Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001194-TA|BGIBMGA001194-PA|IPR001412|Aminoacyl-tRNA
synthetase, class I, IPR000924|Glutamyl-tRNA synthetase, class Ic,
IPR007639|Glutaminyl-tRNA synthetase, non-specific RNA-binding region
part 1, IPR007638|Glutaminyl-tRNA synthetase, non-specific RNA-binding
region part 2, IPR005113|uDENN, IPR001194|DENN, IPR011035|Ribosomal
protein L25-like
(2195 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g25350.1 68414.m03145 glutamine-tRNA ligase, putative / gluta... 382 e-105
At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / gluta... 142 2e-33
At1g49040.2 68414.m05499 stomatal cytokinesis defective / SCD1 p... 72 4e-12
At1g49040.1 68414.m05498 stomatal cytokinesis defective / SCD1 p... 72 4e-12
At5g19720.1 68418.m02345 tRNA synthetase class I (E and Q) famil... 67 1e-10
At5g64050.1 68418.m08042 glutamate-tRNA ligase family protein / ... 50 2e-05
At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein ... 42 0.004
At2g06210.1 68415.m00684 phosphoprotein-related low similarity t... 36 0.31
At1g72950.1 68414.m08437 disease resistance protein (TIR-NBS cla... 33 2.9
At5g13000.1 68418.m01490 glycosyl transferase family 48 protein ... 31 6.6
At4g20130.1 68417.m02945 ribulose-1,5 bisphosphate carboxylase/o... 31 6.6
At4g33630.2 68417.m04778 expressed protein 31 8.7
At4g33630.1 68417.m04777 expressed protein 31 8.7
At1g71710.1 68414.m08289 inositol polyphosphate 5-phosphatase, p... 31 8.7
>At1g25350.1 68414.m03145 glutamine-tRNA ligase, putative /
glutaminyl-tRNA synthetase, putative / GlnRS, putative
similar to tRNA-glutamine synthetase GI:2995454 from
[Lupinus luteus]
Length = 795
Score = 382 bits (941), Expect = e-105
Identities = 197/476 (41%), Positives = 289/476 (60%), Gaps = 14/476 (2%)
Query: 4 EEIVERFKLLGLNEQKAKETLKNANVTKVLLAALDEVQL-QSLPLGAGILIYHLATKIKP 62
E+ +E F +GL+E+ A+ T+ N VT L A + E + G L+Y +ATK
Sbjct: 9 EKSIELFISIGLDEKTARNTINNNKVTANLTAVIHEAAVTDGCDRNTGNLLYSVATKFPT 68
Query: 63 QTMSKLNFICQYIASGKLDSTLRVDAALDYLLGNINEANVNIEEFEKACGVGVIVTPEQV 122
+ + +YI + K+ + +++AA + E + + EFE+ACGVG+ V+PE +
Sbjct: 69 NALVHRPTLLKYIVNSKIKTPAQLEAAFAFFASTGPE-DFKLNEFEEACGVGIEVSPEDI 127
Query: 123 EQAVEKHMVKYKQELLEKRYRFNSGIIMQAVRADLTWADGKAIKSEVDVQIFDLLGPKTE 182
E+AV+ + K+ +LE+RYR N G + VR L WAD K +K +D ++++LLG KT
Sbjct: 128 EKAVKGIFEENKKTILEQRYRTNVGELFGHVRKSLPWADPKIVKKLIDEKMYELLGEKTA 187
Query: 183 ADLXXXXXXXXXXXXGDXXXXXXXXXXXXXXQENVESSEDIGGATSISELMKKLPFHAPG 242
AD S E++ T + + H
Sbjct: 188 ADNEKPTKKKEKKEKPAKVEEKKAVVETTAEP----SEEELNPYTIFPQPEQNFMVHT-- 241
Query: 243 ENYKSDGYVVT-SHTKRLLEEHLKITGGKVRTRFPPEPNGILHIGHAKAININFGYAAAH 301
E + SDG ++ S+TK +L++HLK+TGGKV TRFPPEPNG LHIGHAKA+ ++FG A
Sbjct: 242 EVFFSDGSILRCSNTKEVLDKHLKVTGGKVYTRFPPEPNGYLHIGHAKAMFVDFGLAKER 301
