Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001159-TA|BGIBMGA001159-PA|IPR007261|Vacuolar protein
sorting 36
(401 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04920.1 68418.m00519 vacuolar protein sorting 36 family prot... 161 6e-40
At2g21730.1 68415.m02585 mannitol dehydrogenase, putative simila... 31 1.0
At5g61950.1 68418.m07776 ubiquitin carboxyl-terminal hydrolase-r... 30 2.4
At4g28715.1 68417.m04107 myosin heavy chain, putative similar to... 30 3.1
At2g33150.1 68415.m04062 acetyl-CoA C-acyltransferase, putative ... 30 3.1
At2g30700.1 68415.m03745 expressed protein 29 4.1
At1g16630.1 68414.m01992 expressed protein 29 5.5
At3g54170.1 68416.m05988 FKBP12 interacting protein (FIP37) iden... 29 7.2
At3g13227.1 68416.m01659 serine-rich protein-related contains so... 29 7.2
At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putativ... 28 9.6
At4g18830.1 68417.m02779 ovate family protein 52% similar to ova... 28 9.6
At3g50460.1 68416.m05519 hypersensitive response protein 2 (HR2)... 28 9.6
At1g32870.1 68414.m04050 no apical meristem (NAM) family protein... 28 9.6
>At5g04920.1 68418.m00519 vacuolar protein sorting 36 family protein
/ VPS36 family protein contains Pfam PF04132: Vacuolar
protein sorting 36
Length = 440
Score = 161 bits (392), Expect = 6e-40
Identities = 122/389 (31%), Positives = 188/389 (48%), Gaps = 30/389 (7%)
Query: 23 NVKIYDGDDKTQFI---DGEVLLTTHRLLWGKPGDIPKGLVCLSLHLYYVFCLEEESGGV 79
++ I DD +F G ++LTTHRL+W P + V S+ L V + +
Sbjct: 39 SIDIDSEDDPPRFTALRSGNLILTTHRLIW-IPSQSNES-VPSSIPLSAVTHIYSHKKSI 96
Query: 80 FGLGGPKRIIMHLGPALPGKRPGPAVVSPFHYVKLSFKDGVDTAFYKALSDAVAAKAWER 139
+ RI P G VV+ K F DG F L + +AWE
Sbjct: 97 KSMFHSPRIRFQADP-------GSIVVTIVFRGKGDF-DG----FLSKLWECWRGRAWEE 144
Query: 140 QTPIDXXXXXXXXXXXXXXXXXXXNSKIRSGIVGIERSIEEQHRATDQSISVAFQDLTKL 199
+ + + G+ GI R +EQ +TD+S+ AFQDL L
Sbjct: 145 EEKSESETSKSGSGTVAQGLYGNDGTVRMVGLAGILRKEQEQWESTDKSLQDAFQDLNAL 204
Query: 200 MEKAKEMVSLSKNISTKIRE---KQGDISEDDTVRFKS----YLMSLGIDDPVTRDAFRS 252
M KAKEMVSL++ + K+ Q ++D+ + K +++S+GI PVT+++ +
Sbjct: 205 MSKAKEMVSLAEKMRQKLLSAPSSQNGSTDDEEMGSKEEMQQWMLSVGIISPVTKES--A 262
Query: 253 DSEYYLGLAQQVSDMMVAVLLECGGIMSLADVWCRVNRARGLELVSPEDLLNACKLLRTV 312
+ Y+ L++Q++D + L + GG++SL D++ NRARG EL+SP+DL AC L
Sbjct: 263 GALYHQELSRQLADFVRIPLEKAGGMISLTDMYYHFNRARGTELISPDDLWQACTLWEKF 322
Query: 313 DAPMSLRKFPSGACVLQLNSNRDEEIAKSTSDMIQENGYL----TPEKLSQIANVSVLLA 368
D P+ LRKF SG V+Q S+ DEE+ ++ + L T + ++ +A
Sbjct: 323 DVPVMLRKFDSGVMVIQNKSHSDEEVMSRIRMLVTKTETLRVGVTASDAALTLKIAPAMA 382
Query: 369 RERLFTTERLGLACRDESIEGLAFYPNRF 397
+E L + E GL CRD S +GL FY N F
Sbjct: 383 KEHLLSAETKGLLCRDMSPDGLRFYFNLF 411
>At2g21730.1 68415.m02585 mannitol dehydrogenase, putative similar
to ELI3-2 (SP|Q02972), sinapyl alcohol dehydrogenase
(Populus tremuloides) (gi:14279694); contains Pfam
zinc-binding dehydrogenase domain PF00107
Length = 376
Score = 31.5 bits (68), Expect = 1.0
Identities = 29/96 (30%), Positives = 44/96 (45%), Gaps = 13/96 (13%)
Query: 13 DGENYLKR---DKNVKIYDGDDKTQFIDGEVLLTTHRLLW----GKPGDIPKGLVCLSLH 65
D ENY + N + DG + Q +V++ HR + G P D L+C +
