BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001194-TA|BGIBMGA001194-PA|IPR001412|Aminoacyl-tRNA synthetase, class I, IPR000924|Glutamyl-tRNA synthetase, class Ic, IPR007639|Glutaminyl-tRNA synthetase, non-specific RNA-binding region part 1, IPR007638|Glutaminyl-tRNA synthetase, non-specific RNA-binding region part 2, IPR005113|uDENN, IPR001194|DENN, IPR011035|Ribosomal protein L25-like (2195 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_1632| Best HMM Match : No HMM Matches (HMM E-Value=.) 365 e-100 SB_24326| Best HMM Match : DENN (HMM E-Value=0) 283 8e-76 SB_11708| Best HMM Match : Kunitz_BPTI (HMM E-Value=7.2) 108 4e-23 SB_8676| Best HMM Match : Peptidase_M18 (HMM E-Value=0) 84 1e-15 SB_26794| Best HMM Match : DENN (HMM E-Value=1.4e-38) 63 2e-09 SB_50069| Best HMM Match : tRNA-synt_1c (HMM E-Value=3e-31) 57 1e-07 SB_59772| Best HMM Match : dDENN (HMM E-Value=1.6e-24) 50 2e-05 SB_7895| Best HMM Match : uDENN (HMM E-Value=5.7e-25) 43 0.003 SB_50003| Best HMM Match : PAN (HMM E-Value=0.044) 34 1.1 SB_9796| Best HMM Match : uDENN (HMM E-Value=1.1e-35) 33 1.9 SB_25427| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 3.4 >SB_1632| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 700 Score = 365 bits (899), Expect = e-100 Identities = 164/231 (70%), Positives = 193/231 (83%), Gaps = 11/231 (4%) Query: 242 GENYKSDGYVVTSHTKRLLEEHLKITGGKVRTRFPPEPNGILHIGHAKAININFGYAAAH 301 G+NY + GYVVT T +LL+EHL+ T G+V+TRFPPEPNGILHIGHAKAININFGYA AH Sbjct: 170 GDNYHTPGYVVTPKTMQLLKEHLERTKGRVQTRFPPEPNGILHIGHAKAININFGYAKAH 229 Query: 302 DGVCFLRYDDTNPXXXXXXXXIGIKDMVEWLGYKPYKITHSSDYFDQLYEWAIQLIKKDL 361 +GVC+LRYDDTNP GI DMV+WLG++P+KITH+SD FD+LYE+A++LIK+ Sbjct: 230 NGVCYLRYDDTNPEKEEEKFFTGILDMVKWLGFEPWKITHASDNFDKLYEYAVELIKRGD 289 Query: 362 AYVCHQKSEDIKGFNPPPSPWRIRPIEESLQLFEDMKNGKIDEGDATLRMKITLEEGKQD 421 AYVCHQ+ E+IKG+NPPPSPWR RPIEESL+LFE ATLRMK TLE+GK D Sbjct: 290 AYVCHQQYEEIKGYNPPPSPWRERPIEESLRLFE-----------ATLRMKTTLEDGKAD 338 Query: 422 PVAYRIRFKPHHRTGNKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQSRRA 472 PVAYRI+F PHHR+G+KWCIYPTYD+THCLCDSIE ITHSLCTKEFQSRR+ Sbjct: 339 PVAYRIKFTPHHRSGDKWCIYPTYDFTHCLCDSIEDITHSLCTKEFQSRRS 389 Score = 263 bits (645), Expect = 1e-69 Identities = 123/192 (64%), Positives = 150/192 (78%), Gaps = 2/192 (1%) Query: 471 RAMVVLEPLKIRIVNFPSEQPISIQVPDFPSEPERGSHSVTFDKVIYIEATDFKEEMEKG 530 R M VLEPLK+ I NFPS++ + I+VPD P + +G+H V FD+V+YIE++DF+E MEKG Sbjct: 491 RVMAVLEPLKVTITNFPSDKAMMIEVPDLPMDASKGNHKVPFDRVLYIESSDFRETMEKG 550 Query: 531 YRRLTPTQSVGLRHAGYVIKVSKVIKDPSGKVLELECTAESTDSSEKPKAFIHWV-SEPI 589 YRRLTP QSVGLRHAGYVIKV KVIK+ SG+V ELE T E T S++KPKAFIHWV S + Sbjct: 551 YRRLTPEQSVGLRHAGYVIKVKKVIKNASGEVSELEVTCELTSSTDKPKAFIHWVASTAV 610 Query: 590 SIEVRLYEPLFKHKNPEDPNEVPGGFLSDCRDDTLKVM-EAYADSSIKGAKVYDKFQFER 648 E+RLY+ LFKHKNPEDP EVPGGF+SDC D+L V+ A D + KGA + KFQFER Sbjct: 611 PCEIRLYDRLFKHKNPEDPEEVPGGFVSDCNKDSLTVIPNALVDITAKGAAPFTKFQFER 670 Query: 649 IGFFSVDPDSSQ 660 G+F VDPDSS+ Sbjct: 671 QGYFCVDPDSSK 682 Score = 71.7 bits (168), Expect = 6e-12 Identities = 38/118 (32%), Positives = 71/118 (60%), Gaps = 2/118 (1%) Query: 10 FKLLGLNEQKAKETLKNANVTKVLLAALDEVQLQS-LPLGAGILIYHLATKIKPQTMSKL 68 F+ +GL++QKAKET+KN +++ L + + + +G L+Y LA+ + S++ Sbjct: 8 FQSIGLSQQKAKETMKNEALSQKLEKIVTQAHSKGESDKQSGTLLYVLASSSLKEE-SRV 66 Query: 69 NFICQYIASGKLDSTLRVDAALDYLLGNINEANVNIEEFEKACGVGVIVTPEQVEQAV 126 + + YI S K+ S+ ++ AA +YL ++ +FE+ CG+GV +TPEQ+E+ + Sbjct: 67 SLLVDYICSKKVSSSAQLTAAFEYLTKTSPVGPIDACKFEQHCGIGVEITPEQIEECI 124 >SB_24326| Best HMM Match : DENN (HMM E-Value=0) Length = 1281 Score = 283 bits (695), Expect = 8e-76 Identities = 165/394 (41%), Positives = 233/394 (59%), Gaps = 35/394 (8%) Query: 774 WSVLTGQAYDNTPTIVLHDVKEIETWILRLLSAPVPVPGKTRLELEVLSPTAHSPLLFAL 833 WSV T D + + + EIETWI RLL AP PV G+T++++E+L P ++ L FA Sbjct: 181 WSVFTCSE-DPKLSPIATQMDEIETWIQRLLLAPAPVIGRTKVDMELLCPESYPSLTFAY 239 Query: 834 PDHTRFTLVDFPLHLPLELLGVDTCLKVLTLIILENKIVVQSRDYNALSMSVMALVAMLY 893 P++TRF L+DFP+H+P+ELLGV+TCLKVLT I+LE K+V+QSRDYNAL+MSV+AL +LY Sbjct: 240 PENTRFPLIDFPVHIPMELLGVETCLKVLTCILLEQKVVIQSRDYNALTMSVIALTRLLY 299 Query: 894 PLEYMFPAIPLLPSCMSCAEQLLLAPTPFLIGIPATFLTYK-KHFKLPDDIWLVDLDATK 952 PLEYMFP IPLLP+CM+ AEQLLLAPTP++IG+PA+F YK K F++P D+W+ DLD++K Sbjct: 300 PLEYMFPIIPLLPTCMANAEQLLLAPTPYVIGVPASFFRYKSKDFQIPGDLWVFDLDSSK 359 Query: 953 LISPTGNDQDLPPLPEPEGSVLKSHLKQALNSLT---NTTAEQASALLMPSRRDSVGGAT 1009 + P D +LP LPE EG VL LK AL +L+ T +A D+ G Sbjct: 360 MTVPMSAD-ELPDLPEEEGQVLIRRLKMALQALSMPPQTVGNLENAAPDGLLPDNTSG-D 417 Query: 1010 LKVQPTWYKGGAESAPHSAAESRRVS--VTGTASAG---HTPQRHSLASPHGAAQPFNPL 1064 ++ G + A V+ ++ T G HT P A Q P Sbjct: 418 ASIEEHMVFGNDVDSVDVAVRVAIVNFFMSPTVLCGLQEHTRTLRLFPRPVVALQK-GPF 476 Query: 1065 IYGNDVDSVDVATRV-AMAVEFLAEWSLTPCNVAFLRV---------------------Q 1102 + S VA + +VE+ AEW ++P N A+L++ + Sbjct: 477 LKSRPKPSDFVAALAESQSVEYFAEWLVSPTNTAYLKINKEQLTRSGQRSVIGFFFVNFR 536 Query: 1103 TGVFDPRQIGDKPKWFADQLQPIRFAVWDDGSSL 1136 G+++P IGDKPKW+++ L+ + + V+D S L Sbjct: 537 LGIYNPCVIGDKPKWYSEHLETVLYQVYDKESKL 570 Score = 99 bits (238), Expect = 2e-20 Identities = 43/80 (53%), Positives = 55/80 (68%), Gaps = 5/80 (6%) Query: 656 PDSSQTHAPELLRRYPLTNHDDFPLPLDMVYFCQPEGCVSVGPRRAPGPFASRDTTSFVF 715 P T P LLRR+P +HDDFPLP D+V+FCQPE C +V + F+ R+ SF+F Sbjct: 25 PTLDSTETPRLLRRFPEKDHDDFPLPPDVVFFCQPEACNTVSKK-----FSLREANSFIF 79 Query: 716 TLTDKDSGKTRYGICVNFYR 735 TLTDKD+ K RYG+C+N YR Sbjct: 80 TLTDKDTNKVRYGVCMNIYR 99 Score = 74.5 bits (175), Expect = 9e-13 Identities = 32/51 (62%), Positives = 40/51 (78%), Gaps = 1/51 (1%) Query: 1812 TARTYLYQGLIGKERSNLWDQMQFWEDAFLDAVSQERDMIGMDQGANEMME 1862 T R YL++GL+ +ERS LWD M FWE+ FLDAV+ ERD +GMDQG EM+E Sbjct: 1231 TGRRYLFEGLV-RERSPLWDNMDFWENIFLDAVAAERDAVGMDQGPTEMLE 1280 Score = 53.2 bits (122), Expect = 2e-06 Identities = 22/45 (48%), Positives = 34/45 (75%) Query: 1603 KGMLKVLQAVVHGLEYTYSNFGLGGMASVFQLAEMAHTHYWSREL 1647 KGML++L+AVV G E + N G+GG+AS F+ E+ HT+Y+ R++ Sbjct: 978 KGMLEILRAVVAGYEASVVNHGIGGVASAFKFLEIIHTNYYGRDI 1022 >SB_11708| Best HMM Match : Kunitz_BPTI (HMM E-Value=7.2) Length = 156 Score = 108 bits (260), Expect = 4e-23 Identities = 47/86 (54%), Positives = 60/86 (69%) Query: 1971 VRDDGLVLRSTQGTIVERWWYERLVNMTYSPKIRVLCLWRKNGGQTQLHKYYTKKCKALY 2030 V DD ++LR+ G I+ERWWYE++VN+TY PK +VLCLW + G T L K+ TKKCKALY Sbjct: 51 VSDDCILLRTGAGVIIERWWYEKMVNITYCPKTKVLCLWYRRGEDTLLSKFCTKKCKALY 110 Query: 2031 YCIKEAMERSGRRQDAAELGGEFPVQ 2056 +CIKE M+R+ R G E VQ Sbjct: 111 FCIKECMQRAAERLRGPNTGTEPRVQ 136 >SB_8676| Best HMM Match : Peptidase_M18 (HMM E-Value=0) Length = 1161 Score = 84.2 bits (199), Expect = 1e-15 Identities = 43/105 (40%), Positives = 59/105 (56%) Query: 269 GKVRTRFPPEPNGILHIGHAKAININFGYAAAHDGVCFLRYDDTNPXXXXXXXXIGIKDM 328 G V TRFPPE +G LHIGHAKA +N Y ++G +R+DDTNP I Sbjct: 16 GNVVTRFPPEASGYLHIGHAKAALLNQYYKNMYEGTLIMRFDDTNPAKENAHFEEVILKD 75 Query: 329 VEWLGYKPYKITHSSDYFDQLYEWAIQLIKKDLAYVCHQKSEDIK 373 +E L +K K TH+SD+FD L + ++++ D AY E +K Sbjct: 76 LEMLEFKFDKFTHTSDHFDTLIGYCEKMLRMDKAYADDTAPERMK 120 >SB_26794| Best HMM Match : DENN (HMM E-Value=1.4e-38) Length = 595 Score = 63.3 bits (147), Expect = 2e-09 Identities = 41/106 (38%), Positives = 58/106 (54%), Gaps = 7/106 (6%) Query: 850 LELLGVDTCLKVLTLIILENKIVVQSRDYNALSMSVMALVAMLYPLEYMFPAIPLLPSCM 