Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001156-TA|BGIBMGA001156-PA|IPR010097|Malate
dehydrogenase, NAD-dependent, eukaryotes and gamma proteobacteria,
IPR001236|Lactate/malate dehydrogenase
         (344 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_4308| Best HMM Match : Ldh_1_C (HMM E-Value=2.6e-31)                73   3e-13
SB_32263| Best HMM Match : Ldh_1_C (HMM E-Value=8.80015e-42)           46   6e-05
SB_19075| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.5  
SB_16223| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   7.3  

>SB_4308| Best HMM Match : Ldh_1_C (HMM E-Value=2.6e-31)
          Length = 368

 Score = 73.3 bits (172), Expect = 3e-13
 Identities = 49/150 (32%), Positives = 79/150 (52%), Gaps = 19/150 (12%)

Query: 166 SKMFGITHIDTSRSRSFVAKALKINAQEIQVPVIGGHSDKTIIPLFSNLTPRNYRIEPCQ 225
           +K+ G+T +D  R+ +FVA+A  ++   I++PVIGGHS  TI+PL S  TP N      +
Sbjct: 108 AKILGVTTLDIVRAHTFVAEAKGLDVNNIKIPVIGGHSGVTILPLLSQTTP-NVTFTQDE 166

Query: 226 ADMLTRLVRKAGTEVVMHKHGAESSTLAMAWSINEFIDIILDAILGYRVVVNCYTANSNF 285
            + LT  ++ AGTEVV  K GA                 +++A+ G + VV C    S+ 
Sbjct: 167 LEKLTDRIQNAGTEVVNAKAGA-----------------VIEALNGKKDVVQCAFIKSDL 209

Query: 286 -GTKFFAGPTSVGPHGIIQACCHFPMSEFE 314
               +FA P  +G +G+ +      +S++E
Sbjct: 210 TEAGYFATPVVLGTNGVEKNLGMGKLSDYE 239



 Score = 44.0 bits (99), Expect = 2e-04
 Identities = 24/84 (28%), Positives = 43/84 (51%)

Query: 48  NSKVSKLHLYDDDDRIKGTELELSNLPGGPSVSAFIGDTNLEQAIHSSNLVVMVSRMPRK 107
           +S+  K HL          EL    +   P V++  G  +L+ A+   ++V + + +PRK
Sbjct: 25  SSQTYKNHLIKSYVPTPSWELLSQPITEQPQVTSHQGPDDLKAALEGCSVVAIPAGVPRK 84

Query: 108 PGNTREQMLGANAPLIQRLCKAIA 131
           PG TR+ +   NA +++ L +A A
Sbjct: 85  PGMTRDDLFNTNASIVKNLSEACA 108


>SB_32263| Best HMM Match : Ldh_1_C (HMM E-Value=8.80015e-42)
          Length = 411

 Score = 45.6 bits (103), Expect = 6e-05
 Identities = 34/147 (23%), Positives = 66/147 (44%), Gaps = 7/147 (4%)

Query: 65  GTELELSNLPGGPSVSAFIGDTNLEQAIHSSNLVVMVSRMPRKPGNTREQMLGANAPLIQ 124
           G  LEL +    P +   +   + E+A    ++ ++V  MPR+ G  R+ +L ANA + +
Sbjct: 130 GVVLELQDC-AIPLLHEVVATDDAEKAFTDIDVAILVGAMPRREGMERKDLLSANAKIFE 188

Query: 125 RLCKAI-AAQNQEAFVAISTNPINYLIPFASTVMYKYGCYNPSKMFG-ITHIDTSRSRSF 182
              KA+     +   + +  NP N        +  ++    P + F  +T +D +R  S 
Sbjct: 189 AQGKALDKVAKKTCKILVVGNPAN----TNCLIAQRWAPSIPKENFSCLTRLDQNRGGSQ 244

Query: 183 VAKALKINAQEIQVPVIGGHSDKTIIP 209
           +A  L +   +++  +I G+   T  P
Sbjct: 245 IAGRLNLRVDQVKNVIIWGNHSATQYP 271


>SB_19075| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 6500

 Score = 29.1 bits (62), Expect = 5.5
 Identities = 24/94 (25%), Positives = 40/94 (42%), Gaps = 2/94 (2%)

Query: 39   SYVSLFLKRNSKVSKLHLYDDDDRIKGTELELSNLPGGPSVSAFIGDTN--LEQAIHSSN 96
            SY+    K  S +S    +   + +K     +S +PG P+V   +       E    S+ 
Sbjct: 4093 SYIGFGDKTTSPISFKDKFPSAEDLKSRIDVISLVPGRPNVLEALDKARNEFETGGRSNA 4152

Query: 97   LVVMVSRMPRKPGNTREQMLGANAPLIQRLCKAI 130
              ++V  + ++  NT EQ+  A  PL  R  K I
Sbjct: 4153 KKILVIVVDKRSPNTNEQIKDAVTPLADRDVKVI 4186


>SB_16223| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 519

 Score = 28.7 bits (61), Expect = 7.3
 Identities = 12/33 (36%), Positives = 19/33 (57%)

Query: 299 HGIIQACCHFPMSEFESYLLNRCIPILSREVLE 331
           HG +Q   +FP+  F    L   +P+L RE+L+
Sbjct: 185 HGQLQEATNFPLRPFVIPFLKANLPLLQRELLQ 217


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.321    0.135    0.397 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,726,382
Number of Sequences: 59808
Number of extensions: 408067
Number of successful extensions: 725
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 720
Number of HSP's gapped (non-prelim): 5
length of query: 344
length of database: 16,821,457
effective HSP length: 83
effective length of query: 261
effective length of database: 11,857,393
effective search space: 3094779573
effective search space used: 3094779573
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 60 (28.3 bits)