GeneModel BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001194-TA|BGIBMGA001194-PA|IPR001412|Aminoacyl-tRNA
synthetase, class I, IPR000924|Glutamyl-tRNA synthetase, class Ic,
IPR007639|Glutaminyl-tRNA synthetase, non-specific RNA-binding region
part 1, IPR007638|Glutaminyl-tRNA synthetase, non-specific RNA-binding
region part 2, IPR005113|uDENN, IPR001194|DENN, IPR011035|Ribosomal
protein L25-like
         (2195 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po...   338   5e-93
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe...   164   1e-40
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce...    47   3e-05
SPAPB1A10.11c |||glutamyl-tRNA synthetase, mitochondrial|Schizos...    42   0.001
SPCC1840.07c |||phosphoprotein phosphatase |Schizosaccharomyces ...    30   4.2  
SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces pomb...    29   5.5  
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    29   5.5  

>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 811

 Score =  338 bits (831), Expect = 5e-93
 Identities = 200/481 (41%), Positives = 267/481 (55%), Gaps = 34/481 (7%)

Query: 4   EEIVERFKLLGLNEQKAKETLKNANVTKVLLAALDEVQLQSLPLGA--GILIYHLATKIK 61
           EE+  +F  +GLNE   K+TLKN  ++  L   ++E  + S       G L++ LA    
Sbjct: 6   EELRAKFTKIGLNETTVKDTLKNKKLSSSLNKVIEETNVGSSGCDRTIGNLLFTLANASL 65

Query: 62  PQTMSKLN----FICQYIASGKLDSTLRVDAALDYLLGNINEANVNIEEFEKACGVGVIV 117
            Q   K N    FI   I SG L + L+V+AA+ Y     ++  ++  EF+K  GVGV++
Sbjct: 66  KQKDPKSNAHEAFIASKIVSGDLKTNLQVNAAITYCK---DKDTIDESEFDKETGVGVVL 122

Query: 118 TPEQVEQAVEKHMVKYKQELLEKRYRF-NSGIIMQAVRADLTWADGKAIKSEVDVQIFDL 176
           TPEQ+EQ V  ++ + K ++LE+RY+  N         A L WA    +K  +D +  +L
Sbjct: 123 TPEQIEQLVGDYVAENKSKILEQRYQLLNPSASALRQHALLKWAPQLEVKQTLDRKFLEL 182

Query: 177 LGPKTEADLXXXXXXXXXXXXGDXXXXXXXXXXXXXXQENVESSEDIGGATSISELMKKL 236
           LGPKTE D                             ++ + S +           + KL
Sbjct: 183 LGPKTEQDAAAGKKKGAKAKNSKQKTVDSGKAK----EQKIVSEQSKKYNMFEEGFLAKL 238

Query: 237 PFHAPGENYKSDGYVVTSHTKRLLEEHLKITGGKVRTRFPPEPNGILHIGHAKAININFG 296
             H PG N        T      ++EHL+ TGG V TRFPPEPNG LHIGH+KAI +NFG
Sbjct: 239 --HKPGGN--------TQLIPERMKEHLQATGGGVVTRFPPEPNGYLHIGHSKAIAVNFG 288

Query: 297 YAAAHDGVCFLRYDDTNPXXXXXXXXIGIKDMVEWLGYKPYKITHSSDYFDQLYEWAIQL 356
           +A  H+GVC+LR+DDTNP          IKD+V WLG++PYKIT+SSDYFD+LYE A +L
Sbjct: 289 FARYHNGVCYLRFDDTNPEAEEERYFESIKDLVAWLGFQPYKITYSSDYFDKLYELAEEL 348

Query: 357 IKKDLAYVCHQKSEDIK----GFNPPP---SPWRIRPIEESLQLFEDMKNGKIDEGDATL 409
           IK+D AYVCH    +IK    G    P      R RPIEESL  F +M++GK    +A L
Sbjct: 349 IKRDKAYVCHCTDAEIKKARGGEERGPRYACVHRDRPIEESLLEFRNMRDGKYQPKEAIL 408

Query: 410 RMKITLEEGKQ---DPVAYRIRFKPHHRTGNKWCIYPTYDYTHCLCDSIEHITHSLCTKE 466
           RMK  L +G     D +AYR+   PH RTG+KW IYPTYD+THCL DS E+I+HSLCT E
Sbjct: 409 RMKQDLSDGNPQMWDLIAYRVLNSPHPRTGDKWKIYPTYDFTHCLVDSFENISHSLCTTE 468

Query: 467 F 467
           F
Sbjct: 469 F 469



 Score = 92.7 bits (220), Expect = 5e-19
 Identities = 56/162 (34%), Positives = 85/162 (52%), Gaps = 11/162 (6%)

