Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001201-TA|BGIBMGA001201-PA|IPR003247|Calponin-like
actin-binding subtype, IPR001715|Calponin-like actin-binding,
IPR000557|Calponin repeat, IPR003096|SM22/calponin
(184 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09170.1 68414.m01024 kinesin motor protein-related similar t... 50 1e-06
At2g47500.1 68415.m05929 kinesin motor protein-related 48 4e-06
At5g27000.1 68418.m03221 kinesin motor protein-related non-conse... 47 7e-06
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 44 8e-05
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina... 44 8e-05
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina... 44 8e-05
At5g41310.1 68418.m05020 kinesin motor protein-related 39 0.002
At5g35700.1 68418.m04269 fimbrin-like protein, putative similar ... 39 0.002
At5g48460.1 68418.m05992 fimbrin-like protein, putative strong s... 35 0.038
At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 34 0.066
At2g04750.1 68415.m00485 fimbrin-like protein, putative similar ... 34 0.066
At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical t... 33 0.12
At5g55400.1 68418.m06902 fimbrin-like protein, putative similar ... 33 0.15
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 30 1.1
At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-... 29 1.9
At5g02780.1 68418.m00220 In2-1 protein, putative similar to In2-... 29 1.9
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 29 2.5
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 28 4.3
At5g14570.1 68418.m01708 transporter, putative similar to trans-... 28 4.3
At4g18200.1 68417.m02705 purine permease family protein similar ... 28 4.3
At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family prote... 27 5.7
>At1g09170.1 68414.m01024 kinesin motor protein-related similar to
GB:AAB61066
Length = 1010
Score = 49.6 bits (113), Expect = 1e-06
Identities = 38/128 (29%), Positives = 64/128 (50%), Gaps = 24/128 (18%)
Query: 22 EAQEWIE---GVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPK--------- 65
EA W+ GV+G + P + E+ L+ G +LC ++N++KPG+VPK
Sbjct: 59 EAARWVRNTLGVVGGRDLPADPSEEDFRIALRSGILLCNVLNRVKPGAVPKVVEAPNDPL 118
Query: 66 INTTG---GQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRET 122
+N G F+ EN+ NF ++ G+P F+ D + A++V + AL ++
Sbjct: 119 VNQDGAALSAFQYFENLRNFLVFVEEMGIP---TFEVSDFEKGGKSARIVECVLAL--KS 173
Query: 123 YRHAEWSG 130
YR + SG
Sbjct: 174 YREWKQSG 181
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 48.0 bits (109), Expect = 4e-06
Identities = 36/116 (31%), Positives = 59/116 (50%), Gaps = 22/116 (18%)
Query: 22 EAQEWIE---GVLGAKFPPGELFEDVLK----DGTVLCQLINKLKPGSVPKI-------- 66
EA W+ GV+GAK P E E+ L+ G +LC+++NK++PG+V K+
Sbjct: 49 EAANWLRRMVGVVGAKDLPAEPTEEGLRLGLRSGIILCKVLNKVQPGAVSKVVESPCDAI 108
Query: 67 ----NTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118
F+ EN+ NF AI+ G P F+ DL + + ++VV+ + A+
Sbjct: 109 LVADGAPLSAFQYFENVRNFLVAIQEMGFP---TFEASDLEQGGNASRVVNCVLAI 161
>At5g27000.1 68418.m03221 kinesin motor protein-related
non-consensus AT donor splice site at exon 12;
non-consensus AC acceptor splice site at exon 13
Length = 987
Score = 47.2 bits (107), Expect = 7e-06
Identities = 37/113 (32%), Positives = 57/113 (50%), Gaps = 19/113 (16%)
Query: 22 EAQEWIEGVLGA---KFPPGELFEDV----LKDGTVLCQLINKLKPGSVPKI-----NTT 69
