Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001197-TA|BGIBMGA001197-PA|IPR003307|eIF4-
gamma/eIF5/eIF2-epsilon
(535 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18300.1 68417.m02715 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 134 1e-31
At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 134 1e-31
At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 103 2e-22
At1g36730.1 68414.m04569 eukaryotic translation initiation facto... 46 5e-05
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 44 3e-04
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 43 4e-04
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 41 0.002
At3g55590.1 68416.m06173 GDP-mannose pyrophosphorylase, putative... 33 0.63
At2g39770.1 68415.m04883 GDP-mannose pyrophosphorylase (GMP1) i... 33 0.63
At3g17340.1 68416.m02216 importin-related contains Pfam profile ... 30 3.4
At1g24330.1 68414.m03069 armadillo/beta-catenin repeat family pr... 30 4.4
At1g77680.1 68414.m09044 ribonuclease II family protein weak sim... 29 7.7
At1g75140.1 68414.m08728 expressed protein 29 7.7
>At4g18300.1 68417.m02715 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q64350
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 709
Score = 134 bits (324), Expect = 1e-31
Identities = 85/276 (30%), Positives = 157/276 (56%), Gaps = 15/276 (5%)
Query: 9 VQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAILFCCQNGQKI 68
+QA+++ D+F + F +T +P L + +P+IDY L L G+ E +FCC + +I
Sbjct: 24 LQAILLADSFTTQFRSITLERPKVLLPIVNIPMIDYTLAWLESAGIEEVFVFCCAHSSQI 83
Query: 69 KEHVQKHQDNKSLWSLTMDIQILMSDTCQTMGDVMR----ELDAAALLKGYFVLAGINSI 124
++++K + + + + ++ ++S ++GD +R + + ++G FVL +++
Sbjct: 84 IDYLKK-SEWYTRPNPNLLVRTIVSHNSTSVGDALRYIYEQQTETSQIQGDFVLVSGDTV 142
Query: 125 TNMNFASLLEQHKQTCKKDKGTAMTLVYKKLSWEHPLISSDRSTFLAANENTKKVLVHKR 184
+NM A L++QH+ KKD+ MT+V+KK S I SD+ F+A + TK++L ++
Sbjct: 143 SNMPLADLIQQHRDRKKKDEKAIMTMVFKKQS--RLGIGSDQ-LFVAVDPLTKQLLHYEE 199
Query: 185 YRPKSKEKIISLPLDLVLNNSEVKLHHNLVDTNIALCSPTVPPLFSDNFDFQ-TRDDFIH 243
+ + LD L +S V L ++ D I +CSP V LF DNFD+Q R F++
Sbjct: 200 CNSRGGD----FCLDKSLLDSTV-LCSDMQDCYIDICSPEVLSLFEDNFDYQHMRRHFVN 254
Query: 244 GILINEEILASSLYYTLLKSNQYAAAITNWKTYQTV 279
G+L+ ++I+ ++ ++S+ YAA I N ++Y TV
Sbjct: 255 GLLV-DDIMGYKIFTHEIQSSCYAARIDNLRSYDTV 289
Score = 66.5 bits (155), Expect = 4e-11
Identities = 46/165 (27%), Positives = 79/165 (47%), Gaps = 6/165 (3%)
Query: 374 FLQEVIDSLARGYDEKLKCDYLILEINSSRYAYNIQLNEVNFFVVRALLSMPVLS---EA 430