Query: 302 DGVCFLRYDDTNPXXXXXXXXIGIKDMVEWLGYKPYKITHSSDYFDQLYEWAIQLIKKDL 361
G C+LRYDDTNP I+++V+W+G++P+KIT++SDYF +LY+ A++LI++
Sbjct: 302 GGCCYLRYDDTNPEAEKEEYINHIEEIVKWMGWEPFKITYTSDYFQELYDLAVELIRRGH 361
Query: 362 AYVCHQKSEDIKGFNPPP--SPWRIRPIEESLQLFEDMKNGKIDEGDATLRMKITLEE-- 417
AYV HQ +++IK + SPWR RPIEESL+LF++M+ G I+EG ATLRMK ++
Sbjct: 362 AYVDHQTADEIKEYREKKMNSPWRDRPIEESLKLFDEMRRGIIEEGKATLRMKQDMQSDN 421
Query: 418 -GKQDPVAYRIRFKPHHRTGNKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQSRRA 472
D +AYRI+F PH + G+KWCIYP+YDY HC DS+E+ITHSLCT EF++RRA
Sbjct: 422 FNMYDLIAYRIKFAPHPKAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRA 477
Score = 125 bits (302), Expect = 3e-28
Identities = 77/199 (38%), Positives = 117/199 (58%), Gaps = 16/199 (8%)
Query: 471 RAMVVLEPLKIRIVNFPSEQPISIQV---PDFPSEPERGSHSVTFDKVIYIEATDFKEEM 527
R MVVL PLK+ I N S++ I + PD ++ + V F +V+YI+ +DF+ +
Sbjct: 580 RTMVVLNPLKVVITNLESDKLIELDAKRWPDAQNDDPSAFYKVPFSRVVYIDQSDFRMKD 639
Query: 528 EKGYRRLTPTQSVGLRHAGYVIKVSKVI-KDPSGKVLELECTAESTDSSEKPKAFIHWVS 586
K Y L P +SV LR+A + IK + V+ D + V E+ + S KPK +HWV+
Sbjct: 640 SKDYYGLAPGKSVLLRYA-FPIKCTNVVFADDNETVREIHAEYDPEKKS-KPKGVLHWVA 697
Query: 587 E------PISIEVRLYEPLFKHKNPEDPNEVPGGFLSDCRDDTLKVME-AYADSSIKGAK 639
E PI +EVRL+E LF +NP + N+ +L+D ++ V+ AYA S++K A
Sbjct: 698 ESSPGEEPIKVEVRLFEKLFNSENPAELNDA---WLTDINPNSKMVISGAYAVSTLKDAA 754
Query: 640 VYDKFQFERIGFFSVDPDS 658
V D+FQFER+G+++VD DS
Sbjct: 755 VGDRFQFERLGYYAVDKDS 773
>At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative /
glutamyl-tRNA synthetase, putatuve / GluRS, putative
identical to gi:3435196
Length = 719
Score = 142 bits (345), Expect = 2e-33
Identities = 83/223 (37%), Positives = 124/223 (55%), Gaps = 7/223 (3%)
Query: 269 GKVRTRFPPEPNGILHIGHAKAININFGYAAAHDGVCFLRYDDTNPXXXXXXXXIGIKDM 328
GKV+ RF PEP+G LHIGHAKA +N +A + G +R+DDTNP +
Sbjct: 212 GKVKLRFAPEPSGYLHIGHAKAALLNKYFAERYQGEVIVRFDDTNPAKESNEFVDNLVKD 271
Query: 329 VEWLGYKPYKITHSSDYFDQLYEWAIQLIKKDLAYVCHQKSEDI--KGFNPPPSPWRIRP 386
+ LG K K+T++SDYF +L + A +L+++ AYV E + + + S R
Sbjct: 272 IGTLGIKYEKVTYTSDYFPELMDMAEKLMREGKAYVDDTPREQMQKERMDGIDSKCRNHS 331
Query: 387 IEESLQLFEDMKNGKIDEGDATLRMKITLEE---GKQDPVAYRIRFKPHHRTGNKWCIYP 443
+EE+L+L+++M G +R K +++ +DPV YR HHR G+K+ IYP
Sbjct: 332 VEENLKLWKEMIAGSERGLQCCVRGKFNMQDPNKAMRDPVYYRCNPMSHHRIGDKYKIYP 391
Query: 444 TYDYTHCLCDSIEHITHSLCTKEFQSRRAMV--VLEPLKIRIV 484
TYD+ DS+E ITH+L + E+ R A VLE + +R V
Sbjct: 392 TYDFACPFVDSLEGITHALRSSEYHDRNAQYFKVLEDMGLRQV 434
Score = 48.8 bits (111), Expect = 4e-05
Identities = 49/205 (23%), Positives = 84/205 (40%), Gaps = 16/205 (7%)
Query: 470 RRAMVVLEPLKIRIVNFPSEQPISIQVPDFPSEPERGSHSVTFDKVIYIEATDFKEEMEK 529
R VV E + + ++P +P G + TF K I++E D
Sbjct: 522 RHTAVVAERRVLFTLTDGPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASA---- 577
