BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA001159-TA|BGIBMGA001159-PA|IPR007261|Vacuolar protein sorting 36 (401 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_35538| Best HMM Match : Vps36 (HMM E-Value=3.1e-07) 69 7e-12 SB_14816| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.31 SB_42304| Best HMM Match : NHL (HMM E-Value=0) 33 0.41 SB_2095| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.41 SB_35254| Best HMM Match : Vicilin_N (HMM E-Value=0.066) 32 0.95 SB_12377| Best HMM Match : SpoU_methylase (HMM E-Value=4.3) 30 2.9 SB_36982| Best HMM Match : adh_short (HMM E-Value=1.4e-05) 30 3.8 SB_11152| Best HMM Match : Myosin_head (HMM E-Value=0) 30 3.8 SB_47732| Best HMM Match : Pkinase (HMM E-Value=0.0016) 29 5.0 SB_40301| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.7 SB_34906| Best HMM Match : Cadherin (HMM E-Value=0) 29 6.7 SB_33744| Best HMM Match : SMC_N (HMM E-Value=0) 29 8.8 SB_55616| Best HMM Match : Exo_endo_phos (HMM E-Value=0.032) 29 8.8 SB_50413| Best HMM Match : GRP (HMM E-Value=0.15) 29 8.8 SB_17360| Best HMM Match : 7tm_1 (HMM E-Value=1.8e-26) 29 8.8 >SB_35538| Best HMM Match : Vps36 (HMM E-Value=3.1e-07) Length = 102 Score = 68.9 bits (161), Expect = 7e-12 Identities = 30/52 (57%), Positives = 39/52 (75%) Query: 346 IQENGYLTPEKLSQIANVSVLLARERLFTTERLGLACRDESIEGLAFYPNRF 397 + + G LT E+L+ A VSV+LA+ERL TE+ G ACRD+S+EGL FYPN F Sbjct: 46 LDDKGSLTAEELAHFAEVSVMLAKERLLVTEKAGKACRDDSVEGLRFYPNLF 97 Score = 39.1 bits (87), Expect = 0.006 Identities = 20/45 (44%), Positives = 29/45 (64%), Gaps = 1/45 (2%) Query: 276 GGIMSLADVWCRVNRARGLELVSPEDLLNACKLLRTVDAPMSLRK 320 GG+M+L+DV+CR NRARG+EL + L A +L + + L K Sbjct: 26 GGMMALSDVYCRFNRARGMEL-DDKGSLTAEELAHFAEVSVMLAK 69 >SB_14816| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 3760 Score = 33.5 bits (73), Expect = 0.31 Identities = 43/190 (22%), Positives = 89/190 (46%), Gaps = 13/190 (6%) Query: 179 EEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGDISEDDTVRFKSYLMS 238 +E+ R + + A +++T LM + + SK+ +I++ + ED + L + Sbjct: 2897 KEELRQKETELHEAREEITVLMNR----IESSKD--KQIKKVMKAVCEDIEAVEEKELHA 2950 Query: 239 LGIDDPVTRDAFRSDSEYYLGLAQQVSDMMVAVLLEC--GGIMSLADVWCRVNRARGLEL 296 +D + + +D E L ++ +M + LE G +M+L D + ++ R ++ Sbjct: 2951 AVVDQLDSAPSNSADREEVTRL--KIENMRMKARLEAADGKLMNLQDELHKTSKDRVIDE 3008 Query: 297 VSPEDLLNACKLLRTVDAPMSLRKFPSGACVLQLNSNRD-EEIAKSTSDMIQENGYLTPE 355 + +D + K L+ + L++ A +QLN +D E++ K ++ +EN L Sbjct: 3009 KTIKDKESENKTLK--EEEQDLKRRLRQADNVQLNLRKDLEDLQKEKEELRKENENLKKR 3066 Query: 356 KLSQIANVSV 365 KLS + V V Sbjct: 3067 KLSIVEKVKV 3076 >SB_42304| Best HMM Match : NHL (HMM E-Value=0) Length = 1279 Score = 33.1 bits (72), Expect = 0.41 Identities = 30/96 (31%), Positives = 46/96 (47%), Gaps = 6/96 (6%) Query: 12 FDGENYLKR-DKNVKIYDGDDKTQFIDGE----VLLTTHRLLWGKPGDIPKGLVCLSLHL 66 F G+ +++R ++ D D+K +F D + LLTT R+ K G K V LSL L Sbjct: 1114 FKGQEFVRRLTTSLDQVDSDEKLRFADPDDKRNTLLTTKRVYVMKVGSACKENVVLSLKL 1173 Query: 67 YYVFCLE-EESGGVFGLGGPKRIIMHLGPALPGKRP 101 + ++ + +E G + L KRI LP P