F +EV + ++ K D +I INS R AYN+ + V +++ + V S A
Sbjct: 544 FEREVEEEFLSAVEKDNKADIVIPLINSHRLAYNMDSADCAGAVFYSMMRLAVKSPHNSA 603
Query: 431 KNVLTTVKDILKYFRPVLANYIKSKSSIMDCLRAVEDTCLNCEWLDGKSGQILHLLYEAD 490
+ ++ ++ VL Y+K + ++ + E+ C E L ++L LY+ D
Sbjct: 604 TELYRNAMGVITKWKGVLGFYLKQTDAQIEVIMKFEEMCQESEELSPLFAKLLPFLYDKD 663
Query: 491 VVDEDSLVDWYAELKENANPF--VKQPSLVKFFEWLQEASEESDD 533
VV ED+++ W E K A+ V F +WL+EASEE ++
Sbjct: 664 VVQEDAILRW-GEEKAGADDCDKVYLNKCESFIKWLKEASEEEEE 707
>At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q64350
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 730
Score = 134 bits (324), Expect = 1e-31
Identities = 85/287 (29%), Positives = 153/287 (53%), Gaps = 15/287 (5%)
Query: 2 EMDKENIVQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAILFC 61
E+ + +QA+++ D+F + F P+T +P L + VP+IDY L L G+ E +FC
Sbjct: 18 EVQSRHRLQAILLADSFATKFRPVTLERPKVLLPIVNVPMIDYTLAWLESAGIEEVFVFC 77
Query: 62 CQNGQKIKEHVQKHQDNKSLWSLTMDIQILMSDTCQTMGDVMR----ELDAAALLKGYFV 117
C + ++ E+++K + L I+ S + GD +R + + ++G FV
Sbjct: 78 CAHSMQVIEYLEKSEWYSHPNLLVRTIE---SHKSISAGDALRYMYEQQTETSQIQGDFV 134
Query: 118 LAGINSITNMNFASLLEQHKQTCKKDKGTAMTLVYKKLSWEHPLISSDR----STFLAAN 173
L ++++NM A L+++H++ KKD+ MT+V K+ S PL R F+A +
Sbjct: 135 LVSGDTVSNMPLADLIQEHRERKKKDEKAIMTMVIKQ-SKSSPLTHQSRLGTDQLFIAVD 193
Query: 174 ENTKKVLVHKRYRPKSKEKIISLPLDLVLNNSEVKLHHNLVDTNIALCSPTVPPLFSDNF 233
TK++L ++ + + L L+ N V + +++ D I +CSP V LF DNF
Sbjct: 194 PLTKQLLHYEEDKIDHPSGSVCLEKSLLDTNPSVLVCNDMQDCYIDICSPEVLSLFEDNF 253
Query: 234 DFQ-TRDDFIHGILINEEILASSLYYTLLKSNQYAAAITNWKTYQTV 279
D+Q R F+ G+L+ ++I+ ++ + S+ YA I N+++Y TV
Sbjct: 254 DYQHLRRHFVKGVLV-DDIMGYKIFTHEIHSS-YAGRIDNFRSYDTV 298
Score = 70.1 bits (164), Expect = 3e-12
Identities = 49/170 (28%), Positives = 87/170 (51%), Gaps = 11/170 (6%)
Query: 374 FLQEVIDSLARGYDEKLKCDYLILEINSSRYAYNIQLNE---VNFF-VVRALLSMPVLSE 429
F +EV ++ R +E +K D + +EIN R ++N++ + FF +++ L P S
Sbjct: 555 FEKEVEGTVLRAVEENIKVDLVTMEINGLRLSFNMESADCAGATFFSMIKLALDTPHNSG 614
Query: 430 AKNVLTTVKDILKYFRPVLANYIKSKSSIMDCLRAVEDTCLNC-EWLDGKSGQILHLLYE 488
++ + I+ ++ +L Y K ++ + E+ C + L QILHLLY+
Sbjct: 615 SE-LYKNAASIITKWKDLLGFYAKKIDEQIEVIMKFEEMCQESHKELGPLFTQILHLLYD 673
Query: 489 ADVVDEDSLVDWYAE---LKENANPFVKQPSLVKFFEWLQEASEESDDSE 535
DV+ ED+++ W E E ++KQ F +WL+EASEE D+ +
Sbjct: 674 KDVLQEDAILRWEEEKAGADEADKVYLKQCD--TFIQWLKEASEEEDEDD 721
>At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 676
Score = 103 bits (248), Expect = 2e-22
