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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001159-TA|BGIBMGA001159-PA|IPR007261|Vacuolar protein
sorting 36
         (401 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharom...    68   2e-12
SPBC651.05c |dot2||EAP30 family protein Dot2|Schizosaccharomyces...    38   0.003
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos...    29   1.5  
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu...    28   2.7  
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos...    27   3.5  
SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein Bms1|Schizosacc...    27   4.7  
SPAC2C4.09 |||DUF1640 family protein|Schizosaccharomyces pombe|c...    27   4.7  
SPBC21.03c |||DUF55 family protein|Schizosaccharomyces pombe|chr...    26   8.2  

>SPBC3B9.09 |vps36||RBZ zinc finger protein
           Vps36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 467

 Score = 68.1 bits (159), Expect = 2e-12
 Identities = 57/235 (24%), Positives = 107/235 (45%), Gaps = 9/235 (3%)

Query: 170 GIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAKEMVSLSKNISTKIREKQGDISEDDT 229
           GI  +E+S E Q     +++  AFQDL      AK+ +SL+   + K+    G    D  
Sbjct: 221 GIHDLEQSHEMQLAKNGRTLVHAFQDLDAFFSLAKDTMSLADQFAEKMDGLTGTQQSDKV 280

Query: 230 VRFKSYLMSLGI--DDPVTRDAFRSDSEYY-LGLAQQVSDMMVAVLLECGGIMSLADVWC 286
            +  +    LG+   + +      ++S  Y + L + +S+++   L      +++   W 
Sbjct: 281 QQLLNKSNQLGVLRGNHLDNVPVSANSRLYDIELCKSISEVLRTRLKADTDTVTMTQAWA 340

Query: 287 RVNRARGLELVSPEDLLNACKLLRTVDAPMSLRKFPSGACVLQL-NSNRDEEIAKSTSDM 345
             NR+R   L+ P   + AC+L+ +++  ++  K  SG  + QL  SN   ++  +  ++
Sbjct: 341 IYNRSRKANLIPPTRFVKACELIDSMEVGITTSKMNSGLILFQLKTSNHSGKLLSAILEV 400

Query: 346 IQENGYLTPEKLSQIANVSVLLARERLFTTERLGLACRD---ESIEGLAFYPNRF 397
           +  N   T  K +   + S+ +  ERL+  E  G   RD   ES   L ++ N F
Sbjct: 401 M--NPSTTALKCAMRLHWSIGVTLERLYQAEMDGYIVRDVYEESGSSLLYWKNEF 453


>SPBC651.05c |dot2||EAP30 family protein Dot2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 252

 Score = 37.5 bits (83), Expect = 0.003
 Identities = 40/166 (24%), Positives = 74/166 (44%), Gaps = 17/166 (10%)

Query: 201 EKAKEMVSLSKNISTKIREKQGDISEDDTVR--FKSYLMSLGIDDPVTRD-----AFRSD 253
           E A ++ +  + + T  RE   +I ++   R  F    + +G+D  V+       A    
Sbjct: 33  EIASQLSTFQEALKTFAREHATEIKQNSQFRNTFVKLALKIGLDPFVSGSDESAWAAVGM 92

Query: 254 SEYYLGLAQQVSDMMVAVLLECGGIMSLADVWCRV----NRARGLELVSPEDLLNACKLL 309
           +E+Y  +A +V ++  A  +E GG++S++ V CR     N A G E +   D++ A   L
Sbjct: 93  NEFYYQVAVRVIEVCYATQMENGGLLSVSQV-CRFLNEENEAFGHEWLRETDVVRAVDSL 151

Query: 310 RTVDAPMSLRKFPSGACV--LQLNSNRDEEIAKSTSDMIQENGYLT 353
             +     L K      +  L L  N D+ +     +++   GY+T
Sbjct: 152 APLGPGFVLEKIAGKQYIRSLPLELNTDQNVVLEAVEIL---GYVT 194