909 L LG D CL + LI+ E+KI++ S L+ AL +L+P + P IPL P + Sbjct: 326 LRHLGPDNCLLLFYLILTEHKILIHSLRPALLTSVAEALTNVLFPFTWQCPYIPLCPVAL 385 Query: 910 SCAEQLLLAPTPFLIGIPATFLTYKKHFKLPDDIWLVDLDATKLIS 955 + +L AP PF+IGI + Y + PDD+ VDLD T +IS Sbjct: 386 N---DVLCAPCPFIIGIDS---RYFDQYDPPDDVTSVDLD-TIMIS 424 Score = 31.9 bits (69), Expect = 5.9 Identities = 12/34 (35%), Positives = 21/34 (61%) Query: 661 THAPELLRRYPLTNHDDFPLPLDMVYFCQPEGCV 694 T+ ++L R+P +++ FPLP + FC P G + Sbjct: 210 TYHADILSRFPEKDYEGFPLPEAVPMFCLPLGAI 243 >SB_50069| Best HMM Match : tRNA-synt_1c (HMM E-Value=3e-31) Length = 210 Score = 57.2 bits (132), Expect = 1e-07 Identities = 29/77 (37%), Positives = 42/77 (54%), Gaps = 3/77 (3%) Query: 397 MKNGKIDEGDATLRMKITLEEGK---QDPVAYRIRFKPHHRTGNKWCIYPTYDYTHCLCD 453 MK G +R KI + +DP YR + + H RTGNK+ +YP YD+ + D Sbjct: 1 MKRGTEYGQSCCIRAKIDYQSPNGTLRDPTIYRCKPETHIRTGNKYKVYPIYDFACPIVD 60 Query: 454 SIEHITHSLCTKEFQSR 470 S+E +TH+L T E+ R Sbjct: 61 SLEGVTHALRTTEYHDR 77 >SB_59772| Best HMM Match : dDENN (HMM E-Value=1.6e-24) Length = 423 Score = 50.4 bits (115), Expect = 2e-05 Identities = 33/123 (26%), Positives = 61/123 (49%), Gaps = 11/123 (8%) Query: 888 LVAMLYPLEYMFPAIPLLPSCMSCAEQLLLAPTPFLIGIPATFLTYKKHFKLPDD--IWL 945 +V +++P + +P+LP+ + + L AP PF++G+ +K + +LP + + L Sbjct: 12 VVTLIFPFIWQHVYVPILPAAL---QHFLDAPVPFIMGLYCASEDFKANLQLPSEASLCL 68 Query: 946 VDLDATKLISPTGNDQDLPPLPEPEGSVLKSHLKQALNSLTNTTAEQASALLMPSRRDSV 1005 VD+D K++SP +DLP P + L L + + + E + L SR S Sbjct: 69 VDIDNCKVLSP----EDLPEFPNKQD--LIEELLETIEKFNIASQESNNPLSSNSRCSSE 122 Query: 1006 GGA 1008 G + Sbjct: 123 GNS 125 >SB_7895| Best HMM Match : uDENN (HMM E-Value=5.7e-25) Length = 945 Score = 42.7 bits (96), Expect = 0.003 Identities = 20/59 (33%), Positives = 37/59 (62%), Gaps = 3/59 (5%) Query: 868 ENKIVVQSRDYNALSMSVMALVAMLYPLEYMFPAIPLLPSCMSCAEQLLLAPTPFLIGI 926 +NKI+ S + L+ A++A++YPL++ + IP+LP+ + L APTP ++G+ Sbjct: 249 KNKILFISSSFVQLTTVTRAMLALMYPLKFSYVYIPILPAALL---DFLRAPTPTIMGV 304 Score = 33.9 bits (74), Expect = 1.5 Identities = 25/83 (30%), Positives = 37/83 (44%), Gaps = 11/83 (13%) Query: 654 VDPDSSQTHA--PELLRRYPLTNHDDFPLPLDMVYFCQPEGCVSVGPRRAPGPFASRDTT 711 +D D+S ++L+R+P + D P + FCQP G + + R T Sbjct: 13 IDKDTSYGEGLKGKVLQRFPPQDRKDVAFPEGIELFCQPTGWKT--------SYYCRPQT 64 Query: 712 SFVFTLTDKDSGKTRYGICVNFY 734 FV LTD + G +Y C FY Sbjct: 65 FFVSVLTDME-GDHKYLACFTFY 86 >SB_50003| Best HMM