BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001163-TA|BGIBMGA001163-PA|IPR002290|Serine/threonine protein kinase, IPR000961|Protein kinase, C-terminal, IPR000719|Protein kinase, IPR008271|Serine/threonine protein kinase, active site, IPR011009|Protein kinase-like (366 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 251 8e-69 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 111 7e-27 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 97 2e-22 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 73 5e-15 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 73 5e-15 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 64 2e-12 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 59 7e-11 AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein. 23 5.5 DQ494419-1|ABF55370.1| 127|Apis mellifera telomerase reverse tr... 22 9.6 DQ494418-1|ABF55369.1| 110|Apis mellifera telomerase reverse tr... 22 9.6 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 251 bits (614), Expect = 8e-69 Identities = 127/326 (38%), Positives = 193/326 (59%), Gaps = 13/326 (3%) Query: 43 VESWDDKS---ESNAKLNDFERQKV-----LGTGAFGIVFLVKHIV-TGKYYAMKMLEKE 93 VE W++++ E N + D Q + LG G FG V LV+ + + +A+K ++K Sbjct: 343 VEGWENRATIPELNEEFRDLRLQDLRPLATLGVGGFGRVELVQIAGDSSRSFALKQMKKA 402 Query: 94 KIVRLKQIEHSYYEKKILCGLNFPFVVYMKYYFKDNVYLYYVLPFIAGGEMFSHLRKMGK 153 +IV +Q +H EK+I+ + FVV + FKD YLY ++ GGE+++ LR G Sbjct: 403 QIVETRQQQHIMSEKRIMGEADCDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGH 462 Query: 154 FEETSAKFYGAQVILALEYLHACELVYRDLKPENILIDRTGYIKITDFGFCK-LIRGR-T 211 F++ + +FY A V+ A +YLH+ ++YRDLKPEN+L+D GY+K+ DFGF K L GR T Sbjct: 463 FDDGTTRFYTACVVEAFDYLHSRNIIYRDLKPENLLLDSQGYVKLVDFGFAKRLDHGRKT 522 Query: 212 WTLCGTPEYLAPEIILSKGYGMSVDWWSLGVLLFEMSAGHPPFFASDPMRIYEKIVAG-- 269 WT CGTPEY+APE+IL+KG+ +S D+WSLGVL+FE+ G PPF DPM+ Y I+ G Sbjct: 523 WTFCGTPEYVAPEVILNKGHDISADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILKGID 582 Query: 270 KYRCPSHFSVELKDIIGHVLQVDTTRRYGTLKDGVLDFKNHKWFREIDWDSILNSRIHPP 329 P + +I + + + R G K G+ + + HKWF +W+ + + PP Sbjct: 583 AIEFPRSITRNATALIKKLCRDNPAERLGYQKGGISEIQKHKWFDGFNWEGLRARTLEPP 642 Query: 330 FFPKIRSPGDTTNFDVFDEEKIKDSP 355 P++++ DTTNFD + + P Sbjct: 643 IMPRVQNATDTTNFDEYPPDSDPPPP 668 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 111 bits (268), Expect = 7e-27 Identities = 56/142 (39%), Positives = 91/142 (64%), Gaps = 4/142 (2%) Query: 81 TGKYYAMKMLEKEKIVRLKQIEHSYYEKKILC-GLNFPFVVYMKYYFKDNVYLYYVLPFI 139 T + YA+K+L+K+ I++ +E + EK++L PF+V + F+ LY+V+ ++ Sbjct: 8 TDELYAIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLYFVMEYV 67 Query: 140 AGGEMFSHLRKMGKFEETSAKFYGAQVILALEYLHACELVYRDLKPENILIDRTGYIKIT 199 GG++ +++ GKF+E A FY +++ + L +LH +VYRDLK +N+L+D+ G+IKI Sbjct: 68 NGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQDGHIKIA 127 Query: 200 DFGFCKL-IRG--RTWTLCGTP 218 DFG CK I G T T CGTP Sbjct: 128 DFGMCKEGISGDKTTKTFCGTP 149 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 97.1 bits (231), Expect = 2e-22 Identities = 52/153 (33%), Positives = 87/153 (56%), Gaps = 10/153 (6%) Query: 154 FEETSAKFYGAQVILALEYLHACELVYRDLKPENILID---RTGYIKITDFGFCKLIRG- 209 + E A Q++ ++ + H +V+RDLKPEN+L+ + +K+ DFG ++G Sbjct: 6 YSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGE 65 Query: 210 -RTWT-LCGTPEYLAPEIILSKGYGMSVDWWSLGVLLFEMSAGHPPFFASDPMRIYEKIV 267 + W GTP YL+PE++ + YG VD W+ GV+L+ + G+PPF+ D R+Y +I Sbjct: 66 AQAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIK 125 Query: 268 AGKYRCPS----HFSVELKDIIGHVLQVDTTRR 296 G Y PS + E K++I +L V+ ++R Sbjct: 126 TGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKR 158 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 72.5 bits (170), Expect = 5e-15 Identities = 36/86 (41%), Positives = 55/86 (63%), Gaps = 1/86 (1%) Query: 166 VILALEYLHACELVYRDLKPENILIDRTGYIKITDFGFCKLIRGRTWTLCGTPEYLAPEI 225 V+ + YLH+ LV+RD+K +N+L+D K+TDFGFC ++ GTP ++APE Sbjct: 706 VLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMAPE- 764 Query: 226 ILSKGYGMSVDWWSLGVLLFEMSAGH 251 +LS Y SVD ++ G+L + + AGH Sbjct: 765 LLSGHYDSSVDVYAFGILFWYLCAGH 790 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 72.5 bits (170), Expect = 5e-15 Identities = 36/86 (41%), Positives = 55/86 (63%), Gaps = 1/86 (1%) Query: 166 VILALEYLHACELVYRDLKPENILIDRTGYIKITDFGFCKLIRGRTWTLCGTPEYLAPEI 225 V+ + YLH+ LV+RD+K +N+L+D K+TDFGFC ++ GTP ++APE Sbjct: 744 VLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFCITEVMMLGSIVGTPVHMAPE- 802 Query: 226 ILSKGYGMSVDWWSLGVLLFEMSAGH 251 +LS Y SVD ++ G+L + + AGH Sbjct: 803 LLSGHYDSSVDVYAFGILFWYLCAGH 828 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 63.7 bits (148), Expect = 2e-12 Identities = 54/193 (27%), Positives = 91/193 (47%), Gaps = 14/193 (7%) Query: 65 LGTGAFGIVFLVKHIVTGKYYAMKMLEKEKIVRLKQIE-HSYYEKKILCGLNFPFVVYMK 123 LG+G FGIV+ K + G+ A K+++ EK + E H+ + K N V+ ++ Sbjct: 73 LGSGGFGIVY--KALYKGEQVAAKIIQTEKYSNMLNSEKHASFLKHS----NIVKVLMIE 126 Query: 124 YYFKDNVYLYYVLPFIAGGEMFSHLRKMGKFEETSAKFYGAQVILALEYLHACELVYRDL 183 L + + G + + L + + + + AL++ H +V+ D+ Sbjct: 127 ----QGASLSLITMELCGTTLQNRLDEAILIKNERICILKS-ITCALQFCHNAGIVHADV 181 Query: 184 KPENILIDRTGYIKITDFGFCKLI--RGRTWTLCGTPEYLAPEIILSKGYGMSVDWWSLG 241 KP+NIL+ + G K+TDFG LI GTP Y APE+I + D +SLG Sbjct: 182 KPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAPEVIKQNRPTPAADIYSLG 241 Query: 242 VLLFEMSAGHPPF 254 ++ ++M PF Sbjct: 242 IVAWQMLFRKLPF 254 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 58.8 bits (136), Expect = 7e-11 Identities = 46/177 (25%), Positives = 80/177 (45%), Gaps = 9/177 (5%) Query: 107 EKKILCGLNFPFVVYMKYYFKDNVYLYYVLPFIAGGEMFSHLRKM-GKFEETSAKFYGAQ 165 E I+ P V++++ + + + F+ G + + LR GKF+ Sbjct: 684 EASIMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRG 743 Query: 166 VILALEYLHACELVYRDLKPENILIDRTGYIKITDFGFCKLIRGRT---WTLCG---TPE 219 + ++YL V+RDL N+L++ KI DFG + I T +T G Sbjct: 744 IASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKIPVR 803 Query: 220 YLAPEIILSKGYGMSVDWWSLGVLLFE-MSAGHPPFFASDPMRIYEKIVAGKYRCPS 275 + APE I + + + D WS+G++ +E MS G P++ + + I G YR P+ Sbjct: 804 WTAPEAIAFRKFTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKG-YRLPA 859 >AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein. Length = 429 Score = 22.6 bits (46), Expect = 5.5 Identities = 13/42 (30%), Positives = 21/42 (50%), Gaps = 3/42 (7%) Query: 117 PFVVYMKYYFKDNVYLYYVLPFIAGGEMFSHLRKMGKFEETS 158 P +V F D L+ ++ FI GE+ H R + F +T+ Sbjct: 69 PVIVLQDVAFSD---LHALVEFIYHGEVNVHQRSLSSFLKTA 107 >DQ494419-1|ABF55370.1| 127|Apis mellifera telomerase reverse transcriptase protein. Length = 127 Score = 21.8 bits (44), Expect = 9.6 Identities = 8/27 (29%), Positives = 14/27 (51%) Query: 114 LNFPFVVYMKYYFKDNVYLYYVLPFIA 140 L+ + Y K + KDN+ Y + F + Sbjct: 101 LHILWTSYKKVFMKDNILRQYFITFFS 127 >DQ494418-1|ABF55369.1| 110|Apis mellifera telomerase reverse transcriptase protein. Length = 110 Score = 21.8 bits (44), Expect = 9.6 Identities = 8/27 (29%), Positives = 14/27 (51%) Query: 114 LNFPFVVYMKYYFKDNVYLYYVLPFIA 140 L+ + Y K + KDN+ Y + F + Sbjct: 84 LHILWTSYKKVFMKDNILRQYFITFFS 110 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.323 0.142 0.442 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 115,394 Number of Sequences: 429 Number of extensions: 5273 Number of successful extensions: 27 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 6 Number of HSP's gapped (non-prelim): 11 length of query: 366 length of database: 140,377 effective HSP length: 59 effective length of query: 307 effective length of database: 115,066 effective search space: 35325262 effective search space used: 35325262 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 44 (21.8 bits)
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