>SPAC694.06c |mrc1||mediator of replication checkpoint 1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1019

 Score = 28.7 bits (61), Expect = 1.5
 Identities = 22/84 (26%), Positives = 34/84 (40%), Gaps = 6/84 (7%)

Query: 195 DLTKLMEKAKEMVSLSKNISTKIREKQGDISEDDTVRFKSYLMSLGIDDPVTRDAFRSDS 254
           D   L E       + K +S    EK GD+S D+++  KSY        P+     R   
Sbjct: 917 DNNSLEEVLPIFPGVRKLVSNSQSEKIGDLSNDNSMGAKSY------KTPIISSTQRPQG 970

Query: 255 EYYLGLAQQVSDMMVAVLLECGGI 278
             + GL  Q S   ++  ++ G I
Sbjct: 971 RKFRGLMNQSSKADISRTVDAGSI 994


>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1405

 Score = 27.9 bits (59), Expect = 2.7
 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 1/54 (1%)

Query: 347 QENGYLTPEKLSQIANVSVLLARERLFTTERLGLACRDESIEGLAFYPNRFLLE 400
           ++NGY+ P+K   I   +     E     E LG   +D+   GL F P + L E
Sbjct: 787 KKNGYIVPDKQQSIRRHAEAFGAEDGLQEESLGKK-KDKYKGGLVFEPQKGLYE 839


>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 640

 Score = 27.5 bits (58), Expect = 3.5
 Identities = 16/58 (27%), Positives = 36/58 (62%), Gaps = 2/58 (3%)

Query: 168 RSGIVGIERSIEEQHRATDQSISVAFQDLTKLMEKAK-EMVSLS-KNISTKIREKQGD 223
           +SGI+   R ++EQ+  TD ++++  +D+T  + K    ++ L  K+++TK+ + + D
Sbjct: 556 QSGILRKLRDLQEQYSRTDVALAMTLRDVTLYIGKDHITLLDLDPKDMNTKMSKWERD 613


>SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein
           Bms1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1121

 Score = 27.1 bits (57), Expect = 4.7
 Identities = 18/65 (27%), Positives = 34/65 (52%)

Query: 334 RDEEIAKSTSDMIQENGYLTPEKLSQIANVSVLLARERLFTTERLGLACRDESIEGLAFY 393
           + E    S SD++  +      K+S++AN S+    E+L  +E   L+ + E+ + LA  
Sbjct: 532 KGESAKSSESDLVVSDDEEDFFKVSKVANESISSNHEKLMESESDRLSKKWENPQLLAQL 591

Query: 394 PNRFL 398
            +RF+
Sbjct: 592 KSRFI 596


>SPAC2C4.09 |||DUF1640 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 216

 Score = 27.1 bits (57), Expect = 4.7
 Identities = 13/42 (30%), Positives = 21/42 (50%)

Query: 228 DTVRFKSYLMSLGIDDPVTRDAFRSDSEYYLGLAQQVSDMMV 269
           +++RF   L   GIDD  +    R  S  Y  + +++SD  V
Sbjct: 42  NSLRFVRVLQEAGIDDKKSETLMRLISNVYSDMHEKISDFSV 83


>SPBC21.03c |||DUF55 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 239

 Score = 26.2 bits (55), Expect = 8.2
 Identities = 11/30 (36%), Positives = 19/30 (63%)

Query: 5   EYIEARLFDGENYLKRDKNVKIYDGDDKTQ 34
           E I+ARL + E   KR + + + DG++K +
Sbjct: 178 EIIKARLQEEEKVAKRKRKLNLSDGENKAK 207


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.319    0.137    0.397 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,703,593
Number of Sequences: 5004
Number of extensions: 68441
Number of successful extensions: 197
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 191
Number of HSP's gapped (non-prelim): 8
length of query: 401
length of database: 2,362,478
effective HSP length: 74
effective length of query: 327
effective length of database: 1,992,182
effective search space: 651443514
effective search space used: 651443514
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 55 (26.2 bits)

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