Identities = 81/286 (28%), Positives = 141/286 (49%), Gaps = 31/286 (10%)
Query: 2 EMDKENIVQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAILFC 61
E + +QA+++ D+F + PLT +P L + +P+IDY L L G+ E +FC
Sbjct: 18 EQSRRQRLQAILLADSFATKLLPLTLERPNVLLPLVNIPMIDYTLAWLESAGIEEVFVFC 77
Query: 62 CQNGQKIKEHVQKHQDNKSLWSLTMDIQILMSDTCQ---TMGDVMRELDAAAL----LKG 114
++ +++ N S W D + ++ Q + GD +R + + ++G
Sbjct: 78 ---SMQVIDYL-----NNSDWYSHKDFTVKTIESPQNSTSAGDALRYIYEQQIETSQIQG 129
Query: 115 YFVLAGINSITNMNFASLLEQHKQTCKKDKGTAMTLVYKKLSWEHPLISSDRSTFLAANE 174
FVL ++NM L+++H+ KKD+ MT+V ++ + +D F+A N
Sbjct: 130 DFVLVNGCIVSNMPLTQLIQEHRDRKKKDEKAIMTMVIRQ------SLITDHQLFIAVNP 183
Query: 175 NTKKVLVHKRYRPKSKEKIISLPLDLVLNNSEVKLHHNLVDTNIALCSPTVPPLFSDNFD 234
TK++L + E I L+ N V L ++ D I +CS V LF DNFD
Sbjct: 184 LTKQLLYY-------DEDNICFDKSLLDRNPSVLLCSDMQDCYIDICSLEVLSLFVDNFD 236
Query: 235 FQ-TRDDFIHGILINEEILASSLYYTLLKSNQYAAAITNWKTYQTV 279
+Q R DF+ G+L ++I+ + +T S+ YA+ I N+++Y V
Sbjct: 237 YQHMRCDFVEGVLA-DDIIGYKI-FTHEISSCYASRIENFRSYDMV 280
Score = 53.6 bits (123), Expect = 3e-07
Identities = 41/169 (24%), Positives = 80/169 (47%), Gaps = 13/169 (7%)
Query: 374 FLQEVIDSLARGYDEKLKCDYLILEINSSRYAYNIQ----LNEVNFFVVRALLSMPVLSE 429
F +EV + R +E + D +LEINS R +YN++ + + +++ +S P S
Sbjct: 510 FEREVDGTFLRAVEENIVADLAVLEINSLRLSYNMESAHCAGAIFYSMMKLAVSTP-HSS 568
Query: 430 AKNVLTTVKDILKYFRPVLANYIKSKSSIMDCLRAVEDTC-LNCEWLDGKSGQILHLLYE 488
++ I+ ++ +L Y+K ++ + +E+ C + L IL +YE
Sbjct: 569 INDLYRNASSIITRWKGLLGFYVKKSDEQIEVISRLEEMCEESAHELGTLFAHILRYMYE 628
Query: 489 A--DVVDEDSLVDWYAE---LKENANPFVKQPSLVKFFEWLQEASEESD 532
D++ E +++ W E E+ ++KQ F WL+E S++ D
Sbjct: 629 EENDLLQEVAILRWSDEKAGADESDKVYLKQ--CEPFITWLKETSDDED 675
>At1g36730.1 68414.m04569 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 439
Score = 46.4 bits (105), Expect = 5e-05
Identities = 24/76 (31%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 459 MDCLRAVEDTCLNCEWLDGKS-GQILHLLYEADVVDEDSLVDWYAELKENANPFVKQPSL 517
M L +E C+ K ++ LY+ D++DED +V+WY + ++P +K ++
Sbjct: 366 MGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYNK-GVKSSPVLK--NV 422
Query: 518 VKFFEWLQEASEESDD 533
F EWLQ A ES++
Sbjct: 423 TPFIEWLQNAESESEE 438
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 43.6 bits (98), Expect = 3e-04
Identities = 28/105 (26%), Positives = 53/105 (50%), Gaps = 5/105 (4%)
Query: 428 SEAKNVLTTVKDILKYFRPVLANYIKSKSSIMDCLRAVEDTCLNCEWLDGKSGQILHLLY 487
++ +N + ++ + K + P+L + S ++ + V+ C L +++ LY
Sbjct: 309 NQQQNANSVLRQV-KTWAPLLNTFCTSGKLELELMYKVQMQCYEDAKLMKVFPEVVRSLY 367
