BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001172-TA|BGIBMGA001172-PA|IPR001206|Diacylglycerol kinase, catalytic region, IPR002219|Protein kinase C, phorbol ester/diacylglycerol binding (269 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_42132| Best HMM Match : DAGK_cat (HMM E-Value=1.3e-35) 125 3e-29 SB_56638| Best HMM Match : DAGK_cat (HMM E-Value=7.8e-19) 99 2e-21 SB_58879| Best HMM Match : No HMM Matches (HMM E-Value=.) 80 2e-15 SB_52560| Best HMM Match : No HMM Matches (HMM E-Value=.) 71 8e-13 SB_58173| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.004 SB_30938| Best HMM Match : DAGK_acc (HMM E-Value=1.4013e-45) 36 0.026 SB_32644| Best HMM Match : zf-C2H2 (HMM E-Value=0) 31 1.3 SB_45123| Best HMM Match : zf-C2H2 (HMM E-Value=0) 29 3.0 SB_37930| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.0 SB_32903| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.0 SB_45463| Best HMM Match : zf-C2H2 (HMM E-Value=0) 28 7.0 SB_57| Best HMM Match : zf-C2H2 (HMM E-Value=0) 28 7.0 SB_37978| Best HMM Match : zf-C2H2 (HMM E-Value=0) 28 7.0 SB_21417| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.0 SB_47786| Best HMM Match : Ank (HMM E-Value=4.4e-30) 28 9.3 SB_7580| Best HMM Match : Topoisom_bac (HMM E-Value=0) 28 9.3 SB_6589| Best HMM Match : zf-C2H2 (HMM E-Value=1.2e-36) 28 9.3 SB_576| Best HMM Match : RVT_1 (HMM E-Value=0) 28 9.3 SB_9849| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 9.3 >SB_42132| Best HMM Match : DAGK_cat (HMM E-Value=1.3e-35) Length = 558 Score = 125 bits (302), Expect = 3e-29 Identities = 92/271 (33%), Positives = 123/271 (45%), Gaps = 47/271 (17%) Query: 1 MKCSACKIIAHTACIDILMDRVQFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKA 60 + C C + H C++ + FTCK R ++ HH+ C Sbjct: 167 LHCENCTLSVHDECLETANE--VFTCKALVLS------RRSSLKHHYIRGNLPLCSYCSV 218 Query: 61 CGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQRIGEECSLGSQSNITVPPSWIV 120 CG L ++ + C WC++ H + C Q + C G + +PP I Sbjct: 219 CGC-----LCGTAPRLCDQRCIWCQEKVH--DDCLRNQSM--ICDFGRYRTLILPPHCI- 268 Query: 121 KLPRRGXXXXXXXXXXXXXXXXXXXXXXXXNELRTFVIKPIP---TTNVKPVLVFINPKS 177 N+ R FV+K I T N P+LVF N KS Sbjct: 269 ---------------------SLTLVGWRKNKQR-FVVKEITPPNTRNWSPLLVFANCKS 306 Query: 178 GGNQGAKLLQKFQWLLNPRQVFDLTQGGPGPGLELFRKVP--NLRVLACGGDGTVGWVLS 235 G N G +LLQ F+ +LNP QV DL + P LE R +P RVL CGGDG+VGWVL Sbjct: 307 GDNDGERLLQAFRGVLNPVQVIDLHEVPPETALEFCRLLPGHRCRVLVCGGDGSVGWVLD 366 Query: 236 VLDRIGSR--PAVGVLPLGTGNDLARALGWG 264 LD++ + P +G+LPLGTGNDLAR LGWG Sbjct: 367 ALDKVKLKLSPYIGILPLGTGNDLARVLGWG 397 >SB_56638| Best HMM Match : DAGK_cat (HMM E-Value=7.8e-19) Length = 836 Score = 99 bits (238), Expect = 2e-21 Identities = 44/62 (70%), Positives = 52/62 (83%), Gaps = 2/62 (3%) Query: 206 PGPGLELFRKVPNLRVLACGGDGTVGWVLSVLDRI--GSRPAVGVLPLGTGNDLARALGW 263 P P LEL+RKVPN+R+LACGGDGTVGW+LS