-08
 Identities = 43/125 (34%), Positives = 67/125 (53%), Gaps = 11/125 (8%)

Query: 5   RQVRAKIASKRNPEKEKEAQEWIE--GVLGAKF----PPGELFE--DVLKDGTVLCQLIN 56
           R   A  +S R+ E+ K A++W+   GVL A      P   LF+    L+DG +LCQ+ N
Sbjct: 7   RTASALSSSGRDVEEWKMARDWMISIGVLPAHGRVTDPNFTLFDFAQSLRDGVLLCQVAN 66

Query: 57  KLKPGSVPKINTTG--GQFKMMENITNFQSA-IKAYGVPDIDVFQTVDLWEKKDIAQVVS 113
            L PG+V  +       QF  ++NI NF +A  + + + D D+F   +L++  D  +VV+
Sbjct: 67  VLHPGAVVDVGMKPQMSQFMCLKNIRNFLTACTRLFNLADGDLFDANELYDVSDFGKVVN 126

Query: 114 TLFAL 118
           TL  L
Sbjct: 127 TLSKL 131


>SB_23839| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 267

 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 25/55 (45%), Positives = 38/55 (69%), Gaps = 2/55 (3%)

Query: 56  NKLKPGSVPKINTTGGQ--FKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDI 108
           N ++PG + K +   G+  FKMMENI  F + IK+YGV +  +F TVDL+EK+++
Sbjct: 189 NAIQPGVIKKPHLNPGKMGFKMMENIGWFVNFIKSYGVQEEYIFVTVDLYEKRNV 243


>SB_18675| Best HMM Match : NUMOD3 (HMM E-Value=8)
          Length = 89

 Score = 48.8 bits (111), Expect = 3e-06
 Identities = 30/83 (36%), Positives = 46/83 (55%), Gaps = 12/83 (14%)

Query: 74  KMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCL 133
           + MENI NF    ++ GV  +D+FQTVDL+EK+++A              R    + P L
Sbjct: 16  RKMENIGNFLLFCESLGVSKVDLFQTVDLYEKQNMAAA------------RAKGLNCPQL 63

Query: 134 GPKPADECKRDFSDEVLKAGQTV 156
           GPK A+   R F ++ L+AG+ +
Sbjct: 64  GPKEAEANPRSFDEDKLRAGKGI 86


>SB_36121| Best HMM Match : CH (HMM E-Value=0.0017)
          Length = 209

 Score = 45.2 bits (102), Expect = 3e-05
 Identities = 28/83 (33%), Positives = 42/83 (50%), Gaps = 1/83 (1%)

Query: 22  EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 81
           EA+ WIE      F   + F + L DG +LC+LI  +   SV +IN  G      +N++ 
Sbjct: 32  EARRWIERATFTPFKNDD-FRESLADGVLLCELIVNVANISVGRINKQGTAHAGRDNLSV 90

Query: 82  FQSAIKAYGVPDIDVFQTVDLWE 104
           F  A +A G+    +F+  DL E
Sbjct: 91  FFRACEALGLDKYQLFEHEDLDE 113


>SB_39072| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1011

 Score = 42.3 bits (95), Expect = 2e-04
 Identities = 22/66 (33%), Positives = 36/66 (54%), Gaps = 2/66 (3%)

Query: 62  SVPKINTTGGQFKMMENITNFQSAIKA--YGVPDIDVFQTVDLWEKKDIAQVVSTLFALG 119
           + P  N       M ENI NF +A +   +     D+FQTV L+E++++ QV+S + A  
Sbjct: 884 NTPSTNRKSTSLGMQENIANFLNACQEEPFNCNPQDLFQTVYLFERQNLGQVISGIQAFA 943

Query: 120 RETYRH 125
           R+  +H
Sbjct: 944 RKVRKH 949


>SB_28994| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 422

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 25/80 (31%), Positives = 39/80 (48%), Gaps = 6/80 (7%)