Sbjct: 1174 HELYKTQHKEIEGTYYLELVKRIAGMQHHNLPSPHP 1209 >SB_2095| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 182 Score = 33.1 bits (72), Expect = 0.41 Identities = 22/86 (25%), Positives = 46/86 (53%), Gaps = 6/86 (6%) Query: 205 EMVSLSKNISTKIREKQG--DISED---DTVRFKSYLMSLGIDDPVTR-DAFRSDSEYYL 258 E +KNI+ K ++++G D S + +RFK+ L L ++ + + +SD++++ Sbjct: 57 EQYQYTKNITEKFQKQEGPADTSHNIIYSAMRFKNALDDLTLEPDIFHLNPKKSDTDWFK 116 Query: 259 GLAQQVSDMMVAVLLECGGIMSLADV 284 + + + V VLL C I+++ V Sbjct: 117 NIIRSLDICQVKVLLPCTEIVNICPV 142 >SB_35254| Best HMM Match : Vicilin_N (HMM E-Value=0.066) Length = 909 Score = 31.9 bits (69), Expect = 0.95 Identities = 33/143 (23%), Positives = 60/143 (41%), Gaps = 7/143 (4%) Query: 174 IERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREK----QGDISEDDT 229 +ER E+ + A D+ + L E+ +L K++STK ++ + +I + D Sbjct: 570 LERKREDYNTAADERRYQMAKKKDTLQATRSELETLVKDLSTKTGKERLALEKEIQKKDK 629 Query: 230 V--RFKSYLMSLGIDDPVTRDAFRSDSEYYL-GLAQQVSDMMVAVLLECGGIMSLADVWC 286 + K+YL L +D R A + + + L +Q+ + LE + L D Sbjct: 630 IYAEQKAYLHQLETEDAKIRQAVDQELDNEMSALTKQLEQNQAFLKLERLNLEELQDAKA 689 Query: 287 RVNRARGLELVSPEDLLNACKLL 309 R L L ++ N K+L Sbjct: 690 AAIRKDELHLKEAQEKYNEVKIL 712 >SB_12377| Best HMM Match : SpoU_methylase (HMM E-Value=4.3) Length = 165 Score = 30.3 bits (65), Expect = 2.9 Identities = 21/73 (28%), Positives = 34/73 (46%), Gaps = 2/73 (2%) Query: 310 RTVDAPMSLRKFPSGACVLQLNSN-RDEEIAKSTSDMIQENGYLTPEKLSQIANVSVLLA 368 +T D P L++ GAC +Q +SN ++ T I N + +S + + LL+ Sbjct: 78 QTPDLPKFLQEMKEGACEIQFSSNAHSYYLSTHTKPKIVSNRLIWTPSMSLLQQATPLLS 137 Query: 369 RERLFTTERLGLA 381 R F RL L+ Sbjct: 138 VPRCF-ARRLTLS 149 >SB_36982| Best HMM Match : adh_short (HMM E-Value=1.4e-05) Length = 258 Score = 29.9 bits (64), Expect = 3.8 Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 7/83 (8%) Query: 268 MVAVLLECGGIMSLADVWCRVNRARGLELVSPEDLLNACKLLRTVDAPMSLRK-FPSGAC 326 M + L G + +CR ARG V L C+ LR +P Sbjct: 1 MAPLALVQGASRGIGLQFCRHLLARGNGFV-----LATCRNPTNAKELQDLRSNYPKNMA 55 Query: 327 VLQLNSNRDEEIAKSTSDMIQEN 349 + +L+++ +E+I K+T+DMI+EN Sbjct: 56 IFELDAHNEEKI-KATADMIKEN 77 >SB_11152| Best HMM Match : Myosin_head (HMM E-Value=0) Length = 1997 Score = 29.9 bits (64), Expect = 3.8 Identities = 13/64 (20%), Positives = 33/64 (51%) Query: 164 NSKIRSGIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGD 223 N K S I+ + + +EEQ A +Q+++ + M++ +E + K K+ +++ Sbjct: 1200 NKKRESDIIKLRKDLEEQALAHEQAVNSMRSKQNQQMQEMQEELDQVKKTKAKLEKEKAQ 1259 Query: 224 ISED 227 ++ + Sbjct: 1260 LTNE 1263 >SB_47732| Best HMM Match : Pkinase (HMM E-Value=0.0016) Length = 318 Score = 29.5 bits (63), Expect = 5.0 Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Query: 215 TKIREKQGDISEDDTVRFKSYLMSLGIDDPVTRDAFRSD 253 T I + GD E++ RF+SY+ L DDP D F+ D Sbjct: 197 TYISDFLGDAIEENPKRFRSYIKQLKNDDPGVAD-FKVD 234 >SB_40301| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2653 Score = 29.1 bits (62), Expect = 6.7 Identities = 18/68 (26%), Positives = 34/68 (50%), Gaps = 1/68 (1%) Query: 168 RSGIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGDISED 227 ++ + E I E A D SI+ + LTK + + + + ++T + ++QG +E+ Sbjct: 1356 QASLAEAESKIAELQAAYDHSIAEK-ETLTKNIAQTAARLKRASKLTTALGDEQGRWTEN 1414 Query: 228 DTVRFKSY 235 VR SY Sbjct: 1415 VAVRMHSY 1422 >SB_34906| Best HMM Match : Cadherin (HMM E-Value=0) Length = 3922 Score = 29.1 bits (62), Expect = 6.7 Identities = 14/29 (48%), Positives = 18/29 (62%), Gaps = 1/29 (3%) Query: 81 GLGGPKRIIMHLGPALPGKRPGPAVVSPF 109 G GP +++ P PG+RPG VVSPF Sbjct: 3599 GWTGPTCVLIVTAPN-PGQRPGSRVVSPF 3626 >SB_33744| Best HMM Match : SMC_N (HMM E-Value=0) Length = 1014 Score = 28.7 bits (61), Expect = 8.8 Identities = 13/38 (34%), Positives = 24/38 (63%) Query: 177 SIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNIS 214 SIEEQ D++I+ + + K EKAK+ ++++ +S Sbjct: 656 SIEEQVTKVDENIAKCEEQVEKCSEKAKQTQAINEKLS 693 >SB_55616| Best HMM Match : Exo_endo_phos (HMM E-Value=0.032) Length = 296 Score = 28.7 bits (61), Expect = 8.8 Identities = 28/124 (22%), Positives = 56/124 (45%), Gaps = 4/124 (3%) Query: 244 PVTRDAFRSD-SEYYLGLAQQVSDMMVAVLLECGGIMSLADVWCRVNRARGLELVSPEDL 302 P FR D S+ Y G+ V D+ L E + +NR + + + + + Sbjct: 131 PSNYSVFRKDRSDGYGGVLLAVKDLSCHELYELNSDCETVWIKVSINRRKHVYIGAFYNP 190 Query: 303 LNACKLLRTVDAPMSLRKFPSGACVLQLNS--NRDEEIAKSTSDMIQENGYLTP-EKLSQ 359 ++C+ L +D +S + SG V+ L N +I T+ + + + ++T EKL + Sbjct: 191 DSSCEALDELDRSLSKLQRKSGNSVIYLGGDFNLGGDICWETNTIARGSRHVTACEKLLE 250 Query: 360 IANV 363 + ++ Sbjct: 251 MCSI 254 >SB_50413| Best HMM Match : GRP (HMM E-Value=0.15) Length = 487 Score = 28.7 bits (61), Expect = 8.8 Identities = 15/39 (38%), Positives = 23/39 (58%) Query: 340 KSTSDMIQENGYLTPEKLSQIANVSVLLARERLFTTERL 378 K+TS ++ Y T EKL++++NV L ER T R+ Sbjct: 89 KTTSQEYEDRLYKTQEKLAELSNVRQQLENERENLTIRM 127 >SB_17360| Best HMM Match : 7tm_1 (HMM E-Value=1.8e-26) Length = 715 Score = 28.7 bits (61), Expect = 8.8 Identities = 37/157 (23%), Positives = 59/157 (37%), Gaps = 7/157 (4%) Query: 116 FKDGVDTAFYKALSDAVAAKAWERQTPIDXXXXXXXXXXXXXXXXXXXNSKIRSGIVGIE 175 +K GV T S VAA +R TP+ + G+ G+ Sbjct: 506 WKPGVATP---TTSGVVAAPRKKRVTPVVKKRKVVPMGELTDTQPLIVSEDEEEGVAGVS 562 Query: 176 -RSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGDISEDDTVRFKS 234 S EE+ ++SI A K ++K + +T+ E+ + D+ F Sbjct: 563 TNSTEEEEEEEEKSILAAVAPPGKKLKKVTA-TEVDSGDATRGSEESDYETVDENSCFDE 621 Query: 235 YLMSLGIDDPVTRDAFRSDSEYYLGLAQQVSDMMVAV 271 S G DD V A+ D+E LA+ + VA+ Sbjct: 622 E--SSGGDDDVFEQAYMEDAEREQHLAEDDTPSSVAL 656 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,404,508 Number of Sequences: 59808 Number of extensions: 499161 Number of successful extensions: 1254 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 1245 Number of HSP's gapped (non-prelim): 20 length of query: 401 length of database: 16,821,457 effective HSP length: 84 effective length of query: 317 effective length of database: 11,797,585 effective search space: 3739834445 effective search space used: 3739834445 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 61 (28.7 bits)
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