EA W+ ++G K PGE E+ L+ G VLC ++NK+ PGSV K+ +
Sbjct: 49 EAAGWLRDMIGVSNGKDFPGEPSEEEFRLGLRSGIVLCNVLNKVNPGSVSKVVEAPDDVA 108
Query: 70 GG----QFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118
G F+ ENI NF AI+ G+P F+ D+ + ++V+ + AL
Sbjct: 109 DGAALSAFQYFENIRNFLVAIEEMGLPS---FEASDMEKGGKSIRIVNCILAL 158
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 43.6 bits (98), Expect = 8e-05
Identities = 33/126 (26%), Positives = 61/126 (48%), Gaps = 18/126 (14%)
Query: 11 IASKRNPE---KEKEAQEWIEGVLGA----KFPPGELFEDVLKDGTVLCQLINKLKPGSV 63
+AS+R E + +A +W++ V+G P + F L++G +LC INK+ PG+V
Sbjct: 10 LASRRAEEAAARRFQAVQWLKSVVGQLGIPNQPSEKEFISCLRNGMILCNAINKIHPGAV 69
Query: 64 PKI----NTTGGQFKM------MENITNFQSAIKAYGVPDIDVFQ-TVDLWEKKDIAQVV 112
K+ + G++++ EN+ NF A++ +P + D E + +VV
Sbjct: 70 SKVVENYSYLNGEYQLPPAYQYFENVRNFLVALETLRLPGFEASDLEKDNLESGSVTKVV 129
Query: 113 STLFAL 118
+ L
Sbjct: 130 DCILGL 135
>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1065
Score = 43.6 bits (98), Expect = 8e-05
Identities = 35/99 (35%), Positives = 50/99 (50%), Gaps = 13/99 (13%)
Query: 25 EWIEGVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPKINTTGGQFKMME-NI 79
EW+ L P E ED L+DGTVLC L+N+L PGS+ GG F+ I
Sbjct: 47 EWLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSM----RMGGSFEPASVKI 102
Query: 80 TNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118
F +A+ +P F+ D+ E+ D+ V+ +L AL
Sbjct: 103 ERFLTAMDEMALPR---FEVSDI-EQGDMVPVLQSLKAL 137
>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1064
Score = 43.6 bits (98), Expect = 8e-05
Identities = 35/99 (35%), Positives = 50/99 (50%), Gaps = 13/99 (13%)
Query: 25 EWIEGVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPKINTTGGQFKMME-NI 79
EW+ L P E ED L+DGTVLC L+N+L PGS+ GG F+ I
Sbjct: 47 EWLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSM----RMGGSFEPASVKI 102
Query: 80 TNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118
F +A+ +P F+ D+ E+ D+ V+ +L AL
Sbjct: 103 ERFLTAMDEMALPR---FEVSDI-EQGDMVPVLQSLKAL 137
>At5g41310.1 68418.m05020 kinesin motor protein-related
Length = 961
Score = 39.1 bits (87), Expect = 0.002
Identities = 28/82 (34%), Positives = 40/82 (48%), Gaps = 9/82 (10%)
Query: 16 NPEKEKEAQEWIEGVLGAKFPPGELFEDVLK----DGTVLCQLINKLKPGSVPKINTTGG 71
N + + EW+ L P E E+ L+ DGTVLC L+N+L PGS+ GG
Sbjct: 39 NKQGHQSLVEWLNETLPYLNLPWEASEEELRACLVDGTVLCNLLNQLSPGSM----RMGG 94
Query: 72 QFKM-MENITNFQSAIKAYGVP 92
F+ NI F +A+ +P
Sbjct: 95 SFEPGCVNIERFLAAMDEMTLP 116
>At5g35700.1 68418.m04269 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893, fimbrin [Schizosaccharomyces pombe]
GI:3057144; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 687
Score = 39.1 bits (87), Expect = 0.002
Identities = 23/65 (35%), Positives = 38/65 (58%), Gaps = 4/65 (6%)
Query: 36 PPGELFEDVLKDGTVLCQLINKLKPGSVPK--INT--TGGQFKMMENITNFQSAIKAYGV 91
P F D++KDG +LC+LIN PG++ + INT T ++ EN+T ++ KA G
Sbjct: 150 PATNAFFDLVKDGVLLCKLINVAVPGTIDERAINTKKTLNPWERNENLTLGLNSAKAIGC 209
Query: 92 PDIDV 96
+++
Sbjct: 210 TVVNI 214
>At5g48460.1 68418.m05992 fimbrin-like protein, putative strong
similarity to fimbrin-like protein AtFim2 [Arabidopsis
thaliana] GI:2737926; contains Pfam profile PF00307:
Calponin homology (CH) domain
Length = 654
Score = 34.7 bits (76), Expect = 0.038
Identities = 23/62 (37%), Positives = 36/62 (58%), Gaps = 5/62 (8%)
Query: 39 ELFEDVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDI 94
