Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001157-TA|BGIBMGA001157-PA|IPR001781|LIM, zinc-binding
         (226 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    64   2e-11
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    38   0.002
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      33   0.026
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    28   0.98 
SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    26   3.9  
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch...    26   5.2  
SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyc...    26   5.2  
SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyc...    25   9.1  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    25   9.1  

>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 35/124 (28%), Positives = 59/124 (47%), Gaps = 8/124 (6%)

Query: 34  QICAGCSKVITERYLLKALDQLWHEDCLKCGCCDCRLGEVGHTL---YTRANLILCKRDY 90
           +ICA C +VI+ +Y+ +AL  ++H +C +C  C+  +      +        + LC+ DY
Sbjct: 114 KICASCGQVISGQYV-RALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDY 172

Query: 91  LRLFGNTGYCAACNKVIPAFEMVMRARSNVYHLECFACQQCNHRFCVGDRFYLCENKILC 150
            R       CA+C   +  + +   A +  +H+E F C  C   F   D +Y  E K+ C
Sbjct: 173 FRRLDLL--CASCGMALRGYYIT--ALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYC 228

Query: 151 EYDY 154
            Y Y
Sbjct: 229 HYHY 232



 Score = 48.4 bits (110), Expect = 9e-07
 Identities = 28/72 (38%), Positives = 37/72 (51%), Gaps = 6/72 (8%)

Query: 91  LRLFGNTGYCAACNKVIPAFEMVMRARSNVYHLECFACQQCN----HRFCVGDRFYLCEN 146
           +R   ++  CA+C +VI      +RA  N+YHLECF C  CN     +F   D   L + 
Sbjct: 107 VRKVSSSKICASCGQVISG--QYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQ 164

Query: 147 KILCEYDYEERL 158
             LCE DY  RL
Sbjct: 165 VPLCETDYFRRL 176


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 29/117 (24%), Positives = 42/117 (35%), Gaps = 10/117 (8%)

Query: 35  ICAGCSKVITERYL-LKALDQLWHEDCLKCGCCDCRLGEVGHTLYTRANLILCKRDYLRL 93
           +C  C +    R   +     +WH+DC  C  CD  L      L   ++     R     
Sbjct: 18  VCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSD----GRPVCSS 73

Query: 94  FGNTGYCAACNKVIPAFEMVMRARSNVYHLECFACQQCNHRFCVGDRFYLCENKILC 150
             +T  C AC   I  + ++    S  YH ECF C  C  +  +   F      I C
Sbjct: 74  CAHT--CTACRMRIKDYALMSGYDS--YHRECFRCHDCRKQI-IDSNFKRDNRTIFC 125


>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 33.5 bits (73), Expect = 0.026
 Identities = 19/59 (32%), Positives = 26/59 (44%), Gaps = 4/59 (6%)

Query: 100 CAACNKVIPAFEMVMRARSNVYHLECFACQQCNHRF-CVGDRFYLCENKILCEYDYEER 157
           C +C   + A  ++  A     H +CF C  C+     VG  FY  E K  C  DY E+
Sbjct: 258 CHSCGGSLRAGRIIS-ASGKKLHPQCFKCDTCSQNLEHVG--FYYREGKFYCHLDYHEQ 313



 Score = 32.7 bits (71), Expect = 0.045
 Identities = 27/116 (23%), Positives = 47/116 (40%), Gaps = 9/116 (7%)

Query: 36  CAGCSKVITERYLLKALDQLWHEDCLKCGCCDCRLGEVGHTLYTRANLILCKRDYLRLFG 95
           C  C   I ++  +   +  +HE+   C  C   +  V      R +L  C+  Y   + 
Sbjct: 318 CKHCKTPIEDQ-AVHINNDWFHENHHFCAGCS-EVFNVNIPCIYRDDLYWCQTCYDNKYA 375

