Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001156-TA|BGIBMGA001156-PA|IPR010097|Malate
dehydrogenase, NAD-dependent, eukaryotes and gamma proteobacteria,
IPR001236|Lactate/malate dehydrogenase
(344 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|ch... 157 2e-39
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 60 3e-10
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 30 0.55
SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharom... 28 1.7
SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces p... 27 3.9
SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch... 27 3.9
SPAC1687.04 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 6.8
SPBC18E5.10 |||iron sulfur cluster assembly protein |Schizosacch... 26 9.0
SPBP35G2.11c |||transcription related zf-ZZ type zinc finger pro... 26 9.0
>SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 341
Score = 157 bits (381), Expect = 2e-39
Identities = 95/318 (29%), Positives = 166/318 (52%), Gaps = 9/318 (2%)
Query: 24 RNVQVSVIGAASDIGSYVSLFLKRNSKVSKLHLYDDDDRIKGTELELSNLPGGPSVSAFI 83
R +V+V+GA IG +S+ LK N KVS+L L+D G ++ ++ +V +
Sbjct: 27 RAFKVAVLGAGGGIGQPLSMLLKLNDKVSELALFDIRGA-PGVAADIGHINTTSNVVGYA 85
Query: 84 -GDTNLEQAIHSSNLVVMVSRMPRKPGNTREQMLGANAPLIQRLCKAIAAQNQEAFVAIS 142
D LE+A++ +++V++ + +PRKPG TR+ + NA +++ L A EA +
Sbjct: 86 PDDKGLEKALNGADVVIIPAGVPRKPGMTRDDLFATNASIVRDLAFAAGETCPEAKYLVV 145
Query: 143 TNPINYLIPFASTVMYKYGCYNPSKMFGITHIDTSRSRSFVAKALKINAQEIQVPVIGGH 202
TNP+N +P + + G + P +FG+T +D+ R+ F ++ A+ + +PV+GGH
Sbjct: 146 TNPVNSTVPIFKKALERVGVHQPKHLFGVTTLDSVRASRFTSQVTNGKAELLHIPVVGGH 205
Query: 203 SDKTIIPLFSNLTPRNYRIEPCQADMLTRLVRKAGTEVVMHKHGAESSTLAMAWSINEFI 262
S TI+PL L+ + + D L ++ G EVV K GA S+TL+MA++
Sbjct: 206 SGATIVPL---LSQGGVELTGEKRDALIHRIQFGGDEVVKAKAGAGSATLSMAYAGARMA 262
Query: 263 DIILDAILGYRVVVNCYTANS----NFGTKFFAGPTSVGPHGIIQACCHFPMSEFESYLL 318
+L A+ G V C S + G FFA ++G G+ ++++E LL
Sbjct: 263 SSVLRALAGESGVEECTFVESPLYKDQGIDFFASRVTLGKDGVDTIHPVGKINDYEESLL 322
Query: 319 NRCIPILSREVLEGEKYV 336
+ L + + +GE++V
Sbjct: 323 KVALGELKKSITKGEQFV 340
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 60.5 bits (140), Expect = 3e-10
Identities = 39/187 (20%), Positives = 93/187 (49%), Gaps = 11/187 (5%)
Query: 24 RNVQVSVIGAASDIGSYVSLFLKRNSKVSKLHLYD-DDDRIKGTELELSNL-PGGPSVSA 81
+++++ ++GA + +GS + L + +++ + D + + +G ++L++ P
Sbjct: 19 KSIKIVIVGAGN-VGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAAPLSHETRV 77
Query: 82 FIGDTNLEQAIHSSNLVVMVSRMPRKPGNTREQMLGANAPLIQRLCKAIAAQNQEAFVAI 141
++GD + + VV+ + +KPG TR +L AN + + + + + ++A + +
Sbjct: 78 YLGDY---KDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLV 134
Query: 142 STNPINYLIPFASTVMYKYGCYNPSKMFGI-THIDTSRSRSFVAKALKINAQEIQVPVIG 200
+TNP++ L + K + ++ G T IDT+R + + K ++ Q + +IG
Sbjct: 135 ATNPVDVL----TYATLKLTGFPAERVIGSGTIIDTARFQYLIGKLYGLDPQSVNADIIG 190
Query: 201 GHSDKTI 207
H D +
Sbjct: 191 EHGDSEL 197
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 29.9 bits (64), Expect = 0.55
Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Query: 112 REQMLGANAPLIQ-RLCKAIAAQNQEAFVAISTNPINYLIPFASTVMYKYGCYNPSKMFG 170
RE ++G N ++ +L + + + E +++NP+N I A TV+Y N S + G
Sbjct: 860 RESIIGLNPRTLEAKLLLYLKSLDVEIDALVASNPLNIRIMAAETVVYIIDKLNQSVLEG 919
