BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001160-TA|BGIBMGA001160-PA|IPR003010|Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase, IPR003694|NAD+ synthase (697 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|... 830 0.0 SPAC30C2.08 |||conserved fungal protein|Schizosaccharomyces pomb... 30 0.94 SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex ... 30 0.94 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 28 5.0 SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 28 5.0 >SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|chr 3|||Manual Length = 700 Score = 830 bits (2052), Expect = 0.0 Identities = 393/691 (56%), Positives = 492/691 (71%), Gaps = 6/691 (0%) Query: 1 MGRKVTVAVCTLNQWALDFEGNLNRILQSIQEAKELGALYRTGPELEICGYSCEDHFHES 60 M R VT+A C LNQWA+DFEGN RI+ SI+EAK A R GPELE+ GY CEDHF ES Sbjct: 1 MERYVTIASCQLNQWAMDFEGNRLRIIDSIKEAKRQNASLRVGPELEVTGYGCEDHFLES 60 Query: 61 DTYLHSWQVLVELLKSPTCKDILIDVGMPVQHRNVSYNCRVAFFNRKIILIRPKMILCDD 120 DTY HSW++L ++ P C+DIL+D+GMPV H+ + +NCR+ N KI+LIRPK+ LCDD Sbjct: 61 DTYYHSWEMLCSIIHDPDCQDILLDIGMPVMHKAMRHNCRILALNGKILLIRPKIWLCDD 120 Query: 121 GNYRETRWFSCWTKDRQVEDFYLPRMITAVTNQSTVPIGDAVISTRDTCIGFEICEELWN 180 GN+RE+RWF+ W + R VE YLP + NQ+TVPIGDA++ +T +G E CEEL+ Sbjct: 121 GNFRESRWFTPWLRPRVVETHYLPTFVAKSLNQTTVPIGDAILQCNETVVGVETCEELFT 180 Query: 181 PQSRHIPLSLDGVEIISNGSGSYMELRKAYVTVDLVKSATFKSGGAYLFSNLRGCDGQRI 240 P S HI ++LDGVEI N SGS+ ELRK V+L+++AT K GG YL+SN RGCDG R+ Sbjct: 181 PNSPHIDMALDGVEIFINASGSHHELRKLTTRVNLIQNATEKCGGIYLYSNQRGCDGGRL 240 Query: 241 YFNGCSCVAVNGEIVSRGQQFGLIDVEVTTATIDLEDIRSYRAKNRSRCHLAASNKPFPR 300 Y++G S + NG+++++G QF L DVEV +AT+D++ +RSYR + S + + R Sbjct: 241 YYDGSSMIFANGKMLAQGHQFSLKDVEVISATVDVDTVRSYRFQ-PSHGIQGVTRPSYER 299 Query: 301 IFVDVSLS---DDEDIHLTTNPPIQWHYLSPEEEISLGPACWLWDYLRRSGQGGFFLPLS 357 I V+ SLS D DI+ PI+ PEEEI+ GPACWLWDYLRRS GFFLPLS Sbjct: 300 IHVNFSLSSYQQDYDIYRKPTDPIEVTIPLPEEEITFGPACWLWDYLRRSHAAGFFLPLS 359 Query: 358 GGVDSSSTACIVFSMCTQICEAIKKGESQVLYDVRKIMCQPDYTPSDPMELCNKLLVTCY 417 GG+DS STA +V+SMC +C+A+++ ++QVL DVR+I+ P Y+ +DP +L N L T + Sbjct: 360 GGLDSCSTAVLVYSMCRIVCKAMEEDDAQVLSDVRRIVGDPSYSSTDPKKLLNHLFYTAF 419 Query: 418 MASENSSRETKQRASQLASQIGSYHFPILIDTAVNAALGIFTAATGLLPIFKSKGGCPRQ 477 M SE+SS+ET+ RA +L+S IGSYH + IDT +A + +F TG P F+S GG + Sbjct: 420 MGSEHSSKETRSRAKELSSLIGSYHTDVNIDTMTSAVVKLFALVTGKTPQFRSNGGTNAE 479 Query: 478 NLALQNIQARIRMVLSYLFAQLMXXXXXXXXXXXXXXSSNVDEALRGYMTKYDCSSADIN 537 NLALQNIQAR RM+L YLFAQL+ SSNVDE LRGY+TKYDCSSADIN Sbjct: 480 NLALQNIQARSRMLLGYLFAQLLPWVRGYSGSLLVLGSSNVDECLRGYLTKYDCSSADIN 539 Query: 538 PIGGISKTDLKSFLHYAKNRFFLPSLSEILEAPPTAELEPLADGQITQTDEQDMGMTYAE 597 