Sbjct: 106 DLENYCPKVVFTYNSRSSDGTSRNQGGYSDVIVVDHRFVLSIPDGLPSDSGAPLLCAGIT 165
Query: 66 LY---YVFCLEEESG---GVFGLGGPKRIIMHLGPA 95
+Y + + +ESG GV GLGG I + +G A
Sbjct: 166 VYSPMKYYGMTKESGKRLGVNGLGGLGHIAVKIGKA 201
>At5g61950.1 68418.m07776 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of
unknown function (DUF627)
Length = 1132
Score = 30.3 bits (65), Expect = 2.4
Identities = 22/62 (35%), Positives = 34/62 (54%), Gaps = 9/62 (14%)
Query: 194 QDLTKLMEKAKEMVSLSKNI-----STKIR--EKQGDISEDDTVRFKSYLMSLGIDDPVT 246
+DL +E A+ M++ SK K+R EK + S+DD RF+SY +G+D +
Sbjct: 145 KDLEGFIEDAESMIAASKTSPLQKPEPKVRDPEKTPEPSKDDFQRFRSYW--VGLDVKIK 202
Query: 247 RD 248
RD
Sbjct: 203 RD 204
>At4g28715.1 68417.m04107 myosin heavy chain, putative similar to
myosin [Arabidopsis thaliana] gi|499047|emb|CAA84066
Length = 639
Score = 29.9 bits (64), Expect = 3.1
Identities = 19/78 (24%), Positives = 43/78 (55%), Gaps = 2/78 (2%)
Query: 164 NSKIRSGIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGD 223
N K++S + +++ I+E + ++ + + L + +E +V+L K +++EK D
Sbjct: 108 NEKLKSLVSSLDQKIDETEKKFEERSKINEERLKQAIEAETTIVNL-KTAVHELQEKILD 166
Query: 224 I-SEDDTVRFKSYLMSLG 240
+ SE+ +R KS + + G
Sbjct: 167 VESENKILRQKSLIQASG 184
>At2g33150.1 68415.m04062 acetyl-CoA C-acyltransferase, putative /
3-ketoacyl-CoA thiolase, putative similar to
3-ketoacyl-CoA thiolase (E.C. 2.3.1.16) from
[Arabidopsis thaliana] GI:2981616, [Cucumis sativus]
GI:393707, [Cucurbita cv. Kurokawa Amakuri] GI:1694621;
contains InterPro accession IPR002155: Thiolase
Length = 462
Score = 29.9 bits (64), Expect = 3.1
Identities = 23/55 (41%), Positives = 28/55 (50%), Gaps = 4/55 (7%)
Query: 219 EKQGDISEDDTVRFKSYLMSLGIDDPVTRDAFRSDSEYYLGLAQQVSDMMVAVLL 273
EK +S DD +R + L SLG PV F+ D G + QVSD AVLL
Sbjct: 253 EKPITVSVDDGIRPTTTLASLGKLKPV----FKKDGTTTAGNSSQVSDGAGAVLL 303
>At2g30700.1 68415.m03745 expressed protein
Length = 480
Score = 29.5 bits (63), Expect = 4.1
Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 9/74 (12%)
Query: 286 CRVNRARGLELVSPEDLLNACKLLRTVDAP-----MSLRKFPSGACVLQLNSNRDEEI-A 339
C+VN+A LE P +++ AC R V AP SL + SG L +N+ + A
Sbjct: 299 CKVNKACPLEFKEPTEVIKAC---RNVAAPSPSCCSSLNAYISGIQNQMLITNKQAIVCA 355
Query: 340 KSTSDMIQENGYLT 353
M+++ G +T
Sbjct: 356 TVIGSMLRKGGVMT 369
>At1g16630.1 68414.m01992 expressed protein
Length = 845
Score = 29.1 bits (62), Expect = 5.5
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Query: 166 KIRSGIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQG-DI 224
KI S + RS ++ H SV+F D+ ++ + K++ + + + ++RE++ DI
Sbjct: 115 KILSDKNEVSRSFDKSHHQVQVKSSVSFSDVISIIGEDKDVDQICIDETKQLREEESHDI 174
Query: 225 SEDD 228
+ D
Sbjct: 175 TVSD 178
>At3g54170.1 68416.m05988 FKBP12 interacting protein (FIP37)
identical to FKBP12 interacting protein (FIP37)
GI:3859944 from [Arabidopsis thaliana]
Length = 330
Score = 28.7 bits (61), Expect = 7.2
Identities = 18/68 (26%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Query: 164 NSKIRSGIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKN---ISTKIREK 220
N+++RS G+ + +EE ++S KL EK KE+ + K +S + +K
Sbjct: 252 NAELRSQFEGLYKHMEELTNDVERSNETVIILQEKLEEKEKEIERVKKGLEIVSELVGDK 311
