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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001172-TA|BGIBMGA001172-PA|IPR001206|Diacylglycerol
kinase, catalytic region, IPR002219|Protein kinase C, phorbol
ester/diacylglycerol binding
         (269 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_42132| Best HMM Match : DAGK_cat (HMM E-Value=1.3e-35)             125   3e-29
SB_56638| Best HMM Match : DAGK_cat (HMM E-Value=7.8e-19)              99   2e-21
SB_58879| Best HMM Match : No HMM Matches (HMM E-Value=.)              80   2e-15
SB_52560| Best HMM Match : No HMM Matches (HMM E-Value=.)              71   8e-13
SB_58173| Best HMM Match : No HMM Matches (HMM E-Value=.)              39   0.004
SB_30938| Best HMM Match : DAGK_acc (HMM E-Value=1.4013e-45)           36   0.026
SB_32644| Best HMM Match : zf-C2H2 (HMM E-Value=0)                     31   1.3  
SB_45123| Best HMM Match : zf-C2H2 (HMM E-Value=0)                     29   3.0  
SB_37930| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.0  
SB_32903| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.0  
SB_45463| Best HMM Match : zf-C2H2 (HMM E-Value=0)                     28   7.0  
SB_57| Best HMM Match : zf-C2H2 (HMM E-Value=0)                        28   7.0  
SB_37978| Best HMM Match : zf-C2H2 (HMM E-Value=0)                     28   7.0  
SB_21417| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.0  
SB_47786| Best HMM Match : Ank (HMM E-Value=4.4e-30)                   28   9.3  
SB_7580| Best HMM Match : Topoisom_bac (HMM E-Value=0)                 28   9.3  
SB_6589| Best HMM Match : zf-C2H2 (HMM E-Value=1.2e-36)                28   9.3  
SB_576| Best HMM Match : RVT_1 (HMM E-Value=0)                         28   9.3  
SB_9849| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   9.3  

>SB_42132| Best HMM Match : DAGK_cat (HMM E-Value=1.3e-35)
          Length = 558

 Score =  125 bits (302), Expect = 3e-29
 Identities = 92/271 (33%), Positives = 123/271 (45%), Gaps = 47/271 (17%)

Query: 1   MKCSACKIIAHTACIDILMDRVQFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKA 60
           + C  C +  H  C++   +   FTCK           R  ++ HH+          C  
Sbjct: 167 LHCENCTLSVHDECLETANE--VFTCKALVLS------RRSSLKHHYIRGNLPLCSYCSV 218

Query: 61  CGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQRIGEECSLGSQSNITVPPSWIV 120
           CG      L  ++  +    C WC++  H  + C   Q +   C  G    + +PP  I 
Sbjct: 219 CGC-----LCGTAPRLCDQRCIWCQEKVH--DDCLRNQSM--ICDFGRYRTLILPPHCI- 268

Query: 121 KLPRRGXXXXXXXXXXXXXXXXXXXXXXXXNELRTFVIKPIP---TTNVKPVLVFINPKS 177
                                         N+ R FV+K I    T N  P+LVF N KS
Sbjct: 269 ---------------------SLTLVGWRKNKQR-FVVKEITPPNTRNWSPLLVFANCKS 306

Query: 178 GGNQGAKLLQKFQWLLNPRQVFDLTQGGPGPGLELFRKVP--NLRVLACGGDGTVGWVLS 235
           G N G +LLQ F+ +LNP QV DL +  P   LE  R +P    RVL CGGDG+VGWVL 
Sbjct: 307 GDNDGERLLQAFRGVLNPVQVIDLHEVPPETALEFCRLLPGHRCRVLVCGGDGSVGWVLD 366

Query: 236 VLDRIGSR--PAVGVLPLGTGNDLARALGWG 264
            LD++  +  P +G+LPLGTGNDLAR LGWG
Sbjct: 367 ALDKVKLKLSPYIGILPLGTGNDLARVLGWG 397


>SB_56638| Best HMM Match : DAGK_cat (HMM E-Value=7.8e-19)
          Length = 836

 Score =   99 bits (238), Expect = 2e-21
 Identities = 44/62 (70%), Positives = 52/62 (83%), Gaps = 2/62 (3%)