PIGGISKTDLKSFL YAK LP L E L+A PTAELEP + + Q+DE DMGMTYAE Sbjct: 540 PIGGISKTDLKSFLRYAKEALDLPILQEFLDATPTAELEPTTESYV-QSDEADMGMTYAE 598 Query: 598 LSEFGTLRKTYKCGPYSMFEKLVHKWSDKCTPKEVAEKVKHFFRCYAINRHKMTVLTPSY 657 LS FG LRK KCGPYSMF +L+H+W D+ +P +VAEKVK FF Y INRHKMT LTPSY Sbjct: 599 LSVFGRLRKISKCGPYSMFTQLMHQWGDRLSPSQVAEKVKRFFHYYGINRHKMTTLTPSY 658 Query: 658 HAETYSPDDNRFDLRPFLYRVHWNWQFKTID 688 HAETY DDNR+DLR FLY W WQ K ID Sbjct: 659 HAETYGVDDNRYDLRQFLY-PSWTWQNKKID 688 >SPAC30C2.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 457 Score = 30.3 bits (65), Expect = 0.94 Identities = 15/49 (30%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Query: 384 ESQVLYDVRKIMCQPDYTPSDPMELCNKLLVTCYMASENSSRETKQRAS 432 E + + D + C D+ P ELCN +L C+ E++ +TK+ S Sbjct: 216 EIEEMKDSNGMFCDADHVPLQGQELCNGILEECFSFLEDA--KTKEGLS 262 >SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex subunit Orp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 30.3 bits (65), Expect = 0.94 Identities = 22/100 (22%), Positives = 45/100 (45%), Gaps = 5/100 (5%) Query: 351 GFFLPLSGGVDSSSTACIVFSMCTQICEAIKKGESQVLYD----VRKIMCQPDYTPSDPM 406 G F+PL GG + + ++CT++ +++ + YD V + +C+ P D Sbjct: 32 GLFVPLIGGKEPIEYVKLRENLCTKVLNELEEITTHTTYDSNARVLRQLCEHASEPVDET 91 Query: 407 ELCNKLLVTCYMASENSSRETKQRASQLASQIGSYHFPIL 446 L +V C +S + + ++ +IG +F +L Sbjct: 92 GLLRTAIV-CDKSSNSMHLKFYEQLKDDMEKIGWKNFVVL 130 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 27.9 bits (59), Expect = 5.0 Identities = 13/43 (30%), Positives = 21/43 (48%) Query: 418 MASENSSRETKQRASQLASQIGSYHFPILIDTAVNAALGIFTA 460 + SEN S + ++ S+ S FP+LI T + + F A Sbjct: 2033 LESENQSEQALSALREIISRRSSTIFPVLIPTLIKKPVSAFNA 2075 >SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2176 Score = 27.9 bits (59), Expect = 5.0 Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 3/45 (6%) Query: 273 IDLEDIRSYRAKNRSRCHLAASNK---PFPRIFVDVSLSDDEDIH 314 IDL+D + + HLA + +P I +D + D ED+H Sbjct: 2022 IDLDDEKRTELLHMDNAHLAKCAEFINKYPDIDIDFEIEDSEDVH 2066 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.322 0.136 0.425 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,211,818 Number of Sequences: 5004 Number of extensions: 137092 Number of successful extensions: 269 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 263 Number of HSP's gapped (non-prelim): 6 length of query: 697 length of database: 2,362,478 effective HSP length: 78 effective length of query: 619 effective length of database: 1,972,166 effective search space: 1220770754 effective search space used: 1220770754 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 57 (27.1 bits)
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