BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001188-TA|BGIBMGA001188-PA|IPR001247|Exoribonuclease, IPR012462|Protein of unknown function DUF1671 (805 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe... 166 9e-42 SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|c... 99 1e-21 SPAC3G9.10c |ski6||exosome subunit Ski6 |Schizosaccharomyces pom... 36 0.017 SPAC25H1.04 |mug105||DUF1671 family protein|Schizosaccharomyces ... 34 0.089 SPBC2A9.03 |||conserved protein |Schizosaccharomyces pombe|chr 2... 30 1.1 SPAC1002.01 ||SPAC1610.05|conserved fungal protein|Schizosacchar... 30 1.5 SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa... 29 2.5 SPBC336.09c |rrn7||RNA polymerase I transcription factor subunit... 29 3.4 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 29 3.4 SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosa... 29 3.4 SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 28 4.4 SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces... 28 4.4 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 28 5.9 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 7.8 SPAC19B12.08 |||peptidase family C54|Schizosaccharomyces pombe|c... 27 7.8 >SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe|chr 2|||Manual Length = 270 Score = 166 bits (404), Expect = 9e-42 Identities = 86/243 (35%), Positives = 146/243 (60%), Gaps = 7/243 (2%) Query: 4 VYKLIHPVKHFNDYISIKTRLDGRNFDEHRNIKLNVNSIKTADASAVVKCGNTTVVCGIK 63 ++K I P ++ + ++ R DGR+ E R I +N N I TA+ SA+++ G VCGIK Sbjct: 16 IFKKITPEQYLSHLLNQDVRSDGRSVSEFREIVINDNCISTANGSAIIRAGENVFVCGIK 75 Query: 64 LELAVPKAEEPDLGFLITNVELPALCSSKYRPGPPSDYAQVINNIVSEIIKNSKCIDLKD 123 E+A P P+ G+++ N+EL LCSSK++PGPPSD AQV++ + + ++ S I+L+ Sbjct: 76 AEIAEPFENSPNEGWIVPNLELSPLCSSKFKPGPPSDLAQVVSQELHQTLQQSNLINLQS 135 Query: 124 LCIVPDKLAWVVYCDMVCIDHDGSLVDACIIALIASFKTLLLPSVNYDTETEEIKVETNE 183 LCI K AWV+Y D++C+++DGS D AL A+ KT+ LP+ +D + E + + Sbjct: 136 LCIFEKKAAWVLYADIICLNYDGSAFDYAWAALFAALKTVKLPTAVWDEDLERVICASTL 195 Query: 184 RINLKIHGLPVATSFAVYEHFQSQIILLTDPTNYEEEMCGGNGSNLIISYNKGLLCGIQK 243 +++ + S++V++ LL DPT+ EE++ ++++ +K ++ I K Sbjct: 196 TRPVQLSTEVRSFSWSVFDD-----KLLADPTDEEEDL-STEFLTIMLNSSKNIV-KIIK 248 Query: 244 LGG 246 LGG Sbjct: 249 LGG 251 >SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|chr 3|||Manual Length = 291 Score = 99 bits (238), Expect = 1e-21 Identities = 66/226 (29%), Positives = 108/226 (47%), Gaps = 11/226 (4%) Query: 23 RLDGRNFDEHRNIKLNVNSIKTADASAVVKCGNTTVVCGIKLELAVPKAEEPDLGFLITN 82 RLDGR + R++++ V G+T V+ I E+ P + P G Sbjct: 24 RLDGRQLSDFRSLEIQFGK---EYGQVDVSFGHTRVMARITTEITKPYTDRPFDGIFSIT 80 Query: 83 VELPALCSSKYRPGPPSDYAQVINNIVSEIIKNSKCIDLKDLCIVPDKLAWVVYCDMVCI 142 EL L S + G SD