+LFE V KDG +LC+LIN PG++ + INT ++ EN T ++ KA G +
Sbjct: 156 DLFE-VAKDGVLLCKLINVAVPGTIDERAINTKSMLNPWERNENHTLCLNSAKAIGCTVV 214
Query: 95 DV 96
++
Sbjct: 215 NI 216
Score = 31.1 bits (67), Expect = 0.47
Identities = 17/45 (37%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
Query: 19 KEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSV 63
+EK + WI G+ + +FED L+DG +L Q ++K+ PG V
Sbjct: 396 EEKAFRFWINSFDGSVYI-NNVFED-LRDGWILLQTLDKVSPGIV 438
>At3g44730.1 68416.m04814 kinesin motor protein-related similar to
4 other kinesin-like proteins of A. thaliana: F02P16.12
(PID:g2191180), katA (D11371), katB (D21137), and katC
(D21138); contains non-consensus AT-AC splice sites at
intron 10
Length = 1087
Score = 33.9 bits (74), Expect = 0.066
Identities = 13/31 (41%), Positives = 21/31 (67%)
Query: 36 PPGELFEDVLKDGTVLCQLINKLKPGSVPKI 66
P + F L++G +LC ++NK+ PGSV K+
Sbjct: 13 PSEDEFSLALRNGLILCNVLNKVNPGSVLKV 43
>At2g04750.1 68415.m00485 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 652
Score = 33.9 bits (74), Expect = 0.066
Identities = 19/58 (32%), Positives = 35/58 (60%), Gaps = 4/58 (6%)
Query: 43 DVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDIDV 96
D++KDG +LC+LIN PG++ + INT ++ EN++ ++ KA G +++
Sbjct: 151 DLVKDGVLLCKLINIAVPGTIDERAINTKKELNPWERTENLSLCLNSAKAIGCTVVNI 208
>At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893
Length = 687
Score = 33.1 bits (72), Expect = 0.12
Identities = 25/70 (35%), Positives = 41/70 (58%), Gaps = 6/70 (8%)
Query: 39 ELFEDVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDI 94
+L+E ++KDG +LC+LIN PG++ + INT ++ EN T ++ KA G +
Sbjct: 155 QLYE-LVKDGVLLCKLINVAVPGTIDERAINTKRVLNPWERNENHTLCLNSAKAVGCSVV 213
Query: 95 DVFQTVDLWE 104
++ T DL E
Sbjct: 214 NI-GTQDLAE 222
>At5g55400.1 68418.m06902 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 714
Score = 32.7 bits (71), Expect = 0.15
Identities = 25/70 (35%), Positives = 41/70 (58%), Gaps = 6/70 (8%)
Query: 39 ELFEDVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDI 94
+L+E ++KDG +LC+LIN PG++ + INT ++ EN T ++ KA G +
Sbjct: 156 DLYE-LVKDGVLLCKLINIAVPGTIDERAINTKRVLNPWERNENHTLCLNSAKAVGCSVV 214
Query: 95 DVFQTVDLWE 104
++ T DL E
Sbjct: 215 NI-GTQDLAE 223
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 29.9 bits (64), Expect = 1.1
Identities = 35/117 (29%), Positives = 54/117 (46%), Gaps = 15/117 (12%)
Query: 7 VRAKIASKRNPEKEK---EAQEWIEGVLGAKF-----PPGELFEDVLKD--GTVLCQL-I 55
+R+ +ASKR E+ K EA EW+ G + ++F PGE+ V G Q+ +
Sbjct: 1043 LRSTLASKRVLEEYKLSREAFEWVIGEIESRFLQSLVAPGEMIGCVAAQSIGEPATQMTL 1102
Query: 56 NKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVV 112
N V N T G ++ E I N IK P + V+ T + + K+ A+ V
Sbjct: 1103 NTFHYAGVSAKNVTLGVPRLRE-IINVAKRIK---TPSLSVYLTPEASKSKEGAKTV 1155
>At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-1,
Zea mays, EMBL:X58573
Length = 235
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/44 (38%), Positives = 22/44 (50%), Gaps = 2/44 (4%)
Query: 123 YRHAEWSGPCLGPKPADECKRDFSDEVLKAGQTVIGLQAGSNKG 166
Y + GP L P+ D KR+F DE+LK T + S KG
Sbjct: 99 YLDNTFEGPSLYPE--DHAKREFGDELLKYTDTFVKTMYVSLKG 140
>At5g02780.1 68418.m00220 In2-1 protein, putative similar to In2-1
[Zea mays] EMBL:X58573
Length = 237
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/45 (37%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Query: 123 YRHAEWSGPCLGPKPADECKRDFSDEVLK-AGQTVIGLQAGSNKG 166