Query: 530 GYRRLTPTQSVGLRHAGYVIKVSKVIKDPSGKVLELECTAESTDSSEKPKAFIHWV---S 586
++ + V L G I V ++ KD G+V L S + K + W+ +
Sbjct: 578 ----ISVGEEVTLMDWGNAI-VKEITKDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTN 632
Query: 587 EPISIEVRLYEPLFKHKNPEDPNEVPGGFLSDCRDDTLKVMEAYADSSIKGAKVYDKFQF 646
E +++ + ++ L K ED +EV + + +TL A DS+++ K D Q
Sbjct: 633 ELVNLTLTEFDYLITKKKLEDDDEVADFVNPNTKKETL----ALGDSNMRNLKCGDVIQL 688
Query: 647 ERIGFFSVDPDSSQTHAPELLRRYP 671
ER G+F D ++ P +L P
Sbjct: 689 ERKGYFRCDVPFVKSSKPIVLFSIP 713
>At1g49040.2 68414.m05499 stomatal cytokinesis defective / SCD1
protein (SCD1) contains Pfam PF02141: DENN (AEX-3)
domain; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies); identical to stomatal cytokinesis defective
[Arabidopsis thaliana] GI:19743728; supporting cDNA
gi|19743727|gb|AY082605.1|; PMID 12874123
Length = 909
Score = 72.1 bits (169), Expect = 4e-12
Identities = 47/179 (26%), Positives = 90/179 (50%), Gaps = 14/179 (7%)
Query: 805 SAPVPVPGKTRLELEVLSPTAHSPLLFALPDHTRFTLVDFPLHLPLELLGVDTCLKVLTL 864
+ P+P PGK R VL + L P D L ++ L VD +K+ T
Sbjct: 170 NVPLPTPGKDR----VLFAVENCLLSVEAPPEDSLPQADISLQPLVQCLDVDNLIKLFTS 225
Query: 865 IILENKIVVQSRDYNALSMSVMALVAMLYPLEYMFPAIPLLPSCMSCAEQLLLAPTPFLI 924
+++E +I+++S Y+ L++ ++ ++YP ++ IPLL + APTP+++
Sbjct: 226 VLVERRILIRSNKYSLLTLVSESICHLIYPFRWLQVYIPLL---FFSGVDYIDAPTPYMM 282
Query: 925 GIPATFLTYKKHFKLPDDIWLVDLDATKLISPTGNDQDLPPLPEPEGSVLKSHLKQALN 983
G+ + T D + +VDLD ++ + +++P +PEPE S L++ + + L+
Sbjct: 283 GLHSDVDTSNLAM---DGVVVVDLDINQITT----SEEIPQIPEPEFSTLRNDILKLLH 334
>At1g49040.1 68414.m05498 stomatal cytokinesis defective / SCD1
protein (SCD1) contains Pfam PF02141: DENN (AEX-3)
domain; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies); identical to stomatal cytokinesis defective
[Arabidopsis thaliana] GI:19743728; supporting cDNA
gi|19743727|gb|AY082605.1|; PMID 12874123
Length = 1187
Score = 72.1 bits (169), Expect = 4e-12
Identities = 47/179 (26%), Positives = 90/179 (50%), Gaps = 14/179 (7%)
Query: 805 SAPVPVPGKTRLELEVLSPTAHSPLLFALPDHTRFTLVDFPLHLPLELLGVDTCLKVLTL 864
+ P+P PGK R VL + L P D L ++ L VD +K+ T
Sbjct: 170 NVPLPTPGKDR----VLFAVENCLLSVEAPPEDSLPQADISLQPLVQCLDVDNLIKLFTS 225
Query: 865 IILENKIVVQSRDYNALSMSVMALVAMLYPLEYMFPAIPLLPSCMSCAEQLLLAPTPFLI 924
+++E +I+++S Y+ L++ ++ ++YP ++ IPLL + APTP+++
Sbjct: 226 VLVERRILIRSNKYSLLTLVSESICHLIYPFRWLQVYIPLL---FFSGVDYIDAPTPYMM 282
Query: 925 GIPATFLTYKKHFKLPDDIWLVDLDATKLISPTGNDQDLPPLPEPEGSVLKSHLKQALN 983
G+ + T D + +VDLD ++ + +++P +PEPE S L++ + + L+
Sbjct: 283 GLHSDVDTSNLAM---DGVVVVDLDINQITT----SEEIPQIPEPEFSTLRNDILKLLH 334
>At5g19720.1 68418.m02345 tRNA synthetase class I (E and Q) family
protein similar to tRNA-glutamine synthetase [Lupinus
luteus] GI:2995455; contains Pfam profile PF03950: tRNA
synthetases class I (E and Q), anti-codon binding domain