Query: 488 EADVVDEDSLVDWYAE--LKENANPFVKQPSLVKFFEWLQEASEE 530
E DV+ ED+++ W+ + + FVK SL F WL+EA EE
Sbjct: 368 ELDVLAEDTILHWFRKGTNSKGRQTFVK--SLEPFVNWLEEAEEE 410
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 43.2 bits (97), Expect = 4e-04
Identities = 27/93 (29%), Positives = 46/93 (49%), Gaps = 6/93 (6%)
Query: 441 LKYFRPVLANYIKSKSSIMDCLRAVEDTCLNCEWLDGKSGQILHLLYEADVVDEDSLVDW 500
+K + P+L + + M+ + V+ C L +++ LYE DV+ ED+++ W
Sbjct: 321 VKTWAPLLNTLCSTGNMEMELMYKVQMQCYEDAKLMKVFPEVVRSLYELDVLAEDTILHW 380
Query: 501 YAELKENANPFVKQ---PSLVKFFEWLQEASEE 530
Y ++ NP +Q L F WL+EA EE
Sbjct: 381 Y---RKGTNPKGRQTFVKGLEPFVNWLEEAEEE 410
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 41.1 bits (92), Expect = 0.002
Identities = 19/55 (34%), Positives = 32/55 (58%), Gaps = 1/55 (1%)
Query: 482 ILHLLYEADVVDEDSLVDWYAELKENANPFVKQPSLVK-FFEWLQEASEESDDSE 535
+L LY+ D+++E+ ++DWY + A+ VK F EWLQ A ES++ +
Sbjct: 383 VLKALYDQDIIEEEVVLDWYEKGLTGADKSSPVWKNVKPFVEWLQSAESESEEED 437
>At3g55590.1 68416.m06173 GDP-mannose pyrophosphorylase, putative
strong similarity to GDP-mannose pyrophosphorylase from
Arabidopsis thaliana [GI:3598958], Pichia angusta
[GI:7331158]; contains Pfam profile PF00483 Nucleotidyl
transferase
Length = 364
Score = 32.7 bits (71), Expect = 0.63
Identities = 15/51 (29%), Positives = 30/51 (58%)
Query: 9 VQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAIL 59
++A++++ F + PLT + P ++ A P+I + +E+L GV E +L
Sbjct: 1 MKALILVGGFGTRLRPLTLSLPKPLVDFANKPMILHQIEALKAIGVDEVVL 51
>At2g39770.1 68415.m04883 GDP-mannose pyrophosphorylase (GMP1)
identical to GDP-mannose pyrophosphorylase from
Arabidopsis thaliana [GI:3598958]; updated per Conklin
PL et al, PNAS 1999, 96(7):4198-203
Length = 361
Score = 32.7 bits (71), Expect = 0.63
Identities = 15/51 (29%), Positives = 30/51 (58%)
Query: 9 VQAVVIMDTFNSNFSPLTDNKPMGFLEVAGVPLIDYVLESLALGGVGEAIL 59
++A++++ F + PLT + P ++ A P+I + +E+L GV E +L
Sbjct: 1 MKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKAVGVDEVVL 51
>At3g17340.1 68416.m02216 importin-related contains Pfam profile
PF03810: Importin-beta N-terminal domain
Length = 822
Score = 30.3 bits (65), Expect = 3.4
Identities = 34/135 (25%), Positives = 60/135 (44%), Gaps = 11/135 (8%)
Query: 410 LNEVNFFVVRALLSMPVLSEAKNVLTTVKDILKYFRPVLANYI------KSKSSIMDCLR 463
+ + FV +A+L P S + L T+ ++++ V K S+M+ R
Sbjct: 637 VESIGSFVSKAILEYP--SATRRALMTLVKVIEHLLDVRHGNATDDLARKCFVSLMEASR 694