Query: 454 SIEHITHSLCTKEFQSR---RAMVVLEPLKIRIVNFPSEQPISIQVPDFPSEPERGSHSV 510
           +IE      C ++F      R M + +P+K+ + N        I++P  P +P  GS S 
Sbjct: 556 NIEVARFENCVRKFLENSVPRLMFLPDPIKVTLENLDDSYREQIEIPFNPKDPSMGSRSA 615

Query: 511 TFDKVIYIEATDFKEEMEKGYRRLTPTQSVGLRHAGYVIKVSKVIKDPSGKVLELECTAE 570
              K IYI+ +DF+EE    + RLT  Q VGL  A + +   +V+K+  G+ +E+    +
Sbjct: 616 FLTKHIYIDRSDFREEASSDFFRLTLGQPVGLFRASHPVVAKRVVKNDEGEPIEIIAEYD 675

Query: 571 STDSSEKPKAFIHWVS------EPISI-EVRLYEPLFKHKNP 605
           ++ SS+KPK FI WVS       P+ I E RL+  LFK  NP
Sbjct: 676 AS-SSKKPKTFIQWVSRDKESNSPVLIAETRLFNNLFKCDNP 716


>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 716

 Score =  164 bits (398), Expect = 1e-40
 Identities = 86/210 (40%), Positives = 120/210 (57%), Gaps = 5/210 (2%)

Query: 269 GKVRTRFPPEPNGILHIGHAKAININFGYAAAHDGVCFLRYDDTNPXXXXXXXXIGIKDM 328
           GKV TRFPPEP+G LHIGHAKA  +N  +A  + G   +R+DDTNP          I + 
Sbjct: 205 GKVVTRFPPEPSGYLHIGHAKAALLNQYFANKYHGKLIVRFDDTNPSKENSEFQDAILED 264

Query: 329 VEWLGYKPYKITHSSDYFDQLYEWAIQLIKKDLAYVCHQKSEDIKGFNPP--PSPWRIRP 386
           V  LG KP  +T++SDY D ++++ + +IK   AY      E ++       PS  R RP
Sbjct: 265 VALLGIKPDVVTYTSDYLDTIHQYCVDMIKSGQAYADDTDVETMRHERTEGIPSKHRDRP 324

Query: 387 IEESLQLFEDMKNGKIDEGDATLRMKITLE---EGKQDPVAYRIRFKPHHRTGNKWCIYP 443
           IEESL++  +M  G        +R KI+ E   +  +DPV YR    PHHRTG K+  YP
Sbjct: 325 IEESLEILSEMDKGSDVGLKNCIRAKISYENPNKAMRDPVIYRCNLLPHHRTGTKYRAYP 384

Query: 444 TYDYTHCLCDSIEHITHSLCTKEFQSRRAM 473
           TYD+   + DS+E +TH+L T E++ R  +
Sbjct: 385 TYDFACPIVDSLEGVTHALRTTEYRDRNPL 414



 Score = 36.3 bits (80), Expect = 0.048
 Identities = 47/186 (25%), Positives = 71/186 (38%), Gaps = 13/186 (6%)

Query: 479 LKIRIVNFPSEQPISIQVPDFPSEPERGSHSVTFDKVIYIEATDFKEEMEKGYRRLTPTQ 538
           +K  IVN P+  P +   P     PE G+    F   I IE  D +   +         +
Sbjct: 525 VKATIVNGPAA-PYAEDRPRHKKNPELGNKKSIFANEILIEQADAQSFKQD--------E 575

Query: 539 SVGLRHAGYVIKVSKVIKDPSGKVLELECTAESTDSSEKPKAFIHWVSEPIS-IEVRLYE 597
            V L   G    V ++ +D SGKV  L+         +K +  + W+++      V L +
Sbjct: 576 EVTLMDWGNAY-VREINRDASGKVTSLKLELHLDGDFKKTEKKVTWLADTEDKTPVDLVD 634

Query: 598 PLFKHKNPEDPNEVPGGFLSDCRDDTLKVMEAYADSSIKGAKVYDKFQFERIGFFSVDPD 657
             F +   +D  E    +       T      +AD  IK  K  D  Q ER G++ VD  
Sbjct: 635 --FDYLITKDKLEEGENYKDFLTPQTEFHSPVFADVGIKNLKKGDIIQVERKGYYIVDVP 692

Query: 658 SSQTHA 663
              T A
Sbjct: 693 FDGTQA 698


>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 46.8 bits (106), Expect = 3e-05
 Identities = 63/224 (28%), Positives = 102/224 (45%), Gaps = 22/224 (9%)

