GeneModel BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001197-TA|BGIBMGA001197-PA|IPR003307|eIF4-
gamma/eIF5/eIF2-epsilon
         (535 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon...   162   1e-40
SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc...    39   0.002
SPAC4D7.09 |tif223||translation initiation factor eIF2B|Schizosa...    31   0.30 
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual        30   0.93 
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb...    28   2.8  
SPAC2F7.05c |||translation initiation factor eIF5 |Schizosacchar...    27   5.0  
SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos...    27   8.6  

>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 678

 Score =  162 bits (393), Expect = 1e-40
 Identities = 86/275 (31%), Positives = 160/275 (58%), Gaps = 7/275 (2%)

Query: 6   ENIVQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAILFCCQNG 65
           ++ +QA+V+ D++N  F PLT +KP   L +A  PLI+Y  E LAL GV E  +FCC + 
Sbjct: 15  KHALQAIVLSDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAGVQEVYVFCCAHA 74

Query: 66  QKIKEHVQKHQDNKSLWSLTMDIQILMSDTCQTMGDVMRELDAAALLKGYFVLAGINSIT 125
            +I+E+++K + N  L S    +  ++S    ++GD +RELD+  L+   F+L   + ++
Sbjct: 75  GQIREYIEKSKWN--LPSSPFSVNTIVSRESLSVGDALRELDSKQLITSDFILVSGDVVS 132

Query: 126 NMNFASLLEQHKQTCKKDKGTAMTLVYKKLSWEHPLISSDRSTFLAANENTKKVLVHKRY 185
           N+    +L++H++  + DK   MT+V ++ S  H   +   S+    ++ T +  VH + 
Sbjct: 133 NVPLNEVLKEHRKRREDDKNAIMTMVVREASPFHRTRARTESSVFVIDKKTSQ-CVHYQA 191

Query: 186 RPKSKEKIISLPLDLVLNNSEVKLHHNLVDTNIALCSPTVPPLFSDNFDFQ-TRDDFIHG 244
             + K   +S+  ++   + E+++ ++L+D  I +CS  VP LF++NFD+Q  R DF++G
Sbjct: 192 NERGKH-YVSMDPEIFNEHEELEVRNDLIDCQIDICSNDVPALFTENFDYQDIRKDFVYG 250

Query: 245 ILINEEILASSLYYTLLKSNQYAAAITNWKTYQTV 279
           +L   ++L   ++  + K N YAA + + +TY  +
Sbjct: 251 VL-TSDLLGKKIHCHVAKEN-YAARVRSLQTYDAI 283



 Score = 59.7 bits (138), Expect = 1e-09
 Identities = 38/164 (23%), Positives = 78/164 (47%), Gaps = 4/164 (2%)

Query: 374 FLQEVIDSLARGYDEKLKCDYLILEINSSRYAYNIQLNEVNFFVVRALLSMPVLSEAKNV 433
           F +E   SL R ++E  + D   LE+N+ R A N   +EV   +V ALL   ++    + 
Sbjct: 514 FNKEAQQSLERAFEENHQIDIAALELNTLRMAMNANYHEVRSAIVLALLRR-IMHLDVSP 572

Query: 434 LTTVKDILKYFRPVLANYIKSKSSIMDCLRAVEDTCLNCEWLDGKSGQILHLLYEADVVD 493
              +  ++  + P+LA    S    +D +  ++  C+    +     Q+L   Y+ ++ +
Sbjct: 573 KEALAKVMTRWGPLLAKLTFSHEEQVDNVLTLQKYCVRLS-MTRHFLQLLGYFYQLEIAE 631

Query: 494 EDSLVDWYAELKENANPF--VKQPSLVKFFEWLQEASEESDDSE 535
           E+++ +WY++ + +      ++     +F +WL  A  ES+  E
Sbjct: 632 ENAIQEWYSDPRSSEGELAALRDAGGKQFVDWLNTAESESESEE 675


>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
           Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 363

 Score = 38.7 bits (86), Expect = 0.002
 Identities = 29/129 (22%), Positives = 60/129 (46%), Gaps = 4/129 (3%)