Query: 96  NTGYCAACNKVIPAFEMVMRARSNVYHLECFACQQCNHRFCVGDR-FYLCENKILC 150
               C  C K  P   + ++     YH +C+ C  CN    +GD  +++ EN  +C
Sbjct: 376 VK--CKKCRK--PILGISVKGSDGEYHSQCWTCGACN--ALLGDEGYFMIENTPIC 425


>SPBC28E12.03 |rga4||GTPase activating protein
           Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 28.3 bits (60), Expect = 0.98
 Identities = 11/38 (28%), Positives = 18/38 (47%), Gaps = 1/38 (2%)

Query: 97  TGYCAACNKVIPAFEMVMRARSNVYHLECFACQQCNHR 134
           T +C  C + +P+   V       +H +CF C  CN +
Sbjct: 21  TCFCIKCWESVPSTSQVWFG-GKCWHSDCFKCVNCNKK 57



 Score = 26.2 bits (55), Expect = 3.9
 Identities = 8/15 (53%), Positives = 10/15 (66%)

Query: 56 WHEDCLKCGCCDCRL 70
          WH DC KC  C+ +L
Sbjct: 44 WHSDCFKCVNCNKKL 58


>SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 646

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 10/29 (34%), Positives = 15/29 (51%)

Query: 80  RANLILCKRDYLRLFGNTGYCAACNKVIP 108
           R   +L  RDY    GN+ +C   N ++P
Sbjct: 243 RTLFLLLIRDYSLFHGNSSHCMLANSMLP 271


>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
           Y|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1002

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H  +   H+PPPP++
Sbjct: 209 PPPMHH--KPGEHMPPPPMH 226



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 222 PPPMHHEPGE--HMPPPPMH 239



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 235 PPPMHHEPGE--HMPPPPMH 252



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 248 PPPMHHEPGE--HMPPPPMH 265



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 261 PPPMHHEPGE--HMPPPPMH 278



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 274 PPPMHHEPGE--HMPPPPMH 291



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 287 PPPMHHEPGE--HMPPPPMH 304



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 300 PPPMHHEPGE--HMPPPPMH 317



 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/20 (45%), Positives = 14/20 (70%), Gaps = 2/20 (10%)

Query: 167 PPPLAHLKRQTTHLPPPPVN 186
           PPP+ H   +  H+PPPP++
Sbjct: 313 PPPMHHEPGE--HMPPPPMH 330


>SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 852

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 10/33 (30%), Positives = 17/33 (51%)

Query: 164 AYNPPPLAHLKRQTTHLPPPPVNITFVFNVIFY 196
           A+ PPP   +KR+   +P P  +    F ++ Y
Sbjct: 126 AHRPPPTLGMKRKVDFIPRPLYDPADEFAIVLY 158


>SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1238

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 4/35 (11%)

Query: 101  AACNKVIPA--FEMVMRARSNVYHLECFA--CQQC 131
            A+C+ V+P+  FEM +R   N   L C    C++C
Sbjct: 1038 ASCDMVLPSEEFEMHLRGHLNNIRLNCEVSNCKKC 1072


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1811

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 16/70 (22%), Positives = 30/70 (42%), Gaps = 2/70 (2%)

Query: 82   NLILCKRDYLRLFGNTGYCAACNKVIPAFEMVMRARSNVYHLECFACQQCNHRFCVGDRF 141
            +LI C   + +L GN  +C +C  ++   E  +         E    ++    + +   F
Sbjct: 1348 DLISCITKFAKLNGNQKFCLSCVDMLKNLEHELIKHLKHMKKESVYSKKLEEEYWL--PF 1405

Query: 142  YLCENKILCE 151
             L  N+I+CE
Sbjct: 1406 LLSFNEIICE 1415


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.328    0.141    0.473 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,055,618
Number of Sequences: 5004
Number of extensions: 43419
Number of successful extensions: 152
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 129
Number of HSP's gapped (non-prelim): 23
length of query: 226
length of database: 2,362,478
effective HSP length: 70
effective length of query: 156
effective length of database: 2,012,198
effective search space: 313902888
effective search space used: 313902888
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 52 (25.0 bits)