Query: 206 PGPGLELFRKVPNLRVLACGGDGTVGWVLSVLDRI--GSRPAVGVLPLGTGNDLARALGW 263
           P P LEL+RKVPN+R+LACGGDGTVGW+LS LD++    RP V +LPLGTGNDL+RAL W
Sbjct: 166 PVPTLELYRKVPNVRLLACGGDGTVGWILSELDKLKFNPRPPVAILPLGTGNDLSRALNW 225

Query: 264 GG 265
           GG
Sbjct: 226 GG 227


>SB_58879| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1786

 Score = 80.2 bits (189), Expect = 2e-15
 Identities = 42/90 (46%), Positives = 53/90 (58%), Gaps = 19/90 (21%)

Query: 168 PVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPG-------------------P 208
           P+LVF+N KSG NQG K ++KF+ LLNP QVFDL  GGP                    P
Sbjct: 856 PLLVFVNSKSGDNQGVKFIRKFKQLLNPLQVFDLGVGGPTNGVYESFAIHYELINIIFIP 915

Query: 209 GLELFRKVPNLRVLACGGDGTVGWVLSVLD 238
            L+ FR+    R+L CGGDG+VGWV+  +D
Sbjct: 916 SLKFFRRFRRFRILVCGGDGSVGWVMKEVD 945



 Score = 41.9 bits (94), Expect = 5e-04
 Identities = 16/19 (84%), Positives = 18/19 (94%)

Query: 246  VGVLPLGTGNDLARALGWG 264
            +GVLPLGTGNDL+R LGWG
Sbjct: 995  IGVLPLGTGNDLSRVLGWG 1013


>SB_52560| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1263

 Score = 71.3 bits (167), Expect = 8e-13
 Identities = 31/60 (51%), Positives = 41/60 (68%), Gaps = 5/60 (8%)

Query: 210  LELFRKVPNLRVLACGGDGTVGWVLSVLDRIGS-----RPAVGVLPLGTGNDLARALGWG 264
            L  F+ +P  R+L CGGDGTVGWVLS LD + +     +P   ++PLGTGND++R L WG
Sbjct: 1052 LYAFKDLPRFRILICGGDGTVGWVLSCLDDVSTALTYKKPPSAIVPLGTGNDMSRVLQWG 1111



 Score = 41.5 bits (93), Expect = 7e-04
 Identities = 37/125 (29%), Positives = 52/125 (41%), Gaps = 18/125 (14%)

Query: 4   SACKIIAHTACIDI-LMDRVQFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKACG 62
           + C    H AC D  + D  Q      F  +G R  ++     HHW      +G   A  
Sbjct: 717 TVCDYYVHEACQDFSVTDCKQSATYVPF--LGKRAVKQD----HHW-----REGNFSASS 765

Query: 63  KSLQAKLSFSSKEIVA-LSCSWCKDAYHNKESCFNLQRIGEECSLGSQSNITVPPSWIVK 121
           K +  K S  S E +A L CSWC  + H   S    Q + +EC  G    +++PP + V 
Sbjct: 766 KCVSCKHSCGSGECLASLKCSWCAQSSHTNCS----QLLPKECHYGYLHRVSLPP-FCVS 820

Query: 122 LPRRG 126
            P  G
Sbjct: 821 FPDVG 825


>SB_58173| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 163

 Score = 39.1 bits (87), Expect = 0.004
 Identities = 12/18 (66%), Positives = 15/18 (83%)

Query: 46  HHWVHRRSEKGKCKACGK 63
           HHWVHRR + G+C+ CGK
Sbjct: 146 HHWVHRRRQDGRCETCGK 163


>SB_30938| Best HMM Match : DAGK_acc (HMM E-Value=1.4013e-45)
          Length = 709

 Score = 36.3 bits (80), Expect = 0.026
 Identities = 17/31 (54%), Positives = 22/31 (70%), Gaps = 2/31 (6%)

Query: 158 IKPIPTTNVKPVLVFINPKSGGNQGAKLLQK 188
           I P+P T+  P+ VFINPKSGG QG++   K
Sbjct: 339 ITPLPGTH--PLAVFINPKSGGRQGSRATWK 367


>SB_32644| Best HMM Match : zf-C2H2 (HMM E-Value=0)
          Length = 365

 Score = 30.7 bits (66), Expect = 1.3
 Identities = 12/55 (21%), Positives = 25/55 (45%), Gaps = 5/55 (9%)