VI+ ++ + ++ S +D + LCI+ + W V + I Sbjct: 81 TELTPLAYSAFEAGRVSDQEIVISRLIEKAVRRSNALDTESLCIISGQKCWHVRASVHFI 140 Query: 143 DHDGSLVDACIIALIASFKTLLLPSVNYDTETEEI-KVETNERINLKIHGLPVATSFAVY 201 +HDG+LVDA IA+IA+ P + E + VE + L I +P+ +F+ + Sbjct: 141 NHDGNLVDAACIAVIAALCHFRRPELTVVGEEVTVHPVEERVPVPLSILHMPICVTFSFF 200 Query: 202 EHFQSQIILLTDPTNYEEEMCGGNGSNLIISYNKGL-LCGIQKLGG 246 + + I+ D T EE++C G ++ I+ NK +C I K GG Sbjct: 201 NNGELAIV---DATLEEEDLCNG---SMTITLNKNREVCQIYKAGG 240 >SPAC3G9.10c |ski6||exosome subunit Ski6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 36.3 bits (80), Expect = 0.017 Identities = 18/51 (35%), Positives = 31/51 (60%), Gaps = 2/51 (3%) Query: 11 VKHFNDYISIK-TRLDGRNFDEHRNIKLNVNSIKTADASAVVKCGNTTVVC 60 + HF + +S++ R DGR +DE RN + + + + SA ++ GNT V+C Sbjct: 1 MSHF-EILSLEGLRNDGRRWDEMRNFQCRIGIEPSENGSAFIELGNTKVLC 50 >SPAC25H1.04 |mug105||DUF1671 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 244 Score = 33.9 bits (74), Expect = 0.089 Identities = 10/20 (50%), Positives = 15/20 (75%) Query: 633 DDGWGCAYRSMQTIFSWFRY 652 D GWGC +R++Q + SW +Y Sbjct: 37 DRGWGCGWRNIQMLISWLQY 56 >SPBC2A9.03 |||conserved protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 30.3 bits (65), Expect = 1.1 Identities = 19/65 (29%), Positives = 36/65 (55%), Gaps = 5/65 (7%) Query: 244 LGGGSLSTEN--NEEAIRLAE-QHTIAVEKRLSKIDTTESTGCLYGLMYDGTLLVVGFSL 300 + S+ EN ++ ++++ + ++ I +K +S +DTT + GCL G +Y G + F Sbjct: 152 VSNNSIRMENTLHDNSVQIIDLKNNILQKKAISCLDTTNAVGCL-GTLY-GQYCIFNFKN 209 Query: 301 ESFET 305 SF T Sbjct: 210 GSFST 214 >SPAC1002.01 ||SPAC1610.05|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 29.9 bits (64), Expect = 1.5 Identities = 11/39 (28%), Positives = 24/39 (61%) Query: 357 IIINREKDMKAHFIVHDKFEETKYEVFSPEELWKQFLHV 395 I++N+ K + FI+ +K + + + + S +LWK+ L + Sbjct: 31 ILLNKRKFHYSPFILQEKVQSSNHTIRSDTKLWKRLLKI 69 >SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1076 Score = 29.1 bits (62), Expect = 2.5 Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 3/50 (6%) Query: 9 HPVKHFN---DYISIKTRLDGRNFDEHRNIKLNVNSIKTADASAVVKCGN 55 HPV ++ +Y++ + R F EH+N+K+ V S SA K N Sbjct: 529 HPVILYDPIHEYVNHELRKRENEFSEHKNVKIFVASYNLNGCSATTKLEN 578 >SPBC336.09c |rrn7||RNA polymerase I transcription factor subunit Rrn7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 537 Score = 28.7 bits (61), Expect = 3.4 Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 1/59 (1%) Query: 501 VKTAVKMMTTQRKPAFYISMPLRIDTLAMIHRNTKLL-DLYIVLVEAACRSLRLMESVL 558 +K + KM ++RK A+ +P +T HR T ++ LY + +++ ++R +ES L Sbjct: 471 IKESDKMDFSRRKDAYISMLPFSPNTENTAHRVTIMVAKLYNIKLDSLIAAIRYVESCL 529 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 28.7 bits (61), Expect = 3.4 Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 2/54 (3%) Query: 443 ASDVGLTGTSTEATVGELVDSMSPEQPSKKKKYNINNAEILPVSLILKVTKDIL 496 ASD G + + + V ++ P S Y+IN+A+ P S +KV ++IL Sbjct: 342 ASDKRKEGFTEKEETADAVVTLVPSHSSSPVNYSINSAKSTPAS--IKVNEEIL 393 >SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1215 Score = 28.7 bits (61), Expect = 3.4 Identities = 19/71 (26%), Positives = 30/71 (42%) Query: 260 LAEQHTIAVEKRLSKIDTTESTGCLYGLMYDGTLLVVGFSLESFETETEKNSYSQLLLNF 319 + E+ ++V K + + TG L G D L V ++ +T T SYS L Sbjct: 784 IKEKQKVSVPKGAYLLGVADETGTLKGHYDDAVLSVPEIFIQITDTSTSFGSYSTGKLKT 843 Query: 320 PAEIELCGVVK 330 + LC V + Sbjct: 844 RVIVGLCIVAR 854 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 28.3 bits (60), Expect = 4.4 Identities = 17/53 (32%), Positives = 28/53 (52%) Query: 58 VVCGIKLELAVPKAEEPDLGFLITNVELPALCSSKYRPGPPSDYAQVINNIVS 110 ++ +K ++ AE+ + L T + AL SS + PS YAQ IN ++S Sbjct: 617 IIAELKKKVEENGAEDRSVKDLATILYETALLSSGFTLDDPSAYAQRINRLIS 669 >SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 298 Score = 28.3 bits (60), Expect = 4.4 Identities = 16/59 (27%), Positives = 27/59 (45%) Query: 38 NVNSIKTADASAVVKCGNTTVVCGIKLELAVPKAEEPDLGFLITNVELPALCSSKYRPG 96 +V I + V CG V +K +A ++P+ FL+TN + + K+ PG Sbjct: 141 DVEKIGPDPSVGAVLCGMDMHVTYLKYCMAFQYLQDPNCAFLLTNQDSTFPTNGKFLPG 199 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 27.9 bits (59), Expect = 5.9 Identities = 12/35 (34%), Positives = 19/35 (54%) Query: 299 SLESFETETEKNSYSQLLLNFPAEIELCGVVKFGE 333 + ++ E E EK + +L NF I+ CG V G+ Sbjct: 745 TFDTLEEEYEKTVATDILRNFAESIKTCGPVVLGD 779 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 27.5 bits (58), Expect = 7.8 Identities = 13/46 (28%), Positives = 24/46 (52%) Query: 496 LSDKLVKTAVKMMTTQRKPAFYISMPLRIDTLAMIHRNTKLLDLYI 541 +SDKLV TAV + + FY P +D + +++ L+ ++ Sbjct: 819 VSDKLVNTAVLFINIELLSEFYYQFPRELDQRLIHSLSSEQLNAFV 864 >SPAC19B12.08 |||peptidase family C54|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 27.5 bits (58), Expect = 7.8 Identities = 9/22 (40%), Positives = 15/22 (68%) Query: 627 MQDNFNDDGWGCAYRSMQTIFS 648 +++ +D GWGC RS QT+ + Sbjct: 63 LENMTSDTGWGCMIRSTQTLLA 84 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,732,284 Number of Sequences: 5004 Number of extensions: 161193 Number of successful extensions: 444 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 430 Number of HSP's gapped (non-prelim): 16 length of query: 805 length of database: 2,362,478 effective HSP length: 79 effective length of query: 726 effective length of database: 1,967,162 effective search space: 1428159612 effective search space used: 1428159612 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 58 (27.5 bits)
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