Match : PAN (HMM E-Value=0.044) Length = 286 Score = 34.3 bits (75), Expect = 1.1 Identities = 35/128 (27%), Positives = 50/128 (39%), Gaps = 5/128 (3%) Query: 961 QDLPPLPEPEGSVLKSHLKQALNSLTNTTAEQASALLMPSRRDSVGGATLKVQPTWYKGG 1020 Q LPP P+P + K+ Q L L N T+ A+ P ++D +T+ T Sbjct: 54 QQLPPAPQPTANPEKAEEPQPLKPLDNYTSAAAAVPSTPPKQDP--RSTVAFGRTTKSSP 111 Query: 1021 AESAPHSAAESRRVSVTGTASAGHTPQRHSLASPHGA-AQPFNPLI-YGNDVDSVDVATR 1078 + +P RR S+ T Q A P +P N +I N D + V T Sbjct: 112 VQKSPSRVGHVRRYSLENDQVICSTKQVAGRARPISTQIKPRNTVITRTNSADRLHV-TS 170 Query: 1079 VAMAVEFL 1086 M FL Sbjct: 171 ARMLWAFL 178 >SB_9796| Best HMM Match : uDENN (HMM E-Value=1.1e-35) Length = 300 Score = 33.5 bits (73), Expect = 1.9 Identities = 26/82 (31%), Positives = 42/82 (51%), Gaps = 8/82 (9%) Query: 796 IETWILRLL-SAPVPVPGKT-RLELEVLSPTAHSPLLFALPDHTRFTLVDFPLHLPLELL 853 +E++I LL P+P PG+T R S T P LP L D+ L L Sbjct: 223 LESYIYNLLYEVPLPPPGRTMRFNCVKESITCQRPGPNELP------LFDYSLKEMFSWL 276 Query: 854 GVDTCLKVLTLIILENKIVVQS 875 GV+ +++ T ++LE++I++ S Sbjct: 277 GVENVVELFTCVLLEHQILLLS 298 >SB_25427| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1198 Score = 32.7 bits (71), Expect = 3.4 Identities = 37/142 (26%), Positives = 57/142 (40%), Gaps = 8/142 (5%) Query: 917 LAPTPFLIGIPATFLTYKKHFKLPDDIWLVDLDATKLISPTGNDQDLPPLPEPEGSVLKS 976 + P ++G T + K KL +I+L D DAT + P Q P P K Sbjct: 741 VTPKEIMVGGTMTGYYFVKEEKLNLNIFLQDQDAT-IDIPVSTTQTTPE-PIYARPYKKR 798 Query: 977 HLKQALNSLTNTTAEQASALLMPSRRDSVGGATLKVQPTWYKGGAESAPHSAAESR---R 1033 Q + + TN ++ L+ RD + +W S+ HS+ +SR R Sbjct: 799 KSAQLVRAPTNNGLKEVE-LIAKVSRDENSRSQSTSSSSW--STTTSSNHSSVQSRGPMR 855 Query: 1034 VSVTGTASAGHTPQRHSLASPH 1055 VS+ S +P S +S H Sbjct: 856 VSIHSVTSKPRSPGSTSTSSNH 877 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.316 0.133 0.390 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 66,755,439 Number of Sequences: 59808 Number of extensions: 2708234 Number of successful extensions: 5562 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 5532 Number of HSP's gapped (non-prelim): 26 length of query: 2195 length of database: 16,821,457 effective HSP length: 94 effective length of query: 2101 effective length of database: 11,199,505 effective search space: 23530160005 effective search space used: 23530160005 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 68 (31.5 bits)
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