Query: 42  TVTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIV-----ALSCSWCKDAYHNK 91
           ++  H  +H   +  +CK CGK+ +   + S+  ++        C +C   +H K
Sbjct: 239 SLEQHKSIHTNEKSFECKECGKAFKRSSTLSTHMLIHSDTRPFPCQYCPKRFHQK 293


>SB_45123| Best HMM Match : zf-C2H2 (HMM E-Value=0)
          Length = 336

 Score = 29.5 bits (63), Expect = 3.0
 Identities = 23/103 (22%), Positives = 40/103 (38%), Gaps = 8/103 (7%)

Query: 2   KCSAC-KIIAHTACIDILMDRVQFT-CKPTFRDVGVRQYR-EQTVTHHHWVHRRSEKGKC 58
           +C  C KI      + I +     T  +P   ++  + +R   T+  H  +H + +  KC
Sbjct: 167 RCDQCGKIFKTKYTLSIHLKMPDHTESRPFVCNICGKGFRLSSTLCRHKIIHTQKKPHKC 226

Query: 59  KACGKSLQAKLSFSS-----KEIVALSCSWCKDAYHNKESCFN 96
           + C KS     +  +      E  A  C  C   +H K +  N
Sbjct: 227 EVCNKSFNRSSTLKTHVRTHSEEKAFVCDICGKGFHQKGNLRN 269


>SB_37930| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 707

 Score = 29.1 bits (62), Expect = 4.0
 Identities = 11/26 (42%), Positives = 16/26 (61%)

Query: 39  REQTVTHHHWVHRRSEKGKCKACGKS 64
           R+  +T H  +H+  +   CKACGKS
Sbjct: 657 RKSNLTAHLKMHKNKKPFSCKACGKS 682


>SB_32903| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 765

 Score = 29.1 bits (62), Expect = 4.0
 Identities = 10/26 (38%), Positives = 15/26 (57%)

Query: 38  YREQTVTHHHWVHRRSEKGKCKACGK 63
           YRE  +  H + H + +  KC+ CGK
Sbjct: 491 YREDKLKRHLFTHSKEKPYKCQECGK 516


>SB_45463| Best HMM Match : zf-C2H2 (HMM E-Value=0)
          Length = 347

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 22/87 (25%), Positives = 35/87 (40%), Gaps = 11/87 (12%)

Query: 24  FTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIV-----A 78
           F CK   +  G RQ    T+  H  +H + +  KC  CGK+     + ++   +      
Sbjct: 200 FKCKICGK--GFRQ--ASTLCRHKIIHTKEKPHKCNICGKAFNRSSTLNTHIRIHAGYKP 255

Query: 79  LSCSWCKDAYHNKESCFN--LQRIGEE 103
             C  C   +H K +  N  L   GE+
Sbjct: 256 FQCDICGKGFHQKGNYKNHRLTHTGEK 282



 Score = 27.9 bits (59), Expect = 9.3
 Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 6/59 (10%)

Query: 43  VTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIVAL-----SCSWCKDAYHNKESCFN 96
           +T H  VH  +   KCK CGK  +   +    +I+        C+ C  A+ N+ S  N
Sbjct: 187 LTRHMPVHTGARPFKCKICGKGFRQASTLCRHKIIHTKEKPHKCNICGKAF-NRSSTLN 244


>SB_57| Best HMM Match : zf-C2H2 (HMM E-Value=0)
          Length = 1498

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 13/48 (27%), Positives = 22/48 (45%), Gaps = 6/48 (12%)

Query: 58   CKACGK------SLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQR 99
            C+ C K      S +    + SK+ +   CS C + + N++S    QR
Sbjct: 1032 CQVCDKAFSRVSSYKTHFRYHSKDSLTFKCSHCNETFSNRQSLIKHQR 1079



 Score = 27.9 bits (59), Expect = 9.3
 Identities = 17/81 (20%), Positives = 33/81 (40%), Gaps = 8/81 (9%)

Query: 41   QTVTHHHWVHRRSEKGKCKACGKSLQAK------LSFSSKEIVALSCSWCKDAYHNKESC 94
            + +  H  VH    K  C  C K+ + K          S++I  ++C  C+ ++ ++   
Sbjct: 1327 KALKRHRLVHTSGSKFSCAICNKAFKTKHHMERHSKIHSRDIEGIACPRCEKSFRSEAQL 1386