Query: 221 QGDISEDD 228
+ ++ E D
Sbjct: 312 KDEVDEID 319
>At3g13227.1 68416.m01659 serine-rich protein-related contains some
similarity to serine-rich proteins
Length = 111
Score = 28.7 bits (61), Expect = 7.2
Identities = 13/39 (33%), Positives = 22/39 (56%)
Query: 200 MEKAKEMVSLSKNISTKIREKQGDISEDDTVRFKSYLMS 238
ME +E + K++E + D+S+DD R ++Y MS
Sbjct: 1 MEGKEEEAKRRMMMKIKLKETEADVSQDDIKRQEAYNMS 39
>At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putative
strong similarity to GDP dissociation inhibitor protein
OsGDI1 [Oryza sativa] GI:2384758; contains Pfam profile
PF00996: GDP dissociation inhibitor
Length = 365
Score = 28.3 bits (60), Expect = 9.6
Identities = 23/64 (35%), Positives = 33/64 (51%), Gaps = 4/64 (6%)
Query: 180 EQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGDISEDDTVRFKSYLMSL 239
E+ RA I V QD + K+ E + LSK + +I K G EDDT+ F + ++L
Sbjct: 55 EKRRARKFFIYV--QDYDEKDPKSHEGLDLSKVTAREIISKYG--LEDDTIDFIGHALAL 110
Query: 240 GIDD 243
DD
Sbjct: 111 HNDD 114
>At4g18830.1 68417.m02779 ovate family protein 52% similar to ovate
protein (GI:23429649) [Lycopersicon esculentum];
contains TIGRFAM TIGR01568 : uncharacterized
plant-specific domain TIGR01568
Length = 349
Score = 28.3 bits (60), Expect = 9.6
Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 4/53 (7%)
Query: 308 LLRTVDAPMSLRKFPSGACVLQLNSNRDEEIAKSTSDMIQENGYLTPEKLSQI 360
L+ T D M F V++ +S+ ++ S +MI ENG PE+L ++
Sbjct: 271 LIETADGGMENESF----AVVKCSSDPQKDFRDSMIEMIMENGINHPEELKEL 319
>At3g50460.1 68416.m05519 hypersensitive response protein 2 (HR2)
identical to HR2 [Arabidopsis thaliana] GI:12958165;
contains Pfam profile PF05659: Arabidopsis
broad-spectrum mildew resistance protein RPW8
Length = 205
Score = 28.3 bits (60), Expect = 9.6
Identities = 12/37 (32%), Positives = 23/37 (62%)
Query: 171 IVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMV 207
I + +E+ ++ +D+S+ F+DL L+EKA +V
Sbjct: 47 ITPLMAKVEKLNKESDESLRKVFEDLKHLLEKAVVLV 83
>At1g32870.1 68414.m04050 no apical meristem (NAM) family protein
similar to to NAC2 (GI:645671) [Arabidopsis thaliana];
contains Pfam PF02365: No apical meristem (NAM) protein
Length = 528
Score = 28.3 bits (60), Expect = 9.6
Identities = 14/52 (26%), Positives = 26/52 (50%)
Query: 193 FQDLTKLMEKAKEMVSLSKNISTKIREKQGDISEDDTVRFKSYLMSLGIDDP 244
+ ++ LME E S+ + + G +++DD V SY + LG+ +P
Sbjct: 260 YLEINDLMEPEPEPTSVEVMENNWNEDGSGLLNDDDFVGADSYFLDLGVTNP 311
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.319 0.137 0.397
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,974,552
Number of Sequences: 28952
Number of extensions: 371633
Number of successful extensions: 934
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 926
Number of HSP's gapped (non-prelim): 15
length of query: 401
length of database: 12,070,560
effective HSP length: 83
effective length of query: 318
effective length of database: 9,667,544
effective search space: 3074278992
effective search space used: 3074278992
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 60 (28.3 bits)
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