Silkworm BLAST Result against bee

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001162-TA|BGIBMGA001162-PA|IPR002401|E-class P450, group
I, IPR001128|Cytochrome P450
         (556 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ244075-1|ABB36785.1|  548|Apis mellifera cytochrome P450 monoo...   784   0.0  
DQ232888-1|ABB36783.1|  499|Apis mellifera cytochrome P450 monoo...   132   1e-32
DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450 monoo...    77   4e-16

>DQ244075-1|ABB36785.1|  548|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 548

 Score =  784 bits (1938), Expect = 0.0
 Identities = 367/541 (67%), Positives = 454/541 (83%), Gaps = 5/541 (0%)

Query: 15  FSAINLFYVLLVPAVILWYAYWRMSRRRLYELADKLNXXXXXXXXXNALEFVGGSADIFR 74
           FSA ++F  LL+PA+IL++ Y+R+SRR L ELA+K+          NAL+  G    +F 
Sbjct: 9   FSASSVFLSLLIPALILYFIYFRISRRHLLELAEKIPGPPALPLIGNALDLFGSPDAMFS 68

Query: 75  NIVQKSADYDHESVVKIWIGPRLLVFLYDPRDVEVILSSHVYIDKAEEYRFFKPWLGNGL 134
            +++K+ ++  + VVKIW+GP+L++ L DPRDVE+ILSS+VYIDK+ EYRFFKPWLG+GL
Sbjct: 69  QVLKKAENF--KDVVKIWVGPKLVICLIDPRDVEIILSSNVYIDKSTEYRFFKPWLGDGL 126

Query: 135 LISTGQKWRSHRKLIAPTFHLNVLKSFIDLFNANSRAVVDKLKKE-ASNFDCHDYMSECT 193
           LISTGQKWR+HRKLIAPTFHLNVLKSFIDLFNAN+R+VV+K++KE    FDCH+YMSE T
Sbjct: 127 LISTGQKWRNHRKLIAPTFHLNVLKSFIDLFNANARSVVEKMRKENGKEFDCHNYMSELT 186

Query: 194 VEILLETAMGVSKSTQDQSGFEYAMAVMKMCDILHLRHTKIWLRPDLLFKFTDYAKNQTK 253
           V+ILLETAMGVSK T+D + FEYAMAVMKMCDILHLRHTKIWLRPD LF  T Y KNQ K
Sbjct: 187 VDILLETAMGVSKPTRDHNAFEYAMAVMKMCDILHLRHTKIWLRPDWLFNLTKYGKNQIK 246

Query: 254 LLDIIHGLTKKVIKRKKEEFASGKKPSNLNETATTSEPSTGKLTSVEGLSFGQSSGLKXX 313
           LL+IIHGLTKKVI+ KKEE+ SGK+ + ++ +A  +E  T  +  VEG+SFGQS GLK  
Sbjct: 247 LLEIIHGLTKKVIQLKKEEYKSGKR-NIIDNSAQKTESKTNNIV-VEGVSFGQSVGLKDD 304

Query: 314 XXXXXXXGQKKRLAFLDLLLESSQSGVAISDEEIKEQVDTIMFEGHDTTAAGSSFFLSMM 373
                  G+KKR AFLDLL+E+ Q+GV ++D+E+KEQVDTIMFEGHDTTA+GSSFFL++M
Sbjct: 305 LDIDDDVGEKKRQAFLDLLIEAGQNGVLLTDKEVKEQVDTIMFEGHDTTASGSSFFLAVM 364

Query: 374 GIHQDIQDKVIEELDQIFGDSDRPVTFQDTLEMKYLERCLMETLRLYPPVPIIARQVNQE 433
           G H DIQ+KVI+ELD+IFGDSDRP TFQDTLEMKYLERCL+ETLR+YPPVP+IAR++  +
Sbjct: 365 GCHPDIQEKVIQELDEIFGDSDRPATFQDTLEMKYLERCLLETLRMYPPVPLIAREIKTD 424

Query: 434 ITLPSNGKKIPAGTTLVIATYKLHRRPDVYPNPNKFDPDNFLPERSANRHYYAFVPFSAG 493
           + L S    IPAG T+VI T+KLHR+P +YPNP+ FDPDNFLPE++ANRHYYAFVPFSAG
Sbjct: 425 LKLASGDYTIPAGCTVVIGTFKLHRQPHIYPNPDVFDPDNFLPEKTANRHYYAFVPFSAG 484