Query: 171 ITHI 174
T +
Sbjct: 920 KTSV 923
>SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1690
Score = 28.3 bits (60), Expect = 1.7
Identities = 29/122 (23%), Positives = 56/122 (45%), Gaps = 10/122 (8%)
Query: 28 VSVIGAASDIGSYVSLFLKRNSKVSKLHLYDDDDRIKGTELELSNLPGGPSVSAFIGDTN 87
V + ++ G+ + L + +VS++ ++DD D + T+ +NL G +S + N
Sbjct: 1057 VGKVTNVNEAGAILQLPGHMSGRVSRIDMFDDYDILPETKFTRNNLVGVCVLSVDV--PN 1114
Query: 88 LEQAIHSSNLVVMVSRMPRKPGNTREQMLGANAPL-IQRLCKAIAAQ--NQEAFVAISTN 144
+ A+ + N SR +P +++ + + L I +C+ NQ FV I N
Sbjct: 1115 RKVALSARN-----SRTQSQPVEIKDKEINSVDDLKIGDICRGFVCNVANQGLFVTIGHN 1169
Query: 145 PI 146
I
Sbjct: 1170 LI 1171
>SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1028
Score = 27.1 bits (57), Expect = 3.9
Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 4/81 (4%)
Query: 14 LKKNTLILQKRNVQVSVIGAASDIGSYVSLFLKRNSKVSKLHLYDDDDRIKGTELELSNL 73
+KK +++ KR+ +++ + A S GS+ L +S +S L +D + G + +
Sbjct: 216 IKKPSMVDHKRDSEINAVTAVSADGSFSPLTETLSSTISTL----SNDSLDGLSSQKNEF 271
Query: 74 PGGPSVSAFIGDTNLEQAIHS 94
G S S +I + ++ +S
Sbjct: 272 TGFNSNSEWIPNDTVDYICNS 292
>SPCC126.07c |||human CTD-binding SR-like protein rA9
homolog|Schizosaccharomyces pombe|chr 3|||Manual
Length = 571
Score = 27.1 bits (57), Expect = 3.9
Identities = 17/52 (32%), Positives = 24/52 (46%)
Query: 166 SKMFGITHIDTSRSRSFVAKALKINAQEIQVPVIGGHSDKTIIPLFSNLTPR 217
S + I TSR + +L+ + G SD + PLFSNL+PR
Sbjct: 384 SLISNINQPSTSRIDTSFMLSLQHEIKNSHSEASDGTSDVHLTPLFSNLSPR 435
>SPAC1687.04 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 501
Score = 26.2 bits (55), Expect = 6.8
Identities = 23/64 (35%), Positives = 33/64 (51%), Gaps = 9/64 (14%)
Query: 282 NSNFGTKFFAGPTSVGPHGIIQACCH-FPMSEFESYLLNRCIPILSREVLEGEKYVEFME 340
++NFG +FFAG V I + C + SE E +L S+ VL+ E+Y F+
Sbjct: 80 DTNFGHEFFAGAVEVN-ENIWRGCRYILDFSEDEMHLDE------SKIVLD-ERYSLFLT 131
Query: 341 NVKG 344
NV G
Sbjct: 132 NVPG 135
>SPBC18E5.10 |||iron sulfur cluster assembly protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.8 bits (54), Expect = 9.0
Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Query: 283 SNFGTKFFAGPTSVGPHGIIQACCHFPMSE-FESY 316
+N GTK F+ V +I+AC P+ + E+Y
Sbjct: 267 NNRGTKLFSISGEVNEPNVIEACMSIPLKDLIENY 301
>SPBP35G2.11c |||transcription related zf-ZZ type zinc finger
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 397
Score = 25.8 bits (54), Expect = 9.0
Identities = 11/30 (36%), Positives = 15/30 (50%)
Query: 272 YRVVVNCYTANSNFGTKFFAGPTSVGPHGI 301
Y + +C T NS+ T F T PHG+
Sbjct: 156 YDICESCLTDNSHSNTHAFVRITKAYPHGL 185
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.321 0.135 0.397
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,515,341
Number of Sequences: 5004
Number of extensions: 61696
Number of successful extensions: 144
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 136
Number of HSP's gapped (non-prelim): 10
length of query: 344
length of database: 2,362,478
effective HSP length: 73
effective length of query: 271
effective length of database: 1,997,186
effective search space: 541237406
effective search space used: 541237406
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 54 (25.8 bits)
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