Y + + GP L P+ D KR+F +E+LK +T + GS KG
Sbjct: 101 YVDSNFDGPSLYPE--DSAKREFGEELLKYVDETFVKTVFGSFKG 143
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 28.7 bits (61), Expect = 2.5
Identities = 19/67 (28%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Query: 10 KIASKRNPEKEKEAQEWIEGVL----GAKFPPGELFEDVLKDGTVLCQ-LINKLKPGSVP 64
KIASK + +K+ ++ I+ + G + + FED +K+ LC +I ++ G+ P
Sbjct: 563 KIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQGAGP 622
Query: 65 KINTTGG 71
+ GG
Sbjct: 623 DMGGAGG 629
>At5g67270.1 68418.m08480 microtubule-associated EB1 family
protein similar to SP|Q9UPY8 Microtubule-associated
protein RP/EB family member 3 (Protein EB3) {Homo
sapiens}; contains Pfam profiles PF00307: Calponin
homology (CH) domain, PF03271: EB1 protein
Length = 329
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 3/34 (8%)
Query: 48 GTVLCQLINKLKPGSVP--KIN-TTGGQFKMMEN 78
G V CQL++ + PG+VP K+N +++M++N
Sbjct: 40 GAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQN 73
>At5g14570.1 68418.m01708 transporter, putative similar to
trans-membrane nitrate transporter protein AtNRT2:1
[Arabidopsis thaliana] GI:3747058, high-affinity nitrate
transporter ACH1 [Arabidopsis thaliana] GI:3608362;
contains Pfam profile PF00083: Sugar (and other)
transporter
Length = 493
Score = 27.9 bits (59), Expect = 4.3
Identities = 26/104 (25%), Positives = 48/104 (46%), Gaps = 6/104 (5%)
Query: 49 TVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDI 108
+V Q + L G VP ++T ++ IT + A V +F D+ +++ I
Sbjct: 375 SVFVQAASGLVFGVVPFVSTRS--LGVVAGITGSGGTVGAV-VTQFLLFSGDDVRKQRSI 431
Query: 109 AQV--VSTLFALGRETYRHAEWSGPCLGPKPADECKRDFSDEVL 150
+ + ++ +FAL + +W G C GP + E + D S +L
Sbjct: 432 SLMGLMTFVFALSVTSIYFPQWGGMCCGPSSSSE-EEDISRGLL 474
>At4g18200.1 68417.m02705 purine permease family protein similar to
purine permease [Arabidopsis thaliana] GI:7620007;
contains Pfam profile PF03151: Domain of unknown
function, DUF250
Length = 1128
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/54 (24%), Positives = 29/54 (53%)
Query: 65 KINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 118
++ T G + E+ + F ++I A G+P + V + +K D +++ S + A+
Sbjct: 1043 QVYTVGCVGLIFESSSVFSNSITAVGLPIVPVVAVIVFHDKMDASKIFSIILAI 1096
>At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family
protein low similarity to hydrolases from Rhodococcus
sp. EtbD2 GI:3273241, EtbD1 GI:3273239; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 314
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/23 (56%), Positives = 16/23 (69%)
Query: 45 LKDGTVLCQLINKLKPGSVPKIN 67
LKDGTV+ ++K KP S PK N
Sbjct: 31 LKDGTVVNFWVSKTKPESKPKPN 53
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.134 0.394
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,635,049
Number of Sequences: 28952
Number of extensions: 192423
Number of successful extensions: 333
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 312
Number of HSP's gapped (non-prelim): 23
length of query: 184
length of database: 12,070,560
effective HSP length: 77
effective length of query: 107
effective length of database: 9,841,256
effective search space: 1053014392
effective search space used: 1053014392
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 57 (27.1 bits)
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