Query: 494 PRSCVGRKYAMLKLKVILSTILRNFRVISDLKESDFKLQADIILKRAEGFQVRLQPRKRM 553
           PRSCVGRKYAMLKLK++LSTILRNFRV SD+KES+F+LQADIILKRA+GF++RL+PRK++
Sbjct: 485 PRSCVGRKYAMLKLKIVLSTILRNFRVRSDVKESEFRLQADIILKRADGFKIRLEPRKQV 544

Query: 554 A 554
           A
Sbjct: 545 A 545


>DQ232888-1|ABB36783.1|  499|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 499

 Score =  132 bits (318), Expect = 1e-32
 Identities = 64/198 (32%), Positives = 112/198 (56%), Gaps = 5/198 (2%)

Query: 328 FLDLLLESSQS-----GVAISDEEIKEQVDTIMFEGHDTTAAGSSFFLSMMGIHQDIQDK 382
           F+++L+E  ++      + ++D  I  Q       G +T++   S  L  + ++QD+Q K
Sbjct: 270 FINMLMELQKNPQKLENIKLTDSLIAAQAFVFFLAGFETSSTTMSNALYELALNQDVQKK 329

Query: 383 VIEELDQIFGDSDRPVTFQDTLEMKYLERCLMETLRLYPPVPIIARQVNQEITLPSNGKK 442
           + EE++     +++ + + D  EM+YL++   ETLR+YPP  I+ R+   + T       
Sbjct: 330 LREEINTFCPKNNKELKYDDIKEMEYLDKVFKETLRMYPPASILMRKAISDYTFNDTKIT 389

Query: 443 IPAGTTLVIATYKLHRRPDVYPNPNKFDPDNFLPERSANRHYYAFVPFSAGPRSCVGRKY 502
           IP    + I  + +HR   +YPNP+ FDP+ F  +  A+RH   ++PF  GPR+C+G ++
Sbjct: 390 IPKEMKIWIPAFAIHRDSAIYPNPDSFDPERFDQDAMASRHPMHYLPFGDGPRNCIGARF 449

Query: 503 AMLKLKVILSTILRNFRV 520
           A+ + KV L TILRN +V
Sbjct: 450 AVYQTKVGLITILRNHKV 467



 Score = 34.7 bits (76), Expect = 0.002
 Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 3/80 (3%)

Query: 140 QKWRSHRKLIAPTFHLNVLKSFIDLF---NANSRAVVDKLKKEASNFDCHDYMSECTVEI 196
           ++WR  R  ++P F    LK    L    + N    +DKL ++    +C +  +  T ++
Sbjct: 126 ERWRPLRSRLSPIFTSGKLKEMFYLIIECSLNLETYLDKLIEKNEPIECRELTARFTTDV 185

Query: 197 LLETAMGVSKSTQDQSGFEY 216
           +   A G+  S+      E+
Sbjct: 186 IGSCAFGIDMSSMTNENSEF 205


>DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 517

 Score = 77.0 bits (181), Expect = 4e-16
 Identities = 48/170 (28%), Positives = 89/170 (52%), Gaps = 7/170 (4%)

Query: 368 FFLSMMGIHQDIQDKVIEELDQIFGDSDRPVTFQDTLEMKYLERCLMETLRLYPPVPIIA 427
           F   ++G +  +Q+K+ EE   +   +   +T  +  + KYL  C+ E+LRL P    IA
Sbjct: 341 FLFDLIGRNPTVQNKLYEETYAL-APAGCDLTIDNLRKAKYLRACITESLRLIPTTTCIA 399

Query: 428 RQVNQEITLPSNGKKIPAGTTLVIATYKLHRRPDVYPNPNKFDPDNFLPERSANRHYYAF 487
           R +++ I L  +G ++ AGT +++ T+      + + +  K+ P+ +    + +      
Sbjct: 400 RILDEPIEL--SGYRLTAGTVVLLHTWIAGLNEENFKDAKKYLPERWTTPTTPHSPLLV- 456

Query: 488 VPFSAGPRSCVGRKYAMLKLKVILSTILRNFRVISDLKESDFKLQADIIL 537
            PF AG R C G+++  L L++IL+ I+R F +I    E +  LQ + IL
Sbjct: 457 APFGAGRRICPGKRFVDLALQLILAKIIREFEII---VEEELDLQFEFIL 503


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.321    0.136    0.397 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 154,038
Number of Sequences: 429
Number of extensions: 6744
Number of successful extensions: 18
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 10
Number of HSP's gapped (non-prelim): 4
length of query: 556
length of database: 140,377
effective HSP length: 61
effective length of query: 495
effective length of database: 114,208
effective search space: 56532960
effective search space used: 56532960
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 46 (22.6 bits)