Length = 170
Score = 66.9 bits (156), Expect = 1e-10
Identities = 48/154 (31%), Positives = 74/154 (48%), Gaps = 12/154 (7%)
Query: 497 PDFPSEPERGSHSVTFDKVIYIEATDFKEEMEKGYRRLTPTQSVGLRHAGYVIKVSKVI- 555
PD P++ + V +VIY+E +DF+ E K Y L P +SV LR+ + IK + V+
Sbjct: 9 PDAPNDDPSAFYKVPLSRVIYMEQSDFQNEDSKNYYGLAPGKSVLLRYT-FPIKCTNVVF 67
Query: 556 KDPSGKVLELECTAESTDSSEKPKAFIHWV------SEPISIEVRLYEPLFKHKNPEDPN 609
D + V E+ + + KPK +HWV EP +EV +E LF N E+P
Sbjct: 68 ADDTKTVCEIYVEYD-PEKKIKPKGVLHWVPEYSPGKEPTKVEVCSFENLF---NSENPA 123
Query: 610 EVPGGFLSDCRDDTLKVMEAYADSSIKGAKVYDK 643
E+ +L+D SI G V+++
Sbjct: 124 ELNDDWLTDINPQLQSGYYTVDKDSIPGKLVFNR 157
>At5g64050.1 68418.m08042 glutamate-tRNA ligase family protein /
glutamyl-tRNA synthetase family protein similar to
SP|P22250 from Bacillus subtilis, SP|P22249 from
Bacillus stearothermophilus; contains Pfam tRNA
synthetases class I (E and Q), catalytic domain PF00749
Length = 570
Score = 49.6 bits (113), Expect = 2e-05
Identities = 23/66 (34%), Positives = 32/66 (48%)
Query: 268 GGKVRTRFPPEPNGILHIGHAKAININFGYAAAHDGVCFLRYDDTNPXXXXXXXXIGIKD 327
GG VR RF P P G LH+G A+ N+ +A + G LR +DT+ +
Sbjct: 51 GGSVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTRESEAAVLQ 110
Query: 328 MVEWLG 333
+ WLG
Sbjct: 111 DLSWLG 116
>At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein
contains Pfam domain PF02141: DENN (AEX-3) domain; this
cDNA may contain an anomalously spliced intron...will
require further examination.
Length = 765
Score = 42.3 bits (95), Expect = 0.004
Identities = 32/116 (27%), Positives = 61/116 (52%), Gaps = 11/116 (9%)
Query: 853 LGVDTCLKVLTLIILENKIVVQSRDYNALSMSVMALVAMLYPLEYMFPAIPLLPSCMSCA 912
L +D L +L +LE +IV + L+ SV++++ ++ P + +P+LP M
Sbjct: 547 LRLDNVLMILAGALLEKQIVFVCSNLGILAASVLSIIPVIRPFRWQSLLMPVLPDDML-- 604
Query: 913 EQLLLAPTPFLIGIPATFLTYKKHFKLPDDIWLVDLDATKLISPTGNDQDLPPLPE 968
+ L AP P+++G+ T + KL + I +VD+ ++ SP+ +P LP+
Sbjct: 605 -EFLDAPVPYIVGVKNK--TSEVQSKLTNVI-VVDILKNQVKSPS-----MPQLPQ 651
>At2g06210.1 68415.m00684 phosphoprotein-related low similarity to
phosphoprotein from Mus musculus GI:1236239; contains
Pfam profile PF00515 TPR Domain
Length = 1064
Score = 35.9 bits (79), Expect = 0.31
Identities = 34/105 (32%), Positives = 45/105 (42%), Gaps = 7/105 (6%)
Query: 1813 ARTYLYQGLIGKERSNLWDQMQFWEDAFLDAVSQERDMIGMDQGANEMMERYKCLSETER 1872
AR Y QG I + R L + D + V ER I N + Y L +TE
Sbjct: 47 AREYFKQGKIEQFRQILEEGSSSDIDEYYADVKYERIAI-----LNALGAYYSYLGKTET 101
Query: 1873 KRLEHEEDRLLSTALYNLTAAMVLFGVEPDTIRNKVRRLLAKSHI 1917
K E EE + +T YN + + + EP T K + LLAK I
Sbjct: 102 KNREKEEQFISATRYYNKASRIDMH--EPSTWVGKGQLLLAKGEI 144
>At1g72950.1 68414.m08437 disease resistance protein (TIR-NBS class),
putative domain signature TIR-NBS exists, suggestive of a
disease resistance protein.