Query: 95   FNLQRIGEECSLGSQSNITVP 115
               + + E    GS+S    P
Sbjct: 1387 --KRHLSEHKDYGSKSRPRSP 1405


>SB_37978| Best HMM Match : zf-C2H2 (HMM E-Value=0)
          Length = 487

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%)

Query: 23  QFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKACGK 63
           +F C PT     +R Y   T+  H  +H+  ++ KC+ CGK
Sbjct: 290 KFKC-PTCDKAFIRNY---TLQCHMLIHKNVKEHKCETCGK 326


>SB_21417| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 487

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 13/36 (36%), Positives = 17/36 (47%)

Query: 22  VQFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGK 57
           VQ   +P   D   +Q R+  V HHH   +RS   K
Sbjct: 385 VQSNKRPVPNDTRTKQTRKPFVNHHHHSRKRSRTNK 420


>SB_47786| Best HMM Match : Ank (HMM E-Value=4.4e-30)
          Length = 796

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 19/52 (36%), Positives = 24/52 (46%)

Query: 211 ELFRKVPNLRVLACGGDGTVGWVLSVLDRIGSRPAVGVLPLGTGNDLARALG 262
           EL    P   VL     G VG +L  + +I    AVGV+  G   D  R+LG
Sbjct: 146 ELAHTRPGAWVLVHSAAGGVGSMLVQMAKIAGCHAVGVVGAGHKIDFVRSLG 197


>SB_7580| Best HMM Match : Topoisom_bac (HMM E-Value=0)
          Length = 856

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 19/67 (28%), Positives = 25/67 (37%), Gaps = 3/67 (4%)

Query: 44  THHHWVHRRSEKG--KCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQRIG 101
           TH    H     G   C  C K +   L  +S     L+C+ C    H  E  F +    
Sbjct: 708 THPTCPHSEEALGVADCLECEKGM-LNLDPTSAPKWRLACNMCNVVIHMCEGAFRISLSE 766

Query: 102 EECSLGS 108
           +EC  GS
Sbjct: 767 DECDCGS 773


>SB_6589| Best HMM Match : zf-C2H2 (HMM E-Value=1.2e-36)
          Length = 651

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 12/63 (19%), Positives = 30/63 (47%), Gaps = 5/63 (7%)

Query: 36  RQYREQTVTHHHWVHRRSEKGKCKACGKSLQ--AKLSF---SSKEIVALSCSWCKDAYHN 90
           R +++  +  H ++H   +  KCK CG++ +    L +   +  ++    C +C   ++ 
Sbjct: 533 RFHQKADLRIHRYIHTGEKPHKCKKCGRAFKQLTHLKYHMRTHSDVRMYKCPYCDKGFNQ 592

Query: 91  KES 93
           K +
Sbjct: 593 KSN 595


>SB_576| Best HMM Match : RVT_1 (HMM E-Value=0)
          Length = 1444

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 7/54 (12%)

Query: 43  VTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFN 96
           V H +   RR+    C  C K+++     + K  V   C+ CKD  H + +C N
Sbjct: 217 VDHRNKASRRAPAPVCPVCDKAVKC----NQKRYV---CATCKDTTHARWTCSN 263


>SB_9849| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 984

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 18/77 (23%), Positives = 34/77 (44%), Gaps = 4/77 (5%)

Query: 39  REQTVTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQ 98
           RE+  THH  VH+ S + K K  G+    K +  ++E        C  +   +++    +
Sbjct: 439 REEGFTHHSIVHKSSSEEKAKENGE----KSTKENEETAEEKAKECLTSKTEEKAEQQAE 494

Query: 99  RIGEECSLGSQSNITVP 115
             GE+ ++ S+     P
Sbjct: 495 TNGEDAAIKSEGEAKAP 511


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.322    0.138    0.447 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,068,908
Number of Sequences: 59808
Number of extensions: 363414
Number of successful extensions: 909
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 876
Number of HSP's gapped (non-prelim): 38
length of query: 269
length of database: 16,821,457
effective HSP length: 81
effective length of query: 188
effective length of database: 11,977,009
effective search space: 2251677692
effective search space used: 2251677692
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 59 (27.9 bits)

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