Query: 464 AVEDTC--LNCEWLDGKSGQILHLLYEADVVDEDSLVDWYAELKEN-ANPFVKQPSLVKF 520
+++ + + DG+ G+ E D DEDS D E +E + K + ++
Sbjct: 695 RLKEVNEETDDDEDDGEPGEEETESEETDSNDEDSESDECEETEEEFLERYAKVAAELED 754
Query: 521 FEWLQEASEESDDSE 535
E ++EA EE DD E
Sbjct: 755 SEVIEEADEEDDDHE 769
>At1g24330.1 68414.m03069 armadillo/beta-catenin repeat family
protein / U-box domain-containing family protein
contains Pfam domain, PF00514:
Armadillo/beta-catenin-like repeats and Pfam, PF04564:
U-box domain
Length = 771
Score = 29.9 bits (64), Expect = 4.4
Identities = 19/56 (33%), Positives = 32/56 (57%), Gaps = 6/56 (10%)
Query: 418 VRALLSMPV-LSEAKNVLTTVKDILKYFRP-----VLANYIKSKSSIMDCLRAVED 467
++ L S+ + L +AKN+L + K + VL + K+KS+++D LR VED
Sbjct: 51 IQTLCSLHIALEKAKNILQHCSECSKLYLAITGDAVLLKFEKAKSALIDSLRRVED 106
>At1g77680.1 68414.m09044 ribonuclease II family protein weak
similarity to SP|P37202 Mitotic control protein dis3
{Schizosaccharomyces pombe}; contains Pfam profile
PF00773: RNB-like protein
Length = 1055
Score = 29.1 bits (62), Expect = 7.7
Identities = 25/97 (25%), Positives = 48/97 (49%), Gaps = 10/97 (10%)
Query: 69 KEHVQKHQDNKSLWSLTMDIQILMSDTCQTMGDVMRELDAAALLKGYFVLAGINSITNMN 128
+E VQ+ +D + L LT+D T + D + + L G F G++ I +++
Sbjct: 470 EEEVQRRKDLRDLCVLTID-----PSTATDLDDAL----SVQSLPGGFFRVGVH-IADVS 519
Query: 129 FASLLEQHKQTCKKDKGTAMTLVYKKLSWEHPLISSD 165
+ L E T + + T++ L+ +K+S PL+S +
Sbjct: 520 YFVLPETALDTEARFRSTSVYLMQRKISMLPPLLSEN 556
>At1g75140.1 68414.m08728 expressed protein
Length = 617
Score = 29.1 bits (62), Expect = 7.7
Identities = 19/62 (30%), Positives = 27/62 (43%)
Query: 129 FASLLEQHKQTCKKDKGTAMTLVYKKLSWEHPLISSDRSTFLAANENTKKVLVHKRYRPK 188
F + LE + T + + T ++KL PLI+SDR L V +K P
Sbjct: 406 FPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDREKLLVMGLGDGYVATYKSKLPI 465
Query: 189 SK 190
SK
Sbjct: 466 SK 467
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.390
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,498,119
Number of Sequences: 28952
Number of extensions: 459637
Number of successful extensions: 1149
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1120
Number of HSP's gapped (non-prelim): 20
length of query: 535
length of database: 12,070,560
effective HSP length: 84
effective length of query: 451
effective length of database: 9,638,592
effective search space: 4347004992
effective search space used: 4347004992
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 62 (29.1 bits)
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