Query: 9   VQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAILFCCQNGQKI 68
           ++A++++  F +   PLT   P   +E    P+I + +E+LA  GV + +L      + +
Sbjct: 1   MKALILVGGFGTRLRPLTLTLPKPLVEFGNKPMILHQVEALAAAGVTDIVLAVNYRPEIM 60

Query: 69  KEHVQKHQDNKSLWSLTMDIQILMSDTCQTMGDVMRELDAAALLKGYFVLAGINSITNMN 128
            E ++K++   ++ ++T  ++   ++   T G +    D  A     F +   + I    
Sbjct: 61  VEALKKYEKEYNV-NITFSVE---NEPLGTAGPLALARDILAKDHSPFFVLNSDVICEYP 116

Query: 129 FASLLEQHK 137
           FA L   HK
Sbjct: 117 FADLAAFHK 125


>SPAC4D7.09 |tif223||translation initiation factor
          eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 468

 Score = 31.5 bits (68), Expect = 0.30
 Identities = 20/59 (33%), Positives = 27/59 (45%), Gaps = 3/59 (5%)

Query: 10 QAVVIMDTFNSNFSPLT--DNKPMGFLEVAGVPLIDYVLESLALGGVGEAILFCCQNGQ 66
          QAVV    F ++  PLT  D  P   L +   P++ Y L  L   G   AIL C +  +
Sbjct: 40 QAVVFAG-FGNSLYPLTGSDALPKALLPIGNKPMLHYPLYWLEAAGFTSAILICMEEAE 97


>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 510

 Score = 29.9 bits (64), Expect = 0.93
 Identities = 20/95 (21%), Positives = 48/95 (50%), Gaps = 5/95 (5%)

Query: 375 LQEVIDSLARGYDEKLKCDYLILEINSSRYAYN--IQLNEVNFFVVRALLSMPVLSEAKN 432
           L + ++SL + Y  K+    ++ E N   Y     + LN++   V+    +  ++    N
Sbjct: 251 LADAVESL-KVYQTKIA--RILREENEDEYYVQKLLSLNDLLINVIEECSNSDLIHSGTN 307

Query: 433 VLTTVKDILKYFRPVLANYIKSKSSIMDCLRAVED 467
           V+++  ++++   P  +N  K +SS++D ++  E+
Sbjct: 308 VVSSQPNVVESHVPPSSNDTKQESSLIDLMKLTEE 342


>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pombe|chr
            3|||Manual
          Length = 1254

 Score = 28.3 bits (60), Expect = 2.8
 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)

Query: 141  KKDKGTAMTLVYKKLSWEHPLISSDRSTFLAANENTKKVL 180
            K+   T MT +Y +L     ++S+ RSTF+     TKK+L
Sbjct: 1053 KRASITPMTSIYTRLGANDDIMSA-RSTFMVELSETKKIL 1091


>SPAC2F7.05c |||translation initiation factor eIF5
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 395

 Score = 27.5 bits (58), Expect = 5.0
 Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 4/59 (6%)

Query: 481 QILHLLYEADVVDEDSLVDWYAELKEN--ANPFVKQ--PSLVKFFEWLQEASEESDDSE 535
           ++L  +Y+ D+V +D    W A+  +   +    K+   +   F  WL EAS+ES+  +
Sbjct: 327 KVLLEIYQNDLVSDDMFEKWGAKASKKYVSRETSKKIHEAAEPFLTWLAEASDESESED 385


>SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 790

 Score = 26.6 bits (56), Expect = 8.6
 Identities = 14/43 (32%), Positives = 19/43 (44%)

Query: 493 DEDSLVDWYAELKENANPFVKQPSLVKFFEWLQEASEESDDSE 535
           D + +V  Y +  +N NP +   SL K F       E  DD E
Sbjct: 365 DSEKVVQVYTDAIKNVNPKLAHGSLGKLFSEFARFYENFDDLE 407


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.390 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,297,573
Number of Sequences: 5004
Number of extensions: 94291
Number of successful extensions: 246
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 236
Number of HSP's gapped (non-prelim): 8
length of query: 535
length of database: 2,362,478
effective HSP length: 76
effective length of query: 459
effective length of database: 1,982,174
effective search space: 909817866
effective search space used: 909817866
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 56 (26.6 bits)