Query: 45  LKDGTVLCQLINKLKPGSVPKINTTGG------QFKMMENITNFQSAIKAYGVPDIDVFQ 98
           L DG VLC L+N    G++P I+            K M+N+  F  A K  GV    +  
Sbjct: 336 LADGVVLCHLVNSAYKGTIPSIHIPSAGVPKLTMTKCMKNVDYFLEACKKLGVDRELLCS 395

Query: 99  TVDLWEKKDIAQVVSTLFAL 118
           + D+ ++K   +V +T+ AL
Sbjct: 396 SADILQEKSPQRVCATVQAL 415


>SB_53975| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 43

 Score = 39.9 bits (89), Expect = 0.001
 Identities = 20/42 (47%), Positives = 29/42 (69%), Gaps = 1/42 (2%)

Query: 11 IASKRNPEKEKEAQEWIEGVLGAKFPPGE-LFEDVLKDGTVL 51
          +++K +P  + EA +WIE VLG K   G+ + EDVLKDG +L
Sbjct: 1  MSAKYDPVMDAEAVDWIEKVLGEKVCQGKSINEDVLKDGQIL 42


>SB_17658| Best HMM Match : GAS2 (HMM E-Value=6.9e-09)
          Length = 959

 Score = 38.7 bits (86), Expect = 0.003
 Identities = 36/130 (27%), Positives = 56/130 (43%), Gaps = 23/130 (17%)

Query: 17  PEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKP---------------G 61
           P KE  A  WI  +LG +    + F  +L  G VLC+L N ++                G
Sbjct: 27  PLKEDLAS-WISRLLGEEELNTDTFTSMLDTGVVLCRLANFIQTVGEEFFVRNPKFPRRG 85

Query: 62  SVPKINTT-------GGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVST 114
             P    T        G F   +N++NF    +   VPD+ +F+T DL   K+   V+ T
Sbjct: 86  LFPACGVTYKQRGATHGSFVARDNVSNFIRWCRELRVPDVIMFETEDLVLNKNEKTVLLT 145

Query: 115 LFALGRETYR 124
           L  + R+ ++
Sbjct: 146 LLEVARKAFK 155


>SB_4896| Best HMM Match : TFIID_20kDa (HMM E-Value=2.3e-05)
          Length = 819

 Score = 35.5 bits (78), Expect = 0.026
 Identities = 17/36 (47%), Positives = 22/36 (61%)

Query: 76  MENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQV 111
           M  I  F      +GV   D+FQTVDL+EK++I QV
Sbjct: 700 MVTIAKFLDFCGTFGVAKSDLFQTVDLYEKQNIQQV 735



 Score = 27.9 bits (59), Expect = 5.3
 Identities = 11/27 (40%), Positives = 19/27 (70%)

Query: 6   QVRAKIASKRNPEKEKEAQEWIEGVLG 32
           ++  K+++K +P  + EA +WIE VLG
Sbjct: 671 ELSRKMSAKYDPVMDAEAVDWIEKVLG 697


>SB_7838| Best HMM Match : Filamin (HMM E-Value=1.1e-22)
          Length = 820

 Score = 32.7 bits (71), Expect = 0.19
 Identities = 15/52 (28%), Positives = 25/52 (48%)

Query: 41  FEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVP 92
           F+   +DG  +C+L+N L+PG +P                  Q+A  A+G+P
Sbjct: 449 FDTDWRDGMAICELVNALQPGLIPDSKYKEKTNSEATAKIGIQTAHDAFGIP 500


>SB_2620| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 37

 Score = 32.3 bits (70), Expect = 0.25
 Identities = 12/27 (44%), Positives = 21/27 (77%)

Query: 131 PCLGPKPADECKRDFSDEVLKAGQTVI 157
           P LGPK A+   R+F++E L+AG++++
Sbjct: 10  PTLGPKEAEANVREFTEEQLRAGESIL 36