Length = 379
Score = 32.7 bits (71), Expect = 2.9
Identities = 18/67 (26%), Positives = 37/67 (55%), Gaps = 3/67 (4%)
Query: 1993 RLVNMTYSPKIRVLCLWRKNG-GQTQLHKY-YTKKCKAL-YYCIKEAMERSGRRQDAAEL 2049
RL+N+ +RV+ +W + G G++ L K+ Y C+ +C E+++R + + + L
Sbjct: 198 RLLNLNSKKSVRVIGIWARGGNGRSALAKFVYQNICQHFESHCFLESVKRISQDRHLSHL 257
Query: 2050 GGEFPVQ 2056
EF ++
Sbjct: 258 HEEFMIR 264
>At5g13000.1 68418.m01490 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1889
Score = 31.5 bits (68), Expect = 6.6
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Query: 1847 ERDMIGMDQGANEMMERYKCLSETERKRLEHEEDRLLSTALY 1888
E D++ D+ N +ER KCLSE E K + E+ L A Y
Sbjct: 1165 EPDLLSPDEW-NNFLERVKCLSEEELKESDELEEELRLWASY 1205
>At4g20130.1 68417.m02945 ribulose-1,5 bisphosphate
carboxylase/oxygenase large subunit
N-methyltransferase-related contains weak similarity to
Swiss-Prot:P94026 ribulose-1,5 bisphosphate
carboxylase/oxygenase large subunit N-
methyltransferase, chloroplast precursor (Ribulose-
bisphosphate-carboxylase]-lysine N-methyltransferase,
RuBisCO methyltransferase, RuBisco LSMT, rbcMT)
[Nicotiana tabacum]
Length = 483
Score = 31.5 bits (68), Expect = 6.6
Identities = 18/44 (40%), Positives = 22/44 (50%), Gaps = 2/44 (4%)
Query: 956 PTGNDQDLPPLPEPEGSVLKSHLKQALNSLTN--TTAEQASALL 997
PT +D DLPP+P E +K + L TTAEQ LL
Sbjct: 400 PTWSDIDLPPIPSAERKAVKELQDECRKMLAEYPTTAEQDQKLL 443
>At4g33630.2 68417.m04778 expressed protein
Length = 684
Score = 31.1 bits (67), Expect = 8.7
Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Query: 87 DAALDYLLGNINEANVNIEEFEKACGVGVIVTPEQVEQAVEKHMVKYKQELLEKRYR 143
D L L +N A + E++E A + V + AV K M + + LLE+RY+
Sbjct: 127 DRLLSVLKSQLNRA-IKREDYEDAARLKVAIAATATNDAVGKVMSTFYRALLEERYK 182
>At4g33630.1 68417.m04777 expressed protein
Length = 684
Score = 31.1 bits (67), Expect = 8.7
Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Query: 87 DAALDYLLGNINEANVNIEEFEKACGVGVIVTPEQVEQAVEKHMVKYKQELLEKRYR 143
D L L +N A + E++E A + V + AV K M + + LLE+RY+
Sbjct: 127 DRLLSVLKSQLNRA-IKREDYEDAARLKVAIAATATNDAVGKVMSTFYRALLEERYK 182
>At1g71710.1 68414.m08289 inositol polyphosphate 5-phosphatase,
putative similar to inositol polyphosphate 5-phosphatase
I [Arabidopsis thaliana] GI:10444261
Length = 664
Score = 31.1 bits (67), Expect = 8.7
Identities = 11/29 (37%), Positives = 18/29 (62%)
Query: 368 KSEDIKGFNPPPSPWRIRPIEESLQLFED 396
K +K ++ PPSP R +P EE+ + E+
Sbjct: 202 KLSGVKSYSDPPSPGRFKPFEETHDIIEE 230
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.133 0.390
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 47,478,329
Number of Sequences: 28952
Number of extensions: 1978246
Number of successful extensions: 4916
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 4885
Number of HSP's gapped (non-prelim): 24
length of query: 2195
length of database: 12,070,560
effective HSP length: 93
effective length of query: 2102
effective length of database: 9,378,024
effective search space: 19712606448
effective search space used: 19712606448
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 67 (31.1 bits)
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