LD++ RP V +LPLGTGNDL+RAL W Sbjct: 166 PVPTLELYRKVPNVRLLACGGDGTVGWILSELDKLKFNPRPPVAILPLGTGNDLSRALNW 225 Query: 264 GG 265 GG Sbjct: 226 GG 227 >SB_58879| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1786 Score = 80.2 bits (189), Expect = 2e-15 Identities = 42/90 (46%), Positives = 53/90 (58%), Gaps = 19/90 (21%) Query: 168 PVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPG-------------------P 208 P+LVF+N KSG NQG K ++KF+ LLNP QVFDL GGP P Sbjct: 856 PLLVFVNSKSGDNQGVKFIRKFKQLLNPLQVFDLGVGGPTNGVYESFAIHYELINIIFIP 915 Query: 209 GLELFRKVPNLRVLACGGDGTVGWVLSVLD 238 L+ FR+ R+L CGGDG+VGWV+ +D Sbjct: 916 SLKFFRRFRRFRILVCGGDGSVGWVMKEVD 945 Score = 41.9 bits (94), Expect = 5e-04 Identities = 16/19 (84%), Positives = 18/19 (94%) Query: 246 VGVLPLGTGNDLARALGWG 264 +GVLPLGTGNDL+R LGWG Sbjct: 995 IGVLPLGTGNDLSRVLGWG 1013 >SB_52560| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1263 Score = 71.3 bits (167), Expect = 8e-13 Identities = 31/60 (51%), Positives = 41/60 (68%), Gaps = 5/60 (8%) Query: 210 LELFRKVPNLRVLACGGDGTVGWVLSVLDRIGS-----RPAVGVLPLGTGNDLARALGWG 264 L F+ +P R+L CGGDGTVGWVLS LD + + +P ++PLGTGND++R L WG Sbjct: 1052 LYAFKDLPRFRILICGGDGTVGWVLSCLDDVSTALTYKKPPSAIVPLGTGNDMSRVLQWG 1111 Score = 41.5 bits (93), Expect = 7e-04 Identities = 37/125 (29%), Positives = 52/125 (41%), Gaps = 18/125 (14%) Query: 4 SACKIIAHTACIDI-LMDRVQFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKACG 62 + C H AC D + D Q F +G R ++ HHW +G A Sbjct: 717 TVCDYYVHEACQDFSVTDCKQSATYVPF--LGKRAVKQD----HHW-----REGNFSASS 765 Query: 63 KSLQAKLSFSSKEIVA-LSCSWCKDAYHNKESCFNLQRIGEECSLGSQSNITVPPSWIVK 121 K + K S S E +A L CSWC + H S Q + +EC G +++PP + V Sbjct: 766 KCVSCKHSCGSGECLASLKCSWCAQSSHTNCS----QLLPKECHYGYLHRVSLPP-FCVS 820 Query: 122 LPRRG 126 P G Sbjct: 821 FPDVG 825 >SB_58173| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 163 Score = 39.1 bits (87), Expect = 0.004 Identities = 12/18 (66%), Positives = 15/18 (83%) Query: 46 HHWVHRRSEKGKCKACGK 63 HHWVHRR + G+C+ CGK Sbjct: 146 HHWVHRRRQDGRCETCGK 163 >SB_30938| Best HMM Match : DAGK_acc (HMM E-Value=1.4013e-45) Length = 709 Score = 36.3 bits (80), Expect = 0.026 Identities = 17/31 (54%), Positives = 22/31 (70%), Gaps = 2/31 (6%) Query: 158 IKPIPTTNVKPVLVFINPKSGGNQGAKLLQK 188 I P+P T+ P+ VFINPKSGG QG++ K Sbjct: 339 ITPLPGTH--PLAVFINPKSGGRQGSRATWK 367 >SB_32644| Best HMM Match : zf-C2H2 (HMM E-Value=0) Length = 365 Score = 30.7 bits (66), Expect = 1.3 Identities = 12/55 (21%), Positives = 25/55 (45%), Gaps = 5/55 (9%) Query: 42 TVTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIV-----ALSCSWCKDAYHNK 91 ++ H +H + +CK CGK+ + + S+ ++ C +C +H K Sbjct: 239 SLEQHKSIHTNEKSFECKECGKAFKRSSTLSTHMLIHSDTRPFPCQYCPKRFHQK 293 >SB_45123| Best HMM Match : zf-C2H2 (HMM E-Value=0) Length = 336 Score = 29.5 bits (63), Expect = 3.0 Identities = 23/103 (22%), Positives = 40/103 (38%), Gaps = 8/103 (7%) Query: 2 KCSAC-KIIAHTACIDILMDRVQFT-CKPTFRDVGVRQYR-EQTVTHHHWVHRRSEKGKC 58 +C C KI + I + T +P ++ + +R T+ H +H + + KC Sbjct: 167 RCDQCGKIFKTKYTLSIHLKMPDHTESRPFVCNICGKGFRLSSTLCRHKIIHTQKKPHKC 226 Query: 59 KACGKSLQAKLSFSS-----KEIVALSCSWCKDAYHNKESCFN 96 + C KS + + E A C C +H K + N Sbjct: 227 EVCNKSFNRSSTLKTHVRTHSEEKAFVCDICGKGFHQKGNLRN 269 >SB_37930| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 707 Score = 29.1 bits (62), Expect = 4.0 Identities = 11/26 (42%), Positives = 16/26 (61%) Query: 39 REQTVTHHHWVHRRSEKGKCKACGKS 64 R+ +T H +H+ + CKACGKS Sbjct: 657 RKSNLTAHLKMHKNKKPFSCKACGKS 682 >SB_32903| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 765 Score = 29.1 bits (62), Expect = 4.0 Identities = 10/26 (38%), Positives = 15/26 (57%) Query: 38 YREQTVTHHHWVHRRSEKGKCKACGK 63 YRE + H + H + + KC+ CGK Sbjct: 491 YREDKLKRHLFTHSKEKPYKCQECGK 516 >SB_45463| Best HMM Match : zf-C2H2 (HMM E-Value=0) Length = 347 Score = 28.3 bits (60), Expect = 7.0 Identities = 22/87 (25%), Positives = 35/87 (40%), Gaps = 11/87 (12%) Query: 24 FTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIV-----A 78 F CK + G RQ T+ H +H + + KC CGK+ + ++ + Sbjct: 200 FKCKICGK--GFRQ--ASTLCRHKIIHTKEKPHKCNICGKAFNRSSTLNTHIRIHAGYKP 255 Query: 79 LSCSWCKDAYHNKESCFN--LQRIGEE 103 C C +H K + N L GE+ Sbjct: 256 FQCDICGKGFHQKGNYKNHRLTHTGEK 282 Score = 27.9 bits (59), Expect = 9.3 Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 6/59 (10%) Query: 43 VTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIVAL-----SCSWCKDAYHNKESCFN 96 +T H VH + KCK CGK + + +I+ C+ C A+ N+ S N Sbjct: 187 LTRHMPVHTGARPFKCKICGKGFRQASTLCRHKIIHTKEKPHKCNICGKAF-NRSSTLN 244 >SB_57| Best HMM Match : zf-C2H2 (HMM E-Value=0) Length = 1498 Score = 28.3 bits (60), Expect = 7.0 Identities = 13/48 (27%), Positives = 22/48 (45%), Gaps = 6/48 (12%) Query: 58 CKACGK------SLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQR 99 C+ C K S + + SK+ + CS C + + N++S QR Sbjct: 1032 CQVCDKAFSRVSSYKTHFRYHSKDSLTFKCSHCNETFSNRQSLIKHQR 1079 Score = 27.9 bits (59), Expect = 9.3 Identities = 17/81 (20%), Positives = 33/81 (40%), Gaps = 8/81 (9%) Query: 41 QTVTHHHWVHRRSEKGKCKACGKSLQAK------LSFSSKEIVALSCSWCKDAYHNKESC 94 + + H VH K C C K+ + K S++I ++C C+ ++ ++ Sbjct: 1327 KALKRHRLVHTSGSKFSCAICNKAFKTKHHMERHSKIHSRDIEGIACPRCEKSFRSEAQL 1386 Query: 95 FNLQRIGEECSLGSQSNITVP 115 + + E GS+S P Sbjct: 1387 --KRHLSEHKDYGSKSRPRSP 1405 >SB_37978| Best HMM Match : zf-C2H2 (HMM E-Value=0) Length = 487 Score = 28.3 bits (60), Expect = 7.0 Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 23 QFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGKCKACGK 63 +F C PT +R Y T+ H +H+ ++ KC+ CGK Sbjct: 290 KFKC-PTCDKAFIRNY---TLQCHMLIHKNVKEHKCETCGK 326 >SB_21417| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 487 Score = 28.3 bits (60), Expect = 7.0 Identities = 13/36 (36%), Positives = 17/36 (47%) Query: 22 VQFTCKPTFRDVGVRQYREQTVTHHHWVHRRSEKGK 57 VQ +P D +Q R+ V HHH +RS K Sbjct: 385 VQSNKRPVPNDTRTKQTRKPFVNHHHHSRKRSRTNK 420 >SB_47786| Best HMM Match : Ank (HMM E-Value=4.4e-30) Length = 796 Score = 27.9 bits (59), Expect = 9.3 Identities = 19/52 (36%), Positives = 24/52 (46%) Query: 211 ELFRKVPNLRVLACGGDGTVGWVLSVLDRIGSRPAVGVLPLGTGNDLARALG 262 EL P VL G VG +L + +I AVGV+ G D R+LG Sbjct: 146 ELAHTRPGAWVLVHSAAGGVGSMLVQMAKIAGCHAVGVVGAGHKIDFVRSLG 197 >SB_7580| Best HMM Match : Topoisom_bac (HMM E-Value=0) Length = 856 Score = 27.9 bits (59), Expect = 9.3 Identities = 19/67 (28%), Positives = 25/67 (37%), Gaps = 3/67 (4%) Query: 44 THHHWVHRRSEKG--KCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQRIG 101 TH H G C C K + L +S L+C+ C H E F + Sbjct: 708 THPTCPHSEEALGVADCLECEKGM-LNLDPTSAPKWRLACNMCNVVIHMCEGAFRISLSE 766 Query: 102 EECSLGS 108 +EC GS Sbjct: 767 DECDCGS 773 >SB_6589| Best HMM Match : zf-C2H2 (HMM E-Value=1.2e-36) Length = 651 Score = 27.9 bits (59), Expect = 9.3 Identities = 12/63 (19%), Positives = 30/63 (47%), Gaps = 5/63 (7%) Query: 36 RQYREQTVTHHHWVHRRSEKGKCKACGKSLQ--AKLSF---SSKEIVALSCSWCKDAYHN 90 R +++ + H ++H + KCK CG++ + L + + ++ C +C ++ Sbjct: 533 RFHQKADLRIHRYIHTGEKPHKCKKCGRAFKQLTHLKYHMRTHSDVRMYKCPYCDKGFNQ 592 Query: 91 KES 93 K + Sbjct: 593 KSN 595 >SB_576| Best HMM Match : RVT_1 (HMM E-Value=0) Length = 1444 Score = 27.9 bits (59), Expect = 9.3 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 7/54 (12%) Query: 43 VTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFN 96 V H + RR+ C C K+++ + K V C+ CKD H + +C N Sbjct: 217 VDHRNKASRRAPAPVCPVCDKAVKC----NQKRYV---CATCKDTTHARWTCSN 263 >SB_9849| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 984 Score = 27.9 bits (59), Expect = 9.3 Identities = 18/77 (23%), Positives = 34/77 (44%), Gaps = 4/77 (5%) Query: 39 REQTVTHHHWVHRRSEKGKCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKESCFNLQ 98 RE+ THH VH+ S + K K G+ K + ++E C + +++ + Sbjct: 439 REEGFTHHSIVHKSSSEEKAKENGE----KSTKENEETAEEKAKECLTSKTEEKAEQQAE 494 Query: 99 RIGEECSLGSQSNITVP 115 GE+ ++ S+ P Sbjct: 495 TNGEDAAIKSEGEAKAP 511 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.322 0.138 0.447 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,068,908 Number of Sequences: 59808 Number of extensions: 363414 Number of successful extensions: 909 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 876 Number of HSP's gapped (non-prelim): 38 length of query: 269 length of database: 16,821,457 effective HSP length: 81 effective length of query: 188 effective length of database: 11,977,009 effective search space: 2251677692 effective search space used: 2251677692 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 59 (27.9 bits)
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