Query: 767 AFSRD---TVWSVLTGQAYDNTPTIVLHDVKEIETW-ILRLLSAPVPVPGKTRLELEV-L 821
           +F RD    V S +  +  D  P+  L+ +K +     ++ +   VP+P K  L+L+V +
Sbjct: 378 SFCRDWLRIVCSSIQAEDLDFIPSSDLNSLKSLNLQSFVKNICCDVPLPPKGLLQLQVNV 437

Query: 822 SPTAHSPLLFALPDHTRFTLVDFPLHLPLELLGVDTCLKVLTLIILENKIVVQSRDYNAL 881
            P   +   F  P +      D  L+     L +   L +    ++E K++  S +   L
Sbjct: 438 GPL--NLYAFRSPVNEIPGWNDVDLYPLFRALSIPNILVLFEAALMEAKVIFLSENLGML 495

Query: 882 SMSVMALVAMLYPLEYMFPAIPLLP-SCMSCAEQLLLAPTPFLIGIPATFLTYKKHF-KL 939
             +  AL+ +LYPL +    IP+LP   +SC E    AP  ++IG     L+Y  H   +
Sbjct: 496 GYASQALLHLLYPLTWQGLYIPVLPRRLISCFE----APCSYIIGT----LSYFFHMDDV 547

Query: 940 P-DDIWLVDLDATK-LISPTGNDQDLPPLPEPEGSVLKSHLKQA 981
           P D+I LV  D  K  +S  G    +  L     S L++HLK A
Sbjct: 548 PLDNIPLVVCDLDKNSVSTFGK---IVRLSRSLRSKLQAHLKLA 588


>SPAPB1A10.11c |||glutamyl-tRNA synthetase,
           mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 526

 Score = 41.5 bits (93), Expect = 0.001
 Identities = 18/43 (41%), Positives = 25/43 (58%)

Query: 271 VRTRFPPEPNGILHIGHAKAININFGYAAAHDGVCFLRYDDTN 313
           VRTRF P P G LH+G  +    N+ +A   +G   LR +DT+
Sbjct: 34  VRTRFAPSPTGFLHLGSLRTALFNYLWAKKSNGKFILRLEDTD 76


>SPCC1840.07c |||phosphoprotein phosphatase |Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 332

 Score = 29.9 bits (64), Expect = 4.2
 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 6/45 (13%)

Query: 1014 PTWYKGGAESAPHSAAESRRVSVTGTASA-----GHTPQRHSLAS 1053
            P WY+G A+ +   A E   ++VT T +      GHTPQ H + S
Sbjct: 243  PMWYRGLAQLSEEEACEVA-LNVTKTLNVNRLVMGHTPQFHGIVS 286


>SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1018

 Score = 29.5 bits (63), Expect = 5.5
 Identities = 20/89 (22%), Positives = 42/89 (47%), Gaps = 2/89 (2%)

Query: 976  SHLKQALNSLTNTTAEQASALLMPSRRDSVGGATLKVQPTWYKGGAESAPHS-AAESRRV 1034
            S+ + + N+L +   E   + L PS+++ +  +  K+    +    +++ H+ A  SR +
Sbjct: 532  SNSRPSSNALKSDANENTDSSLPPSKKEFIEKSLHKLSEPLHDDSRQNSDHNFAPHSRPI 591

Query: 1035 SV-TGTASAGHTPQRHSLASPHGAAQPFN 1062
            ++   TAS     Q     + +GAA   N
Sbjct: 592  AIRVATASQRELEQTEKRKAKNGAANASN 620


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 29.5 bits (63), Expect = 5.5
 Identities = 22/86 (25%), Positives = 43/86 (50%), Gaps = 4/86 (4%)

Query: 80  LDSTLRVDAALDYLLGNINEANVNIEEFEKACGVGVIVTPEQVEQAVEKHMVKYKQELL- 138
           +D T R +  L + L +I +AN N+   E+      IV+  + EQ ++ H+  Y    + 
Sbjct: 258 VDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVS--EYEQLLQFHVATYMDNEIA 315

Query: 139 -EKRYRFNSGIIMQAVRADLTWADGK 163
            + +    SG  ++++RA L   +G+
Sbjct: 316 GQPQALQKSGRPLKSIRARLKGKEGR 341


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.316    0.133    0.390 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,101,755
Number of Sequences: 5004
Number of extensions: 373712
Number of successful extensions: 922
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 904
Number of HSP's gapped (non-prelim): 13
length of query: 2195
length of database: 2,362,478
effective HSP length: 84
effective length of query: 2111
effective length of database: 1,942,142
effective search space: 4099861762
effective search space used: 4099861762
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 61 (28.7 bits)