BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001172-TA|BGIBMGA001172-PA|IPR001206|Diacylglycerol kinase, catalytic region, IPR002219|Protein kinase C, phorbol ester/diacylglycerol binding (269 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g07920.1 68418.m00916 diacylglycerol kinase 1 (DGK1) identica... 121 4e-28 At5g63770.1 68418.m08004 diacylglycerol kinase, putative similar... 113 1e-25 At2g18730.1 68415.m02181 diacylglycerol kinase, putative contain... 94 8e-20 At5g57690.1 68418.m07211 diacylglycerol kinase, putative contain... 91 7e-19 At4g30340.1 68417.m04312 diacylglycerol kinase family protein co... 90 2e-18 At4g28130.1 68417.m04033 diacylglycerol kinase accessory domain-... 84 8e-17 At2g20900.3 68415.m02465 diacylglycerol kinase, putative contain... 83 2e-16 At2g20900.2 68415.m02464 diacylglycerol kinase, putative contain... 83 2e-16 At2g20900.1 68415.m02463 diacylglycerol kinase, putative contain... 83 2e-16 At2g46090.1 68415.m05733 diacylglycerol kinase family protein co... 44 1e-04 At4g36810.1 68417.m05221 geranylgeranyl pyrophosphate synthase (... 29 2.5 At3g11080.1 68416.m01339 disease resistance family protein conta... 29 4.3 At5g58610.1 68418.m07345 PHD finger transcription factor, putative 28 5.7 >At5g07920.1 68418.m00916 diacylglycerol kinase 1 (DGK1) identical to diacylglycerol kinase 1 (Diglyceride kinase 1, DGK 1, DAG kinase 1) [Arabidopsis thaliana] SWISS-PROT:Q39017 Length = 728 Score = 121 bits (292), Expect = 4e-28 Identities = 59/105 (56%), Positives = 73/105 (69%), Gaps = 3/105 (2%) Query: 164 TNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQ-GGPGPGLELFRKVPNLRVL 222 ++ +P+LVFIN KSG +G L Q+ LNP QVF+L+ GP GL LFRKVP+ RVL Sbjct: 357 SDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVL 416 Query: 223 ACGGDGTVGWVLSVLDRIG--SRPAVGVLPLGTGNDLARALGWGG 265 CGGDGT GWVL +++ S PAV +LP GTGNDL+R L WGG Sbjct: 417 VCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGG 461 >At5g63770.1 68418.m08004 diacylglycerol kinase, putative similar to diacylglycerol kinase, theta (diglyceride kinase, DGK- theta, DAG kinase theta). [Homo sapiens] SWISS-PROT:P52824 Length = 712 Score = 113 bits (272), Expect = 1e-25 Identities = 60/122 (49%), Positives = 76/122 (62%), Gaps = 4/122 (3%) Query: 151 NELRTFVIKPIPTTNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQ-GGPGPG 209 N ++ F + +P + +P+LVFIN KSGG G L ++ LLNP QVF+L GP G Sbjct: 326 NFMKKFSLVDLPP-DARPLLVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQGPDAG 384 Query: 210 LELFRKVPNLRVLACGGDGTVGWVLSVLDR--IGSRPAVGVLPLGTGNDLARALGWGGAT 267 L+L KV RVL CGGDGTV WVL +++ S P V +LPLGTGNDL+R L WG Sbjct: 385 LDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQWGRGI 444 Query: 268 DV 269 V Sbjct: 445 SV 446 Score = 27.9 bits (59), Expect = 7.6 Identities = 11/36 (30%), Positives = 16/36 (44%) Query: 81 CSWCKDAYHNKESCFNLQRIGEECSLGSQSNITVPP 116 C WC+ H K + G+ C LGS + + P Sbjct: 185 CLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSP 220 >At2g18730.1 68415.m02181 diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 488 Score = 94.3 bits (224), Expect = 8e-20 Identities = 54/120 (45%), Positives = 68/120 (56%), Gaps = 21/120 (17%) Query: 168 PVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPGP----GLELFRKVP------ 217 P++VFINP SGG G L ++ Q L++ QVFDLT+ P GL KV Sbjct: 91 PMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDEC 150 Query: 218 ------NLRVLACGGDGTVGWVLSVLDRIGSR-----PAVGVLPLGTGNDLARALGWGGA 266 LR++ GGDGTVGWVL L + P VGV+PLGTGNDL+R+ GWGG+ Sbjct: 151 AKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGS 210 >At5g57690.1 68418.m07211 diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 498 Score = 91.1 bits (216), Expect = 7e-19 Identities = 51/120 (42%), Positives = 74/120 (61%), Gaps = 21/120 (17%) Query: 168 PVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGP------GPG-LELF------- 213 P++VF+NPKSGG QG + ++ Q L++ QV+DLT+ P G G LE F Sbjct: 90 PLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDEC 149 Query: 214 -RKV-PNLRVLACGGDGTVGWVLSVLDRIGSR-----PAVGVLPLGTGNDLARALGWGGA 266 +++ +R++ GGDGTVGWVL L + + P V ++PLGTGNDL+R+ GWGG+ Sbjct: 150 AKEIREKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGS 209 >At4g30340.1 68417.m04312 diacylglycerol kinase family protein contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 374 Score = 89.8 bits (213), Expect = 2e-18 Identities = 52/120 (43%), Positives = 67/120 (55%), Gaps = 21/120 (17%) Query: 168 PVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGP---------------GPGLEL 212 P++VFINPKSGG G L ++ Q L+ QVFDLT+ P G E Sbjct: 94 PMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDEC 153 Query: 213 FRKV-PNLRVLACGGDGTVGWVLSVLDRIGSR-----PAVGVLPLGTGNDLARALGWGGA 266 R+ +R++ GGDGTVGWVL L + P VGV+PLGTGNDL+R+ WGG+ Sbjct: 154 ARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGS 213 >At4g28130.1 68417.m04033 diacylglycerol kinase accessory domain-containing protein similar to diacylglycerol kinase [Lycopersicon esculentum] GI:10798892; contains Pfam profile PF00609: Diacylglycerol kinase accessory domain (presumed) Length = 466 Score = 84.2 bits (199), Expect = 8e-17 Identities = 48/118 (40%), Positives = 66/118 (55%), Gaps = 16/118 (13%) Query: 163 TTNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPGPGLE----------- 211 T P+LVFIN KSGG GA+L+ ++ LLN +QVFDL P L+ Sbjct: 42 TAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLKD 101 Query: 212 --LFRKV-PNLRVLACGGDGTVGWVLSVLD--RIGSRPAVGVLPLGTGNDLARALGWG 264 L K+ L+++ GGDGT GW+L V+ + + P + +PLGTGN+L A GWG Sbjct: 102 DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPPPIATVPLGTGNNLPFAFGWG 159 >At2g20900.3 68415.m02465 diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 491 Score = 83.0 bits (196), Expect = 2e-16 Identities = 48/117 (41%), Positives = 65/117 (55%), Gaps = 16/117 (13%) Query: 164 TNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPGPGL------------- 210 T PVLVFIN KSGG G +L+ ++ LLN QVFDL Q P L Sbjct: 36 TPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKDD 95 Query: 211 ELFRKV-PNLRVLACGGDGTVGWVLSVLD--RIGSRPAVGVLPLGTGNDLARALGWG 264 + R++ L+++ GGDGT GW+L V+ ++ P + +PLGTGN+L A GWG Sbjct: 96 DFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLSHPPPIATVPLGTGNNLPFAFGWG 152 >At2g20900.2 68415.m02464 diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 491 Score = 83.0 bits (196), Expect = 2e-16 Identities = 48/117 (41%), Positives = 65/117 (55%), Gaps = 16/117 (13%) Query: 164 TNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPGPGL------------- 210 T PVLVFIN KSGG G +L+ ++ LLN QVFDL Q P L Sbjct: 36 TPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKDD 95 Query: 211 ELFRKV-PNLRVLACGGDGTVGWVLSVLD--RIGSRPAVGVLPLGTGNDLARALGWG 264 + R++ L+++ GGDGT GW+L V+ ++ P + +PLGTGN+L A GWG Sbjct: 96 DFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLSHPPPIATVPLGTGNNLPFAFGWG 152 >At2g20900.1 68415.m02463 diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 509 Score = 83.0 bits (196), Expect = 2e-16 Identities = 48/117 (41%), Positives = 65/117 (55%), Gaps = 16/117 (13%) Query: 164 TNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPGPGL------------- 210 T PVLVFIN KSGG G +L+ ++ LLN QVFDL Q P L Sbjct: 36 TPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKDD 95 Query: 211 ELFRKV-PNLRVLACGGDGTVGWVLSVLD--RIGSRPAVGVLPLGTGNDLARALGWG 264 + R++ L+++ GGDGT GW+L V+ ++ P + +PLGTGN+L A GWG Sbjct: 96 DFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLSHPPPIATVPLGTGNNLPFAFGWG 152 >At2g46090.1 68415.m05733 diacylglycerol kinase family protein contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain Length = 364 Score = 43.6 bits (98), Expect = 1e-04 Identities = 35/122 (28%), Positives = 58/122 (47%), Gaps = 19/122 (15%) Query: 161 IPTTNVKPVLVFINPKSGGNQGAKLLQKFQWLLNPR-----QVFDLTQGGPGPGLELFRK 215 + ++ ++ ++ +NP+ + AK +K L R V +L GP +++ R+ Sbjct: 46 VSSSGLRDLVFVVNPQGANGRTAKEWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITRE 105 Query: 216 VPNL---RVLACGGDGTV----------GWVLSVLDRIGSRP-AVGVLPLGTGNDLARAL 261 V+A GGDGT+ G + L SR A+G++PLGTG+D AR Sbjct: 106 AIRDGADAVIAVGGDGTLHEVVNGFFWEGKPVGYLSGEASRSTALGLIPLGTGSDFARTF 165 Query: 262 GW 263 GW Sbjct: 166 GW 167 >At4g36810.1 68417.m05221 geranylgeranyl pyrophosphate synthase (GGPS1) / GGPP synthetase / farnesyltranstransferase identical to GI:413730 to geranylgeranyl pyrophosphate synthase, chloroplast precursor GB:P34802 from [Arabidopsis thaliana] synonymous with farnesyltranstransferase Length = 371 Score = 29.5 bits (63), Expect = 2.5 Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 3/53 (5%) Query: 43 VTHHHWVHRRSE---KGKCKACGKSLQAKLSFSSKEIVALSCSWCKDAYHNKE 92 V HHH H S K + ++C +L +SF SK V+ S S + KE Sbjct: 11 VVHHHNHHHPSSILTKSRSRSCPITLTKPISFRSKRTVSSSSSIVSSSVVTKE 63 >At3g11080.1 68416.m01339 disease resistance family protein contains leucine rich-repeat domains Pfam:PF00560, INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon pimpinellifolium] gi|1184077|gb|AAC15780 Length = 943 Score = 28.7 bits (61), Expect = 4.3 Identities = 10/22 (45%), Positives = 14/22 (63%) Query: 79 LSCSWCKDAYHNKESCFNLQRI 100 LSCSW ++H+ S F LQ + Sbjct: 106 LSCSWLYGSFHSNSSLFRLQNL 127 >At5g58610.1 68418.m07345 PHD finger transcription factor, putative Length = 1065 Score = 28.3 bits (60), Expect = 5.7 Identities = 12/32 (37%), Positives = 17/32 (53%) Query: 58 CKACGKSLQAKLSFSSKEIVALSCSWCKDAYH 89 C ACG+ S ++KE +SC C+ YH Sbjct: 738 CGACGQFFLKTTSTNAKEEKFISCKQCELKYH 769 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.322 0.138 0.447 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,426,535 Number of Sequences: 28952 Number of extensions: 263657 Number of successful extensions: 591 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 560 Number of HSP's gapped (non-prelim): 20 length of query: 269 length of database: 12,070,560 effective HSP length: 80 effective length of query: 189 effective length of database: 9,754,400 effective search space: 1843581600 effective search space used: 1843581600 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 59 (27.9 bits)
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