>SB_595| Best HMM Match : CH (HMM E-Value=0)
          Length = 905

 Score = 31.5 bits (68), Expect = 0.43
 Identities = 27/88 (30%), Positives = 40/88 (45%), Gaps = 3/88 (3%)

Query: 19  KEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMM-E 77
           +EK  + WI   LG K     L+ D L DG +L QL ++++PG V         FK M  
Sbjct: 674 EEKTFRNWINS-LGIKPFVQNLYLD-LDDGMILFQLFDQVQPGIVNYDKVNKPPFKKMGA 731

Query: 78  NITNFQSAIKAYGVPDIDVFQTVDLWEK 105
            +   ++   A  V D+  F  V +  K
Sbjct: 732 KMKKLENCNYAISVADLLKFSLVGVGGK 759


>SB_58953| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 377

 Score = 28.7 bits (61), Expect = 3.0
 Identities = 13/26 (50%), Positives = 17/26 (65%)

Query: 133 LGPKPADECKRDFSDEVLKAGQTVIG 158
           LGP P+D+  RDF    L++GQ V G
Sbjct: 233 LGPDPSDKKVRDFIWNTLQSGQVVPG 258


>SB_15403| Best HMM Match : CH (HMM E-Value=0)
          Length = 1907

 Score = 28.7 bits (61), Expect = 3.0
 Identities = 12/25 (48%), Positives = 19/25 (76%), Gaps = 1/25 (4%)

Query: 39  ELFEDVLKDGTVLCQLINKLKPGSV 63
           +L+E V KDG +LC++IN   PG++
Sbjct: 78  DLYEKV-KDGILLCKMINLSAPGTI 101


>SB_31112| Best HMM Match : Dynein_heavy (HMM E-Value=0)
          Length = 2532

 Score = 27.9 bits (59), Expect = 5.3
 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%)

Query: 26   WIEGVLGAKF--PPGELFEDVLKDGTVLCQLINKLKPGSVP 64
            ++ G +G K+  PP   FE +L+  T    +I  L PGS P
Sbjct: 1904 FVTGRMGEKYVTPPVLSFESILEQSTPFSPIIFILSPGSDP 1944


>SB_29928| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 297

 Score = 27.9 bits (59), Expect = 5.3
 Identities = 13/43 (30%), Positives = 21/43 (48%)

Query: 28  EGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTG 70
           +G LGA F P +LF   + +  +   ++N    G V +   TG
Sbjct: 73  QGSLGANFDPSKLFYGYMANLNIWANVLNDFNRGKVYEQGCTG 115


>SB_42674| Best HMM Match : DUF26 (HMM E-Value=1)
          Length = 493

 Score = 27.9 bits (59), Expect = 5.3
 Identities = 24/86 (27%), Positives = 36/86 (41%), Gaps = 4/86 (4%)

Query: 31  LGAKFPPGELFEDV--LKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQSAIKA 88
           L A  PP     DV  L  GT    L   LKP   PK+    G  + + +I  F S++ A
Sbjct: 89  LMATLPPTSTAHDVERLSTGTKFA-LRQWLKPIVAPKVCNNTGLHRPVAHIPPFTSSVAA 147

Query: 89  YGVPDIDVFQTVDLWEKKDIAQVVST 114
            G   I     +  W   D ++++ +
Sbjct: 148 QGKAPIKSVSALS-WPGTDQSEIMDS 172


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.315    0.134    0.394 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,319,180
Number of Sequences: 59808
Number of extensions: 250668
Number of successful extensions: 374
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 337
Number of HSP's gapped (non-prelim): 28
length of query: 184
length of database: 16,821,457
effective HSP length: 78
effective length of query: 106
effective length of database: 12,156,433
effective search space: 1288581898
effective search space used: 1288581898
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 57 (27.1 bits)

- SilkBase 1999-2023 -